• 제목/요약/키워드: tilapia

검색결과 183건 처리시간 0.031초

Tilapia Oreochromis niloticus의 성분화시 Aromatase의 작용시기 (The Timing of Aromatase Action for Sex Differentiation in the Nile Tilapia, Oreochromis niloticus)

  • 권준영;;권혁추
    • 한국발생생물학회지:발생과생식
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    • 제10권3호
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    • pp.197-202
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    • 2006
  • 어류의 체내에서 성분화를 유도하는 물질이 성스테로이드호르몬(sex steroid hormone)이라는 사실이 잘 밝혀져 있으며, 성스테로이드 생합성 효소의 하나인 aromatase도 성분화에 직접적인 역할을 하는 것으로 알려져 있다. 본 연구에서는 유전적으로 암컷인 틸라피아 자어(larvae) 집단을 aromatase 저해제(aromatase inhibitor, AI)인 Fadrozole로 침지 처리하여 초기 발생단계 중 어느 시기에 aromatase가 성분화 유도 작용을 하는 지를 조사하였다. Fadrozole 처리 유무 및 처리 농도의 차이는 부화 자어의 생존율에 유의한 차이를 유발하지 않았다. 하지만, 부화후 11일과 13일째에 고농도의 Fadrozole로 처리한 실험군의 자어는 유전적인 성이 암컷임에도 불구하고 유의하게 높은 비율의 자어가 수컷으로 분화하였다. 이 결과는 틸라피아 부화 자어가 스테로이드 생합성 효소의 저해에 아주 민감하게 반응하며, 이 종에서 aromatase의 주된 작용시기가 예상보다 훨씬 빠른 부화 후 11일 전후라는 사실을 보여준다. 또한 이상의 결과는 단 3시간의 AI 침지 처리가 유전적으로 설정되어 있는 성과 반대방향으로의 성분화를 유도하기에 충분할 정도로 강력함을 의미한다.

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나일틸라피아, Oreochromis niloticus의 비특이적 면역반응에 대한 생약재 투여 효과 (Effect of Dietary Herb Medical Stuff on the Non-specific Immune Response of Nile Tilapia, Oreochromis niloticus)

  • 황미혜;박수일;김이청
    • 한국어병학회지
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    • 제12권1호
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    • pp.7-15
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    • 1999
  • 연구는 어류에 각종 생약재를 투여하였을 때 나타나는 방어 능력의 변화와 질병에 대한 예방 효과를 밝히고자 수행되었다. 실험의 내용은 인삼(Panax ginseng), 구기자(Lycium chinense), 하수오(Polygonum multiflorum), 오미자(Schizandra chinensis)의 첨가 사료를 나일틸라피아에 일정 기간 투여한 후 실험어의 성장, 비특이적 방어 능력 및 생리학적 기능에 미치는 영향을 조사한 것이다. 실험에 사용한 각종 생약재 중에서 체중의 증가는 구기자 3% 투여구가 가장 높게 나타났다. 보체의 살균 능력은 모든 실험구에서 살균력이 12주째에 증강되었고 보체의 용혈 능력도 모든 생약재 투여구에서 대조구 보다 높고 특히 인삼 2% 투여구에서 가장 높게 나타났다. 그러나 라이소자임의 용균능력과 신장의 부착 식세포 활성 산소 생성 능력은 구기자 3% 투여구가 가장 높았다. 그리고 나일틸라피아에 병원성을 지닌 Edwardsiella tarda 균주로 공격 실험한 결과 구기자 3% 투여구가 생존율이 85%로 가상 높았다. Hematocrit, hemoglobin, total protein, glucose, GOT, GPT와 같은 혈액의 생리학적 지표 성분을 분석한 결과 생약재를 첨가한 사료를 먹인 실험어는 대조구와 차이가 없으며 생리적 기능 면에서 나쁜 영향을 주지 않는 것으로 조사되었다. 따라서 인삼, 구기자, 하수오, 오미자 등과 같은 생약재는 그 종류에 따라 나일틸라피아의 성장률, 각종 비특이적 면역력을 증강시키는 능력과 병원성 세균에 대한 저항력의 증강 효과에 차이가 있지만 장기간 투여하여도 생리 기능에 문제를 야기하지 않는 것을 알 수 있었다. 모든 실험 결과를 종합해 보면, 실험에 사용한 각종 생약재 중에서 구기자는 가장 경제적이며, 각종 면역 기능 증강 효과가 뛰어나므로 새로운 사료내 첨가물로서의 충분한 가치가 있으며, 양식 어가에 많은 도움을 줄 것으로 생각된다.

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빨간유글레나, Euglena rubra 건조분말의 사료첨가에 따른 나일틸라피아, Oreochromis niloticus에서의 영향 (Effects of feeding of dried Euglena rubra on Nile tilapia Oreochromis niloticus)

  • 이재근;이한나;김영대;최상훈;박관하
    • 한국어병학회지
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    • 제24권3호
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    • pp.307-313
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    • 2011
  • To examine the effects of Euglena rubra (E. rubra) feeding on Nile tiapia, Oreochromis niloticus were fed diets containing dried powder of E. rubra at 0.5 or 2% for 4 weeks. Growth, selected hematological and non-specific immune parameters were assessed at 2 and 4 weeks. There were no significant changes in body weigh gain and erythrocyte levels. However a significant and diet E. rubra level-related decrease in leukocyte level was noted. Significant increases were observed in respiratory burst activity (nitroblue tetrazolium reduction) and lysozyme activities following E. rubra feeding. These results indicate that E. rubra could be beneficial to fish, but excess feeding could toxic.

Flavobacterium columnare / Myxobolus tilapiae Concurrent Infection in the Earthen Pond Reared Nile Tilapia (Oreochromis niloticus) during the Early Summer

  • Eissa, Alaa E.;Zaki, Manal M.;Aziz, A. Abdel
    • Interdisciplinary Bio Central
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    • 제2권2호
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    • pp.5.1-5.9
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    • 2010
  • Flavobacterium columnare (F. columnare), the dermotropic Gram negative yellow pigmented bacteria was isolated from different sites of skin ulcerations in the Nile tilapia (Oreochromis niloticus) and Nile catfish (Clarias gariepinus) collected from an earthen pond located at an aquaculture station in Sharkiya Province, Lower Egypt during an acute episode of mass kills during the early summer of 2009. An acute infection with F. columnare was behind the emergent event of mass mortalities among both populations. Many of the Nile tilapias exhibited typical signs of hole - in- the head like lesions from which F. columnare together with the myxosporean spore, Myxobolus tilapiae (M. tilapiae) were retrieved. Most of the cohabitating infected Nile catfishes exhibited severe form of saddle back like ulcer. The identities of the retrieved isolates were confirmed using morphological, biochemical and molecular tools. The research lead us to conclude that the two diverse etiological agents (F. columnare and M. tilapiae) under the triggering effect of the abrupt change in the water quality measures (abrupt rise in the water temperature, ammonia, pH, sharp decrease in dissolved oxygen) have synergized together to induce the above mentioned pathology with the consequent reemergence of fish mass mortalities.

Characterization of the Heavy Metals Contaminating the River Nile at El-Giza Governorate, Egypt and Their Relative Bioaccumulations in Tilapia nilotica

  • Morgan, Ashraf M.; Shin, Ho-Chul;Aty, A.M. Abd El
    • Toxicological Research
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    • 제24권4호
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    • pp.297-305
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    • 2008
  • This study was carried out to measure the concentration of heavy metals (Pb, Mn, Cr, Cd, Ni, Zn, and Cu) in water and Bolti fish (Tilapia nilotica) samples collected from Rasheed branch of River Nile, north of El-Giza Governorate, Egypt by atomic absorption spectrophotometry. The investigated districts through which the branch passes include El-Manashi, Gezzaya, El Katta, Abo Ghaleb and Wardan. Based on WHO and FAO safety reference standards, the results of the current study showed that water and fish tissues were found to contain heavy metals at significantly variable concentration levels among the investigated districts. They were polluted with respect to all the metals tested at Gezzaya district. However, the levels of analyzed metals in water and fish tissues were found lower than legal limits in other districts. The heavy metals showed differential bioaccumulation in fish tissues of the different districts as the accumulation pattern (as total heavy metal residues) was district dependant as follow: Gezzaya > Wardan > El Katta > Abo Ghaleb > El Manashi.

Floating Bead Filter에 의한 순환여과식 양식장의 부유고형물 제거와 질산화 (Removal of Suspended Solids and Nitrification by Floating Bead Filter in Recirculating Aquaculture System)

  • 김병진;김성구;서근학
    • 한국수산과학회지
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    • 제36권2호
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    • pp.163-169
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    • 2003
  • The floating bead filter was tested for treatment of aquacultural water in a pilot-scale recirculating aquaculture system. Performance of floating bead filter on the removal of total suspended solids (TSS) and the treatment of nitrogen sourer such as total ammonia nitrogen (TAN), nitrite nitrogen and nitrate nitrogen were evaluated. The system was stocked with Nile tilapia at an initial rearing densities of $5\%\;and\;7\%$ over 30 days. The average TSS removal rates were $43.0\;g/m^2{\cdot}day\;and\;39.5\;g/m^2{\cdot}day$ for rearing density of $5\%\;and\;7\%$, respectively. As rearing density increased from $5\%\;to\;7\%$. the TAN removal efficiency decreased from $22.0\%\;to\;17.7\%$. At the rearing densities of $5\%\;and\;7\%$, the average TAN removal rates and removal efficiencies were $38.8\;g/m^2{\cdot}day,\;15.6\%\;and\;37.8\;g/m^2{\cdot}day.\;17.7\%,$ respectively. The average TAN removal rate was $37.8-38.8\;g/m^3{\cdot}day.$ The oxygen consumption by floating bead filter was higher than theoretical oxygen consumption rate by nitrification.

Response of Pituitary Cells and Tissues to Neurokinin B and F in the Nile tilapia

  • Mun, Seong Hee;Oh, Hyeon Ji;Kwon, Joon Yeong
    • 한국발생생물학회지:발생과생식
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    • 제26권1호
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    • pp.13-21
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    • 2022
  • Neurokinin B (NKB) is a neuropeptide involved in the regulation of reproductive endocrine system of vertebrate animals, including fish. However, the pathway of NKB action in fish has not been clearly elucidated. In order to clarify the effect of NKB and NKF (neurokinin F) on gonadotropic hormone (GTH) gene expression in the pituitary, we studied the changes of LHβ and FSHβ gene expressions by using two different pituitary culture methods (whole pituitary culture or dispersed pituitary cell culture). Pituitaries were removed from mature female and male Nile tilapia. Changes of LHβ and FSHβ gene expressions were measured and compared after the treatment with NKB or NKF peptides at concentrations 0 to 1,000 nM. Expression of GTH genes in the whole pituitary cultures treated with NKB or NKF peptides did not show significant difference except in female at one concentration when treated with NKF. On the contrary, there were significant changes of GTH gene expressions in the dispersed pituitary cell cultures when treated with NKB and NKF peptides. These results suggest that dispersed pituitary cell culture is more relevant than whole pituitary culture in studying the function of pituitary, and that NKB and NKF could act directly on the pituitary to regulate the expression of GTH genes.