• 제목/요약/키워드: three dog days

검색결과 80건 처리시간 0.032초

발치와의 육아조직 이식이 치근이개 결손부의 재생에 미치는 영향 (Effect of extraction socket granulation tissue graft on the regeneration of horizontal furcation defect)

  • 오목훈;한수부;손성희;양승민;고재승
    • Journal of Periodontal and Implant Science
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    • 제26권3호
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    • pp.735-751
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    • 1996
  • An ultimate goal of periodontal therapy is to stop the disease process and to regenerate a functionally-oriented periodontium destroyed as a result of periodontal disease. The purpose of this study was to observe the effect of grafting granulation tissue obtained from extraction socket on the regeneration of horizontal furcation defect. Six dogs were used in this study. All mandibular first and third premolars were extracted. At 2, 3, and 5 days after extraction, tissues were obtained from extraction socket of 1 mongrel dog and examined by light microscope. Granulation tissue obtained at 5 days after extraction was chosen as the graft material. Five days later, horizontal furcation defects were created surgically at mandibular second and fourth premolars in the right and left side of the 5 beagle dogs. The entrance area of the artificially prepared "key hole" defects were about $3\;4mm^2$. By random selections, 2 exposed furcation defects were grafted with granulation tissue obtained from extraction socket as experimental group and 1 furcation defect was as control. The flaps were replaced to their original position and sutured with 4-0 chromic cat-gut. Three dogs were sacrificed 4 weeks and two dogs 8 weeks after surgery, and the prepared specimens were examined by light microscope. At 4 weeks, furcations were filled with epithelial lining and fibrous connective tissue infiltrated with chronic inflammatory cells. New bone formation was observed in all groups. Only experimental group showed new cementum formation. At 8 weeks, new cementum, functional arrangement of new PDL fiber, root resorption, and some ankylotic union of newly formed alveolar bone and root surface were observed in all groups. Experimental group showed that epithelial downgrowth was inhibited and new bone formation was more active compared to control. The success rate of the furcation defect healing was higher in experimental group than control. These results suggested that grafting of granulation tissue obtained from extraction socket which combined with reconstructive periodontal flap surgery may promote periodontal regeneration of horizontal furcation defect.

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유성견에서 상악골 급속확장 후 정중구개봉합부에서의 골조직 변화 (THE BONE CHANGES OF THE MIDPALATAL SUTURE AFTER RAPID PALATAL EXPANSION IN YOUNG ADULT DOGS)

  • 박창욱;윤영주;김광원
    • 대한치과교정학회지
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    • 제27권3호
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    • pp.391-399
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    • 1997
  • 급속정중구개확장 후 봉합부에서의 골조직 변화를 관찰하기 위해 유성견 4마리중 1마리는 대조군으로, 3마리는 실험군으로 사용하여 상악골에 급속정중이개장치를 장착한 후 매일 아침, 저녁으로 2회전($180^{\circ}$)씩 활성화시켜 8일간 정중이개를 시킨 직후, 보정 14일후, 보정 28일후에 각각 희생시켜 정중구개봉합부를 절취하여 각각 통법에 따라 H-E 염색, Villanueva 염색, 염기성 인산분해효소 염색, tartrate저항성 산성분해효소 염색을 시행하여 봉합부를 광학현미경으로 관찰하곤 다음과 같은 결과를 얻었다. 1. 정중구개봉합 확장 직후, 교원섬유 다발은 신장되었고 조골세포는 비활성화된 양상으로 소수 관찰되었으며 소수의 골양조직도 관찰되었다. 또한 수평절단 표본에서는 TRAP 염색에 양성반응을 보이는 세포가 소수 관찰되었으나 전두절단 표본에서는 구개골의 성장방향에 따라 비강에 인접한 부위에서 TRAP 염색에 양성반응을 보이는 파골세포들이 다수 관찰되었다. 2. 정중구개봉합 확장 14일후, 교원섬유다발은 신장되었고 ALP 염색에 양성반응을 보이는 조골세포가 소수 관찰되었으며, 수평절단 표본에서는 TRAP 염색에 양성반응을 보이는 세포가 거의 관찰되지 않았으나 전두절단 표본에서는 구개골의 성장방향에 따라 비강에 인접한 부위에서 TRAP 염색에 양성반응을 보이는 파골세포들이 다수 관찰되었다. 3. 정중구개봉합 확장 28일후, 교원섬유다발은 대조군과 유사한 양상으로 배열되었고 ALP 염색에 양성반응을 보이는 조골세포가 다수 관찰되었으며 많은 양의 미성숙골이 형성되었고, 수평절단 표본에서는 TRAP 염색에 양성반응을 보이는 세포가 거의 관찰되지 않았으나 전두절단 표본에서는 구개골의 성장방향에 따라 비강에 인접한 부위에서 TRAP 염색에 양성반응을 보이는 파골세포들이 다수 관찰되었다. 이상의 결과를 종합해볼 때, 정중구개봉합부를 급속확장시킨 후 신생골 형성은 보정 14일이 지난 후부터 관찰되기 시작하였으며, 보정 28일이 지난 후에는 신생골형성이 훨씬 왕성하게 진행되었음을 관찰할 수 있었다.

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밀웜 펠렛과 반려견 사료의 혼합급여가 반려견의 단백질 소화율과 분변성상에 미치는 영향 (Effect of Mixing Mealworm Pellets with Canine Diets on Protein Digestibility and Fecal Properties in Companion Canine)

  • 김경민;정연우
    • 한국환경과학회지
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    • 제32권12호
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    • pp.991-994
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    • 2023
  • This study aimed to investigate the effects of feeding mealworm pellets on protein digestibility and fecal composition of companion canines. The canine breeds used in the specification test were Maltese (average weight 2.0 kg) and Poodle (average weight 2.5 kg), with a total of six canines, three of each breed, without discriminating between males or females. The control feed for the two canines was based on 100 g of commercially available general feed, to which the dogs were allowed to adapt for approximately 3 d. Ony on Day 0 of the experiments, dogs were fed twice, in the morning and afternoon. One day later, the treated group was 80 g of regular feed with 20 g of mealworm pellets added, and the dog was fed for 1 to 7 d in the same manner as the control group. The addition of mealworm pellets increased the protein digestibility in both canine breeds, however, the differences were no significances (p>0.05). Based on the normal stool score of 3, no remarkable changes were observed in stool score from Days 1 to 6, showing that the canines had adapted to the mealworm pellet feed. In particular, on Day 7, feeding mealworm pellet appeared to affect the fecal properties differently between the two breeds. This difference can be owing to differences in the intestinal environment and digestion and absorption ability between the two breeds. Consequently, using mealworm pellets improved the protein digestibility of canines as well as maintained normal fecal properties.

동애등에 펠렛 사료와 혼합급여시 반려견 품종별 분변성상과 소화율에 미치는 영향 (Beneficial Effect of Feeding Hermetia illucens Pellets with Canine Diet on Stool Score and Digestibility of Each Canine Breed)

  • 김경민;정연우
    • 한국환경과학회지
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    • 제32권12호
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    • pp.995-998
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    • 2023
  • This study aimed to determine the effect of feeding Hermetia illucens (H.illucens) pellets with canine diets on fecal properties and digestibility. The canine breeds used in this experiment were nine dogs without discriminating between genders: 3 Maltese (average weight 2.0 kg), 3 Chihuahua (average weight 1.0 kg), and 3 Poodles (average weight 2.5 kg). As canine diets, 70 g of regular diets were mixed with 30 g of H.illucens pellets, and 100 g was weighed on a scale. Dog were fed the experimental diets once a day. No significant changes were observed in the fecal properties in the three types of canines fed a mixture of pellets and canine diets at Days 0 and 7 (p>0.05). At Day 0, the fecal properties of all canines appeared in a less solid state, with scores ranging from 2.00 to 2.22; however, at Day 7, the fecal properties improved to normal (3.00 to 3.33). In addition, the results for nutrient digestibility showed that dry matter and crude fat digestibility were significant (p<0.05); however, crude protein and crude ash digestibility were not affected (p>0.05). In conclusion, these results suggest that feeding a mixture of H.illucens pellets and canine diets to companion canines has beneficial effects on fecal properties and digestibility without adverse effect.

내시경과 Cerclage Wire를 이용한 식도내 낚시바늘 제거: 증례보고 (Endoscopic Retrieval of Esophageal Fishhooks Using Cerclage Wire: A Case Report)

  • 김영기;엄미영;서유진;하미현;왕지환;정인조;장홍희;이희천;조규완;이효종;연성찬
    • 한국임상수의학회지
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    • 제24권4호
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    • pp.622-626
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    • 2007
  • 1년 6개월령의 수컷 비글견이 3일간의 연하통증과 식욕부진으로 내원하였다. 환축은 병력청취와 방사선학적 검사결과 낚시바늘에 의한 식도와 위내 이물로 진단되었다. 위식도 내시경을 시행한 결과 하나의 낚시바늘은 흉부식도의 심장기저 부에 나머지 하나의 낚시바늘은 위 분문부에 위치하고 있었다. 그리고 두 낚시바늘은 하나의 낚싯줄로 서로 연결되어 있었다. 연결된 낚싯줄을 끊고 위내 낚시바늘을 제거하기 위해 위 절개술을 시행하였다. 위 절개술 후 식도내 바늘을 제거하기 위해 세 갈래, 다섯 갈래 내시경 포셉과 생검 포셉을 이용한 내시경적 제거를 시도하였으나 낚시바늘이 식도의 점막에 깊게 박혀있어 견인에 실패하였다. 낚시바늘을 더욱 단단히 잡아줄 수 있는 장치를 위해 정형외과용 스테인리스 와이어(16G)를 올가미 포셉 형태로 제작한 뒤 내시경을 통한 시야가 확보된 상태에서 바늘에 걸어 제거하는데 성공하였다. 시술 10일 후 환축은 회복되어 퇴원조치 되었다.

생체실험을 통한 좌심실보조기의 평가 (Evaluation of Left Ventricular Assist Device through In Vivo Experiments)

  • 박성근;원용순;정필섭;최진욱;김인영;이규백;민병구
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1993년도 추계학술대회
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    • pp.89-92
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    • 1993
  • In this article, we present and analyze the results of the three consecutive in vivo experiments of the LVAD to evaluate the function of the LVAD and the adverse effects on living animals. We applied the LVAD consecutively to three mongrel dogs and the circulation of the blood was assisted under the anesthesia. We used in general both the asynchronous mode and the synchronous mode to drive the LVAD. During the experiments we monitored the dogs with a polygraph to evaluate the function of the LVAD and the additional effects on the natural hearts. We also examined several clinical pathologic tests in order to see the effects of the LVAD to the red blood cells and the other internal organs. The dogs survived for two to there days. The LVAD assisted the circulatory system at the maximum assist flow rate of 3.0 1/min. Although the red blood cells of the dogs had mechanical damages by the LVAD to result in the hemolysis, the degree of the hemolysis was not so high and the damages caused by the hemolysis on the dogs were not serious. The myocardium of the first dog was gradually worsened and eventually failed. The damage of the myocardium was due to the asynchronous driving mode of the LVAD. The other organs did not have serious damages due to the application of the LVAD. The main purpose of this paper is to evaluate the results of the in vivo experiments of the LVAD and to find better ways to the application of the LVAD to human beings.

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Beagle Dog에서 cis-Malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1,3-dioxolane]platinum(II)(SKI 2053R)의 아급성독성시험에 관한 연구 (Subacute toxicity of cis-Malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1,3-dioxolane]platinum(II)(SKI 2053R) in Beagle Dogs)

  • 이영순;강경선;신동진;조재진;김형욱;김배환;임윤규
    • Toxicological Research
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    • 제8권2호
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    • pp.235-253
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    • 1992
  • A subacute toxicity study of cis-Malonato[(4R,5R)-4,5-bis(aminomethyl)-2-isopropyl-1,3-dioxolane]platinum(II)(SKI 2053R) was carried out to obtain information on its toxicological profiles, and to determine the maximum tolerated dose in beagle dogs. Four groups of beagle dogs (2M and 2F per group, 0,0.5,1.0,2.0mg/kg/day)were given 15 i.v. injections of SKI 2053R. In order to compare the toxic effects of SKI 2053R with those of cisplatin, one group was treated with cisplatin(0.7mg/kg/day)according to the same treatment schedule. The dosing schedule was divided into 3 courses of 5 consecutive days with 23-day dose-free intervals between each course. After completion of the treatments, remaining dogs were necropsied under established guidelines. Three of four dogs in the high dose group and one of four dogs in the middle dose group treated with SKI 2053R died of hypovolemic shock secondary to hemorrhagic and ulcerative enterocolitis. No toxicity-related mortality occurred in the low dose group of SKI 2053R. No survivor was observed in the group of cisplatin. Clinical signs including vomiting, diarrhea, anorexia and loss body weight were apparent in dogs given either cisplatin or high and middle doses of SKI 2053R. Severe thrombocytopenia and leukocytopenia were observed in the high dose group of SKI 2053R and cisplatin-treatment group, while toxicities as bone marrow suppression were reversible. The significant elevation of serum ALP values in group of SKI 2053R(2.0 mg/kg/day and 1.0mg/kg/day) and cisplatin(0.7mg/kg/day)was observed. Slight proteinuria waa observed in high and middle dose level groups of SKI 2053R. In histopathological examinations, pathological alterations of liver, kidney and spleen were noted dose-dependantly in dogs treated with SKI 2053R, and there was no overt sign of toxicity in low dose group of SKI 2053R. Compared to SKI 2053R, more severe durg-related toxicities occurred in dogs treated with cisplatin. It waw estimated that maximum tolerated dose of SKI 2053R in this treatment schedule was 0.5~0.7mg/kg/day. In conclusion, overall toxic potential of SKI 2053R was approximately 3 times lower than that of cisplatin with respect of lethality.

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개의 어깨 관절에서 박리성 골연골염의 진단과 치료를 위한 탐색적 관절내시경의 적용 (Exploratory Arthroscopic Treatment to Diagnose and Treat Osteochondritis Dissecans of the Shoulder Joint in a Dog)

  • 김충섭;이해범;최을수
    • 한국임상수의학회지
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    • 제30권2호
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    • pp.146-149
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    • 2013
  • 6개월 된 수컷 Great Pyrenees (35 kg)가 왼쪽 앞다리에 2주 동안 지속된파행으로 전북동물의료센터에 내원하였다. 파행은 병원 내원 3일 전부터 더욱 심해졌다고 한다. 신체검사 상에서 환자는 왼쪽 어깨 부분에 통증을 보였고, 보행 검사에서 파행을 보이고 있었다. 방사선 검사와 신경계 신체검사에서는 유의적인 소견을 보이지 않았다. 의학적 기왕력과 보행검사를 토대로 박리성 골연골염을 의심하였다. 어깨관절에 대한 확실한 진단을 위해서, 탐색적 관절내시경 수술이 시행되었다. 미미한 두갈래근건초염과, 활막염, 그리고 상완골 머리 후내측에 위치한 박리성 골연골염 병변이 관찰되었다. 연골편의 제거, 연골하골의 변연절제가 관절내시경과 전기 버(electric burr)를 사용하여 실시되었다. 환자는 수술 다음 날에 퇴원하였다. 2주 후, 환자는 술 부를 핥아서 생긴 염증과 피하조직에 생긴 장액종으로 인해 내원하였다. 항생제 처치와 무균적 붕대가 적용되었고, 장액종은 수술적으로 제거되었으며, 관절내시경을 실시한 11주 후 환자의 보행은 정상이었다. 관절내시경은 관절낭 안쪽의 구조물 관찰이 가능하기 때문에 진단이 어려운 관절질환에 유용하게 사용될 수 있을 거라 사료된다.

"고사십이집(攷事十二集)"의 조리가공에 관한 분석적 연구(2) (A Study on the Cooking in 'The Kosa-sibi Jip' (2))

  • 김성미
    • 동아시아식생활학회지
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    • 제4권3호
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    • pp.1-19
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    • 1994
  • In this paper, twenty-five kinds of food presented in Sooljip(戌集) 5 and 6 of Food collections of 'Kosa-sibi Jip(攷事十二集)' have been classified into four : Staple food, subsidiary food, Tuck(rice cake) and Han-gwa(Korean confectionery), and Tang-jng and tea. Cooking processes have been examined and scientifically analyzed in terms of cooking, Fourteen kinds of Jook (thick gruel with cereal) as well as Urak-Jook were presented among the methods of making Jook, one of staple foods. Milk and ground rice were boiled together into Urak-Jook, which was nutritious because of carbohydrate, added to milk. Hong-sa Myun was mode of ground shrimps, ground bean, ground rice and flour which were kneaded together. It was a nutritiously balanced food. Nineteen kinds of Kimchi presented in this book were classified by the recipes. The five of Jook-soon Ja, U-so Ja, Tam-bok Ja and Jo-gang were made by adding red malt and cereals(boiled rice or candies). Jo-gang, Jo-ga and Jo-gwa-chae were made by adding salt and rice wine. With salt and fermenters added, eight were made. Chim-jup-jeo-ga was made by adding Jang(soy-bean sauce) and the inner chaff of wheat instead of salt. The four of Ka-za-san, Hwang-gwa-san, Tong-gwa-san and Jo-gang were made by adding salt and vinegar. Jo-gang was made by adding salt, rice wine, residue of rice wine and candies. The four of Kae-mal-ga, Ku-cho-chim-chae, Un-gu-hwa and Suk-hwa-chim-chim-chae were made by adding salt and spices. San-got-Kimchi was made without salt. San-got-Kimchi and Suk-hwa-chim-chae were made originally in Korea. Suk-hwa-chim-chae, in particular, was first classified as a kind of Kimchi in this book and oysters were added, which is notable. Pork could be preserved longer when smoked oven the weak fire of thatch ten days and nights. Dog meat was sauced and placed on the bones in a pot. A porcelain was put on the top of the pot. Flour paste sealed the gap between the porcelain and the pot. Some water was poured into the porcelain, and the meat was steamed, with two or three thatched sacks burned, which was a distilled dry steaming. This process has been in use up to now. Various cooking methods of chicken were presented from in Umsik-dimi-bang to in Chosun Musang Sinsik Yori Jebup. These methods were ever present regardless of ages. Such measuring units as Guin(斤) and Nyang(兩) were most frequently used in cooking processes of this book, except in case of Jang(soy bean sauce), vinegar and liquor. Twenty eight kinds of kitchenware and cookers were used, of which porcelains wee most used and pans and sieves followed. The scientific eight cooking methods were as follows. First, salt was refined through saturated solution. Next, it was recommended Hong-sa Myun containing shrimps should not be taken along with pork, which is thought to be a proper diet in terms of cholesterol contained by shrimps and pork. Third, meat was coated with thin gruel and quickly roasted and cleared of the dried gruel membrane, which prevented nutrients from exuding and helped to make the meat well-done. Fourth, The fruit of paper mulberry trees has the protease which can soften meat. Therefore when meat was boiled with th fruit of paper mulberry trees, it can be softened easily. Fifth, pork was smoked over the weak fire of thatch. Sixth, in cooking dog meat, distilled dry steaming raised the boiling point and made it possible to preserve meat longer. Seventh, in boiling the sole of a bear, lime was added, which made meat tender by making the pH lower or higher than that of raw meat. Finally, in boiling down rice gluten, a porcelain in the pot prevented boiling over the brim, which is applied to pots in which to boil medical herbs.

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개에서 분리된 $Malassezia$ $pachydermatis$의 특성과 Essential Oil의 항진균 효과 (Characteristics of $Malassezia$ $pachydermatis$ Isolated from Dogs and Antifungal Effect of Essential Oils)

  • 김주연;;손원근
    • 한국임상수의학회지
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    • 제29권2호
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    • pp.141-147
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    • 2012
  • 이 연구는 개의 귀 속에서 분리한 $Malassezia$ $pachydermatis$의 특성과 이들 효모에 대한 essential oil들의 항진균효과를 측정한 결과로서 일반적인 $M.$ $pachydermatis$를 분리하기위한 Sabouraud dextrose agar (SDA), olive oil이 첨가된 SDA (SDAO) 배지 및 지방 친화성 효모를 검출하기 위한 Leeming's medium (LM)으로 구성된 세종류의 분리배지가 사용되었다. $Malassezia$ spp는 77.8%의 개로부터 분리되었으며 분리배지에 따라 분리율에 큰 차이를 나타내었으며 지방성분이 포함된 배지에서의 분리율이 일반적으로 사용하는 SDA에서 보다 높았다. 분리된 모든 $Malassezia$ spp는 $M.$ $pachydermatis$로 동정되었지만 육안적으로 보이는 집락의 모양과 SDA에서의 증식속도를 고려했을 때 4개의 아종으로 관찰되었다. 배양 72시간 후 집락의 직경이 3 mm 이상인 큰 집락(LC)과 2 mm 정도인 중간 집락(MC) 및 배양 5일 후에도 1 mm 이상으로 크지지 않는 작은 집락(SC), 그리고 SDA에서 증식하지 않는 지방의존 집락(Lipo)으로 분류되었다. SDA, SDAO 및 LM 배지에서 각 type의 효모가 분리된 정도를 보면, LC type 효모는 각각 4, 11, 11개 시료에서, MC type 효모는 각각 2, 3, 1개 시료에서, SC type 효모는 각각 1, 2, 1개시료에서 분리되었으며 Lipo type은 SDAO과 LM 배지 중 3 곳에서 분리되었다. $M.$ $pachydermatis$에 대한 essential oil의 항진균효과 측정은 디스크 확산법과 well 확산법에 의해 이루어졌으며, 대부분의 essential oil은 0.5에서 1.0%의 농도에서 $M.$ $pachydermatis$ 의 증식을 억제하였다. MIC90과 MIC50은 essential oil의 성분에 따라 다양하였으나, Thyme oil이 가장 억제 효과가 좋았으며 marjoram과 tea tree oil은 비교적 낮은 억제 능력을 나타내었다.