• Nuclear Engineering and Technology
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• 제48권3호
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• pp.597-607
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• 2016

Personalized medicine is tailored medical treatment that targets the individual characteristics of each patient. Theragnosis, combining diagnosis and therapy, plays an important role in selecting appropriate patients. Noninvasive in vivo imaging can trace small molecules, antibodies, peptides, nanoparticles, and cells in the body. Recently, imaging methods have been able to reveal molecular events in cells and tissues. Molecular imaging is useful not only for clinical studies but also for developing new drugs and new treatment modalities. Preclinical and early clinical molecular imaging shows biodistribution, pharmacokinetics, mechanisms of action, and efficacy. When therapeutic materials are labeled using radioisotopes, nuclear imaging with positron emission tomography or gamma camera can be used to treat diseases and monitor therapy simultaneously. Such nuclear medicine technology is defined as radiation theragnosis. We review the current development of drugs and technology for radiation theragnosis using peptides, albumin, nanoparticles, and cells.

• Bulletin of the Korean Chemical Society
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• 제35권8호
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• pp.2559-2562
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• 2014

• BMB Reports
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• 제49권6호
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• pp.303-304
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• 2016

Myoblast fusion is important for skeletal muscle formation. Even though the knowledge of myoblast fusion mechanism has accumulated over the years, the initial signal of fusion is yet to be elucidated. Our study reveals the novel function of a phosphatidylserine (PS) receptor, stabilin-2 (Stab2), in the modulation of myoblast fusion, through the recognition of PS exposed on myoblasts. During differentiation of myoblasts, Stab2 expression is higher than other PS receptors and is controlled by calcineurin/NFAT signaling on myoblasts. The forced expression of Stab2 results in an increase in myoblast fusion; genetic ablation of Stab2 in mice causes a reduction in muscle size, as a result of impaired myoblast fusion. After muscle injury, muscle regeneration is impaired in Stab2-deficient mice, resulting in small myofibers with fewer nuclei, which is due to reduction of fusion rather than defection of myoblast differentiation. The fusion-promoting role of Stab2 is dependent on its PS-binding motif, and the blocking of PS-Stab2 binding impairs cell-cell fusion on myoblasts. Given our previous finding that Stab2 recognizes PS exposed on apoptotic cells for sensing as an "eat-me" signal, we propose that PS-Stab2 binding is required for sensing of a "fuse-me" signal as the initial signal of myoblast fusion.

• Bulletin of the Korean Chemical Society
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• 제34권10호
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• pp.2861-2862
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• 2013

• BMB Reports
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• 제48권6호
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• pp.342-347
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• 2015

The expression of epidermal growth factor receptor (EGFR) is an important diagnostic marker for triple-negative breast cancer (TNBC) cells, which lack three hormonal receptors: estrogen and progesterone receptors as well as epidermal growth factor receptor 2. EGFR transactivation can cause drug resistance in many cancers including TNBC, but the mechanism underlying this phenomenon is poorly defined. Here, we demonstrate that insulin treatment induces EGFR activation by stimulating the interaction of EGFR with insulin-like growth factor receptor 1 (IGF-1R) in the MDA-MB-436 TNBC cell line. These cells express low levels of EGFR, while exhibiting high levels of IGF-1R expression and phosphorylation. Low-EGFRexpressing MDA-MB-436 cells show high sensitivity to insulinstimulated cell growth. Therefore, unexpectedly, insulin stimulation induced EGFR transactivation by regulating its interaction with IGF-1R in low-EGFR-expressing TNBC cells. [BMB Reports 2015; 48(6): 342-347]

• Nuclear Medicine and Molecular Imaging
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• 제42권5호
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• pp.337-346
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• 2008

Applications of nanotechnology in the medical field have provided the fundamentals of tremendous improvement in precise diagnosis and customized therapy. Recent advances in nanomedicine have led to establish a new concept of theragnosis, which utilizes nanomedicines as a therapeutic and diagnostic tool at the same time. The development of high affinity nanoparticles with large surface area and functional groups multiplies diagnostic and therapeutic capacities. Considering the specific conditions related to the disease of individual patient, customized therapy requires the identification of disease target at the cellular and molecular level for reducing side effects and enhancing therapeutic efficiency. Well-designed nanoparticles can minimize unnecessary exposure of cytotoxic drugs and maximize targeted localization of administrated drugs. This review will focus on major pharmaceutical nanomaterials and nanoparticles as key components of designing and surface engineering for targeted theragnostic drug development.

• Molecules and Cells
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• 제45권3호
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• pp.158-167
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• 2022

Ubiquitin (Ub) is post-translationally modified by Ub itself or Ub-like proteins, phosphorylation, and acetylation, among others, which elicits a variety of Ub topologies and cellular functions. However, N-terminal (Nt) modifications of Ub remain unknown, except the linear head-to-tail ubiquitylation via Nt-Met. Here, using the yeast Saccharomyces cerevisiae and an Nt-arginylated Ub-specific antibody, we found that the detectable level of Ub undergoes Nt-Met excision, Nt-deamination, and Nt-arginylation. The resulting Nt-arginylated Ub and its conjugated proteins are upregulated in the stationary-growth phase or by oxidative stress. We further proved the existence of Nt-arginylated Ub in vivo and identified Nt-arginylated Ub-protein conjugates using stable isotope labeling by amino acids in cell culture (SILAC)-based tandem mass spectrometry. In silico structural modeling of Nt-arginylated Ub predicted that Nt-Arg flexibly protrudes from the surface of the Ub, thereby most likely providing a docking site for the factors that recognize it. Collectively, these results reveal unprecedented Nt-arginylated Ub and the pathway by which it is produced, which greatly expands the known complexity of the Ub code.

• Investigative Magnetic Resonance Imaging
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• 제15권1호
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• pp.48-56
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• 2011

목적 : 본 연구에서는 금 나노 입자를 통한 국소 가열과 MR 온도 영상 기법을 결합한 Theragnosis 개념에 대한 가초연구로 금 나노 입자의 특성 및 제조 조건에 따른 MR 변수의 변화를 연구하였다. 대상 및 방법 : 실험실에서 제조된 구형과 막대형 금 나노 입자를 사용하였다. 구형 입자는 합성방법과 교반속도(stirring speed: rpm)를 변수로 설정하였고, 막대형 입자눈 첨가된 구형 입자의 양을 변수로 하여 조건을 다양화하였다. 금 나노 입자를 2% 아가로즈 젤에 1:1 로 혼합하여 임상용 1.5T MRI 시스템에서 신호를 획득하였고, $T_1$과 $T_2$ 이완시간의 측정을 위해 TR과 TE를 조절하였다. 획득한 영상의 화소별 신호 강도플 이용하여 제작한 소프트웨어로 $T_1$과 $T_2$ 이완곡선을 추정하였고, 통계 분석으로 유의성을 검증하였다. 결과 : 구형 입자의 평균 $T_1$ 값은 $1.86{\pm}0.04$초, 막대형은 평균 $2.08{\pm}0.04$초로 막대형이 더 걸게 측정되었고, 반면 평균 $T_2$ 값은 구형과 막대형 각각 $57{\pm}2.4$ ms와 $35.45{\pm}0.1$ ms로 구형 나노 입자에서 더 길게 측정되었다. 결론 : 금 나노 입자의 형태적 특성 및 제조 조건에 따른 MR 영상 변수 $T_1$과 $T_2$ 이완시간의 변화를 확인하였다. 금 나노 입자를 이용한 MR 영상 연구의 수행 시 금 나노 입자의 형태와 제조 조건에 따른 적절한 TR과 TE로 최적화된 영상을 얻을 수 있을 것으로 기대된다.

• Genomics & Informatics
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• 제14권1호
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• pp.12-19
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• 2016

Neuropeptides produced from prohormones by selective action of endopeptidases are vital signaling molecules, playing a critical role in a variety of physiological processes, such as addiction, depression, pain, and circadian rhythms. Neuropeptides bind to post-synaptic receptors and elicit cellular effects like classical neurotransmitters. While each neuropeptide could have its own biological function, mass spectrometry (MS) allows for the identification of the precise molecular forms of each peptide without a priori knowledge of the peptide identity and for the quantitation of neuropeptides in different conditions of the samples. MS-based neuropeptidomics approaches have been applied to various animal models and conditions to characterize and quantify novel neuropeptides, as well as known neuropeptides, advancing our understanding of nervous system function over the past decade. Here, we will present an overview of neuropeptides and MS-based neuropeptidomic strategies for the identification and quantitation of neuropeptides.

• 공업화학
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• 제33권1호
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• pp.113-118
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• 2022

Multifunctional nanomaterials based on mesoporous silica nanoparticles (MSN) and metal oxide nanocrystals are among the most promising materials for theragnosis because of their ease of modification and high biocompatibility. However, the preparation of multifunctional nanoparticles requires time-consuming multistep processes. Herein, we report a simple one-pot synthesis of multifunctional Mn₃O₄/mesoporous silica core/shell nanoparticles (Mn₃O₄@mSiO₂) involving the temporal separation of core formation and shell growth. This simple procedure greatly reduces the time and effort required to prepare multifunctional nanoparticles. Despite the simplicity of the process, the properties of nanoparticles are not markedly different from those of core/shell nanoparticles synthesized by a previously reported multistep process. The Mn₃O₄@mSiO₂ nanoparticles are biocompatible and have potential for use in optical imaging and magnetic resonance imaging.