• Journal of Microbiology and Biotechnology
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• v.18 no.11
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• pp.1773-1784
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• 2008

Effects of various industrially important carbon sources (glucose, sucrose, xylose, gluconate, and glycerol) on shikimic acid (SA) biosynthesis in Escherichia coli were investigated to gain new insight into the metabolic capability for overproducing SA. At the outset, constraints-based flux analysis using the genome-scale in silico model of E. coli was conducted to quantify the theoretical maximum SA yield. The corresponding flux distributions fueled by different carbon sources under investigation were compared with respect to theoretical yield and energy utilization, thereby identifying the indispensable pathways for achieving optimal SA production on each carbon source. Subsequently, a shikimate-kinase-deficient E. coli mutant was developed by blocking the aromatic amino acid pathway, and the production of SA on various carbon sources was experimentally examined during 51 batch culture. As a result, the highest production rate, 1.92 mmol SA/h, was obtained when glucose was utilized as a carbon source, whereas the efficient SA production from glycerol was obtained with the highest yield, 0.21 mol SA formed per mol carbon atom of carbon source consumed. The current strain can be further improved to satisfy the theoretically achievable SA production that was predicted by in silico analysis.

• Journal of Information Technology Services
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• v.17 no.4
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• pp.53-74
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• 2018

This study theoretically examines the essence of the competencies, qualifications and grades newly required in the era of the 4th industrial revolution, points out that the engineer rating system does not reflect the new talent and practical capability, and conducts theoretical and empirical analyzes on improvement of the rating system. The current rating system need to be improved because it is not possible for graduates and experience workers to upgrade. Also, it is reasonable that the current highest grade of engineer, the Professional Engineer, which is a sort of qualification, is not a grade but a function of calculating the grade. Based on this theoretical background, empirical analysis of the question investigation and focus group interview shows that the rating system should be improved to four grades or return to the previous system in 2013. And if it is reduced to four grade, it should go in the way that the professional engineer and the qualified engineer combined. In the industry, the future skills of engineers are as follows : qualification, ability to use emerging technology, problem solving, understanding and utilization of major, and data management, etc. These can be summarized as qualification, education (degree) and career. In particular, the engineering industry considers qualifications, degrees, and career experience, and career experience as the most important elements of the rating system. In this regard, it is necessary to introduce a method that accurately reflects the career experience in the improvement of the engineer rating system.

• Journal of Korea Society of Digital Industry and Information Management
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• v.18 no.4
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• pp.97-110
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• 2022

The purpose of this study is to analyze the level of public data convergence capabilities of administrative organizations and to explore important variables in data-based organizational capabilities. The theoretical background was summarized on public data and use activation, joint use, convergence, administrative organization, and convergence constraints. These contents were explained Public Data Act, the Electronic Government Act, and the Data-Based Administrative Act. The research model was set as the data-based organizational capabilities effect by a data-based administrative capability, public data operation capabilities, and public data operation constraints. It was also set whether there is a capabilities difference data-based on an organizational operation by the level of data convergence capabilities. This study analysis was conducted with hierarchical cluster analysis and multiple regression analysis. As the research result, First, hierarchical cluster analysis was classified into three groups. It was classified into a group that uses only public data and structured data, a group that uses public data on both structured and unstructured data, and a group that uses both public and private data. Second, the critical variables of data-based organizational operation capabilities were found in the data-based administrative planning and administrative technology, the supervisory organizations and technical systems by public data convergence, and the data sharing and market transaction constraints. Finally, the essential independent variables on data-based organizational competencies differ by group. This study contributed. As a theoretical implication, this research is updated on management information systems by explaining the Public Data Act, the Electronic Government Act, and the Data-Based Administrative Act. As a practical implication, the activity reinforcement of public data should be promoting the establishment of data standardization and search convenience and elimination of the lukewarm attitudes and Selfishness behavior for data sharing.

• Journal of Service Research and Studies
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• v.11 no.3
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• pp.31-42
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• 2021

This study is a study on theoretical considerations on industrial design data in the era of data economy. This study aims to illuminate the value of industrial design data, interpret the meaning of the transition from design management to the design data era, and highlight the importance of literacy to interpret data for designers. The scope of research approaches the R&D capability dimension of a company to explore the industrial value of design. It limits the scope of research from an industrial point of view rather than the humanistic basis and aesthetic value of design. As a result of the study, it was found that the value of industrial design data is not in ex post evaluation, but in customer-oriented market prediction, and that the design industry data-led strategy is important. The key point is that the industrial design data issues of large companies and SMEs are different, the direction of industrial design data should be to support customer-centered decision making in the product/service development of companies, and the core competency is the amount of data or tools and technologies to handle it. No, it should be in data literals. Lastly, if the use of industrial design data is to be strengthened, management of the public level rather than the personal level of data management should be preceded.

• Journal of Engineering Education Research
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• v.27 no.2
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• pp.35-50
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• 2024

The purpose of this study is to identify the core competencies of graduate students at A research university in the context of graduate education in science and engineering, and to develop and validate a diagnostic tool to measure them. To achieve the research objectives, first, 6 factors and 18 sub-competencies of core competencies were derived based on a review of domestic and foreign studies, cases of excellent research-centered overseas universities, and interviews with members of A University. Second, a theoretical model was constructed by deriving behavioral indicators based on the core competencies and sub-competencies, and a preliminary survey was conducted on 188 graduate students of University A to verify the statistical validity of the theoretical model. Results of exploratory and confirmatory factor analysis, the core competencies of graduate students at A research university consisted of 6 factors, 16 sub-competencies, and 77 items. Specifically, it included "Independent research capability(13 items)", "Social Entrepreneurship(10 items)", "Academic agility(15 items)", "Ingenious Challenges(15 items)", "Collegial Collaboration(9 items)", and "Mueunjae leadership(15 items)". This study contributes to the development of theories related to core competencies of graduate students in science and engineering, and has practical significance as a basis for a data-driven competency-based graduate education system.

• Journal of Distribution Research
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• v.15 no.5
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• pp.127-153
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• 2010

This study explores the locomotives of the growth in the Korean online shopping industry upon the theoretical basis based on the last 10 years' rapid changing environment. This attempt reveals the counter-arguments against preemtive effects based on the observation of reintermediation process in the online industry. We reviewed the NEBIC model proposed by Wheeler(2002) and propose the growth model, double helix framework based on the dynamic capability view. Furthermore the relevance of the proposed framework was validated with the review of last 10 years' sales and market share data in the online shopping industry. Meanwhile we found the limits of online market growth with the open market domination. So future of the online shopping retailers is depending on the development of the channel functions and merchandising on the basis of self-capability. Based on the tentative conclusion, we also suggest implications for the policy makers. Firstly policy facilitating the specialization of the power sellers incubased in the open market is necessary for the sustainable online market growth. And the establishment of the control tower is suggested to coordinate the consistency of the policies and regulations. And the device of the incentive is also proposed to strengthen the open markets' function to facilitate the small and medium online merchants.

• Advances in nano research
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• v.12 no.5
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• pp.501-514
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• 2022

This study aimed to investigate the effects and potential mechanisms of Chikusetsusaponin V (CsV) on endothelial nitric oxide synthase (eNOS) and vascular endothelial cell functions. Different concentrations of CsV were added to animal models, bovine aorta endothelial cells (BAECs) and human umbilical vein endothelial cells (HUVECs) cultured in vitro. qPCR, Western blotting (WB), and B ultrasound were performed to explore the effects of CsV on mouse endothelial cell functions, vascular stiffness and cellular eNOS mRNA, protein expression and NO release. Bioinformatics analysis, network pharmacology, molecular docking and protein mass spectrometry analysis were conducted to jointly predict the upstream transcription factors of eNOS. Furthermore, pulldown and ChIP and dual luciferase assays were employed for subsequent verification. At the presence or absence of CsV stimulation, either overexpression or knockdown of purine rich element binding protein A (PURA) was conducted, and PCR assay was employed to detect PURA and eNOS mRNA expressions, Western blot was used to detect PURA and eNOS protein expressions, cell NO release and serum NO levels. Tube formation experiment was conducted to detect the tube forming capability of HUVECs cells. The animal vasodilation function test detected the vasodilation functions. Ultrasonic detection was performed to determine the mouse aortic arch pulse wave velocity to identify aortic stiffness. CsV stimulus on bovine aortic cells revealed that CsV could upregulate eNOS protein levels in vascular endothelial cells in a concentration and time dependent manner. The expression levels of eNOS mRNA and phosphorylation sites Ser1177, Ser633 and Thr495 increased significantly after CsV stimulation. Meanwhile, CsV could also enhance the tube forming capability of HUVECs cells. Following the mice were gavaged using CsV, the eNOS protein level of mouse aortic endothelial cells was upregulated in a concentration- and time-dependent manner, and serum NO release and vasodilation ability were simultaneously elevated whereas arterial stiffness was alleviated. The pulldown, ChIP and dual luciferase assays demonstrated that PURA could bind to the eNOS promoter and facilitate the transcription of eNOS. Under the conditions of presence or absence of CsV stimulation, overexpression or knockdown of PURA indicated that the effect of CsV on vascular endothelial function and eNOS was weakened following PURA gene silence, whereas overexpression of PURA gene could enhance the effect of CsV upregulating eNOS expression. CsV could promote NO release from endothelial cells by upregulating the expression of PURA/eNOS pathway, improve endothelial cell functions, enhance vasodilation capability, and alleviate vessel stiffness. The present study plays a role in offering a theoretical basis for the development and application of CsV in vascular function improvement, and it also provides a more comprehensive understanding of the pharmacodynamics of CsV.

• Proceedings of the Technology Innovation Conference
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• 1996.12a
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• pp.27-62
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• 1996

As an exploratory attempt, this study examines the patterns of global R&D activities of industrial firms, focusing on the management of overseas R&D centers in terms of their location, primary missions, management style, organizational structure and communication and global human resource management system Based on a review of existing studies mainly undertaken in advanced countries and cases of foreign MNCs, several hypothetical relationships are postulated : 1) There appears a strong relationship between the purpose and location of R&D activities : 2) Different levels of global management and technological capability lead to different types of overseas R&D organizations : 3) The relationship between autonomy of overseas R&D organizations and their R&D performance will be moderated by organization size, development time pressure, and a need for internal efficiency : 4) Frequent communication between the headquarter and overseas R&D organizations will increase R&D performance 5) Mutual exchange of R&D professionals between the headquarter and overseas R&D organizations will be positively related with R&B performance. In addiction, this study explores the current state of global R&D activities of selective firms in Korea, based on ie-depth interviews, and analyzes the sources of problems in their global R&D management. Finally, theoretical and managerial implications of this study are discussed and future research directions are proposed.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.3 no.6
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• pp.575-596
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• 2009

Facing limited network resources such as bandwidth and processing capability, the Internet will have congestion from time to time. In this paper, we propose a scheme to maximize the total utility offered by the network to the end user during congested times. We believe the only way to achieve our goal is to make the scheme application-aware, that is, to take advantage of the characteristics of the application. To make our scheme scalable, it is designed to be class-based. Traffic from applications with similar characteristics is classified into the same class. We adopted the RED queue management mechanism to adaptively control the traffic belonging to the same class. To achieve the optimal utility, the traffic belonging to different classes should be controlled differently. By adjusting link bandwidth assignments of different classes, the scheme can achieve the goal and adapt to the changes of dynamical incoming traffic. We use the control theoretical approach to analyze our scheme. In this paper, we focus on optimizing the control on two types of traffic flows: TCP and Simple UDP (SUDP, modeling audio or video applications based on UDP). We derive the differential equations to model the dynamics of SUDP traffic flows and drive stability conditions for the system with both SUDP and TCP traffic flows. In our study, we also find analytical results on the TCP traffic stable point are not accurate, so we derived new formulas on the TCP traffic stable point. We verified the proposed scheme with extensive NS2 simulations.

• International Journal of Aeronautical and Space Sciences
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• v.15 no.4
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• pp.396-411
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• 2014

Synchronized switch damping (SSD) techniques have recently been developed for structural vibration control using piezoelectric materials. In these techniques, piezoelectric materials are bonded on the vibrating structure and shunted by a network of electrical elements. These piezoelectric materials are switched according to the amplitude of the excitation force to damp vibration. This paper presents a new SSD technique called 'synchronized switch damping on negative capacitance and adaptive voltage sources' (SSDNCAV). The technique combines the phenomenon of capacitance transient charging and electrical resonance to effectively dampen the structural vibration. Also, the problem of stability observed in the previous SSD techniques is effectively addressed by adapting the voltage on the piezoelectric patch according to the vibration amplitude of the structure. Analytical expressions of vibration attenuation at the resonance frequency are derived, and the effectiveness of this new technique is demonstrated, for the control of a resonant cantilever beam with bonded piezoelectric patches, by comparing with SSDI, SSDVenh, and SSDNC techniques. Theoretical predictions and experimental results show the remarkable vibration damping capability of SSDNCAV technique, which was better than the previous SSD techniques. The broadband vibration control capabilities of SSDNCAV technique are also demonstrated, which exceed those of previous SSD techniques.