• Journal of Biosystems Engineering
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• v.30 no.6 s.113
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• pp.340-346
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• 2005

Major quality features of the beef carcass in most countries including Korea are size, marbling state of the lean tissue, color of the fat and lean tissue, and thickness of back fat of the 13th rib. To evaluate the beef quality, extracting loin parts from the sectional image of the 13th beef rib is crucial and is the first step. However, because of the inhomogeneous distribution and fuzzy pattern of the fat and lean tissues on the beef cut, it is difficult to extract automatically the proper contour of the lean tissue. In this paper, a prototype mobile beef quality measurement system, which can be implemented practically at the beef processing site was developed. The developed system was composed of the hand held image acquisition unit and mobile processing unit mounted with touch-pad screen. Algorithms to extract the boundary of the lean tissue and a proper tool to evaluate the marbling status have been developed using color image processing. The boundary extraction algorithm showed successful results for the beef cuts with simple and moderate patterns of the lean tissue and fat. However, it had some difficulty in eliminating complex pattern of the extraneous tissues adhered to the lean tissue in the boundary extraction. The developed algorithms were implemented to the prototype mobile processing unit.

• Asian-Australasian Journal of Animal Sciences
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• v.12 no.3
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• pp.388-394
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• 1999

Two experiments were conducted to subdivide isoleucine (exp. 1) and valine (exp. 2) requirements for maintenance from the requirements for growth of broilers aged 1 to 3 weeks. Purified diets were used, containing five graded levels of isoleucine and valine. Based on weight gain response, the isoleucine requirement for growth was 7.50 mg/g weight gain and the daily isoleucine need for maintenance (mg) was 0.044 per unit metabolic body size ($(Wg^{0.75})$). Based on the N gain response, the isoleucine requirement for growth was 0.317 mg/mg N gain and the daily isoleucine need for maintenance (mg) was 0.040 per unit metabolic body size $(Wg^{0.75})$. Based on weight gain and N gain response, the total isoleucine requirement was calculated 244 mg/day or 0.59% of the diet, 274 mg/day or 0.66% of the diet, respectively. From the relationship of weight gain and N gain, 5.07% of the retained protein was comprised of isoleucine; the reported isoleucine content of chick muscle was 4.42%. The valine requirement for growth was 9.84 mg/g weight gain and 0.36 mg/mg N gain whereas the maintenance requirement was 0.046 or 0.052 mg per unit of metabolic body size (Wgo.11. According to the model developed to estimate valine requirement, the total requirement was 319 mg/day or 0.77% of the diet, 315 mg/day or 0.76% of the diet, respectively. Previous reported valine requirements for growing chicks of 7~24 days old were in close agreement with these estimates. As a percentage of retained protein, valine was calculated to be 5.81% ; the reported valine concentration of crude protein of chicks' body including feathers was 6.72%.

• Journal of the Korean Operations Research and Management Science Society
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• v.19 no.1
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• pp.145-152
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• 1994

This paper deals with an economic design of acelerated life test under constant stresses where failure times are exponentially distributed. In this case the optimization criterion is the information amount per unit cost. Fisher's information matrix of exponential distribution's parameters and expected cost considering fixed and variable costs are obtained. The decision variable is the censoring time in the model. In the 2-level constant stress case, it is proved that the optimal solution exists and is unique under some condition. Numerical examples are also included.

• Korean Journal of Microbiology
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• v.39 no.4
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• pp.223-229
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• 2003

The purpose of this work was to perform the characterization of NAD(P)H-nitroreductase isolated from Stenotrophomonas sp. OK-5 capable of degrading 2,4,6-trinitrotoluene (TNT). Initially, NADP(H)-nitroreductase by a series of purification processes including ammonium sulfate precipitation, DEAE-sepharose, andQ-sepharose was prepared. From samples harvested from fraction collector, three different fractions (I, II & III)having the enzyme activity of NAD(P)H-itroreductase were detected. Specific activities of three fractions I, II,and III of NAD(P)H-nitroreductase were determined to approximately 5.06 unit/mg, 4.95 unit/mg and 4.86 unit/mg, and concentrated to 10.5, 9.8, and 8.9-fold compared to crude extract, respectively. Among these three fractions,the fraction I of NAD(P)H-nitroreductase demonstrated the highest specific activity in this experiment. Several factors affecting on the enzyme activity of NAD(P)H-nitroreductase (fractions I, II & III) were investigated.The optimum temperature of all NAD(P)H-nitroreductase (fractions I, II & III) was 30oC, and the optimal pH was approximately 7.5. Metal ions such as Ag+, Cu2+, Hg2+ inhibited approximately 80% enzyme activity of all NAD(P)H-nitroreductase, and the enzyme activities were decreased about 30-40% inhibition in the presence of Mn2+ or Ca2+. However, Fe3+ showed stimulatory effect on the enzyme activity. The molecular weights of NAD(P)H-nitroreductase (fractions I, II & III) were measured about 27 kDa on the SDS-PAGE.

• Proceedings of the Korean Society of Life Science Conference
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• 2002.03a
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• pp.98-98
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• 2002

• Journal of Life Science
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• v.22 no.6
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• pp.837-843
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• 2012

This study was carried out to investigate the morphological and physiological characteristics of six new cultivars of Hypsizygus marmoreus (Hm) and measure endo-, exo-cellular enzyme-specific activity. The domestic wild stain (Hm3-10) and commercial strain in Japan (Hm1-1) were mated by crossing monokaryon mycelia. We gained 58 strains from one of 400 crosses through the $1^{st}$ cultivation experiment, and selected six strains from one of 58 strains through the $2^{nd}$ cultivation experiment. When six of the selected new strains were grown during several spawn culture periods (60, 70, 80, 90, and 100 days), a spawn culture period of more 80 days was considered to be excellent as being shorter than 19~20 days. Therefore, we determined the period of spawn culture as 80 days. Three strains such as Hm15-3, Hm15-4, and Hm17-5 showed an excellent result. When endo-cellular enzyme activity measured eight strains, we obtained a result of that specific activity of ${\alpha}$-amylase at the highest as 73.9~102.2 unit/mg protein, and chitinase is lower than ${\alpha}$-amylase at 8.1~13.1 unit/mg protein. When exo-cellular enzyme activity measured eight strains, we determined the result of that specific activity of ${\alpha}$-amylase is the highest at 5,292~1,184 unit/mg protein, and CMCase and xylanase were 1,140~245 unit/mg protein, 94~575 unit/mg protein, compared to each other. However, the enzyme activity of ${\beta}$-glucosidase and chitinase is low.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.122-133
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• 1997

Purified diets containing 5 graded levels of threonine were fed to young, growing and finishing pigs to determine the threonine requirement for growth and maintenance. A model was developed to subdivide the threonine requirement for the maintenance from the requirement for growth. From this model, the threonine requirement for growth was 7.733, 10.968 and 11.235 g/kg live weight gain and the maintenance requirement was 0.118, 0.048 and 0.024 g per unit of metabolic body size at each stage of growth, respectively. In the young pigs, the threonine requirement for growth was 0.388 g/g N gain and the maintenance requirement was 0.122 g per unit of metabolic body size. The breakpoint of plasma threonine concentrations was 3.995, 7.933 and 7.738 g/d, respectively. Expected requirements obtained from these formulae were in general agreement with previous estimates. Based on the weight gain vs N gain equation, about 4.24% of the retained protein was comprised of threonine and compared to 3.81%, the mean threonine content of pig muscle CP.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.86-97
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• 1997

Purified diets containing 5 graded levels of methionine + cystine were fed to young, growing and finishing pigs to determine the methionine + cystine requirement for growth and maintenance. A model was developed to subdivide the methionine + cystine requirement for maintenance from requirement for growth. From this model, the methionine + cystine requirement for growth was 8.633, 10.260 and 9.293 g/kg live weight gain and the maintenance requirement was 0.049, 0.016 and 0.019 g per unit of metabolic body size at each stage of growth, respectively. In the young pigs, the methionine + cystine requirement for growth was 0.491 g/g N gain and the maintenance requirement was 0.059 g per unit of metabolic body size. The breakpoint of plasma methionine + cystine concentrations was 3.888, 6.935 and 8.116 g/d, respectively. Expected requirements obtained from these formulae were in general agreement with previous estimates. Based on the weight gain vs N gain equation, about 4.44% of the retained protein was comprised of methionine + cystine and compared to 3.31%, the mean methionine + cystine content of pig muscle CP.

• Asian-Australasian Journal of Animal Sciences
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• v.10 no.1
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• pp.54-63
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• 1997

Purified diets containing 5 graded levels of lysine were fed to young and growing pigs to determine the lysine requirement for growth and maintenance. A model was developed to subdivide the lysine requirement for the maintenance from requirement for growth. From this model, the lysine requirement for growth was 18.018 and 19.431 g/kg live weight gain and the maintenance requirement was 0.115 and 0.033 g per unit of metabolic body size at each stage of growth, respectively. In the young pigs, the lysine requirement for growth was 0.950 g/g N gain and the maintenance requirement was 0.114 g per unit of metabolic body size. The breakpoint of plasma lysine concentrations was 8.695 and 13.464 g/d, respectively. Expected requirements obtained from these formulae were in general agreement with previous estimates. Based on weight gain vs N gain equation, about 7.92% of the retained protein was comprised of lysine as compared to 7.11%, the mean lysine content of pig muscle CP.

• Journal of the Korean Society of Earth Science Education
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• v.1 no.1
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• pp.85-98
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• 2008

Though the investigation of the suggested references proverbs related weather were collected and considered the probability of the usage on science instruction. The results are as follows. 130 proverbs related to weather were collected through the investigation of the suggested references. Most of weather proverbs are based on the states of sky, clouds, wind, precipitation, actions of animal, states of plants and the condition of people in daily life. they were classified according to weather types, natural phenomena and seasons. According to the results of analysing the students' recognition and interests on weather proverbs, most of students heard weather proverbs in their elementary school years firstly through their parents, books, and teachers. However they have only heard a few. Also many students also tend to think weather proverbs have a scientific base because weather proverbs have been told by many people through the ancient time and correspond to their personal experiences. Students responded that weather proverbs are useful for science learning on weather and can teach heritage and wisdom. After reading the suggested weather proverbs, their interests on weather proverbs were increased more than before reading. This is one of reasons why weather proverbs can be introduced to science classes. Weather proverbs were considered related to science curriculum. The third grade has a Unit "Fine days and Cloudy days", the fifth grade, "Unit of Weather Change", the Sixth grade, "Unit of Weather Forecast" , the Ninth grade, "Unit of Water cycle and Weather Change" and the tenth grade has "Unit of Earth". So the author consider that weather proverb materials can be used so effectively to bring about interest and motive in science learning.