• Journal of agriculture & life science
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• v.45 no.3
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• pp.97-104
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• 2011

Analyses of export of agricultural products, so far, have not been an easy task because of the lack of direct data from exporting companies. Results obtained from indirect data are subject to criticism and have a restriction on general interpretation. This paper uses and analyzes the direct data from a chrysanthemum-exporting-company, leading to some conclusion that farms receive on average 72.3% of unit price but the share decreases by the distance to the company. Exporting farms have higher income ratio, compared to farms that sell flowers to domestic market. The analysis of unit price and cost shows that the profitability of exporting-company might be worsened if government subsidy is not in place.

• Journal of Life Science
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• v.10 no.2
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• pp.125-130
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• 2000

To overproduce endoxylanase from a recombinant Bacillus subtilis harboring the pJHKJ4 plasmid, the effects of carbon and nitrogen sources on the cell growth and expression level of endoxylanase were investigated in the flask cultures. Among the various carbon and nitrogen sources tested, glucose and maltose as carbon source and yeast extract as nitrogen source were found to be the most effective for the cell growth and the endoxylanase expression. When the concentration of glucose was increased from 0.5% to 5%, the highest activity of extracellular endoxylanse, 166 unit/$m\ell$, was observed at 2% glucose. In case of maltose, the endoxylanase was stably produced at the level of 180 unit/$m\ell$, regardless of the concentration of maltose. The higher the concentration of yeast extract, the greater cell growth and endoxylanase expression were obtained. However, the highest endoxylanase activity per unit cell mass was observed with 1% yeast extract. With the optimized medium (2% glucose, 1% yeast extract, etc), about 630 unit/$m\ell$ of endoxylanse was expressed through the batch fermentation in a fermentor, which expression level corresponded to about 0.7 g-endoxylanase protein /$\ell$. It was also found that the plasmid was stably maintained above 70% level, and more than 90% of endoxylanase activity was detected in the extracellular medium.

• Journal of Environmental Science International
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• v.22 no.3
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• pp.269-279
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• 2013

In this paper, an environmental assessment was carried out on the whole process of industrial business activities to establish a basic plan for climate change mitigation and energy independency. The whole process was divided into each discharge process in terms of water, air, solid waste, green house gases and refractory organic compounds. The flowcharts and basic unit of process were analysed for three years (2008-2010), being utilized as basic information for the life cycle assessment. It was found that the unit loading for the whole process significantly depends on changes in the operation rate change and highly concentrated wastewater inflow. About 35% of solid waste production was reduced by improving the incineration method with co-combustion in coal boiler, generating about 57% of electricity used for the whole process, and consequently reducing the energy costs. As the eco-efficiency index was found to be more than 1, compared to the previous years, it can be said that improvement in general has taken place.

• Journal of Life Science
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• v.14 no.4
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• pp.614-619
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• 2004

This study was undertaken to investigate the different responses of cultured plant cells to paraquat treatment and nucleus-DNA damage in relation to enzyme activity of superoxide dismutase (SOD). Furthermore, this study was also carried out to understand the antioxidative mechanism of plant cells to environmental stress. We selected two different species of plant cultured cells, Ipomoea batatas as high-SOD species and Lonicera japonica as low-SOD species. The total activity and specific activity of SOD in a chlorophyllous cell of I. batatas were 3,736 unit/gㆍfresh weight and 547 unit/mgㆍprotein, respectively, and those in L. japonica were 23 unit/gㆍfresh weight and 13 unit/mgㆍprotein, respectively SOD activity in chlorophyllous I. batatas cells reached its maximum level at 10 to 15 days after subculture, whereas that in L. japonica remained at a very low SOD level during the whole period of subculture. In comparison to L. japonica, I. batatas, a high-SOD species, showed high tolerance to paraquat 10 and 50 mg/l treatment in terms of cell viability and electrolyte leakage. Based on the result of comet assay, the nucleus-DNA damage of two species by paraquat 50 mg/l treatment was not significantly different. However, I. batatas cells repaired their damaged DNA more effectively than the cells of the low-SOD species, L. japonica.

• Journal of Microbiology and Biotechnology
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• v.27 no.8
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• pp.1472-1482
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• 2017

Bacterial cytochrome P450 (CYP) steroid hydroxylases are effectively useful in the pharmaceutical industry for introducing hydroxyl groups to a wide range of steroids. We found a putative CYP steroid hydroxylase (BaCYP106A2) from the bacterium Bacillus sp. PAMC 23377 isolated from Kara Sea of the Arctic Ocean, showing 94% sequence similarity with BmCYP106A2 (Bacillus megaterium ATCC 13368). In this study, soluble BaCYP106A2 was overexpressed to evaluate its substrate-binding activity. The substrate affinity ($K_d$ value) to 4-androstenedione was $387{\pm}37{\mu}M$. Moreover, the crystal structure of BaCYP106A2 was determined at $2.7{\AA}$ resolution. Structural analysis suggested that the ${\alpha}8-{\alpha}9$ loop region of BaCYP106A2 is intrinsically mobile and might be important for initial ligand binding. The hydroxyl activity of BaCYP106A2 was identified using in vitro enzyme assays. Its activity was confirmed with two kinds of steroid substrates, 4-androstenedione and nandrolone, using chromatography and mass spectrometry methods. The main products were mono-hydroxylated compounds with high conversion yields. This is the second study on the structure of CYP106A steroid hydroxylases, and should contribute new insight into the interactions of bacterial CYP106A with steroid substrates, providing baseline data for studying the CYP106A steroid hydroxylase from the structural and enzymatic perspectives.

• Journal of the Korean Society of Safety
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• v.29 no.1
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• pp.7-14
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• 2014

Inverter module, which feeds the converted power to the traction motor for EMU. Consists of the power semiconductors with their gate drive unit(GDU)s and the control computer for driving, voltage, current and speed controls. Electrolytic condenser, connected to the gate drive unit and a core component to drive the power semiconductor, has problems such as reduction in lifetime and malfunction caused by electrical and mechanical characteristic changes from heat generation during high speed switching for generation of stable power. In this study, To check the service life of electrolytic condenser, the test was carried out in two ways. First, In the case of accelerated life testing of condenser, the Arrhenius model is a way of life testing. Another way is to analyze the reliability of the failure data by the method of parametric data analysis. Eventually, life time by accelerated life test than a method of failure data analysis(Weibull distribution) was found to be slightly larger output.

• Journal of Microbiology and Biotechnology
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• v.33 no.3
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• pp.387-397
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• 2023

Cytochrome P450 (CYP) is a heme-containing enzyme that catalyzes hydroxylation reactions with various substrate molecules. Steroid hydroxylases are particularly useful for effectively introducing hydroxyl groups into a wide range of steroids in the pharmaceutical industry. This study reports a newly identified CYP steroid hydroxylase (BaCYP106A6) from the bacterium Bacillus sp. and characterizes it using an in vitro enzyme assay and structural investigation. Bioconversion assays indicated that BaCYP106A1 catalyzes the hydroxylation of progesterone and androstenedione, whereas no or low conversion was observed with 11β-hydroxysteroids such as cortisol, corticosterone, dexamethasone, and prednisolone. In addition, the crystal structure of BaCYP106A6 was determined at a resolution of 2.8 Å to investigate the configuration of the substrate-binding site and understand substrate preference. This structural characterization and comparison with other bacterial steroid hydroxylase CYPs allowed us to identify a unique Arg295 residue that may serve as the key residue for substrate specificity and regioselectivity in BaCYP106A6. This observation provides valuable background for further protein engineering to design commercially useful CYP steroid hydroxylases with different substrate specificities.

• Korean Security Journal
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• no.41
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• pp.241-261
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• 2014

The main objective of this research was to grasp using a structure equation analysis if an auxiliary police officer unit's measure of getting rid of assault gets to their adaptation to the unit through stress factor to reveal the cause of an auxiliary police officer unit's adaptation to unit. The data used for the research is survey data of all auxiliary police officer belonging to Ulsan Metropolitan Police Agency in 2011. The result of structure equation analysis showed that the mediated effect through stress was statistically meaningful in the level of p<.001. Also, the result of confirming the size of effect through standardized regression coefficients was that the effect of stress upon the adjustment to units was higher than the effect of unit's measure of getting rid of assault. Namely, unit's measure of getting rid of assault has a positive effect upon a conscripted policemen unit's adaptation to unit, but it showed that an auxiliary police officer unit's adaptation to unit had the bigger individual stress factor than that of unit's measure of getting rid of assault. From this, authorities should recognize that an auxiliary police officer's stress is a cause to determine their adaptation to units, and prepare its alternative.

• Journal of Microbiology and Biotechnology
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• v.18 no.1
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• pp.55-58
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• 2008

Alanine racemase, a bacterial enzyme belonging to the fold-type III group of pyridoxal 5'-phosphate (PLP)-dependent enzymes, has been shown to catalyze the interconversion between L- and D-alanine. The alanine racemase from the pathogenic bacterium Enterococcus faecalis v583 has been overexpressed in E. coli and was shown to crystallize an enzyme at 295 K, using polyethylene glycol (PEG) 8000 as a precipitant. X-ray diffraction data to $2.5{\AA}$ has been collected using synchrotron radiation. The crystal is a member of the orthorhombic space group, $C222_1$ with unit cell parameter of a=94.634, b=156.516, $c=147.878{\AA},\;and\;{\alpha}={\beta}={\gamma}=90{\AA}$. Two or three monomers are likely to be present in the asymmetric unit, with a corresponding $V_m\; of\;3.38{\AA}^3\;Da^{-1}\;and\;2.26{\AA}^3\;Da^{-1}$ and a solvent content of 63.7% and 45.5%, respectively.

• Journal of Korean Society of Rural Planning
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• v.20 no.2
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• pp.1-10
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• 2014

Smartphones change the picture of data and information sharing and make it possible to share various real-time flooding data and information. The vulnerability indicators of farmland inundation is needed to calculate the risk of farmland flood based on changeable hydro-meteorological data over time with morphologic characteristics of flood-damaged areas. To find related variables show the vulnerability of farmland inundation using the binary-logit model and correlation analysis and to provide vulnerability indicators were estimated by fuzzy set method. The outputs of vulnerability indicators were compared with the results of Monte Carlo simulation (MCS) for verification. From the result vulnerability indicators are applicable to mobile_based information system of farmland inundation.