• Journal of Ginseng Research
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• v.46 no.3
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• pp.357-366
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• 2022

Background: Withania somnifera (Solanaceae), generally known as Indian ginseng, is a medicinal plant that is used in Ayurvedic practice for promoting health and longevity. This study aims to identify the bioactive metabolites from Indian ginseng and elucidate their structures. Methods: Withanolides were purified by chromatographic techniques, including HPLC coupled with LC/MS. Chemical structures of isolated withanolides were clarified by analyzing the spectroscopic data from 1D and 2D NMR, and HR-ESIMS experiment. Absolute configurations of the withanolides were established by the application of NMR chemical shifts and ECD calculations. Anti-adipogenic activities of isolates were evaluated using 3T3-L1 preadipocytes with Oil Red O staining and quantitative real-time PCR (qPCR). Results: Phytochemical examination of the roots of Indian ginseng afforded to the isolation of six withanolides (1-6), including three novel withanolides, withasilolides GeI (1-3). All the six compounds inhibited adipogenesis and suppressed the enlargement of lipid droplets, compared to those of the control. Additionally, the mRNA expression levels of Fabp4 and Adipsin, the adipocyte markers decreased noticeably following treatment with 25 µM of 1-6. The active compounds (1-6) also promoted lipid metabolism by upregulating the expression of the lipolytic genes HSL and ATGL and downregulating the expression of the lipogenic gene SREBP1. Conclusion: The results of our experimental studies suggest that the withasilolides identified herein have anti-adipogenic potential and can be considered for the development of therapeutic strategies against adipogenesis in obesity. Our study also provides a mechanistic rationale for using Indian ginseng as a potential therapeutic agent against obesity and related metabolic diseases.

• The Korean Journal of Physiology and Pharmacology
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• v.6 no.2
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• pp.109-112
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• 2002

The signal pathways involved in the regulation of AP-1 DNA binding activity in long-term nicotine stimulated bovine adrenal medullary chromaffin (BAMC) cells have not been well characterized. To understand the involvement of second messengers in the regulation of AP-1 DNA binding activity, the present study was designed to define the time-course for inhibition of nicotine-induced responses by cholinergic antagonists, $Ca^{2+}$ and calmodulin (CaM) antagonists, and calcium/calmodulin-dependent protein kinase (CaMK) II inhibitor using electrophoretic mobility shift assay. Nicotine $(10{\mu}M)$ stimulation increased AP-1 DNA binding activity at 24 hr after treatment. Posttreatment with hexamethonium (1 mM) plus atropine $(1{\mu}M)$ (HA), nimodipine $(1{\mu}M),$ or calmidazolium $(1{\mu}M)$ at 0.5, 3, and 6 hr after the nicotine treatment significantly inhibited the AP-1 DNA binding activity increased by long-term nicotine stimulation. However, posttreatment with HA, nimodipine, or calmidazolium at 9 or 12 hr after the nicotine treatment did not affect the nicotine-induced increase of AP-1 DNA binding activity. The pretreatment of BAMC cells with various concentrations of KN-62 inhibited the increase of AP-1 DNA binding activity induced by nicotine in a concentration-dependent manner. KN-62 $(10{\mu}M)$ posttreatment beginning at 0.5, 3, or 6 hr after the nicotine treatment significantly inhibited the increase of AP-1 DNA binding activity. However, KN-62 posttreatment beginning at 9 or 12 hr after the nicotine treatment did not affect the increase of AP-1 DNA binding activity. This study suggested that stimulation (for at least 6 hr) of nicotinic receptors on BAMC cells was necessary for increase of AP-1 DNA binding activity, and activation of $Ca^{2+},$ CaM, and CaMK II up to 6 hr at least seemed to be required for the increase of nicotine-induced AP-1 DNA binding activity.

• Research in Community and Public Health Nursing
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• v.9 no.1
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• pp.213-222
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• 1998

The prevalence rate of low back pain in nurses is higher than that of other jobs, because there are many more risk factors(for example, standing postures, lifting and carrying heavy loads, transfering patients, changing a patient's position etc.). This investigation is to provide basic data for prevention of low back pain by analyzing the prevalence rate and related factors of low back pain in nurses(esp. operating room staff) in general hospitals. A self-reported questionaire survey was carried out on three hundred and five nurses of three general hospitals in Seoul from June, 1996 to August, 1996. Subjects of the survey were divided into a low back pain group(LBP) and a cotrol group to investigate the association between low back pain and the general characteristics and work related factors of each group. The results were as follows: 1. In the subjects of the survey, the prevalence rate of low back pain was 60.0% for the last year. 2. 73.6% of operating room(OR) staff complained of low back pain. 3. Standing for a long time and working in twisting postures were associated with low back pain, and so were the shift, the type of work, and the frequency of transfering patients. 4. The weight loads which nurses frequently carried were, under 5kg, 5-10kg, 15-20kg, 15-20kg, over 20kg, and all, except for 10-15kg, were associated with LBP. 5. 76.9% of the LBP experienced low back pain within 3 years after starting nursing jobs. 6. Concerning the degrees of low back pain: 75.8% was limited to waist; 18.8% reached the knees; 4.2% reached ankles. In conclusion, the prevalence rate of low back pain in general hospital nurses was associated with the works in operating room and other work related factors, therefore it is necessary to develop on educational program for the prevention of low back pain as well as and to improve working environments.

• International Journal of Oral Biology
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• v.35 no.2
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• pp.51-60
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• 2010

Few studies have evaluated the apoptosis-inducing efficacy of NaF on cancer cells in vitro but there has been no previous investigation of the apoptotic effects of NaF on human oral squamous cell carcinoma cells. In this study, we have investigated the mechanisms underlying the apoptotic response to NaF treatment in the YD9 human squamous cell carcinoma cell line. The viability of YD9 cells and their growth inhibition were assessed by MTT and clonogenic assays, respectively. Hoechst staining, DNA electrophoresis and TUNEL staining were conducted to detect apoptosis. YD9 cells were treated with NaF, and western blotting, immunocytochemistry, confocal microscopy, FACScan flow cytometry, and MMP and proteasome activity assays were performed sequentially. The NaF treatment resulted in a time- and dose-dependent decrease in YD9 cell viability, a dose-dependent inhibition of cell growth, and the induction of apoptotic cell death. The apoptotic response of these cells was manifested by nuclear condensation, DNA fragmentation, the reduction of MMP and proteasome activity, a decreased DNA content, the release of cytochrome c into the cytosol, the translocation of AIF and DFF40 (CAD) into the nucleus, a significant shift of the Bax/Bcl-2 ratio, and the activation of caspase-9, caspase-3, PARP, Lamin A/C and DFF45 (ICAD). Furthermore, NaF treatment resulted in the downregulation of G1 cell cyclerelated proteins, and upregulation of p53 and the Cdk inhibitor $p27^{KIP1}$. Taken collectively, our present findings demonstrate that NaF strongly inhibits YD9 cell proliferation by modulating the expression of G1 cell cycle-related proteins and inducing apoptosis via mitochondrial and caspase pathways.

• Journal of the Mineralogical Society of Korea
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• v.13 no.4
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• pp.171-185
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• 2000

Siliceous mudstones are embedded on a large scale in the Tertiary formations of Pohang area. Some useful zeolites such as NsP, (Na, TMA)P, analcime and hydroxysodalite were synthesized from the siliceous mudstones by treating with the variety of solution, i.e ., NaOH, NaOH+NaCl, NaOH, NaOH+$NaAlO_2$and NaOH+TMAOH at the low-temperature hydrothermal system ranging 60~12$0^{\circ}C$. Major precursor of zeolites is found as opal-CT in the zeolite-forming reaction. Smectite, which is included in considerable amounts in the mudstone, appears to play a major role of Al-source in the zeolite synthesis. In comparison, chalcedonic quartz and mica are rather insoluble in alkaline solution, and thus, these are observed as major impurities in the reaction products. An addition of $NaAlO_2$to NaOH solution is effective for eliminating these impurities in the reaction procedure, through these are partly dissolved when elevating the reaction temperature, concentration, and time. Phase change from NaP to hydroxysodalite takes place at the NaOH concentrations of 3.0~4.0 M, and the reaction is not sensitive to the temperature shift. NaP is more stable at lower NaOH concentration and higher activity of $Na_{+}$ whereas analcime is sensitive to the temperature change and stable at higher than $100^{\circ}C$ and 2.0~4.0 M in NaOH concentration. For the pure NaP synthesis without any other products, the treatment of mudstones with 1:1 solution of NaOH and $NaAlO _2$ turns out to be quite effective. NaP was successfully synthesized together with analcime at $100^{\circ}C$ as well as lower concentrations of NaOH+NaCl solution. In addition, the organic type, (Na, TMA)P was formed together with smectite in the 1:1 solution of NaOH and TMAOH.

• Korean Journal of Applied Biomechanics
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• v.28 no.1
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• pp.9-18
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• 2018

Objective: This study aimed to analyze how the upright body type exercise program affected body balance and record of archers. This study aimed to prove the effectiveness of upright body type exercise, on this basis, in enhancing the performance of archery players. Method: A total of 14 archers (7 men and 7 women) in B Metropolitan City who had ${\geq}4years$ of career in archery and were given explanation of its contents and purpose before giving spontaneous consent to the experiment were enrolled in the study. The upright body type exercise program was implemented thrice a week for 12 weeks, with higher exercise intensity with time. A resistive pressure sensor, Gaitview AFA-50, was used to measure the foot plantar pressure distribution and analyze quantitative information concerning variation in posture stability and weight shift in dynamic balance of foot plantar pressure in shooting and static balance of plantar pressure with the eyes open and closed and the change in archery record accompanying the change in body balance. Results: As for the differences in foot plantar pressure between before and after participation in the upright body type exercise program, there was no significant difference in static balance of foot plantar pressure with the eyes open, and there was statistically significant difference at the ${\alpha}=.05$ significance level in static balance of foot plantar pressure with the eyes closed or in dynamic balance of foot plantar pressure in shooting. There was statistically significant difference at the ${\alpha}=.05$ significance level in archery record. Conclusion: The upright body type exercise program had positive effects on static and dynamic balance of foot plantar pressure by allowing archers to experience less body sway and physical imbalance in shooting with closed eyes and positive effects on archery record. Thus, the program is expected to help archers correct their posture and perform better.

• Journal of the Korean Crystal Growth and Crystal Technology
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• v.31 no.5
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• pp.212-217
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• 2021

The applicability of the white LED from the blue LED of the coating film as a binder for surface and curved coatings were confirmed. The particle size of YAG is D₅₀: 9~10 ㎛, and the crystal structure is garnet (Y₃Al₅O₁₂), cubic. The coating film had no cracks, at the same time, the silica sol was uniformly coated with YAG phosphor, and the YAG content and thickness in the coating film showed a tendency to increase up to 40 ㎛ in proportion to the increase in the amount added. Furthermore, as the YAG content increased, the PL emission intensity increased and the color coordinate shift toward the end of the chromatic locus curve was confirmed.

• Rubber Technology
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• v.22 no.2
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• pp.119-149
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• 2021

Domestic materials & components industry is making a shift from quantitative growth to qualitative growth, while the paradigm of a future society is changing rapidly to energy, mobility, smartification and etc. The main features of materials & components industry are analyzed broadly based on three aspects that are: ① industry Status, ② the value chain structure, ③ the competitiveness. With regard to the national aspect, it is shown that the number of an export and a trade surplus increased consistently after the government advanced the policy on materials & components and Korea's trade deficit with Japan has been decreased. Moreover, the state's export ranking increased from tenth place in 2001 into sixth place in 2017, dedicating to the growth of a national economy. The analysis of the chain value indicates that specifications of an end product are set when the materials & components are combined with manpower, infrastructure and technologies. In terms of the competitiveness, it requires a long time and high costs to develop technologies on materials and it is important for components to have a process innovation and credibility wired. Following the industry structure with priority given on materials, Jeonnam Province accounts for 14.8% of national material production. As a result of analyzing Jeonnam's export competitiveness, it is measured that a serious polarization exists, revealing there are 6 major technology industries by 11 categories for materials & components, Jeonnam has presented 6 major materials & components considering the key industry and the new industry in future to remedy the mentioned problem so far, and plans to knock on the industry development through 10 different strategies.

• The Journal of the Convergence on Culture Technology
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• v.7 no.2
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• pp.351-357
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• 2021

At the time when interest in the use of big data is rising in the face of the technological paradigm shift of the 4th industrial revolution, interest in the use of patented big data is increasing, especially as the proportion of intangible assets of companies increases. In addition to quantitative information, patent data contains various information such as unstructured text such as title, abstract, claim, citation and citation relations, drawings, and technology classification. It is judged that the use of treatment is important. Therefore, in this study, in order to systematically operate the innovative patent strategy framework and to secure a competitive advantage by strengthening the fundamental technological competitiveness of the company, we propose a method of using patent big data centering on the case of Company A, and verify its validity. I would like to suggest some implications. Through this, it is intended to raise awareness of the use of patent big data, and to suggest ways to use patent big data in connection with the company's company-wide strategy, business strategy, and functional strategy.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.16 no.5
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• pp.152-161
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• 2017

The CBTC(Communication based Train Control) system employed on 2.4GHz ISM band adopt the IEEE802.11.x standard. Therefor communication time delay, distortion and data losses will be produced. To overcome these problems, CPSK(Continuous Phase shift keying) modulated DS/SS(Direct Sequence Spread Spectrum) transmitter with 908MHz carrier frequency is proposed. Through the eye diagram and scatter diagram the performance is analysed. And the phase noise characteristic of the local oscillator is measured about 60dBc/Hz, this means that the phase noise performance is very good compared to the signal. Reference frequency suppression of the 2nd local oscillator is 50dB below compared to the signal. Through the polarities of the complementary signal equally probable, the line spectrum of the output spectrum is eliminated. The nonlinear effects which introduce the spectral spreading or spectral regeneration is reduced remarkably.