• Journal of Plant Biotechnology
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• v.38 no.1
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• pp.87-93
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• 2011

S-Adenosylmethionine decarboxylase (SAMDC; EC 4.1.4.50), a key enzyme for polyamines biosynthesis, was tightly regulated for homeostatic levels. Carnation SAMDC gene (CSDC9) has an small upstream open reading frame (uORF) of 54 amino acids in 5'-leader sequence. To explore the functional mechanism of uORFs in controlling translation, we used a GUS reporter gene driven with the 35S promoter and uORF region of SAMDC gene for making transgenic tobacco plants. In our experiment, there were a translational inhibition of its downstream GUS ORF by SAMDC uORF sequence or SAMDC uORF protein. Expecially, translational inhibition was most effective in point-mutated construct, in which the start codon was changed. Therefore, this results suggested the ribosomal stalling might be involved in this translational inhibitory process. The frame shift in amino acid sequence of SAMDC uORF with start codon and stop codon resulted in a moderate increasing in GUS activity, suggesting the native amino acid sequence was important for a function as a translational inhibitor. Also, we showed that the production of GUS protein was significantly inhibited in the presence of the small uORF using histochemical analysis of GUS expression in seedlings and tobacco flowers. Importantly, the small uORF sequence induced a real peptide of 5.7 kDa, which was provided the presence of SAMDC uORF peptide band using an in vitro transcription/translation system. The peptide product of uORF might interact with other components of translational machinery as well as polyamines, which was resulted from that polyamine treatment was inhibited GUS protein band in SDS-PAGE experiment.

• The Journal of the Korea Contents Association
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• v.10 no.9
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• pp.243-250
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• 2010

With the help of digital technology, contents production process has become flexible and transmedia situation has been in the rise. Transmedia contents is a new type of contents being based on the various media platform and establishes a consistent story world. They are newly appeared mode of contents in the age of digital convergence. Users reading and watching the several versions of story in the transmedia contents can create a comprehensive contents experience and deepen it. Developing their own aesthetics and meaning of media text, transmedia contents is changing the textuality of digital contents, contents production process and contents using behaviors and contexts.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.16 no.1
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• pp.5-26
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• 2005

Sookmyung Women's University Library developed a Cyber Learning Program for Information Literacy Education in the Fall semester of 2004. The program was enforced as a pass/fail prerequisite of the "Reading & Writing", a compulsory course for the first-year students. The paper describes why and how the university reached to the decision to initiate such a program. It identifies the factors which contributed to the early success of the program by analyzing the contents development process and the actual course management process. Suggestions for further developments is made by analyzing the technical and procedural difficulties faced using the Learning Management System(LMS).

• Korean Institute of Interior Design Journal
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• no.11
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• pp.20-25
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• 1997

The purpose of this study is to find out the correlation between Truth(道) and dimension through the understanding of architectural point of value in the aspect of correlation between modern western thoughts and the thoughts of Truth and also through the reading of what kind of composite trend of the Truth can be seen in a Korea traditional house. This stduy takes the following procedure with reference books and traditional house that we have. 1) Making foundation for this study by finding out the fundamental meaning of the thoughts of Truth through the comparing and analyzing between modern western thoughts and the thoughts of Truth. 2) Reviewing the understanding of dimension in the thoughts of Truth. 3) Traslation of the organic correlation through the analysis of composition, placement and characteristics of dimension in a real present traditional Korea house. 4) Finding out the meaning of each dimension through the adaptation of the fundamental rules of nature of Jane(藏:store), Sang(生:life), Jang(長:long), Su(收obtain) to the dimension composite of a traditional house and concluding that the cyclical process in the oriental thoughts could be made.

• BMB Reports
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• v.38 no.2
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• pp.206-210
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• 2005

Gonadogenesis is a complicated process which involves multi-gene interactions. A rainbow trout (Oncorhynchus mykiss) gene spermatogenesis associated 4 (SPATA4) was cloned and characterized from adult rainbow trout testis. The cDNA sequence of rainbow trout SPATA4 contains an open reading frame of 1, 081 nucleatides encoding a putative protein of 259 amino acids. The putative protein from rainbow trout shares a 76.8% homology with zebrafish SPATA4. No trans-membrane regions or signal peptide were detected using bioinformatics methods. Subcellular localization analysis revealed that rainbow trout SPATA4 was a nuclear protein with highest possibility (39.1%). Multi-tissue reverse transcriptase PCR (RT-PCR) was performed to examine the distribution of rainbow trout SPATA4 in eleven organs of adult rainbow trout. The result demonstrated that this gene express specifically in testis and slight amount of expression was detected in ovary. Further analysis of SPATA4 characterization and function in rainbow trout may provide insight into the understanding of gonadogenesis process.

• Research in Mathematical Education
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• v.9 no.3 s.23
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• pp.295-303
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• 2005

Students' mathematics application ability is a hot spot which mathematics education pays attention. So, I have carried on the test of mathematics application and the questionnaire survey to student of Senior One of the two middle schools. Through the test and the investigation, I discover that the level of the students' mathematics application ability is not high. To the simple application question, the difference of the male and the female is not big, but to the difficult application question, the male and the female has the remarkable difference. The students' lack of flexible and the original cognition monitoring ability in mathematics application process are afraid of studying mathematics application since childhood and lack of the training of this aspect. They thought mathematics foundation and reading understood ability is the key of mathematics application. Through analysis result of investigation, this paper proposed the way to raise mathematics application ability of students.

• Journal of the Ergonomics Society of Korea
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• v.8 no.2
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• pp.19-26
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• 1989

This study, as a part of integrated human-robot ergonomics, improves the robot motion control on the robot task in the TOES/WCS whose purpose is improving the teaching task constructed in the previous study. First, the updated combined fuzzy process using a new membership function with Weber's law is constructed for the purpose of coordinate reading of the end points in the macro motion control. Second, an algorithm using the geometric analysis is desinged in order to calculate position values and posture values of the robot joints. Third, the MGSLM method is designed to remove unnecessary the robot motion control caused by the GSLM method in the micro motion control. Consequently, proposed methods in this study lessen burdcn of a human of an improvement of the robot motion control and reduce the teaching time of a human operator and inaccuracy of the teaching task, which contribute to the integrated human-robot ergonomics.

• Proceedings of the KSPS conference
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• 2005.04a
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• pp.29-33
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• 2005

This study aims to establish a speech corpus for Korean as a foreign language (L2 Korean Speech Corpus, L2KSC) and to examine the aspects of the foreign learners acquisition of the phonetic and phonological systems in the Korean Language. In the first year of this project, L2KSC will be established through the process of reading list organizing, recording, and slicing, and the second year includes an in-depth study of the aspects of foreign learners Korean acquisition and a contrastive analysis of phonetic and phonological systems. The expectation is that this project will provide significant bases for a variety of fields such as Korean education, academic research, and technological development of phonetic information.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.24 no.11
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• pp.2732-2741
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• 2000

Combination of magnetic recording technology and optical recording technology such as Near Field Optical Recording is watched recently. In order to accomplish this technology, the development of an electromagnetic driven mm-sized mirror shifting laser beam in track direction have to needed. In Near Field Optical Recording System, shifting laser beam in track direction mean as fine tracking and means as coarse tracking. Therefore in Near Field Optical Recording, 2-stage actuator is composed of servo controller in reading or recording information on disc layer. In our research, through design and simulation process of driven mm-sized mirror, we arrange systematically design process of driven mm-sized mirror having good frequency transfer characteristics. Design and simulation processes included modal analysis of spring, calculation of magnetic moment according to the number of turns and geometric configuration of coil and magnetic circuit analysis meaning that calculation of magnetic flux density in air gap of magnetic circuit. After that we design and make parts of driven mm-sized mirror, assemble and evaluate our electriomagnetic driven mm-sized mirror. we compared design values with actual characteristic values and present solution scheme. Through these processes we performed manufacturing of an electromagnetic driven mm-sized mirror having good frequency-domain characteristics and high sensitivity characteristics.

• Journal of Korean Society of Water and Wastewater
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• v.31 no.3
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• pp.249-256
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• 2017

Recently, application areas based on M2M (Machine-to-Machine communications) and IoT (Internet of Things) technologies are expanding rapidly. Accordingly, water flow and water quality management improvements are being pursued by applying this technology to water and sewage facilities. Especially, water management will collect and store accurate data based on various ICT technologies, and then will expand its service range to remote meter-reading service using smart metering system. For this, the error in flow rate data transmitting should be minimized to obtain credibility on related additional service system such as real time water flow rate analysis and billing. In this study, we have identified the structural problems in transmitting process and protocol to minimize errors in flow rate data transmission and its handling process which is essential to water supply pipeline management. The result confirmed that data acquisition via communication system is better than via analogue current values and pulse, and for communication method case, applying the industrial standard protocol is better for minimizing errors during data acquisition versus applying user assigned method.