BMB Reports

Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
권호 표지
DOI QR코드

DOI QR Code

Cloning and Expression Analysis of Gonadogenesis-associated Gene SPATA4 from Rainbow Trout (Oncorhynchus mykiss)

  • Liu, Bowen (Department of Biological Sciences and Biotechnology, Tsinghua University) ;
  • Liu, Shangfeng (Department of Biological Sciences and Biotechnology, Medical School, Tsinghua University) ;
  • He, Shan (Department of Biological Sciences and Biotechnology, Tsinghua University) ;
  • Zhao, Ying (Department of Biological Sciences and Biotechnology, Tsinghua University) ;
  • Hu, Hongxia (Beijing Fishery Institute) ;
  • Wang, Zhao (Department of Biological Sciences and Biotechnology, Medical School, Tsinghua University)
  • Published : 2005.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Gonadogenesis is a complicated process which involves multi-gene interactions. A rainbow trout (Oncorhynchus mykiss) gene spermatogenesis associated 4 (SPATA4) was cloned and characterized from adult rainbow trout testis. The cDNA sequence of rainbow trout SPATA4 contains an open reading frame of 1, 081 nucleatides encoding a putative protein of 259 amino acids. The putative protein from rainbow trout shares a 76.8% homology with zebrafish SPATA4. No trans-membrane regions or signal peptide were detected using bioinformatics methods. Subcellular localization analysis revealed that rainbow trout SPATA4 was a nuclear protein with highest possibility (39.1%). Multi-tissue reverse transcriptase PCR (RT-PCR) was performed to examine the distribution of rainbow trout SPATA4 in eleven organs of adult rainbow trout. The result demonstrated that this gene express specifically in testis and slight amount of expression was detected in ovary. Further analysis of SPATA4 characterization and function in rainbow trout may provide insight into the understanding of gonadogenesis process.

Keywords

References

  1. Allendorf, F. W. and Thorgaard, G. H. (1984) Tetraploidy and the evolution of salmonid fishes; in The Evolutionary Genetics of Fishes, Turner, B. J. (ed.), pp. 1-53, Plenum Press, New York, USA
  2. Baroiller, J. F. and Guiguen, Y. (2001) Endocrine and environmental aspects of sex differentiation in gonochoristic fish. EXS. 91, 177-201
  3. Bromage, N., Jones, J., Randall, C., Thrush, M, Davies, B., Springate, J., Duston, J. and Barker, J. (1992) Broodstock management, fecundity, egg quality and the timing of egg production in the rainbow trout (Oncorhynchus mykiss). Aquaculture. 100, 141-166 https://doi.org/10.1016/0044-8486(92)90355-O
  4. Bull, J. J. (1983) Evolution of Sex Determining Mechanisms, Menlo, P. (ed.), pp. 1-336, Benjamin/Cummings, San Diego, USA
  5. Douglas, S. E., Gallant, J. W., Bullerwell, C. E., Wolff, C., Munholland, J. and Reith, M. E. (1999) Winter flounder expressed sequence tags: establishment of an EST database and Identification of novel fish genes. Mar. Biotechnol. 1, 458-464 https://doi.org/10.1007/PL00011802
  6. Eddy, E. M. (2002) Male germ cell gene expression. Recent Prog. Horm. Res. 57, 103-128 https://doi.org/10.1210/rp.57.1.103
  7. Emanuelsson, O., Nielsen, H., Brunak, S. and von Heijne, G. (2000) Predicting subcellular localization of proteins based on their N-terminal amino acid sequences. J. Mol. Biol. 300, 1005-1016 https://doi.org/10.1006/jmbi.2000.3903
  8. Jiang, H., Li, L. Y. and Lu, G. X. (2001) Molecular cloning of genes related to apoptosis in spermatogenic cells of mouse. Chin. J. Biochem. Biophys. 33, 421-425
  9. Knower, K. C., Kelly, S. and Harley, V. R. (2003) Turning on the male-SRY, SOX9 and sex determination in mammals. Cytogenet. Genome Res. 101, 185-198 https://doi.org/10.1159/000074336
  10. Liu, S., Govoroun, M., D'Cotta, H., Ricordel, M. J., Lareyre, J. J., McMee, O. M., Smith, T., Nagahama, Y. and Guiguen, Y. (2000) Expression of cytochrome P450 (11beta) (11betahydroxylase) gene during gonadal sex differentiation and spermatogenesis in rainbow trout, Oncorhynchus mykiss. J. Steroid Biochem. Mol. Biol. 75, 291-298 https://doi.org/10.1016/S0960-0760(00)00186-2
  11. Liu, S. F., Li, L. Y., Fu, J. J., Liu, G., Xing, X. W. and Lu, G. X. (2002) Rapid identification of human testis spermatocyte apoptosis-related gene, TSARG2, by nested PCR and draft human genome searching. Chin. J. Biochem. Biophys. 34, 378- 382
  12. Liu, S. F., Li, L. Y., Mo, Y. Q., Fu, J. J., Liu, G., Xing, X. W. and Lu, G. X. (2003) Molecular cloning and expression in cryptorchid testis of SRG2 from a mouse testis spermatocyte apoptosis-related gene. Yi Chuan Xue Bao 30, 943-948
  13. Liu, S. F., Lu, G. X., Liu, G., Xing, X. W., Li, L. Y. and Wang, Z. (2004) Cloning of a full-length cDNA of human testisspecific spermatogenic cell apoptosis inhibitor TSARG2 as a candidate oncogene. Biochem. Biophys. Res. Commun. 319, 32- 40 https://doi.org/10.1016/j.bbrc.2004.04.160
  14. Liu, S. F., He, S., Liu, B. W., Zhao, Y. and Wang, Z. (2004) Cloning and characterization of testis-specific spermatogenesis associated gene homologous to human SPATA4 in rat. Biol. Pharm. Bull. 27, 1867-1870 https://doi.org/10.1248/bpb.27.1867
  15. Marchand, O., Govoroun, M., D'Cotta, H., McMeel, O., Lareyre, J., Bernot, A., Laudet, V. and Guiguen, Y. (2000) DMRT1 expression during gonadal differentiation and spermatogenesis in the rainbow trout, Oncorhynchus mykiss. Biochim. Biophys. Acta. 1493, 180-187 https://doi.org/10.1016/S0167-4781(00)00186-X
  16. Nakai, K. and Horton, P. (1999) PSORT: a program for detecting sorting signals in proteins and predicting their subcellular localization. Trends Biochem. Sci. 24, 34-36 https://doi.org/10.1016/S0968-0004(98)01336-X
  17. Nakanishi, Y. (1995) Study on the mammalian spermatogenic pathway. Yakugaku Zasshi 115, 420-430
  18. Nielsen, H., Engelbrecht, J., Brunak, S. and von Heijne, G. (1997) Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10, 1-6 https://doi.org/10.1093/protein/10.1.1
  19. Sigrist, C. J., Cerutti, L., Hulo, N., Gattiker, A., Falquet, L., Pagni, M., Bairoch, A. and Bucher, P. (2002) PROSITE: a documented database using patterns and profiles as motif descriptors. Brief. Bioinformatics 3, 265-274 https://doi.org/10.1093/bib/3.3.265
  20. Takamatsu, N., Kanda, H., Tsuchiya, I., Yamada, S., Ito, M., Kabeno, S., Shiba, T. and Yamashita, S. (1995) A gene that is related to SRY and is expressed in the testes encodes a leucine zipper-containing protein. Mol. Cell. Biol. 15, 3759-3766
  21. Thorgaard, G. H. (1977) Heteromorphic sex chromosomes in male rainbow trout. Science 196, 900-902 https://doi.org/10.1126/science.860122
  22. Thorgaard, G. H. and Gall, G. A. E. (1979) Adult triploids in a rainbow trout family. Genetics 93, 961-973
  23. Thorgaard, G. H., Bailey, G. S., Williams, D., Buhler, D. R., Kaattari, S. L., Ristow, S. S., Hansen, J. D., Winton, J. R., Bartholomew, J. L., Nagler, J. J., Walsh, P. J., Vijayan, M. M., Devlin, R. H., Hardy, R. W., Overturf, K. E., Young, W. P., Robison, B. D., Rexroad, C. and Palti, Y. (2002) Status and opportunities for genomics research with rainbow trout. Comp. Biochem. Physiol. B. Biochem. Mol. Biol. 133, 609-646 https://doi.org/10.1016/S1096-4959(02)00167-7
  24. Venkatesh, B. (2003) Evolution and diversity of fish genomes. Curr. Opin. Genet. Dev. 13, 588-592 https://doi.org/10.1016/j.gde.2003.09.001

Cited by

  1. Analysis of protein expression in zebrafish during gonad differentiation by targeted proteomics vol.193, 2013, https://doi.org/10.1016/j.ygcen.2013.07.020
  2. Molecular pathways associated with the intersex condition in rainbow darter (Etheostoma caeruleum) following exposures to municipal wastewater in the Grand River basin, ON, Canada. Part B vol.159, 2015, https://doi.org/10.1016/j.aquatox.2014.11.022
  3. Cloning and characterization of chicken SPATA4 gene and analysis of its specific expression vol.306, pp.1-2, 2007, https://doi.org/10.1007/s11010-007-9556-9
  4. A yeast two hybrid screen identifies SPATA4 as a TRAPP interactor vol.585, pp.17, 2011, https://doi.org/10.1016/j.febslet.2011.07.040
  5. to discover candidate sex-related genes vol.6, pp.2167-8359, 2018, https://doi.org/10.7717/peerj.5389
  6. vol.3, pp.6, 2013, https://doi.org/10.1534/g3.113.006338