• 제목/요약/키워드: the moon

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녹문 임성주의 일원적(一原的) 심성론(心性論) 연구 (The study of monistic mind-nature theory of Nog-Moon Yim Seong-Joo)

  • 이명심
    • 한국철학논집
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    • 제41호
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    • pp.185-222
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    • 2014
  • 녹문 임성주는 18세기 조선후기 철학자이다. 녹문은 호락논쟁이 리(理)와 기(氣)를 분리해 보는 데서 발생했다고 판단하고, 자신의 관점에 따라 새로운 리기론과 심성론을 정립한다. 녹문 철학의 종지는 이기동실(理氣同實) 심성일치(心性一致)이고, 녹문은 담일한 기 본체를 바탕으로 자신의 종지를 논증한다. 그리하여 리기는 이원이 아니라 기일원으로 환원되고, 심성은 심으로 환원된다. 심의 존재근거는 기이기 때문에, 결국 녹문의 본체론은 기일원론(氣一原論)이 되는 것이다. 녹문은 정명도의 일원적 방법론을 원용하여, 자신의 거대한 일원론 철학을 제출한다. 본체의 보편성과 현상의 다양성을 '일원분수(一原分殊)'라는 논리구조로 설명한다. 녹문 철학의 특징은 일원과 분수가 모두 본연으로 동일하다는 데 있다. 즉, 체용일원 내지 본말일치를 의미한다. 심과 성도 본연으로 일치하고, 기와 심과 성도 본연으로 일치하고, 몸과 마음도 본연으로 일치한다는 내용이다. 성리학의 용어로 하면, 기질이 본연과 다르지 않다는 뜻이다. 그리하여 나는 녹문의 철학을 '성인(聖人)의 관점에서 건립한 철학' 이라고 정의 내린다.

Production of dTDP-4-keto-6-deoxy-D-glucose by Immobilization of dTDP-D-glucose 4,6-dehydratase

  • Kharel, Mandan-Kumar;Liou, Kwang-Kyoung;Sohng, Jae-Kyung;Lee, Hei-Chan
    • Journal of Microbiology and Biotechnology
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    • 제14권2호
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    • pp.297-301
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    • 2004
  • The dTDP-D-glucose 4,6-dehydratase from Salmonella enterica was immobilized using covalent binding to cyanogen bromide activated sepharose. The immobilized enzyme was used to produce dTDP-4-keto-6-deoxy-D-glucose, a key sugar intermediate that can be used economically to produce diverse classes of unusual sugars appended in various antibiotics. The enzyme was immobilized on the sepharose after activation with cyanogen bromide. The maximum immobilization (80.03%) was achieved after 14 h of coupling. The covalently immobilized enzyme was stable, and an average of 78.4 % conversion was achieved until 120 h of immobilization when it was repeatedly used. Similar conversion was noticed for the first batch using the enzyme entrapped-hydrogel but activity was gradually decreased in the following batches. The production of dTDP-4-keto-6-deoxy-D-glucose by using an immobilized enzyme has high potential for commercial application.

초등학생들의 달의 위상변화에 대한 개념 유형과 수업 방법의 제안 및 효과 (Conception Types of Elementary School Students about the Moon Phase Changes and the Suggestions and Effects of Teaching Methods)

  • 손준호
    • 한국과학교육학회지
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    • 제35권2호
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    • pp.289-301
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    • 2015
  • 이 연구에서는 삼구의를 활용한 기존의 수업 방식으로는 학생들이 달의 위상변화의 원리를 정확하게 이해할 수 없을 뿐만 아니라 교사들 조차도 제대로 된 이해가 부족한 채 지도하고 있음에 주목하였다. 따라서 삼구의를 활용한 달의 위상변화 학습에서 초등학생들이 이해하고 있는 유형을 161명의 응답 결과를 토대로 분류하고, 기존의 연구에서 개발된 학습 자료를 재구성한 교수 학습 방법을 활용함으로써 그들의 이해 유형이 어떻게 변하였는지 확인하였으며, 129명 학생들의 학업성취도에는 어떠한 효과를 미치는지 검증하였다. 그리고 교사와 학생 총 8명을 대상으로 새로운 교수 학습 방법에 대해 어떠한 생각을 갖고 있는지 심층 면담을 통해 확인하였다. 그 결과, 학생들은 크게 C 유형과 W 유형을, 세부적으로는 W1, W2, W3, W4, W5 유형으로 나눌 수 있었는데 보름달 보다는 초승달과 상현달의 위상 원리를 더 어려워하였다. 그리고 재구성한 교수 학습 방법을 활용해 보충수업을 실시한 65명의 실험집단 학생들이 지도서의 내용으로 보충수업을 실시한 64명의 비교집단 학생들보다 학업성취도 사후 검사뿐만 아니라 추후 검사에서도 효과가 있었다. 심층 면담에서도 교사 학생 모두 이 연구에서 제시한 교수 학습 방법에 대해 긍정적인 답변을 해 주었다. 결론적으로 이 연구를 통해 달의 위상변화와 관련된 활동을 삼구의를 활용해 학습할 때는 결과만을 암기하는 학습에서 벗어나 원리를 정확하게 이해할 수 있도록 초등학생들에게 도움을 주는 단계별 학습 자료를 적극 활용할 것을 강조하였다.

Characterization of RbmD (Glycosyltransferase in Ribostamycin Gene Cluster) through Neomycin Production Reconstituted from the Engineered Streptomyces fradiae BS1

  • Nepal, Keshav Kumar;Oh, Tae-Jin;Subba, Bimala;Yoo, Jin Cheol;Sohng, Jae Kyung
    • Molecules and Cells
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    • 제27권1호
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    • pp.83-88
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    • 2009
  • Amino acid homology analysis predicted that rbmD, a putative glycosyltransferase from Streptomyces ribosidificus ATCC 21294, has the highest homology with neoD in neomycin biosynthesis. S. fradiae BS1, in which the production of neomycin was abolished, was generated by disruption of the neoD gene in the neomycin producer S. fradiae. The restoration of neomycin by self complementation suggested that there was no polar effect in the mutant. In addition, S. fradiae BS6 was created with complementation by rbmD in S. fradiae BS1, and secondary metabolite analysis by ESI/MS, LC/MS and MS/MS showed the restoration of neomycin production in S. fradiae BS6. These gene inactivation and complementation studies suggested that, like neoD, rbmD functions as a 2-N-acetlyglucosaminyltransferase and demonstrated the potential for the generation of novel aminoglycoside antibiotics using glycosyltransferases in vivo.

서울시(市) 대기중(大氣中) 유해(有害) 부유분진(浮遊粉塵) 성분(成分)에 관(關)한 조사연구(調査硏究) -부유분진중(浮遊粉塵中)의 Benzo(a)pyrene에 관(關)하여- (Studies on Benzo(a)pyrene of the Suspended Particulate in Atmosphere of Seoul City)

  • 권숙표;정용;임동구
    • Journal of Preventive Medicine and Public Health
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    • 제11권1호
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    • pp.65-75
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    • 1978
  • This study was carried out to investigate air pollution by total suspended particulate(T.S.P.), benzene soluble matter and benzo(a) pyrene in Seoul city. The sampling areas were divided into commercial(Kwang Hwa Moon), indus-trial(Ku Ro Dong) and residensial area(Shin Chon). Sampling was undertaken by High Voulme Air Sampler for four seasons from January 1917 to November 1977. The T.S.P. was extracted with Soxhlet apparatus by benzene and benzo(a) pyrene was separated by column chromatography and thin layer chromatography. The concentrations of benzo(a) pyrene were measured by means of fluorophotometer, and following results were obtained. 1. Arithmetic average concentration for 1-day averaging time of total suspended particulate were $275.6ug/m^3$ in Kwang Hwa Moon, $325.9ug/m^3$ in Ku Ro Dong and $193.0ug/m^3$ in Shin Chon. 2. The seasonal variance of total suspended parti-culate at Ku Ro Dong and Shin Chon were $102.7ug/m^3\;99.6ug/m^3$ respectively and at Kwang Hwa Moon $39.9ug/m^3$. And the concentration of autumn is higher than of that spring at Ku Ro Dong and at Shin Chon, but at Kwang Hwa Moon, the seasonal variance is very little. 3. The concentrations of 50% frequency from geometric mean for 1-day averaging time were $264ug/m^3,\;300ug/m^3\;and\;178ug/m^3$ at Kwang Hwa Moon, Ku Ro Dong and Shin Chon. And geometric standard deviation were 1.27, 1.38 and 1.41 respectively. 4. The concentrations of benzene soluble mater were $26.9ug/m^3$ at Kwang Hwa Moon, $22.7ug/m^3$ at Ku Ro Dong and $15.5ug/m^3$ at Shin Chon, and the ratios to the T.S.P. were 9.8%(range 5.6-14.8%), 7.0%(range 2.4-14.4%) and 8.0%(range 5.5-22.1%) respectively. 5. The concentrations of benzo(a) pyrene were $8.5ug/m^3$ (range $0.8-29.9ug/m^3$) at Kwang Hwa Moon $10.9ug/m^3$(range $1.1-52.0ug/m^3$) at Ku Ro Dong and $5.8ug/m^3$(range $1.5-11.4ug/m^3$) at Shin Chon. 6. The results of this investigation were relatively high in compared with the recommended standards of suspended particulate in air of U.S. Environmental Protection Agency and observed levels of benzo(a)-pyrene in U.S. city.

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레이저 미세가공 기술을 이용한 초소형 전자빔 장치용 정전장 전자렌즈의 제작 (Fabrication of Electrostatic Electron Lens for Electron Beam Microcolumn using the Laser Micromachining)

  • 안승준;김대욱;김호섭;김영정;이용산
    • 한국재료학회지
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    • 제11권9호
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    • pp.792-796
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    • 2001
  • For electron beam lithography and SEM(scanning electron microscopy) applications, miniaturized electrostatic lenses called a microcolumn have been fabricated. In this paper, we report the fabrication technique for 20~30$\mu\textrm{m}$ apertures of electron lenses based on silicon and Mo membrane using an active Q-switched Nd:YAG laser. Experimental conditions of laser micromachining for silicon and Mo membrane are improved. The geometrical structures, such as the diameter and the preciseness of the micron-size aperture are dependent upon the total energy of the laser pulse train, laser pulse width, and the diameter of laser spot.

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Cloning and Functional Characterization of the Germacradienol Synthase (spterp13) from Streptomyces peucetius ATCC 27952

  • Ghimire, Gopal Prasad;Oh, Tae-Jin;Lee, Hei-Chan;Kim, Byung-Gee;Sohng, Jae-Kyung
    • Journal of Microbiology and Biotechnology
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    • 제18권7호
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    • pp.1216-1220
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    • 2008
  • Sequence analysis of the metabolically rich genome of Streptomyces peucetius ATCC 27952 revealed a 2,199 bp sesquiterpene alcohol (germacradienol) synthase-encoding gene from the germacradienol synthase/terpene cyclase gene cluster. The gene was named spterp13, and its putative function is as a germacradienol synthase/terpene cyclase. The amino acid sequence of Spterp13 shows 66% identity with SAV2163 (GeoA) from S. avermitilis MA4680 and 65% identity with SCO6073 from S. coelicolor A3(2), which produces germacradienol/geosmin. The full-length recombinant protein was heterologously expressed as a his-tagged fusion protein in Escherichia coli, purified, and shown to catalyze the $Mg^{2+}$-dependent conversion of farnesyl diphosphate to the germacradienol, which was verified by gas chromatography/mass spectrometry.