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Effects of Fermented Extracts Zizyphus jujuba Fruits and Leaves Mixture on Hyperlipidemia in Rats Induced by High Fat Diet (대추과육과 대추잎 혼합발효추출물이 고지방식이로 유발된 고지혈증 흰쥐에 미치는 영향)

  • Chong, MyongSoo;Cho, Hwaeun;Kim, Heaja;Choi, YunHee
    • Journal of Society of Preventive Korean Medicine
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    • v.19 no.1
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    • pp.117-130
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    • 2015
  • Objective : This study was designed to investigate the effects of non fermented Zizyphus. jujuba mixture (mixed fruit and leaf : ZM) and fermented Z. jujuba mixture(FZM) on fed high-fat diet induced hyperlipidemic rats for development medicinal food. Method : The extracts prepared for Zizyphus. jujuba mixture(ZM) and fermented Z. jujuba mixture(FZM) with Lactobacillus plantarum and Saccharomyces cerevisiae. Experimental group was divided into normal control group(NC group) and high fat diet groups. The high fat diet groups fed high fat diet 2 weeks after was sub-divided into high fat diet control group(HC group), high fat diet positive control group(HPC group), non fermented Z. jujuba mixture extract group(ZM group) and fermented Z. jujuba mixture extract group(FZM group). NC and HC group was orally administerd of 0.9% saline, HPC group administerd lovastatin diluted in 0.9% saline at a dose of 10 mg/kg BW, ZM and FZM groups was administerd each extracts diluted in 0.9% saline at a dose of 300 mg/kg BW once a day on a fixed time for 4 weeks. In the present study we measured organ weight, epididymal fat tissue weight, concentration of serum lipids, hepatic lipids, MDA contents in liver tissue and metabolic variables in serum. Results : ZM and FZM suppressed testis weight loss and FZM decreased epididymal fat tissue weight to level of NC group in high-fat diets. ZM and FZM did not influence on serum cholesterol level, but prominently decreased serum triglyceride concentration compared with HC group, and FZM diminished hepatic triglyceride same as serum. ZM and FZM did not impair liver and kidney function and influence positive effects through by suppression of elevation lipid level. Conclusion : These results suggested that Z. jujuba mixture(ZM, FZM) should be useful developing medicinal food for prevention and improvement of hyperlipidemia and FZM is more suitable agent than ZM.

Occurrences of Insect Pests on Fruit-producing Mulberry Plants in Jeonbuk Province (전북지역 오디 생산용 뽕나무 해충의 발생양상)

  • Lim, Ju-Rak;Moon, Hyung-Cheol;Kim, Dong-Wan;Kwon, Suk-Ju;Han, Soo-gon;Kwak, Dong-Ok
    • Korean journal of applied entomology
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    • v.56 no.2
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    • pp.203-212
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    • 2017
  • Thirty-three species of insect pests including of two-spotted spider mite, Tetranychus urticae Koch, and air-breathing land snail, Acusta despecta Sowerby, were observed on mulberry for producing mulberry fruits in Jeonbuk province from 2015 to 2016. Of these, 8 species of insect pests, including of mulberry thrips, Pseudodendrothrips mori Niwa, are regarded as major insect pests. Of the 25 species observed before harvesting, 6 species-P. mori, Anomoneura mori, Pseudaulacaspis pentagona, Baris deplanata, Menophra atrilineata and T. urticae-are regarded as major insect pests. In contrast, of the 20 species observed after harvesting, 5 species-P. mori, P. pentagona, Glyphodes pyloalis Walker, Hyphantria cunea Drury and T. urticae-are regarded as major insect pests. Eight of the major insect pest species and nine other insect pest species-including Hexcacentrus unicolor Serville, Malcus japonicus Ishihara et Hasegawa, Halyomorpha halys stal, Xylosandrus germanus Blandford, four species of Repidoptera (Bombyx mandarina Moore, Euproctis subflava Bremer, Acronicta major Bremer, Agrotis trifurca Butler), and Acusta despecta Sowerby-were observed at all sites studied, although occurrence of the insect pests observed on mulberry varied from site to site.

Development of Real-time Quantitative PCR Assay based on SYBR Green I and TaqMan Probe for Detection of Apple Viruses (사과 바이러스 검정을 위한 SYBR Green I 및 TaqMan probe 기반의 real-time PCR 검사법 개발)

  • Heo, Seong;Chung, Yong Suk
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.65 no.4
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    • pp.496-507
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    • 2020
  • Virus infections of apples result in lowered commercial qualities such as low sugar content, weakened tree vigor, and malformed fruits. An effective way to control viruses is to produce virus-free plants based on the development of an accurate and sensitive diagnostic method. In this study, real-time PCR assays based on SYBR Green I and TaqMan probes were developed for detecting ASGV, ASPV, and ApMV viruses. These methods can detect and quantify 103 to 1011 RNA copies/μL of each virus separately. Compared with methods with two different dyes, the SYBR Green I-based method was efficient for virus detection as well as for assay using the TaqMan probe. Field tests demonstrated that real-time PCR methods developed in this study were applicable to high-throughput diagnoses for virus research and plant quarantine.

Screening of Biological Activities to Different Ethanol Extracts of Rubus coreanus Miq. (복분자 에탄올 추출물의 생리활성 탐색)

  • Kwon, Ji-Wung;Lee, Hee-Kwon;Park, Hee-Jeon;Kwon, Tae-Oh;Choi, Heh-Ran;Song, Ji-Young
    • Korean Journal of Medicinal Crop Science
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    • v.19 no.5
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    • pp.325-333
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    • 2011
  • This study was carried out to evaluate biological activities to different ethanol extracts from unripened and ripened fruit (Rubus coreanus Miq.). 25% to 75% ethanol extracts of unripened and ripened fruits were similar to extract yield respectively. Yield of ethanol extract of ripened fruit were approximately 3 times higher than that of unripened fruit. 75% ethanol extract of unripened fruit showed the highest contents of total polyphenol ($180.04{\pm}0.41$ mg/g) and total flavonoid ($50.43{\pm}0.81$ mg/g). Contents of total polyphenol and total flavonoid of unripened fruit were about 2 times higher than those of ripened fruit. $IC_{50}$ values of DPPH radical scavenging activity and linoleic acid peroxidation inhibition activity of BHA and ${\alpha}$-tocopherol showed $13.19{\pm}0.21$ and $18.16{\pm}0.23{\mu}g/m{\ell}$, $4.25{\pm}0.04{\mu}g/m{\ell}$ and $5.56{\pm}0.10{\mu}g/m{\ell}$ respectively, but 75% ethanol extract of unripened fruit showed the lowest $23.85{\pm}0.10{\mu}g/m{\ell}$ and $7.34{\pm}0.07{\mu}g/m{\ell}$ among other all extracts. $IC_{50}$ values of LDL (low density lipoprotein) oxidation inhibition activity and ${\alpha}$-glucosidase inhibitory activity of 75% ethanol extract of unripened fruit showed the lowest $1.04{\pm}0.04{\mu}g/m{\ell}$ and $7.21{\pm}0.13{\mu}g/m{\ell}$ among other all extracts respectively. Specifically, 75% ethanol extract of unripened fruit has relatively better biological activities than other ethanol extracts, it could be potentially used as bioactive source for health functional foods.

Antioxidative Activity and Chemical Characteristics of Leaf and Fruit Extracts from Thuja orientalis (측백나무 잎.열매 추출물의 이화학적 특성 및 항산화 효과)

  • Ahn, Hee-Young;Heo, Su-Jin;Kang, Min-Jung;Lee, Jae-Hong;Cha, Jae-Young;Cho, Young-Su
    • Journal of Life Science
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    • v.21 no.5
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    • pp.746-752
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    • 2011
  • The contents of bioactive materials (e.g. polyphenolics compounds, flavonoids, minerals, and fatty acids) and antioxidative activities (DPPH (${\alpha}$,${\alpha}$'-diphenyl-${\beta}$-picrylhydrazyl) free radical scavenging activity, peroxidation of linoleic acid and rat hepatocyte microsome, and Fe/Cu reducing power) were tested by in vitro experimental models using water, ethanol and methanol extracts of leaves (TOL) and fruits (TOF) from Thuja orientalis. Methanol extract from TOL showed the highest extraction yield (12.90%) as well as contents of polyphenolic compounds (16.02%) and flavonoids (0.25%). Major minerals were Ca, K, and Mg. Major fatty acids were palmitic and lauric acids in TOL and palmitic and decanoic acids in TOF. In oxidation of in vitro models using DPPH free radical scavenging activity, Fe/Cu reducing power, $Fe^{2+}$/ascorbate-induced linolenic acid peroxidation by ferric thiocyanate and thiobarbituric acid (TBA) methods, and autooxidation of rat hepatic microsomes membrane, anti-oxidative activities were stronger in all extracts of TOL than in those of TOF in a dose-dependent manner. From these results, methanol extract of TOL was shown to have the most potent anti-oxidative properties and the highes content of antioxidative compounds such as polyphenolic compounds and flavonoids.

Influence of Harvest Time after Freezing Damage on Fruit Quality during Storage of 'Fuji' Apples (동결피해 후의 수확시기가 사과 '후지' 품종의 저장 중 과실품질에 미치는 영향)

  • Kweon, Hun-Joong;Lee, Sang-Gyu;Park, Moo-Yong;Song, Yang-Yik;Nam, Jong-Chul;SaGong, Dong-Hoon
    • Horticultural Science & Technology
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    • v.28 no.6
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    • pp.990-995
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    • 2010
  • This study was conducted to compare differences in fruit quality by harvest time of 'Fuji' apple fruit that was frozen on tree by unusual low temperature in that air temperature was under $-3.5^{\circ}C$ for 7 hours. Fruits were harvested at 1 day before, and 2 days and 6 days after freezing damage, respectively. Harvest's soluble solid contents in all treatments was over $14^{\circ}Bx$. Firmness and titratable acidity of fruit harvested after freezing damage was lower than those of fruit harvested before freezing damage. During cold storage, ethylene production of fruit harvested after freezing damage was higher than that of fruit harvested before freezing damage. The reduction in the level of fruit quality during cold storage of fruit harvested after freezing damage was more serious than that of fruit harvested before freezing damage. The reduction of fruit quality during subsequent ambient temperature for 1 week after cold storage of fruit harvested after freezing damage was higher than that of fruit harvested before freezing damage. In comparison of treatments that were harvested at different times after freezing damage, ethylene production and reduction in the level of fruit quality until 8 weeks of cold storage of fruit harvested at 6 days after freezing damage was lower than that of fruit harvested at 2 days after freezing damage. However, this difference by harvest time after freezing damage disappeared after 8 weeks of cold storage. Incidence of flesh browning was not affected by freezing at air temperature under $-3.5^{\circ}C$ for 7 hours.

Exposure Assessment of Total Aflatoxin in Foods (식품중 총 아플라톡신의 노출량 평가)

  • Suh, Jung-Hyuk;Sho, You-Sub;Park, Seong-Soo;Choi, Woo-Jeong;Lee, Jong-Ok;Kim, Hee-Yun;Woo, Gun-Jo;Oh, Keum-Soon
    • Korean Journal of Food Science and Technology
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    • v.39 no.1
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    • pp.25-28
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    • 2007
  • A survey of total aflatoxin levels was conducted on 565 food samples (cereals, nuts, etc) collected in commercial markets. The determination of aflatoxins ($B_{1}$, $B_{2}$, $G_{1}$ and $G_{2}$) was performed using HPLC with fluorescence detector. The Limit of Detections (LODs) of the B group and G group were 0.05 ng/g and 0.07 ng/g, respectively. In addition, recoveries of rice, peanut butter, and red pepper flour were satisfactory. Total aflatoxin was detected 27 samples(4.8%) out of 565 samples. Incidence ratios in cereals, nuts, processed products, and other foods were 0.2, 0.4, 3.0 and 1.2%, respectively, but aflatoxin was not detected in pulse and dried fruits. The daily intake of total aflatoxin using food intakes was 0.04 ng/kg bw/day.

Characteristics of New Satsuma Mandarin Cultivar 'Sangdojosaeng' (온주밀감 신품종 '상도조생' 특성)

  • Park, Young Chul;Oh, Hyun Woo;Kang, Jong Hoon;Lee, Joong Seok;Chin, Seok Cheon;Kang, Sang Hoon;Kang, Sung Geun
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.143-147
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    • 2013
  • 'Sangdojosaeng' is a new cultivar from bud spot of Citrus unshiu 'Sasaki unshiu' which has late maturity habit. It was found in 1996, and primary selected and named as 'JARES108' in 1998. 'JARES108' was grafted on to adult satsuma mandarin trees in Seogwipo-si and Jeju-si in 2000. Four years survey on the characteristics of trees and fruits from 2003 to 2006 was carried and confirmed the superiority. Thus it was finally selected as 'Sangdojosaeng'. 'Sangdojosaeng' is a very early wase type satsuma mandarin. It shows relatively strong tree vigor among the very early wase type varieties and its branches are spreading. Its ripening time is late October, 25 days earlier than 'Sasaki unshiu'. Compared to 'Ueno wase' which has similar characteristics with 'Sangdojosaeng' such as ripening time, tree vigor, fruit type and peel color, its soluble solid content of fruit is $0.6^{\circ}Bx$ higher and acidity was a little lower than 'Ueno wase'. Because this cultivar has good quality and its tree makes vigorous growth among very early wase type varieties in Jeju, we expect to substitute it for some very early wase type varieties such as 'Miyamoto wase', 'Ichifumi wase', 'Yamakawa wase', etc.

Development of Suitable Alternative Substrates in Hydroponics of Sweet Pepper (착색단고추의 수경재배에 적합한 대체 배지 개발)

  • Bae, Jong-Hyang;Lee, Yong-Beom;Kim, Ho-Cheol;Cha, Seung-Hoon;Lee, Hye-Jin
    • Journal of Bio-Environment Control
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    • v.17 no.2
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    • pp.138-142
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    • 2008
  • To develop suitable alternative substrates in hydroponics of sweet pepper, changes of water contents of substrates and electric conductivity (EC) of drainage nutrient solution, growth and fruit characteristics according to the kind of coir substrates were investigated compared with rockwool. In all coir substrates compared with rockwool during growing period, water contents were higher, EC of drainage nutrient solution except for fiber 50% were lower, and the coefficient of variation were a little, especially were so in fiber 30% of coir substrates. Plant growth in fiber 30% substrate was better than others but there was no significant difference. Photosynthesis rate and chlorophyll content were best in fiber 50% substrate. Fruit weight was no significant difference, but LID ratio and shape of the fruits were good in fiber 50% substrate and rockwool as which were close to regular square. Therefore, it was estimated that mixed coir substrates with fiber $30{\sim}50%$ are enough in possibility as alternative substrate.

Analysis of Nitrate Contents of Korean Common Foods (상용식품 중의 질산염 함량 분석)

  • Kim, Bo-Young;Yoon, Sun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.6
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    • pp.779-784
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    • 2003
  • Nitrate a common component of man's chemical environment, is present in foods either naturally or in an additive introduced during processing of foods. Nitrate level of foods have been found depending on nitrosation of soils and other various environmental factors. Therefore data on nitrate contents of foods reported in other countries can not be adapted to Korean foods. Also data on nitrate levels of Korean foods available were reported 10 years ago, which would be assumed to differ from those of foods currently consuming by Koreans. The present study was attempted to determine nitrate contEnts of common foods. Based on National Nutrition Survey Report 143 food items out of 14 food groups were selected for this study Nitrate levels of common foods used in the study were from N.D (not detected) to 6733.33 mg/kg foods. Mean nitrate levels of food groups 27.2 mg/kg of cereal and grain products, 78.1 mg/kg in potatoes and potato products, 8.3 mg/kg in legumes and their products N.D of seeds and nuts, 1012.1 mg/kg of vegetables and their products,76.3 mg/kg fungi and mushrooms 42.2 mg/kg fruits,34.5 mg/kg of meat, poultry and their products,0 mg/kg in eggs, 23.9 mg/kg in fish, shellfishes and their products,23.0 mg/kg in seaweeds, 7.7 mg/kg milk and dairy products, 26.3 mg/kg in seasonings, 68.0 mg/kg in others.