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Protective Effect of Iminoctadine tris(albesilate) and Kresoxim-methyl Fungicides to Citrus Postharvest Diseases caused by Penicillium spp. (저장 감귤의 부패에 관여하는 Penicillium spp.에 대한 Iminoctadine tris(albesilate)와 Kresoxym-methyl의 방제 효과)

  • Hyun, Jae-Wook;Lee, Seong-Chan;Ihm, Yang-Bin;Kim, Dong-Hwan;Ko, Sang-Wook;Kim, Kwang-Sik
    • The Korean Journal of Pesticide Science
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    • v.5 no.2
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    • pp.37-44
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    • 2001
  • The biological effects of the iminoctadine tris (albesilate) and kresoxim-methyl for the protection of citrus postharvest diseases caused by penicillium spp. were assayed. In vitro tests, $EC_{50}$ values of iminoctadine tris(albesilate) were $0.01{\sim}0.02\;and\;0.01{\mu}g$ a.i./mL against mycelial growth of P. italicum and P. digitatum, respectively, but iminoctadine tris(albesilate) at $0.64{\mu}g$ a.i. /mL inhibited a little mycelial growth of unknown Penicillium sp. which produced another symptom different to blue and green mold caused by P. italicum and P. digitatum, respectively. And against germination and growth of germ tube of P. italicum and P. digitatum, $EC_{50}$ value of iminoctadine tris(albesilate) was $0.0013{\sim}0.0025{\mu}g$ a.i./mL. But spore germination of unknown Penicillium spp. was not nearly inhibited at $0.2{\mu}g$ a.i./mL. $EC_{50}$ values of kresoxim-methyl were $0.08{\sim}0.16$, 0.04 and $0.16{\mu}g$ a.i./mL against mycelial growth of P. italicum, P. digitatum and unknown Penicillium sp., respectively, and $0.04{\sim}0.08{\mu}g$ a.i./mL and $0.01{\sim}0.02{\mu}g$ a.i./mL against germination and growth of germ tube of P. italicum and unknown Penicillium sp., and P. digitatum, respectively. Iminoctadine tris(albesilate) and kresoxim-methyl were markedly effective to control the postharvest disease by 7 days spray prior to harvest. When the fruits were sprayed with iminoctadine-tris(albesilate) ($200{\mu}g$ a.i./mL) and kresoxim-methyl ($155{\mu}g$ a.i./mL) 7 days prior to harvest and subsequently stored for 90 days, the percentage of diseased fruit by Penicillium spp. was $3.6{\pm}1.8%$ in treatment of kresoxim-methyl and $5.9{\pm}1.8%$ in iminoctadine-tris(albesilate), respectively. On the other hand, tile percentage of diseased fruit was relatively high, $20.3{\pm}10.0%\;and\;19.5{\pm}9.6%$ in thiophanate-methyl ($700{\mu}g$ a.i./mL) and non-treatment, respectively. Maximum residue amount (ppm) among fruits (flesh and peel) assayed 0, 30, 60 and 90 days after storage was 0.45 and 0.10 ppm in treatment of kresoxim-methyl and iminoctadine, respectively.

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Comparison of Chestnut (Castanea spp.) Quality Characteristics according to Storage Temperatures and Cultivars (밤 과실의 저장온도 및 품종에 따른 품질 변화 비교)

  • Joo, Sukhyun;Kim, Mahn-Jo;Kim, Mee-Sook;Lee, Uk
    • Journal of Korean Society of Forest Science
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    • v.105 no.1
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    • pp.93-102
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    • 2016
  • This research was conducted for major cultivated chestnut (Castanea spp.) in Korea to compare chestnut quality characteristics according to storage temperatures ($4^{\circ}C$ vs. $-1^{\circ}C$) and cultivars. Color, hardness, soluble solids content (SSC), rate of decay and sensory evaluation were investigated during 16 weeks storage. Cultivars 'Tsukuba' and 'Ishizuchi' showed the least pericarp color change while cultivars 'Riheiguri' and 'Daebo' showed the most pericarp color change during storage. Chestnut fruits stored at $-1^{\circ}C$ showed less pericarp color change than those stored at $4^{\circ}C$. Cultivars 'Arima' and 'Tanzawa' exhibited the decrease tendency of hardness with lowest hardness during storage at $-1^{\circ}C$. Chestnut fruits stored at $4^{\circ}C$ showed high hardness than those stored at $-1^{\circ}C$. Cultivars 'Riheiguri' showed the highest increase of SSC, while cultivar 'Arima' showed the lowest increase of SSC after 16 weeks of storage. The SSC increased in nuts from all cultivars at both temperatures, but nuts stored at $-1^{\circ}C$ showed higher increases in SSC than nuts stored at $4^{\circ}C$. Cultivars 'Ishizuchi' and 'Riheiguri' showed high rates of decayed nuts in contrast to cultivars 'Daebo', 'Okkwang' and 'Tanzawa' that showed low rates of decayed nuts during storage. The chestnut fruit stored at $-1^{\circ}C$ showed less decay than fruit stored at $4^{\circ}C$. Texture and sweetness were tested for sensory evaluation. Among the tested cultivars, 'Riheiguri', 'Daebo' and 'Tsukuba' showed hard texture and very sweetness, while 'Tanzawa' showed relatively soft texture. 'Arima' and 'Okkwang' showed weak sweetness during storage. Nuts stored at $4^{\circ}C$ exhibited harder texture than nuts stored at $-1^{\circ}C$ while $-1^{\circ}C$ exhibited more sweetness than nuts stored at $4^{\circ}C$. Chestnuts for hard texture and short-term storage (less than one month), $4^{\circ}C$ will be a proper storage temperature, while in order to store long-term (more than 4 month), $-1^{\circ}C$ will be a proper storage temperature. Result from this study provide base-line data of postharvest management for Korean cultivated chestnut as well as contributing increased product value and income for chestnut producers.

A study of dietary intake of total sugars by elementary students in Jeju province (제주지역 초등학생의 총당류 섭취실태 조사)

  • Ko, Yang Sook;Kim, Eun Mi;Lee, Hyun Sook
    • Journal of Nutrition and Health
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    • v.48 no.1
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    • pp.81-93
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    • 2015
  • Purpose: The aim of this study was to estimate total sugar intake and sugar intake-related dietary habit and nutrient intake of elementary school students. Methods: Subjects included 701 elementary school students (351 boys and 350 girls) residing in Jeju. The research analyzed a sample and was carried out using the 24 hour recall method and one day consumption of total sugar. Results: The average daily total sugar intake was 55.3 g (male 53.1 g, female 57.6 g). Total sugar intake per meal was 6.3 g (11.4%) from breakfast, 6.9 g (12.5%) from lunch, and 7.2 g (13.0%) from dinner, 34.9 g (63.1%). Major food groups of total sugar were milks (17.04 g), grains and their products (12.79 g), fruits and their products (9.40 g), and sugars and sweets (7.30 g). In analysis of the amount of total sugar consumption from several recipes, much was taken in from dessert and snacks such as dairy products (15.08 g), bread and snacks (6.4 g), beverages (5.5 g), fruits (5.1 g), and ice cakes (4.8 g). Total sugar consumption was high for rices and side dishes such as noodles (5.1 g), stir-fried foods (2.3 g), fried foods (1.4 g), cooked rice with seasoning (1.4 g), and hard-boiled foods (1.1 g). In comparison of total sugar consumption for sugar sources, daily intake of natural sugar, added sugar, and natural and added sugar were 25.8 g, 23.3 g, and 6.2 g, respectively. High sugar intake group, which was over 20% of the energy from total sugar intakes, consumed significantly less Fe, Zn, and niacin than the low sugar intake group, which was below 20%. Conclusion: Total sugar intake of fifth graders of elementary schools on Jeju Island was 55.4 g/day, and it was usually intake from snacks. Therefore, elementary school students need nutritional education for proper selection of better snacks and for reduction of dietary sugar intake.

Quality Evaluation of Minimally Processed Asian Pears (신선편의 식품화된 신고배의 저장 중 이화학적 품질변화)

  • Kim, Gun-Hee;Cho, Sun-Duk;Kim, Dong-Man
    • Korean Journal of Food Science and Technology
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    • v.31 no.6
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    • pp.1523-1528
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    • 1999
  • The consumer's demands for minimally processed fruits and vegetables have been increased rapidly because of its convenient handling, fresh-like quality as well as producing less wastes from the environmental point of view. Asian pears which are one of the main fruits widely produced and consumed in Korea easily lost their characteristics due to browning and softening after cutting. The objective of this study is to investigate the effects of various treatments on delaying deterioration of sliced Asian pears. 'Shingo' pear slices were treated with various solutions $(1%\;NaCl,\;0.2%\;L-cysteine,\;1%\;CaCl_2\;or\;1%\;calcium\;lactate)$ and were packaged with low density polyethylene $(LDPE,\;60\;{\mu}m)$, ceramic $(CE,\;60\;{\mu}m)$ or vacuum $(Ny/PE,\;80\;{\mu}m)$ film at $20^{\circ}C\;and\;0^{\circ}C$. In order to evaluate the quality of packaged sliced pears, quality index was determined in terms of color, firmness, soluble solids, titratable acidity. ascorbic acid, changes of gas composition, microbial test, and sensory quality. The results showed that sliced 'Shingo' pears packaged with CE and vacuum film maintained better quality than with LDPE at $0^{\circ}C\;and\;20^{\circ}C$. To retard browning and softening. 0.2% L-cysteine and 1% NaCl solutions applied for 1 minute were effective to reduce surface browning of sliced pears, and 1% $CaCl_2$ was the most effective to prevent softening.

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Method Development for Determination of Chlorogenic Acid and Arbutin Contents in Fruits by UHPLC-MS/MS (UHPLC-MS/MS를 이용한 과일류 중 클로로젠산 및 알부틴 동시분석법 개발)

  • Choi, Young-Ju;Jeon, Jong-Sup;Kim, Woon-Ho;Jung, You-Jung;Ryu, Ji-Eun;Choi, Jong-Chul;Chae, Kyung-Suk;Lee, Jin-Hee;Do, Young-Sook;Park, Young-Bae;Yoon, Mi-Hye
    • Journal of Food Hygiene and Safety
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    • v.34 no.5
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    • pp.413-420
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    • 2019
  • In this study, a sample preparation method and a simultaneous determination method by ultra-high performance liquid chromatography coupled with tandem mass spectrometry for 9 isomers of chlorogenic acid and arbutin in fruits were developed. The samples were extracted using 90% methanol (pH 3.0), with the solutions being shaken and then sonicated for 10 min each. After centrifugation at 4,000 rpm for 10 min, the extraction was concentrated under a vacuum at $40^{\circ}C$ using a vacuum evaporator. The residue was dissolved in 5 mL of 5% methanol and filtered through a $0.45{\mu}m$ membrane before UHPLC-MS/MS analysis. The separations were performed on a C18 column with gradient elution of water (containing 0.1% formic acid) and methanol (containing 0.1% formic acid). The specificity, linearity, limit of detection, limit of quantification, accuracy, and precision of the proposed methods were also evaluated.

Occurrence and eradication of Plum pox virus on Ornamentals in Korea, 2016-2017 (2016-2017년 국내 핵과류에서의 자두곰보병 발생 및 방제)

  • Kim, Mikyeong;Kim, Gi-Su;Kwak, Hae-Ryun;Kim, Jeong-Eun;Seo, Jang-Kyun;Hong, Seong-Jun;Lee, Gyeong-Jae;Kim, Ju-Hui;Choi, Min-Kyeong;Kim, Byeong-Ryeon;Kim, Ji-Gwang;Han, In-Yeong;Lee, Hyeon-Ju;Won, Heon-Seop;Kang, Hyo-Jung;Han, Jong-Woo;Ko, Suk-Ju;Kim, Hyo-Jeong;Kim, Seung-Han;Lee, Jung-Hywan;Choi, Hong-Soo
    • Research in Plant Disease
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    • v.25 no.1
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    • pp.8-15
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    • 2019
  • Plum pox virus (PPV) is a significant viral disease in Prunus spp. worldwide. A nationwide survey was started in Prunus spp. orchards, since PPV was first detected from peach in Korea, 2015. During 2016-2017, samples were collected from 30,333 trees in 1,985 orchards of stone fruits in 8 provinces and 4 cities, Korea and tested by RT-PCR using specific PPV primer set. As a result, 21 trees including peach (9 trees), Japanese apricot (4 trees), plum (1 tree), apricot (7 trees) in 10 orchards were infected and controlled by eradication program. Amplicons of the expected size (547 bp) were obtained from total RNA of seven peach trees in 2016, and directly sequenced. BLAST analysis revealed the highest nucleotide (NT) identity (99%) with a PPV D isolates (LC331298, LT600782) in Genbank. The seven isolates from shared nt sequence identities of 98 to 100% with one another. Phylogenetic analysis showed the isolates in peach clustered closely with the PPV-D isolates from Korea, Japan, USA, and Canada. This is, to our knowledge, the first report of the presence of PPV in Prunus spp. orchards in Korea, 2016-2017, we hope that our results and efforts will contribute to effective measures for eradication of PPV.

Comparison of Pesticide Resideus in Fruits by Part (과일류의 부위별 잔류농약 함량 비교)

  • Moon, Kyeong-Eun;Park, Myoung-Ki;Bae, Ho-Jeong;Lee, Jung-Hee;Lee, Yu-Na;Kim, Jung-Sun;Kang, Choong-Won;Lee, Jin-Hee;Choi, Ok-Kyung
    • Journal of Food Hygiene and Safety
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    • v.37 no.4
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    • pp.260-270
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    • 2022
  • In this study, we tested for pesticide residues in 114 samples representing 20 different types of fruit circulating in Gyeonggido. A total of 341 pesticides were identified via a multi-residue method using GC-NPD, GC-ECD, GC-MSMS, and LC-MSMS. In whole fruit, pesticides were detected in 48 cases, with a concentration range of 0.0025-0.1663 mg/kg. In the peels, pesticides were detected in 85 cases, with a concentration range of 0.0026-4.7185 mg/kg and a higher average concentration than was found in whole fruit. In the pulp, pesticides were detected in 8 cases, with a concentration range of 0.0021-0.0993 mg/kg. In most of the peels, pesticides were detected below MRL. In the kiwi peel, deltamethrin (with an MRL of 0.05 mg/kg) was detected at 0.1020 mg/kg. Since MRLs are applied to whole fruit, these levels are not unsuitable for ingestion. However, washing is recommended before ingestion to promote healthier and safer inclusion of the peel in the diet. Continuous monitoring of the peel and pulp is indicated.

Study on Nutritional Knowledge and Food Consumption Differences of Middle School Students living in Rural and Urban Areas of Inner Mongolia (중국 내몽고 지역 도시와 농촌 중학생의 영양지식 및 식습관 차이 연구)

  • Li, Ying;Lee, Youngmi;Park, Nari;Park, Haeryun
    • Journal of the East Asian Society of Dietary Life
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    • v.25 no.6
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    • pp.933-941
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    • 2015
  • This study investigated differences in nutritional knowledge and eating habits of adolescents living in rural and urban areas of Inner Mongolia, China. A survey was conducted on 869 middle school students in Hohhot, Inner Mongolia: 436 from urban and 433 from rural school. Subjects answered a questionnaire about socio-economic characteristics such as parents' education level and family affluence scale, nutritional knowledge, needs for nutritional education and food habits. Levels of parents' education (p<0.001) and family affluence (p<0.001) were significantly higher in urban students. Nutritional knowledge level of urban students was higher than that of their counterparts (p<0.001). However, rural students reported higher needs for nutritional education (p<0.001). Rural students more frequently consumed snacks (p<0.001) and instant noodles (p<0.001) than urban students. However, consumption frequencies of lunch (p<0.001), dinner (p<0.001), fast food (p<0.001), fruits (p<0.001), vegetables (p<0.001), and milk (p<0.001) were higher in urban students. Considering differences in eating patterns between urban and rural students in Inner Mongolia, appropriate nutritional education programs and nutritional policies should be established for rural students to promote a healthy diet.

Implementation of Remote Monitoring Scenario using CDMA Short Message Service for Protected Crop Production Environment

  • Bae, Keun-Soo;Chung, Sun-Ok;Kim, Ki-Dae;Hur, Seung-Oh;Kim, Hak-Jin
    • Journal of Biosystems Engineering
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    • v.36 no.4
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    • pp.279-284
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    • 2011
  • Protected vegetable production area is greater than 26% of the total vegetable production area in Korea, and portion of protected production area is increasing for flowers and fruits. To secure stable productivity and profitability, continuous and intensive monitoring and control of protected crop production environment is critical, which is labor- and time-consuming. Failure to maintain proper environmental conditions (e.g., light, temperature, humidity) leads to significant damage to crop growth and quality, therefore farmers should visit or be present close to the production area. To overcome these problems, application of remote monitoring and control of crop production environment has been increasing. Wireless monitoring and control systems have used CDMA, internet, and smart phone communications. Levels of technology adoption are different for farmers' needs for their cropping systems. In this paper, potential of wireless remote monitoring of protected agricultural environment using CDMA SMS text messages was reported. Monitoring variables were outside weather (precipitation, wind direction and velocity, temperature, and humidity), inside ambient condition (temperature, humidity, $CO_2$ level, and light intensity), irrigation status (irrigation flow rate and pressure), and soil condition (volumetric water content and matric potential). Scenarios and data formats for environment monitoring were devised, tested, and compared. Results of this study would provide useful information for adoption of wireless remote monitoring techniques by farmers.

Year-dependent variations of organic acid contents in pre-matured fruits of 175 Korean Prunus mume landraces. (국내 수집 매실유전자원의 청매실 함유 유기산 함량의 연차 변이)

  • Gwak, Bo-Kyeong;Eom, Soyeong;Wimonmuang, Kanphassorn;Kang, Hee-Kyoung;Lee, Young-Sang
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2018.10a
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    • pp.115-115
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    • 2018
  • 매실나무(Prunus mume)의 풋과일인 매실은 피로회복, 소화촉진, 살균 및 항균작용 등 다양한 생리활성을 갖고 있다. 본 연구는 국내 수집 매실 유전자원 172점의 핵심 기능성 물질인 유기산의 함량 특성을 평가하고자 2017년과 2018년 2년간 청매실을 수확하여 옥살산(OA), 사과산(MA), 구연산(CA)의 함량을 HPLC를 이용하여 분석하고 그 연차 변이를 평가하였다. 전체 유전자원 매실의 2년간 총유기산(TA) 함량은 평균 5.5% (최소 3.9%-최대 7.8%)이었으며, MA와 CA 및 OA의 평균 함량은 각각 2.7%, 2.8%, 0.1%였다. 대상 자원 내 유기산별 함량 변이는 CA (RSD=30%)가 MA (RSD=21%)보다 높게 나타났다. 2018년산 매실은 2017년산에 비교할 때 MA와 OA는 함량이 낮아 연차 변이가 인정되었으나 CA는 차이가 없었다. 비록 연차 변이는 있었으나 유전자원별 함량은 MA와 CA 및 TA에서 모두 2017년산과 2018년산간 정의 상관이 인정되어 유기산 함량이 높고 낮은 자원의 특성은 유지됨을 알 수 있었다. 대상 유전자원 중 TA함량이 가장 높았던 자원은 구산매, 용흥흥매, 상금백옥매 등이었으며, 함량이 낮은 자원은 한산매, 지곡매 등이었다. 상기 결과를 통해 매실 유기산 함량관련 국내 매실 유전자원의 유전적 다양성과 연차 변이의 영향을 평가할 수 있었다.

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