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Antifungal Activity or Coptis japonica Root-stem extract and Identification of Antifungal Substances (황련추출액의 항균활성과 항균성물질의 동정)

    • Korean Journal of Plant Resources
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    • v.12 no.4
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    • pp.260-268
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    • 1999
  • Crude extract of Coptis japonica root-stem was evaluated for antifungal activity against Phytophthora capsici, Fusarium oxysporum, Colletotrichum dematium, Colletotrichum truncatum, Botrytis cinerea, Botryosphaeria dothidea and Alternaria porri, and antifungal active compound from the extract was identified. In addition, the usefulness of the extract for some plants disease control was investigated. Crude extract of C. japonica root-stem exhibited antifungal activity against P. capsici, F. oxysporum, C. dematium, B. cinerea, B. dothidea and A. porri. Antifungal activity of the substance isolated from C. japonica root-stem was similar to a standard chemical berberine-Cl. Red-pepper fruit rot, sesame stem rot and welsh-onion alternaria leaf spot were effectively controlled by the crude extract of C. japonica root-stem. Phytotoxicity was not observed in the red-pepper and welsh-onion leaves and red-pepper and strawberry fruits with exogenous foliage application of the crude extract. Seeds germination and radicle growth of red-pepper and sesame were inhibited by the crude extract of C. japonica root-stem. 4.24g of yellowish compound per 100g of C. japonica root-stem was obtained. The compound was identified as berberine-Cl by HPLC.

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Reactive Oxygen Species and Antioxidant Enzyme Activities in Accordance with the Cytotoxicity of Farnesol Against HL-60 Cells (Farnesol의 HL-60 세포에 대한 세포독성과 활성산소 및 항산화효소 활성 변화)

  • Lim, So-Yoon;Park, Sie-Won
    • YAKHAK HOEJI
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    • v.50 no.6
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    • pp.372-380
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    • 2006
  • Farnesol in fruits, vegetables, herbs and leaves acts as bioactive component related with prevention of cancer and psychological malaise. We investigated the cytotoxic effects of farnesol on human leukemic cell, HL-60 cells, by MTT assay using 3- (4,5-Oirnethylthiazol-2-yl) -2,5-diphenyltetrazoliumbromide. Farnesol (0.1${\sim}$50 ${\mu}$g/ml) exhibited cytotoxicities against HL-60 cells in concentration and culture period dependent manner, In the cytotoxic condition induced by farnesol against HL-60 cells, the generation of reactive oxygen species such as O$_2$ and H$_2$O$_2$ were found to be considerably increased. The most prominent augmentations of O$_2$ and H$_2$O$_2$ were over five folds of controls. In an attempt to explore the response of HL-60 cells to the increased O$_2$ and H$_2$O$_2$, superoxide dismutase (SOD), glutathione peroxidase (GPx) and catalase activities of HL-60 cells treated with farnesol were measured. SOD and GPx activities were found to be remarkably elevated by addition of farnesol showing the best results of 273% and 167% of controls, respectively: All data suggest that farnesol may have played as an apoptosis inducer in HL-60 cells via production of reactive oxygen species (ROS) and HL-60 cells may have failed to overcome the damage of ROS on account of still defcient ROS scavengers including SOD and GPx.

Carbamoyl Phosphate Synthase Subunit CgCPS1 Is Necessary for Virulence and to Regulate Stress Tolerance in Colletotrichum gloeosporioides

  • Mushtaq, Aamar;Tariq, Muhammad;Ahmed, Maqsood;Zhou, Zongshan;Ali, Imran;Mahmood, Raja Tahir
    • The Plant Pathology Journal
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    • v.37 no.3
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    • pp.232-242
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    • 2021
  • Glomerella leaf spot (GLS) is a severe infectious disease of apple whose infective area is growing gradually and thus poses a huge economic threat to the world. Different species of Colletotrichum including Colletotrichum gloeosporioides are responsible for GLS. For efficient GLS control, it is important to understand the mechanism by which the cruciferous crops and C. gloeosporioides interact. Arginine is among one of the several types of amino acids, which plays crucial role in biochemical and physiological functions of fungi. The arginine biosynthesis pathway involved in virulence among plant pathogenic fungi is poorly understood. In this study, CgCPS1 gene encoding carbamoyl phosphate synthase involved in arginine biosynthesis has been identified and inactivated experimentally. To assess the effects of CgCPS1, we knocked out CgCPS1 in C. gloeosporioides and evaluated its effects on virulence and stress tolerance. The results showed that deletion of CgCPS1 resulted in loss of pathogenicity. The ∆cgcps1 mutants showed slow growth rate, defects in appressorium formation and failed to develop lesions on apple leaves and fruits leading to loss of virulence while complementation strain (CgCPS1-C) fully restored its pathogenicity. Furthermore, mutant strains showed extreme sensitivity to high osmotic stress displaying that CgCPS1 plays a vital role in stress response. These findings suggest that CgCPS1 is major factor that mediates pathogenicity in C. gloeosporioides by encoding carbamoyl phosphate that is involved in arginine biosynthesis and conferring virulence in C. gloeosporioides.

Color Characteristics of Silk Fabrics Dyed with Natural Red Dyes Without a Mordant (적색계 천연염료를 이용한 견직물 무매염 염색의 염색성)

  • Kim, Hyo-Jin;Lee, Joo-Hyeon
    • Journal of Digital Convergence
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    • v.16 no.12
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    • pp.593-602
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    • 2018
  • The purpose of this study was to investigate the coloring properties of non-mordant dyes by examining local literature on silk fabric dyeing using red-colored natural dyes. Natural dyes can be prepared from the following 8 materials: purple-fleshed sweet potato, Impatiens balsamina, mulberry, fruits of Ligustrum japonicum Thunb, guava leaves, dansam, hibiscus flowers, and pruned branches of Prunus persica. To examine the surface color calculated $L^*$, $a^*$, $b^*$, ${\Delta}E^*$, and K/S value and H V/C. The variables considered in the dyeing experiment were dye concentration, dyeing temperature, dyeing time, and number of dyeing iterations, which were varied to evaluate the dyeing properties and color characteristics. As a result, the abovementioned variables, dye affinity, and red color expression were directly proportional to one another. In this study, it was found that red coloring can be obtained with natural dyes; moreover, excellent dyeing was achieved without the need for repeated dyeing or mordanting processes, which cause environmental pollution.

A Taxonomic Review and Nomenclature of the Opuntia ficus-indica (L). Mill. in Jeju Island (제주도 민속식물인 선인장(Opuntia ficus-indica (L.) Mill.)의 분류학적 검토 후 명명)

  • Yang, Young-Soo;Choi, Byoung-Ki;Oh, Hong-Shik
    • Journal of the Korean Institute of Traditional Landscape Architecture
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    • v.38 no.4
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    • pp.68-73
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    • 2020
  • The purpose of this study is to classify species and correct misidentification of cacti (Opuntia) in Jeju area through morphological characteristics comparison. As a result of the study, it was found that Opuntia ficus-indica and O. ficus-indica var. saboten, which has been reported to grow naturally in Jeju Island, is Opuntia stricta. In the morphological comparison of species, it was confirmed that O. stricta showed clear differences in plant size, cladode shape, color of flowers and fruits, and characteristics of areole compared to the existing reported species. Based on the research results, the scientific name of Jeju cactus was corrected as Opuntia stricta, and the Korean name 'Hae-an-sun-in-jang' was newly named. Furthermore, it was suggested that various research literatures and national biodiversity management organizations need to confirm and correct the misidentification of species.

Clean Label Meat Technology: Pre-Converted Nitrite as a Natural Curing

  • Yong, Hae In;Kim, Tae-Kyung;Choi, Hee-Don;Jang, Hae Won;Jung, Samooel;Choi, Yun-Sang
    • Food Science of Animal Resources
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    • v.41 no.2
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    • pp.173-184
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    • 2021
  • Clean labeling is emerging as an important issue in the food industry, particularly for meat products that contain many food additives. Among synthetic additives, nitrite is the most important additive in the meat processing industry and is related to the development of cured color and flavor, inhibition of oxidation, and control of microbial growth in processed meat products. As an alternative to synthetic nitrite, preconverted nitrite from natural microorganisms has been investigated, and the applications of pre-converted nitrite have been reported. Natural nitrate sources mainly include fruits and vegetables with high nitrate content. Celery juice or powder form have been used widely in various studies. Many types of commercial starter cultures have been developed. S. carnosus is used as a critical nitrate reducing microorganism and lactic acid bacteria or other Staphylococcus species also were used. Pre-converted nitrite has also been compared with synthetic nitrite and studies have been aimed at improving utilization by exploiting the strengths (positive consumer attitude and decreased residual nitrite content) and limiting the weaknesses (remained carcinogenic risk) of pre-converted nitrite. Moreover, as concerns regarding the use of synthetic nitrites increased, research was conducted to meet consumer demands for the use of natural nitrite from raw materials. In this report, we review and discuss various studies in which synthetic nitrite was replaced with natural materials and evaluate pre-converted nitrite technology as a natural curing approach from a clean label perspective in the manufacturing of processed meat products.

Analyzing Growth Reactions of Herbaceous Plants for Irrigation Management

  • Jeong, Myeong Il;Jeong, Na Ra;Han, Seung Won;Kim, Jae Soon
    • Journal of People, Plants, and Environment
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    • v.23 no.3
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    • pp.255-265
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    • 2020
  • Background and objective: The purpose of this study was to provide guidelines for irrigation management by analyzing the effects of soil moisture on the growth characteristics of herbaceous plants in green infrastructure. Methods: In a rain shelter greenhouse, the growth performance of nine species of experimental plants was assessed under different soil moisture contents (20%, 15%, 10%, 5%, and 1%) for about 5 months to analyze plant growth characteristics due to soil humidity. Methods to determine plant growth conditions include surveying growth conditions of the crowns, stems, leaves, flowers and fruits on the aerial part and surveying growth conditions of the roots in the underground part. Results: The results showed that Mukdenia rossii and Astilbe rubra grew well at 15% moisture content with irrigation intervals of 10 and 13 days, respectively. Soil moisture content of 10% with irrigation intervals of 13 and 17 days was appropriate for Sedum kamtschaticum and Pachysandra terminalis. Similarly, Aquilegia japonica and Liriope platyphylla grew well at 15% moisture content with irrigation intervals of 10 and 17 days. However, Ligularia stenocephala grew well-developed stems and roots at 1% soil moisture content and an irrigation interval of 25 days, while the optimum conditions for Lythrum anceps were 5% moisture content and an irrigation interval of 8 days. Conclusion: Although a limited number of experimental plants were used in this study, this study could propose an appropriate irrigation cycle for planting on artificial soil substrates. Based on these results, it is possible to plan suitable planting designs considered irrigation cycles.

Intestinal parasitic infections among children aged 12-59 months in Nyamasheke District, Rwanda

  • Evariste Hakizimana;Ju Yeong Kim;Singeun Oh;Moonsoo Yoon;Tai-Soon Yong
    • Parasites, Hosts and Diseases
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    • v.61 no.3
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    • pp.304-309
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    • 2023
  • Intestinal parasitic infections are a public health burden and a major cause of illness in developing countries. The diseases lead to various health threats, including growth retardation and mental health-related disorders, especially in children. We assessed the risk factors for intestinal parasitic infections among children aged 12-59 months residing in Nyamasheke District, Rwanda. A cross-sectional descriptive study was conducted using secondary data from 1,048 children aged 12-59 months whose stool samples were examined for the presence of intestinal parasites and whose results were registered in the laboratory information system in 2020. The prevalence of intestinal parasites in children aged 12-59 months was 53.2%. The dominant parasites were Ascaris lumbricoides (13.1%), followed by Giardia lamblia (10.9%), Entamoeba histolytica (7.9%), Trichuris trichiura (6.5%), hookworms (1.7%), and Taenia species (1.4%). A significant association was observed between intestinal parasites and the literacy of mothers or children's caregivers (odds ratio (OR)=5.09, P<0.001). Children from farming households were 2.8-fold more likely to contract intestinal parasitic infections than those from nonfarming households (OR=2.8, P<0.001). A significant association was also observed between intestinal parasites and food safety (OR=4.9, P<0.001). Intestinal parasitic infections were significantly associated with hand hygiene practices after using the toilet and washing fresh fruits before eating (P<0.001). The information gathered will help public health providers and partners develop control plans in highly endemic areas in Rwanda.

Effects of Developed Grape Bag on the Physiological Disorders, Pathogenic Decay and Fruit Quality in 'Campbell Early' Grapevines (개발된 포도 봉지 괘대가 '캠벨얼리' 과실의 생리장해와 병 발생 및 품질에 미치는 영향)

  • Lee, Y.C.;Moon, B.W.;Kim, M.S.
    • Journal of Practical Agriculture & Fisheries Research
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    • v.6 no.1
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    • pp.81-89
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    • 2004
  • The effects of developed grape bags on the micro-climate changes of bag, physiological disorder, pathogenic decay, quality and harvest time evaluation in 'Campbell Early' grapevines were studied. The temperature and light transmittance of developed grape bags showed no differences compared with the onces of conventional bag and non-bagging, but relative humidity and the amount of water evaporation were changed in all treatments. The occurrence of unfertilized fruit, poorly colored fruit, russet and gray mold rot showed no significant difference in all treatment at harvest time. Developed grape bags decreased effectively the occurrence of cracking fruit and bitter rot in 'Campbell Early' fruit. There was no difference in growth of cluster and berry, soluble solids and total acidity in fruits, degree of skin color and bloom appearance at harvest time. The skin color and fruit boom and harvest time evaluation in developed grape bags were resulted excellent compared with the once of conventional bag and non bagging.

Avocado Classification and Shipping Prediction System based on Transfer Learning Model for Rational Pricing (합리적 가격결정을 위한 전이학습모델기반 아보카도 분류 및 출하 예측 시스템)

  • Seong-Un Yu;Seung-Min Park
    • The Journal of the Korea institute of electronic communication sciences
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    • v.18 no.2
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    • pp.329-335
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    • 2023
  • Avocado, a superfood selected by Time magazine and one of the late ripening fruits, is one of the foods with a big difference between local prices and domestic distribution prices. If this sorting process of avocados is automated, it will be possible to lower prices by reducing labor costs in various fields. In this paper, we aim to create an optimal classification model by creating an avocado dataset through crawling and using a number of deep learning-based transfer learning models. Experiments were conducted by directly substituting a deep learning-based transfer learning model from a dataset separated from the produced dataset and fine-tuning the hyperparameters of the model. When an avocado image is input, the model classifies the ripeness of the avocado with an accuracy of over 99%, and proposes a dataset and algorithm that can reduce manpower and increase accuracy in avocado production and distribution households.