• 한국생물공학회:학술대회논문집
• /
• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
• /
• pp.291-294
• /
• 2000

The production of polyhydroxybutrate(PHB) by Alcaligenes eutrophus NCIB 11599 was studied in a synthetic medium from whey as a sole carbon source. Especially pH-effect was treated and compared in this study. At the end of fermentation (A) unadjusted to pH, the dry cell weight, PHB concentration, and PHB conversion rate were 10.3g/L, 3.1g/L, and 30%, respectively. At the end of fermentation (B) adjusted to $pH(7.0{\sim}7.5)$, the dry cell weight, PHB concentration, and PHB conversion rate were 12.5g/L, 4.8g/L, and 41%, respectively. PHB conversion rate was about 10% higher on the fermentation (B) than on the fermentation (A).

• Bulletin of the Korean Chemical Society
• /
• 제21권6호
• /
• pp.557-561
• /
• 2000

Various enaryloxynitriles-terminated reactive polymer precursors containing rigid aromatic units were prepared from various diamines and 1-(p-formylphenyl)-1-phenyl-2,2-dicyanoethene (1). Arylate end-capped model compounds linked with azomethine bond were also prepared by reacting p-formylphenyl benzoate with diamines to compare the curing ability. The oligomers were highly soluble in polar aprotic solvents such as N,N-dimethylformamide, dimethylsulfoxide and N-methyl-2 -pyrrolidinone. They generally showed an exothermic curing process between $280-350^{\circ}C$, attributable to the thermal crosslinking of the dicyanovinyl group in DSC analysis, and no weight loss at curing temperature. Upon heating the polymer precursors, heat-resistant and insoluble network polymers were obtained. Thermogravimetric analyses of the precursors containing rigid aromatic units showed thermal stability with a 77-92% residual weight at $500^{\circ}C$ under nitrogen.

• 한국기계가공학회지
• /
• 제19권4호
• /
• pp.9-15
• /
• 2020

Carbon fiber reinforced plastic (CFRP) is a composite material useful in the aerospace and automotive industries because of its light weight and high strength. In this study, side milling tests were carried out using AlCrN, diamond-like carbon (DLC), and diamond-coated end mills. Additionally, a comparison study according to the cobalt content was conducted. Thus, tool wear and surface quality were examined and the influences of using coating and a certain material type were analyzed. The surface roughness of the machined surface was measured. Microscope observations revealed that the CFRP fiber at the machined surface was not damaged even at a cutting distance of 3,000 mm. Therefore, this study showed that the diamond-coated end mill containing 6% cobalt is appropriate for milling CFRP.

• 한국철도학회:학술대회논문집
• /
• 한국철도학회 2007년도 춘계학술대회 논문집
• /
• pp.155-161
• /
• 2007

A bogie frame is typical safty part of railway vehicle. Main performance of a bogie frame is to support a car body and passenger weight and transfer traction and braking force. This paper is to evaluate the static strength and fatigue strength for the end trailer bogie with UIC 615-4 code. Therefore stress analysis of the end trailer bogie frame has been performed for various loading condition according to the UIC 615-4 code and haigh diagram is used for evaluation of fatigue strength. By the results of these analysis, static and fatigue strength of the end trailer bogie is satisfied with a applied criterion.

• 제어로봇시스템학회논문지
• /
• 제15권7호
• /
• pp.728-733
• /
• 2009

This paper presents the compensation of mechanical deflection error in SCARA robot. End of robot gripper is deflected by weight of arm and pay-load. If end of robot gripper is deflected constantly regardless of robot configuration, it is not necessary to consider above mechanical deflection error. However, deflection in end of gripper varies because that moment of each axis varies when robot moves, it affects the relative accuracy. I propose the compensation method of deflection error using neural network. FEM analysis to obtain the deflection of gripper end was carried out on various joint angle, the results is used in neural network teaming. The result by simulation showed that maximum relative accuracy reduced maximum 9.48% on a given working area.

• 한국철도학회:학술대회논문집
• /
• 한국철도학회 2003년도 추계학술대회 논문집(III)
• /
• pp.103-108
• /
• 2003

Among welded structure bogies in use for high speed freight car, a part of bogies manufactured in 1999 and 2000 have found problems that failure occurs in its end beam. In case of a freight car a difference of weight between empty and loading conditions are worse than in case of a passenger car. Moreover its brake system is tread brake without second suspension system. A failure of end beam is supposed to be due to loading by brake rather than vertical loading by freight. This failure can make brake system useless and may be a cause of derailment in the worst case. In this study, in order to be proved the cause of failure and to solve the problem it is described that we have measured load history acted on end beam and set up the procedures.

• 한국국방경영분석학회지
• /
• 제3권1호
• /
• pp.83-96
• /
• 1977

The combustion chamber and nozzle of an end burning, small spherical rocket is designed. A spherical external shape has a number of advantages such as fixed center-of-gravity and minimum aerodynamic precession torques during flight and a better mass distribution for gyro-stabilization as contrasted to a conventional ogive rocket shape. It is shown that the cross-sectional variation of the end burning solid propellant with length is an exponential geometry to provide a constant thrust-weight ratio of the rocket device during the propellant burning period, and that the factors which affect the attainment of the constant relationship of thrust to weight in the design are the initial propellant area, initial weight of the rocket and propellant density. The measurement of the transient thrust in the ground static test using black powder propellant supports the predicted results. A wind tunnel having a $30{\times}30{\times}75cm$ test section and Mach number 0.11 is constructed, and a simple balance-type device is designed for the measurement of the drag of a spinning sphere. The experimental results indicate that the. spinning has no effect on the magnitude of the drag up to the Reynolds number $3{\times}10^5$. Numerical computation of the flight trajectories for various launching angles is presented, and the gyro-stabilization of spinning sphere is discussed.

• 한국환경복원기술학회지
• /
• 제14권3호
• /
• pp.57-69
• /
• 2011

This study examined the relationship between Phragmites australis' growth and sediment properties at mud tidal flat of Donggum-ri, Gilsang-myeon, Gangwha-gun, Incheon city. Field survey was carried out from May, 2010 to October, 2010. Water content, soil texture, electric conductivity and water table depth for sediment, density, height, dry weight and flowering for P. australis were examined at several plots from the starting point (the coastal embankment) to the end point of the two populations. The result was as follows. Firstly, the water table increased along distance from the embankment at one line (N-line) but was similar at the other line (S-line) in a P. asustralis population. Water tables were higher out of than within a P. australis population at two populations. Secondary, in N-line, the height and dry weight of P. australis decreased along the distance from embankment but, in S-line, those were similar in its population. P. australis' growth was dependent on electric conductivity at lower layer (water table level) rather than upper one (the surface). Thirdly, density of P. australis changed during growing season and was similar in a population, except for the end point of patch. In summary, the growth and distribution of P. australis were dependent on salt content of tidal flat's sediment (water table level) and this was affected by fresh water of the inland.

• BMB Reports
• /
• 제28권4호
• /
• pp.281-289
• /
• 1995

Homogeneous human deoxycytidine kinase was purified in one step from a variety of spontaneous human leukemic cells (T-ALL, B-ALL, B-CLL, AML, CML), and from cultured T-lymphoblast cells (MOLT-4) using the newly developed affinity medium, $dCp_4$-Sepharose. Starting with an ammonium sulfate fraction, purification was achieved in one step with the kinase being eluted from a column by the end product inhibitor, dCTP. The purified deoxycytidine kinase from T-ALL cells phosphorylated deoxyadenosine and deoxyguanosine, as well as deoxycytidine. The enzyme purified from T-ALL and B-CLL cells yielded one major band with a molecular weight of 52 kDa determined by SDS-polyacrylamide gel electrophoresis. AML and CML cells yielded one 52 kDa band and an extra band of 30 kDa molecular weight. On the other hand, B-ALL and MOLT-4 cells showed a low molecular weight band of 30 kDa only. However, the electrophoretic mobilities of enzymatic activity in 12% non-denaturing gels were identical for the dCyd kinase from all different kinds of leukemic cell lines, except that the B-ALL, B-CLL, and MOLT-4 cell preparations had an extra minor peak, all at the same position. dAdo and dCyd phosphorylating activities comigrated indicating that these activities are all associated with the same protein. Two new methods, a disk implantation method and a nitrocellulose powder method were used with a small amount of enzyme protein to raise polyclonal antibodies against dCyd kinase purified from T-ALL cells.

• 한국유변학회:학술대회논문집
• /
• 한국유변학회 2000년도 춘계 학술발표회 논문집
• /
• pp.104-107
• /
• 2000