• Asian Pacific Journal of Cancer Prevention
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• 제14권1호
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• pp.387-392
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• 2013

Objective: The aim of the present study was to explore mechanisms by which let-7c suppresses NSCLC cell proliferation. Methods: The expression level of let-7c was quantified by qRT-PCR. A549 and H1299 cells were transfected with let-7c mimics to restore the expression of let-7c. The effects of let-7c were then assessed by cell proliferation, colony formation and cell cycle assay. Mouse experiments were used to confirm the effect of let-7c on tumorigenicity in vivo. Luciferase reporter assays and Western blotting were performed to identify target genes for let-7c. Results: HOXA1 was identified as a novel target of let-7c. MTS, colony formation and flow cytometry assays demonstrated that forced expression of let-7c inhibited NSCLC cell proliferation by inducing G1 arrest in vitro, consistent with inhibitory effects induced by knockdown of HOXA1. Mouse experiments demonstrated that let-7c expression suppressed tumorigenesis. Furthermore, we found that let-7c could regulate the expression of HOXA1 downstream effectors CCND1, CDC25A and CDK2. Conclusions: Collectively, these results demonstrate let-7c inhibits NSCLC cell proliferation and tumorigenesis by partial direct targeting of the HOXA1 pathway, which suggests that restoration of let-7c expression may thus offer a potential therapeutic intervention strategy for NSCLC.

• Asian Pacific Journal of Cancer Prevention
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• 제15권9호
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• pp.3955-3958
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• 2014

Background: The melastatin-related transient receptor potential 7 channel (TRPM7) is a nonselective cation channel that has been shown to promote tumor metastasis and progression. In this study, we determined the expression of TRPM7 in ovarian carcinomas and investigated its possible prognostic value. Materials and Methods: Samples were collected from 138 patients with ovarian cancer. Expression of TRPM7 was assessed by real-time PCR and immunohistochemistry, expressed with reference to an established scoring system and related to clinical pathological factors. Kaplan-Meier survival analysis was applied to estimate disease-free survival (DFS) and overall survival (OS). Univariate and multivariate cox regression analyses were performed to correlate TRPM7 expression levels with DFS and OS. Results: TRPM7 was highly expressed in ovarian carcinoma and significantly associated with decreased disease-free survival (DFS: median 20 months vs. 42 months, P=0.0002) and overall survival (OS: median 27 months vs. 46 months, P<0.001). Conclusion: Overexpression of TRPM7 expression is significantly associated with poor prognosis in patients with ovarian cancer.

• Asian Pacific Journal of Cancer Prevention
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• 제13권5호
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• pp.2355-2362
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• 2012

The SH2B1 adaptor protein is recruited to multiple ligand-activated receptor tyrosine kinases that play important role in the physiologic and pathologic features of many cancers. The purpose of this study was to assess SH2B1 expression and to explore its contribution to the non-small cell lung cancer (NSCLC). Methods: SH2B1 expression in 114 primary NSCLC tissue specimens was analyzed by immunohistochemistry and correlated with clinicopathological parameters and patients' outcome. Additionally, 15 paired NSCLC background tissues, 5 NSCLC cell lines and a normal HBE cell line were evaluated for SH2B1 expression by RT-PCR and immunoblotting, immunofluorescence being applied for the cell lines. Results: SH2B1 was found to be overexpressed in NSCLC tissues and NSCLC cell lines. More importantly, high SH2B1 expression was significantly associated with tumor grade, tumor size, clinical stage, lymph node metastasis, and recurrence respectively. Survival analysis demonstrated that patients with high SH2B1 expression had both poorer disease-free survival and overall survival than other patients. Multivariate Cox regression analysis revealed that SH2B1 overexpression was an independent prognostic factor for patients with NSCLC. Conclusions: Our findings suggest that the SH2B1 protein may contribute to the malignant progression of NSCLC and could offer a novel prognostic indicator for patients with NSCLC.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.6527-6531
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• 2012

Purpose: Complement receptor 1 (CR1) is induced by Epstein-Barr virus (EBV) and may be a potential biomarker of nasopharyngeal carcinoma (NPC). We conducted the present study to evaluate the association of CR1 expression with clinicopathological features and prognosis of NPC. Methods: We enrolled 145 NPC patients and 110 controls. Expression levels of CR1 in peripheral blood mononuclear cells (PBMCs) were detected using quantitative real-time PCR and associations with clinicopathological features and prognosis were examined. Results: CR1 levels in the NPC group [3.54 (3.34, 3.79)] were slightly higher than those in the controls [3.33 (3.20, 3.47)] (P<0.001). Increased CR1 expression was associated with histology classification (type III vs. type II, P=0.002), advanced clinical stage (P=0.003), high T stage (P=0.017), and poor overall survival (HR, 4.89; 95% CI, 1.23-19.42; P=0.024). However, there were no statistically significant differences in CR1 expression among N or M stages. Conclusion: These findings indicate that CR1 expression in PBMCs may be a new biomarker for prognosis of NPC and a potential therapeutic target.

• Asian Pacific Journal of Cancer Prevention
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• 제14권11호
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• pp.6347-6350
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• 2013

Post-transplant lymphoproliferative disorder (PTLD) is a common complication of therapeutic immunosuppression after organ transplantation. Gene expression profile facilitates the identification of biological difference between Epstein-Barr virus (EBV) positive and negative PTLDs. Previous studies mainly implemented variance/regression analysis without considering unaccounted array specific factors. The aim of this study is to investigate the gene expression difference between EBV positive and negative PTLDs through partial least squares (PLS) based analysis. With a microarray data set from the Gene Expression Omnibus database, we performed PLS based analysis. We acquired 1188 differentially expressed genes. Pathway and Gene Ontology enrichment analysis identified significantly over-representation of dysregulated genes in immune response and cancer related biological processes. Network analysis identified three hub genes with degrees higher than 15, including CREBBP, ATXN1, and PML. Proteins encoded by CREBBP and PML have been reported to be interact with EBV before. Our findings shed light on expression distinction of EBV positive and negative PTLDs with the hope to offer theoretical support for future therapeutic study.

• 대기
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• 제18권4호
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• pp.279-286
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• 2008

In the tropical western Pacific (especially, west of 135oE, including South China Sea and the Philippines), during boreal summer, it was found that a strong correlation exists between the tropical cyclone activity and the drought conditions in Korea. During the summer drought, firstly, there were strong ascending flows over the tropical western Pacific with more tropical cyclone genesis, and to compensate for this, descending flows develop in the mid-latitudes, thereby causing drought; in other words, a secondary circulation is formed between the tropical western Pacific and mid-latitudes of East Asia. Secondly, the developments of both the subtropical western Pacific high and the Manchurian low are suppressed. As a result, both the land-sea pressure gradient and the southerly flow from low-latitudes to Korean area are also weakened, which reduces approaches of tropical cyclones to this area despite the high frequency of their geneses.

• 자원환경지질
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• 제7권1호
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• pp.1-21
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• 1974

From the point of view of geological history, the land of South Korea is regarded as the subject of processes of the changes in formations of several geological blocks such as Kyonggi massif, Yeongnam massif, Taebaegsan basin, Kyungsang basin and so on. Through the long period of geological chronology, the present topography and geotectonics have been formed by the complicate interactions of epirogenetic movements, magmatism, orogenesis, differential vertical movements, metamorphism and sedimentation. The reason of the crust movements mentioned above, is suppossed that the Pacific and West Pacific plate have subducted directly or indirectly into the East Asia plate. This fact can be endorsed by the results of the studies on the heat flow, gravity anomaly, absolute age dating, tectonic lineation, lithofacies and the temperature of hot spring in South Korea. The formations of metal ore deposits as well as other geological processes can be determined by the mechanical control of the plates and be divided into several systematic patterns. The investigation of about 110 metal mines in South Korea shows the following results. (1) Plate boundary volcanic type is about 28% (2) Plate boundary plutonic type is about 44% (3) Intraplate sedimentary type is about 26% (4) Intraplate magmatic type is about 2%.

• Asian Pacific Journal of Cancer Prevention
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• 제14권8호
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• pp.4865-4869
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• 2013

Background: The objectives of this present study were to assess the awareness of cervical cancer and its risk factors among female undergraduates in South Africa, and to determine the their level of acceptability of Human Papillomavirus (HPV) vaccination. Materials and Methods: This cross-sectional study was conducted in March 2013 among 440 full time undergraduate female students. Results: Of those students who never had sex (n=163), 96 (58.9%) had ever heard of cervical cancer and only 12 students (12.5%) knew that HPV causes cervical cancer. More than a third (35.4%) of the students correctly said that sexual intercourse before age of 18 years is a risk factor for cervical cancer and 55.2% of the students knew about the Pap smear test which is used for screening cervical cancer. The majority (77.3%) were willing to accept HPV vaccination. Results revealed that students who knew about the Pap smear test, and were aware that having multiple sex partners, sexual intercourse before the age of 18 years, smoking and having contracted any STDs are risk factors for cervical cancer were more likely to accept HPV vaccination compared to other groups. Conclusions: The general knowledge of South African female university students about cervical cancer is not sufficient but they have positive attitudes toward getting vaccinated with the HPV vaccine.

• 대한지리학회지
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• 제48권2호
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• pp.184-203
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• 2013

이 연구에서는 장기간의 관측 자료를 이용하여 적도 저주파 진동과 관련된 한반도 여름철 강수의 변동성을 분석하였다. EOF 분석을 실시한 결과 여름철 대표적인 한반도 강수 패턴은 남한과 북한이 반대의 위상을 가지며 1990년대 중반을 기점으로 레짐 이동이 나타나는 변동 특성을 보였다. 한반도의 여름철 강수는 엘니뇨/라니냐 변동에 따라 적도 동태평양에서 해수면 온도가 증가하는 강한 엘니뇨 해와 적도에서부터 중위도 서태평양까지 남북으로 연결되어 강수량이 증가하는 약한 라니냐 해에 특히 우리나라(남한)의 남부지방에서 증가하는 경향을 보여주었다. 여름철 인도, 북서태평양, 북동아시아 몬순 지수 및 여름철 강수지수에 대한 계절 내 변동에 의해, 우리나라 강수는 6월 인도 몬순 지수와 양의 상관이 있고 7월 북서태평양 몬순 지수와 음의 상관이 있으며 8월 인도 몬순 지수와 가장 높은 음의 상관이 있는 것으로 나타났다.

• 한국해안해양공학회:학술대회논문집
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• 한국해안해양공학회 1995년도 정기학술강연회 발표논문 초록집
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• pp.13-15
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• 1995

The horizontal and vertical circulations are considered in the South China Sea, based on the 80 years' winds (COADS), 10 years' XBTs (NODC), and about 10 years' sea-level data at Kaoshiung, Taiwan and Singapore. The South China is largest marginal sea in the western North Pacific, which is predominantly governed by Southeast Asian Monsoons. (omitted)