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Effect of Anchorage Number on Behavior of Reinforced Concrete Beams Strengthened with Glass Fiber Plates

  • Kaya, Mustafa;Kankal, Zeynel Cagdas
    • International Journal of Concrete Structures and Materials
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    • v.9 no.4
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    • pp.415-425
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    • 2015
  • Reinforced concrete beams with insufficient shear reinforcement were strengthened using glass fiber reinforced polymer (GFRP) plates. In the study, the effect of the number of bolts on the load capacity, energy dissipation, and stiffness of reinforced concrete beams were investigated by using anchor bolt of different numbers. Three strengthened with GFRP specimens, one flexural reference specimen designed in accordance to Regulation on Buildings Constructed in Disaster Areas rules, and one shear reinforcement insufficient reference specimen was tested. Anchorage was made on the surfaces of the beams in strengthened specimens using 2, 3 and 4 bolts respectively. All beams were tested under monotonic loads. Results obtained from the tests of strengthened concrete beams were compared with the result of good flexural reference specimen. The beam in which 4 bolts were used in adhering GFRP plates on beam surfaces carried approximately equal loads with the beam named as a flexural reference. The amount of energy dissipated by strengthened DE5 specimen was 96 % of the amount of energy dissipated by DE1 reference specimen. Strengthened DE5 specimen initial stiffness equal to DE1 reference specimen initial stiffness, but strengthened DE5 specimen yield stiffness about 4 % lower than DE1 reference specimen yield stiffness. Also, DE5 specimen exhibited ductile behavior and was fractured due to bending fracture. Upon the increase of the number of anchorages used in a strengthening collapsing manner of test specimens changed and load capacity and ductility thereof increased.

Experimental study of masonry infill reinforced concrete frames with and without corner openings

  • Khoshnoud, Hamid Reza;Marsono, Kadir
    • Structural Engineering and Mechanics
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    • v.57 no.4
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    • pp.641-656
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    • 2016
  • Reinforced concrete frame buildings with masonry infill walls are one of the most popular structural systems in the world. In most cases, the effects of masonry infill walls are not considered in structural models. The results of earthquakes show that infill walls have a significant effect on the seismic response of buildings. In some cases, the buildings collapsed as a result of the formation of a soft story. This study developed a simple method, called corner opening, by replacing the corner of infill walls with a very flexible material to enhance the structural behavior of walls. To evaluate the proposed method a series of experiments were conducted on masonry infill wall and reinforced concrete frames with and without corner openings. Two 1:4 scale masonry infill walls with and without corner openings were tested under diagonal tension or shear strength and two RC frames with full infill walls and with corner opening infill walls were tested under monotonic horizontal loading up to a drift level of 2.5%. The experimental results revealed that the proposed method reduced the strength of infill wall specimens but considerably enhanced the ductility of infill wall specimens in the diagonal tension test. Moreover, the corner opening in infill walls prevented the slid shear failure of the infill wall in RC frames with infill walls.

Development of diagnostic method for human Astrovirus with rapid, specific and high sensitivity using loop-mediated isothermal amplification method

  • Lee, Jin-Young;Rho, Jae Young
    • Korean Journal of Agricultural Science
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    • v.47 no.1
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    • pp.173-182
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    • 2020
  • Human Astrovirus (HuAstV), known as a waterborne virus, is a group IV positive-sense single-stranded RNA that belongs to Astroviridae. The first outbreak of HuAstV was reported in England in 1975. HuAstV can exist not only among clinical patients but also in various water environments, such as water for agriculture and vegetables. For diagnosis of HuAstV from water samples, a polymerase chain reaction (PCR) system has been developed. However, the PCR-based diagnostic method has problems in field application, such as reaction time, sensitivity and specificity. For this reason, in this study we developed the loop-mediated isothermal amplification assay (LAMP) system, aimed specifically at HuAstV. Three prepared LAMP primer sets were tested by specificity, non-specificity and sensitivity; one LAMP primer set was selected with optimum reaction temperature. The developed LAMP primer set reaction conditions were confirmed at 62℃, and detection sensitivity was 1 fg/μL. In addition, restriction enzyme HaeIII (GG/CC) was introduced to confirm that the LAMP reaction was positive. As a result, selected LAMP primer set was 100 - 1000 times more specific, rapid, and sensitive than conventional-nested PCR methods. For verification of the developed LAMP assay, twenty samples of cDNA from groundwater samples were tested. We expect that the developed LAMP assay will be used to diagnose HuAstV from various samples.

The Primary Study on Chiljung of Inherent Basic Emotion (내재된 기본 감정으로서의 칠정(七情)에 관한 초보적 연구)

  • Jung, Jong-Hyo;Choi, Geum-Ae;Kim, Kyung-Su;Kim, Kyeong-Ok
    • Journal of Oriental Neuropsychiatry
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    • v.20 no.1
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    • pp.107-118
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    • 2009
  • Objectives : One's mind turned over by environment and personal relationship is Emotion. Emotion is composed by several basic feelings. This basic feeling is called Chiljung in Oriental Medicine. This study is confirmed difference of Chiljung by Constitution. Methods : 80 persons of Oriental medicine university students were tested by Qustionnaire for the Sasang Constitution Classification II(QSCCII) and Thematic Apperception Test(TAT). Constitution is distinguished by QSCCII. 11 pieces picture of TAT were showed one minute and then had person to discribe one's feelings. Two data were analyzed by statistical analysis. Results : Result is as follows, tested TAT about expression of inherent basic emotion. 1. It has relations with cognitive thought process 2. Expression of Chiljung has some relations with constitution and personal trait. Conclusion : Chiljung of inherent basic emotion is cognitive and has difference between constitutions by TAT.

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Basic Characteristics of an Active Controlled Capillary for Compensating the Error Motion of Hydrostatic Guideways (유정압안내면 운동오차보정용 능동제어모세관의 기본특성)

  • Song, Y.C.;Park, C.H.;Kim, S.T.
    • Journal of the Korean Society for Precision Engineering
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    • v.14 no.8
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    • pp.130-136
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    • 1997
  • For compensating the error motion of hydrostatic guideways, we introduce a way that the clearance of table is actively controlled corresponding to the amount of error with the variable capillary. The structure and the theoretical design method of active controlled capillary using piezo actuator, named ACC, are proposed in this paper. Basic characteristics such as the maximum controllable range, micro step response and available dynamic bandwidth are tested for confirmation of structural suitability of ACC, and these characteristics are also tested on the table mounted with ACC for verifying the availability. The experimental result showed that by the use of ACC, the error motion within 2.7 .mu. m of a hydrostatic guideway can be compensated with the resolution of 2.7nm, 1/100 contollable range, and the frequency bandwidth of 5.5 Hz. From these results, it is confirmed that the ACC is very effective to improve the motion accuracy of high or ultra precision hydrostatic guideways.

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Estimation of influening factors for efficient anaerobic digestion of high strength ammonia-nitrogen wastewater (고농도 암모니아성 질소 폐수의 효과적인 혐기성 처리를 위한 영향 인자 평가)

  • Park, Seyong;Park, Junghoon;Na, Hoysung;Kim, Moonil
    • Journal of Korean Society of Water and Wastewater
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    • v.26 no.5
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    • pp.649-658
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    • 2012
  • In this study, the influencing factors for efficient anaerobic digestion of high strength ammonia-nitrogen wastewater removal were investigated by testing biochemical methane potential test. In the influencing factors, the trace metals which could increase activity of anaerobic microorganisms, microbial concentration and types were evaluated. In the results, trace metals supplementation showed gas production amount higher than those without addition of trace metals. Among the tested trace metals, B, Ni, and Se were preferable to gas production. In the result of gas production according to the microbial concentration, the amount of gas production was proportional to the microbial concentration. In addition, the shortest lag time and the fastest gas production rate were achieved when the highest microbial concentration was tested. granule-type microorganism produced more gas than suspended-type microorganism. In conclusion, the efficient anaerobic digestion for high strength ammonia-nitrogen wastewater removal could be achieved by applying necessary trace metals injection and high concentration granule type microorganism.

Effect of a pathogenic bacteria filtration instrument for infection prevention during mouth-to-mouth ventilation (입-입 인공호흡(Mouth-to-mouth ventilation)을 위한 감염방지 도구의 병원성 세균 여과 효과)

  • Shim, Gyu-Sik;Kim, Eun-Mee
    • The Korean Journal of Emergency Medical Services
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    • v.20 no.3
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    • pp.49-56
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    • 2016
  • Purpose: The purpose of this study was to investigate the effect of a pathogenic bacteria filtration instrument for infection prevention during mouth-to-mouth ventilation. Methods: Two kinds of face shields were used for the study. One rescuer blew the filter through a bag valve mask and the filter was then cultured for bacteria. The mask was tested both on the front and back side. Results: Two kinds of face shields including the KF shield and CM shield were tested. The KF shield has received national certification and it prevented transmission of bacterial infection but the CM shield showed the opposite result and did not prevent bacterial transmission. Pathogenic bacteria were found on the back of the CM shield. Conclusion: A certified face shield is very important to prevent bacterial transmission. Face shields should be demonstrated and used by paramedic students.

Species Identification of Five Penaeid Shrimps Using PCR-RFLP and SSCP Analyses of 16S Ribosomal DNA

  • Khamnamtong, Bavornlak;Klinbunga, Sirawut;Menasveta, Piamsak
    • BMB Reports
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    • v.38 no.4
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    • pp.491-499
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    • 2005
  • DNA-based molecular markers for differentiation of five penaeid shrimps (Penaeus monodon, P. semisulcatus, Feneropenaeus merguiensis, Litopenaeus vannamei and Marsupenaeus japonicus) were developed based on polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and single-stranded conformation polymorphism (SSCP) of 16S ribosomal (r) DNA. Differentiation of P. monodon, P. semisulcatus and L. vannamei can be unambiguously carried out by PCR-RFLP of 16S $rDNA_{560}$ whereas P. semisulcatus and M. japonicus shared a BABB mitotype. These shrimps were successfully discriminated by SSCP analysis of 16S $rDNA_{560}$. Nevertheless, the amplification success for L. vannamei and F. merguiensis was not consistent when tested against larger sample sizes. As a result, 16S $rDNA_{560}$ of an individual representing the most common mitotype of each species was cloned and sequenced. The new primer pair was designed and tested against the large sample sizes (312 bp product, N = 185). The amplification success was consistent across all species. PCR-RFLP of 16S $rDNA_{312}$ was as effective as that of 16S $rDNA_{560}$. Differentiation of all shrimp species were successfully carried out by SSCP analysis.

Synthesis and Application of Caffeic Acid Methoxy Polyethylene Glycol Ester (카페산 메톡시 폴리에틸렌글리콜 에스테르의 합성 및 응용)

  • Lee, Hyun-Gi;Jeong, Noh-Hee
    • Journal of the Korean Applied Science and Technology
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    • v.28 no.3
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    • pp.259-266
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    • 2011
  • Caffeic acid is a kind of phytochemicals occurred in coffee, which is worked as a carcinogenesis restrainer and antioxidants on the human body. In this study, as one type of caffeic acid derivatives, methoxypolyethylene glycol caffeate(MPC) was synthesized by the esterification between caffeic acid and methoxypolyethylene glycol with N,N'-dicyclohexyl carbodiimide, 4-dimethylamino pyridine. The synthesized product was confirmed by using FT-IR and $^1H$-NMR, thin-layer chromatography. And these compound was investigated as antioxidant, Tyrosinase hindrance and skin moisturizers. In the free radical scavenging study, antioxidant effect of MPC was averagely high than the red ginseng extract that be used as a natural antioxidants. The result of Tyrosinase anti-activity test was better than embryo bud of rice extract at low concentration. At the iNOS anti-activity tested by using Raw 264.7 cell, and confirmed anti-inflammatory function. For the MPC handled with sodium lauryl sulfate, was tested for the skin moisture content and skin moisture loss.

A Study on the Development of Brassiere Pattern for Elderly Women (노년기 브래지어 패턴 개발)

  • Na, Mi-Hyang
    • Korean Journal of Human Ecology
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    • v.18 no.2
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    • pp.397-406
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    • 2009
  • A Study on the Development of Brassiere Pattern for Elderly Women The purpose of this study is to develop functional and sensible brassiere for elderly women. For this purpose, 6 elderly women(aged between 65 and 69) were sampled to be measured for their body sizes and tested for their bressiere wearing. he results were as follows; 1. In order to develop the basic patterns of elderly women's bressieres, 90B size was selected and then, their bressiere fitting was tested. The foundation pattern of the size 90A was proved for the body by the wearing tests. Each angle and length of the parts on the basic line of the breasts played an important role on setting the pattern. 2. As based on the body surface shell extracted from a plastic mold(photo.2), the cup of brassiere pattern were applied to the body surface shell(photo.4), and full side stretch-wings were applied to 13% reduced body size. The design pattern of elderly women's brassiers were characterized by a round wired as well as a full cup(3piece) embracing the entire breasts. 3. As the result of the wearing test, the excellence of the experimental brassiere was recognized objectively, with high marks in all the items(fig. 6). The experimental brassiere was covering the whole breasts to cope with change according to breasts loosing. It was designed to support the breasts firmly.