• Journal of Marine Bioscience and Biotechnology
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• v.11 no.2
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• pp.81-88
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• 2019

In this study, effects of nitrogen (N) and phosphorus (P) starvation on the cell growth and fatty acid (FA) production of newly isolated freshwater microalgae were investigated. The microalgae were identified as Chlorella sp. and Parachlorella sp. through 18S rRNA sequencing. Optimal culture temperature and light intensity were investigated using a high-throughput photobioreator, and the result was validated in 0.5 L bubble column photobioreactors using BG-11 without NaNO₃ and/or K₂HPO₄. Under nutrient starvation conditions, total FA contents of the microalgae were significantly changed rather than FA composition. Starvation of both N and P was most effective for increasing FA contents in Parachlorella sp (24.4±0.1%) whereas highest FA contents (42.6±1.8%) was achieved when only P was starved in Chlorella sp. among tested conditions. These results suggest an effective strategy for increasing FA production from microalgae using appropriate nutrient starvation.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.32 no.8
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• pp.29-36
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• 2004

Supersonic inlet buzz can be defined as unstable subcritical operation associated with fluctuating internal pressures and a shock pattern oscillating about the inlet entrance. The flow pulsations could result in flameout in the combustor or even structural damage to the engine. An experimental study was conducted to investigate the phenomenon of supersonic inlet buzz on axisymmetric, external-compression inlet. An inlet model with a cowl lip diameter of 30mm was tested at a free stream Mach number of 2.0. Subcritical instability was investigated by considering the frequency of pressure pulsation and shock wave structure at the inlet entrance. The results obtained show that total pressure recovery ratios were varied from 0.42 to 0.78, and capture area ratio from 0.34 to 0.98. The frequency of the subcritical flow increased with decrease in capture area ratios. Frequency was measured at $224{\sim}240Hz$.

• Journal of Bio-Environment Control
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• v.7 no.3
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• pp.237-245
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• 1998

Greenhouse crop production under critical summer climate In Korea has considerable difficulties because of high temperature and relative humidity. In this study, some water pipes were tested as a means of the dehumidification and increment of evaporative cooling efficiency. As a result of heat transfer characteristic analysis, overall heat transfer coefficient of copper pipe was larger than steel pipe, and estimated values were smaller than measured values. The condensed quantities of vapor were not significantly different between copper pipe and steel pipe, however dehumidifying effect by the water pipes was significantly large. It was estimated based on the results that the evaporative cooling system by the water pipe will be able to increase the evaporative cooling efficiency of about 48%, and decrease the temperature of about 1.3$^{\circ}C$.

• Journal of Korea Society of Industrial Information Systems
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• v.7 no.3
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• pp.43-53
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• 2002

The purpose of this study is to build a special -purpose browser using the powerful Microsoft web browser control so that we will be able to code and create a special-purpose browser. For this development, some literature surveys were reviewed to understand how the web browser looks like and is operated. To create our own web browser, we have designed a sample case and coded in Visual Basic. The primary task of this job was accomplished mainly with a form. It is made up with a bunch of image controls, a status bar on the button, a picture box to contain the image buttons, the combo box, the command button, the menus, the statements shown in the following exhibits and tables of the main text respectively. After combining them all together, we have tested the form to see if it works properly.'rho program was also enhanced to have a special purpose feature that permits the filtering function. The result was somewhat successful. However, it had some limitations such as the simplicity of the programming structures and functions. Overall, this study was useful for understand a general outline of special-purpose web browser development.

• Korean Journal of Food Science and Technology
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• v.33 no.4
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• pp.451-454
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• 2001

Fish paste containing mushroom, Agaricus bisporus, was prepared for the supplementary value of the product, and it was tested for the color, textural properties, and sensory attributes. With increasing amounts of the mushroom in fish paste, L value decreased and a value increased in the color changes while the b value was not significantly different. As the result of textural properties, folding test in all test samples showed AA that mean good flexibility. The hardness, elasticity, gumminess and brittleness of fish paste with the mushroom were increased but the strength was not significantly different. In overall acceptance of sensory evaluation, fish paste containing 10% of mushroom received the highest score. These results suggest that Agaricus bisporus can be applied to fish paste for the purpose of the varieties of product.

• Microbiology and Biotechnology Letters
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• v.26 no.4
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• pp.358-364
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• 1998

New microbial-control agents were prepared with B. thuringiensis strain NT0423 having unique properties which are different with other B. thuringiensis strains belonging to serotype 7[Kor. J. Appl. Entomol. 32: 426-432.]. Three B. thuringiensis formulations designated as BioBact 10%, 20% and 40%, were made with various combinations of adjuvants. These formulations showed good physical properties in wettability, suspensibility, particle size and adherence. In addition the result of SDS-PAGE analysis indicated that $\delta$-endotoxins remain stably in all formulations. Among the tested formulations, two wettable powder formulations, BioBact 20% and 40%, comprising 20% and 40% of B. thuringiensis technical powder showed the effective control against diamondback moth larvae (Plutella xylostella) in laboratory and field tests. Especially, when compared with commercial B. thuringiensis formulations (A and B commercial formulations) in field evaluation, BioBact 20% and 40% formulations showed equal activity up to 80% lethality and a good persistence effect which remain on leaves at least 7 days.

• The Journal of the Korea Contents Association
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• v.6 no.7
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• pp.1-12
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• 2006

This study examined relationship of effect between the characteristics of specialized portal and two branch dimension(cognitive and emotional e-trust) of e-trust on the specialized portal web-site. The characteristics of specialized portal web-site composed the guarantee factor, the structural factor, information factor, the entertainment factor and the flow factor by prior research. The model of e-trust strategy for specialized portal web-site is tested here using data from 122 samples. Result of factor and regression analysis show that the hypothesized relationships are significant. In examining the relationships of the factors in the study, the guarantee, structural and information fact effect on building of cognitive e-Trust. Second, the entertainment and flow factor effect on emotional e- Trust. Third, the cognitive and emotional e- Trust effect on the satisfaction of e-Service users. As these results, this study found out difference paths of e-Trust which effect on the satisfaction of e-Service by cognitive and emotional e- Trust approaches to the specialized portal web - sites.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2000.11c
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• pp.578-585
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• 2000

Geometrical characteristics of fresh red pepper(Capsicum annuum L.) were measured and indexed to define some important geometrical characteristics, and malformation of body and fruit stalk which are necessary for the design of the equipments for cutting, spreading and alignment of red pepper in developing a fruit stalk remover of fresh red pepper. The effects of bending of body and fruit stalk on the equipments of cutting, spreading and alignment were studied. The maximum lengths of some parts of fresh red pepper were found to be 180 mm, 125 mm, 144 mm, 67 mm and 76 mm for the body, the bent part of body, the fruit stalk, the bent part and the straight part of fruit stalk, respectively. The fresh red pepper with bending indices more than 0.4 and 0.3 for the body and the fruit stalk, respectively, was defined to be malformed based on the result of cutting rate using cutting unit; while the other ones to be normal in shape. Based on this, among the total fresh red peppers tested, 47%, 40% and 20% were found malformed for the body, the fruit stalk, and for both of the body and the fruit stalk. Malformed red peppers were poorer in spreading and alignment than normal ones, and the processed quantity was decreased with increased feed rate. The required time for the malformed peppers to pass on the alignment plate inclined at 30 increased rapidly at 8.3 Hz with increased feed rate. For the fresh red peppers with average moisture content of 85%,w.b., the maximum tensile strength between fruit stalk and body was 88.1 N; the maximum cutting resistances were 92.1 N and 94.9 N for the fruit stalk-calyx joint and body, respectively. Average coefficients of static friction were 0.99, 0.62, 0.59 and OJ, respectively, for the surfaces of rubber, galvanized iron, acryl and plywood.

• Journal of Biosystems Engineering
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• v.2 no.1
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• pp.56-80
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• 1977

This experimental work was conducted in order to find out the optimum time of harvest of Japonica-type (Akibare) and Indica-type(Tong-il) rice variety for three harvesting systems by investigating the harvesting losses and milling quality. The study was also concerned about the nature and amount of grain losses incurred during the each sequence of post-harvest technologies, and based on these result, a modification of existing systems giving a minimum grain loss was attempted. Binder, combine, and traditional systems were tested in this study and five grain moisture levels were disposed according to the decrease of grain moisture . The results are summarized as follows ; 1. The total losses of Akibare variety were ranged from 1.1 to 1.5 per cent for the traditional harvesting system , 2.1 to 4.8 per cent for the harvesting system by use of binder, and 2.8 to 4.3 per cent for the harvesting system by use of combine as the grain moisture content was reduced from 24 to 15 percent. Milling recovery of the harvesting system by use of binder, 74.8 ∼75.7 percent, was a little higher as it was compared to that of traditional harvesting system, 74.3 ∼75.0per cent, and that of the harvesting system by use of combine, 73.8 ∼75.0 per cent. Head rice recovery of mechanically dried paddy samples was higher than that of sun-dried paddy samples. 2. The total losses of Tong-il variety were ranged from 3.8 to 5.0 per cent for the traditional harvesting system, 5.2 to 10.0 percent for the system by use of binder, and 3.0 to 5.0 per cent for the system by use of combine as the grain moisture was reduced from 28 to 16 per cent. 3. Milling recovery of Tong-il variety harvested by the traditional harvesting system was 72.3 ∼73.6 percent and it was lower when compared to that of 72.3∼75.0 per cent harvested by binder, and 73.0∼74.6 percent harvested by combine. 4. Head rice recovery of Tong-il variety harvested by the traditional harvesting system (58.

• Biomedical Science Letters
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• v.11 no.4
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• pp.487-492
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• 2005

The baculovirus/insect cell expression system is of great value in the study of structure-function relationships in mammalian glucose-transport proteins by site-directed mutagenesis and for the large-scale production of these proteins for mechanistic and biochemical studies. Spodoptera frugiperda Clone 21 (Sf2l) cells grow well on TC-100 medium that contains $0.1\%$ D-glucose as the major carbon source, strongly suggesting the presence of endogenous glucose transporters. However, very little is known about the properties of the endogenous sugar transporter(s) in Sf2l cells, although a saturable transport system for hexose uptake has been previously revealed in the Sf cells. In order to further examine the substrate and inhibitor recognition properties of the Sf2l cell transporter, the ability of hexoses to inhibit 2-deoxy-D-glucose (2dGlc) transport was investigated by measuring inhibition constants $(K_i)$. The $K_i's$ for reversible inhibitors were determined from plots of uptake versus inhibitor concentration. Transport was effectively inhibited by D-mannose and D-glucose. Of the hexoses tested, L-glucose had the least effect on 2dGlc transport in the Sf2l cells, indicating that the transport is stereoselective. Unlike the human HepG2 type glucose transport system, D-mannose had a somewhat greater affinity for the Sf2l cell transporter than D-glucose, implying that the hydroxyl group at the C-2 position is not necessary for strong binding. However, epimerization at the C-4 position of D-glucose (D-galactose) resulted in a dramatic decrease in affinity of the hexose for the Sf2l cell transporter. Such a lowering of affinity might be the result of the involvement of the C-4 hydroxyl in hydrogen bonding. It is therefore suggested that Sf2l cells were found to contain an endogenous sugar transport activity that in several aspects resembles the human HepG2 type glucose transporter, although the insect and human transporters do differ in their affinity for cytochalasin B.