• Journal of the Korean Society for Library and Information Science
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• v.38 no.1
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• pp.35-50
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• 2004

This study evaluated the applicability of the static hierarchic clustering model to clustering query results in OPAC. Two clustering methods(Between Average Linkage(BAL) and Complete Linkage(CL)) and two similarity coefficients(Dice and Jaccard) were tested on the query results retrieved from 16 title-based keyword searchings. The precision of optimal dusters was improved more than 100% compared with title-word searching. There was no difference between similarity coefficients but clustering methods in optimal cluster effectiveness. CL method is better in precision ratio but BAL is better in recall ratio at the optimal top-level and bottom-level clusters. However the differences are not significant except higher recall ratio of BAL at the top-level duster. Small number of clusters and long chain of hierarchy for optimal cluster resulted from BAL could not be desirable and efficient.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.98-98
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• 2003

Recently, human embryonic stem (hES) cells have become very important resources for ES cell basic research, cell replacement therapy, and other medical applications; thus, efficient cryopreservation methods for these cells are needed. This study examined whether a newly developed minimum volume cooling (MVC) vitrification method, which was tested through cryopreservation of sensitive bovine oocytes, can be used for freezing hES cells. Feeder-free cultured hES cell (MB03) colonies were mechanically dissected into several small clumps following enzymatic treatment. We compared the freezing efficiency of a slow-cooling method using a cryo-module (0.4-0.6C/min, 20-30 clumps/vial) and MVC vitrification using a modified 0.5-ml French mini-straw designated as a MVC straw (>$20,000{\circ}C$/min, 10 clumps/straw) After thawing, in vitro survival of hES cell clumps was higher for MVC-vitrified cells (80.8%, 97/120) than for slow-cooled cells (38.2%, 39/102). Further, the proliferation rate of surviving MVC-vitrified cells was similar to that of control hES cells from 2 weeks after thawing. In addition, vitrified-thawed hES cells demonstrated a normal karyotype, were positively immunostained for surface marker antibodies (AP, SSEA-4 and TRA-1-60) and the Oct-4 antibody, and could differentiate into all three embryonic germ layer cells in vitro. This result demonstrates that hES cell clumps can be successfully cryopreserved by a newly developed MVC vitrification method without loss of human cell characteristics.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.71-71
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• 2003

Human umbilical cord blood cells(HUCBC) are rich in mesenchymal progenitor cells, endothelial cell precursors and hematopoietic cells. HUCBC have been used as a source of transplantable stem and progenitor cells. However, little is known about survival and development of HUCBC transplantation in the CNS. Estrogen has a neuroprotective potential against oxidative stress-induced cell death so has an effect on reducing infarct size of ischemic brain. We investigated the potential use of HUCBC as donor cells and tested whether estrogen mediates intravenously infused HUCBC enter and survive in ischemic brain. PKH26 labeled mononuclear fraction of HUCBC were injected into the tail vein of ischemic OVX rat brain with or without $17\beta$-estradiol valerate(EV). Under fluorescence microscopy, labeled cells were observed in the brain section. Significantly more cells were found in the ischemic brain than in the non-ischemic brain. HUCBC transplanted into ischemic brain could migrate and survive. Some of cells have shown neuronal like cells in hippocampus, striatum and cortex tissues. These result suggest that estrogen reduces ischemic damage and increases the migration of human umbilical cord blood cells. This Study was supported by the Korea Science and Engineering Foundation(KOSEF) though the Biohealth Products Research Center(BPRC), Inje University, Korea.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.120.3-121
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• 2003

Relative degree of resistance of citrus to Xanthomonas axonopodis pv. citri, the causal bacterium of canker, was investigated. Growth rate of a bacterium in leaf tissues after infiltration, disease incidence, and percent of lesion area were compared. By using growth rate[(GR=(At - A$\sub$t-1/)/A$\sub$t-1] host plants were differentiated into susceptible and resistant. Growth rates reached to peak at 40 hrs after inoculation and then declined. The growth rate in leaf tissues of a moderately susceptible cultivar, Citrus sinensis vu. Lane late(sweet orange), was the highest, and those of C. unshiu ${\times}$ C. sinensis(kiyomi), C. junos(yuzu), [(Citrus. unshiu x C. sinensis) x C. reticulata] (shiranuhi), and C. unshiu(satuma mandarin) were similar. This result indicates that the growth rate of the bacterium in leaf tissues can be effectively used for evaluation of disease resistance for citrus plants to X. axonopodis pv. citri. The disease on sweet orange occurred earlier than relatively resistant citrus plants tested. The percent of lesion area on leaf was also higher in sweet orange than those of satsuma mandarin, shiranuhi and kiyomi, and yuzu. The disease severity was highest on sweet orange and followed by kiyomi, shiranuhi, satsuma mandarin, and yuzu.

• Korean Journal Plant Pathology
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• v.13 no.3
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• pp.172-178
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• 1997

Xanthmonas campestris pv. campestris, causal agent of Black rot of crucifers, were isolated and identified from crucifer host. In order to determine the characters of X. c. pv. campestris associated with pathogenicity, Tn5 mutagenesis was carried out by conjugating with E. coli pJB4J1. Transconjugants were plate- assayed for missing cellulase, protease and amylase activity. A cellulase negative mutant was selected and tested for pathogenicity. Light microscopy and Scanning electron microscopy revealed that substomatal tissues were colonized by mutant, but was far less extensive than those by wild type. Stomatal surface and substomatal tissue appeared to have degraded by only wild type in 24 hrs and progression of pathogenesis was distinct in 48 hrs. In 6 days, wild type proliferated well in the tissue facilitated by cellulase activity. As a result, cellulase was determined as the important factor in pathogenesis.

• Journal of Life Science
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• v.18 no.2
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• pp.284-286
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• 2008

Octopus minor (O. minor) is widely distributed along the coastal regions of Korea, but most of them are caught in southern waters which are associated with one of the important fisheries stock. At present, O. minor from China has been introduced to the fishery markets in Korea. Here, we attempt to discriminate their origin for Korea or China using molecular techniques. Based on the O. minor mitochondrial DNA sequence, we developed a PCR-based origin discrimination system. The assay specificity was assessed by testing four individuals of O. minor from Sangdong, China, as well as 20 additional O. minor from Namhae, Muan, Yeosu and Jindo, Korea. Only four isolates of O. minor originated from China tested as positive in our distinction system. All PCR-positive products yielded identical sequences from Chinese O. minor, whereas Korean O. minor appeared to be PCR amplification. This result suggested that the primers used in this study are O. minor species specific, especially originated from China. The detection system appeared to be positive results in the use of 0.1 ng of Chinese O. minor DNA as template, however, the Korean O. minor even using $1{\mu}g$ of DNA showed no amplification. Consequently, the assay provides a simple, rapid and accurate method for the detection of Chinese O. minor.

• Food Science and Preservation
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• v.8 no.2
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• pp.206-212
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• 2001

Water extract of green tea(GTW) and 70% ethanol extract of green tea(GTE) were prepared for the test of antibacterial activity. The sensitivity of Staphylococcus aureus and Salmonella typhimurium to the green tea extracts in various pH of culture broth was tested. Tryptic soy broth(TSB) containing 0∼2%(w/v) of green tea extracts was adjusted to pH 5.0∼7.0 and inoculated with 10$\^$5/∼10$\^$6/ cells/ml of each bacteria. The plate counting method and clear zone test were used to determine inhibitory effect of green tea extracts. Green tea extracts completely inhibited the growth of S. aureus at 0.5% level and bactercidal at 0.5∼1.0% level of GTW and GTE at pH 5.0∼7.0. Green tea extracts were bactercidal to S. typhimurium at 1.5∼2.0% level of GTW and 1.0∼2.0% level of GTE at pH 7.0. Sal. typhimurium was more resistant than S. aureus. in same concentration of green tea extracts at same pH. The resistance of S. aureus and Sal. typhimurium was increased with decreasing pH of culture broth. The morphology of S. aureus cells treated with green tea extracts showed damage of cell wall and cytoplasmic membrane. Severely damaged cells of S. aureus lost electron dense material and cytoplasm. Green tea extracts stimulated autolysis and cell death of S. aureus. This result suggests that green tea extracts can be used as an effective natural antibacterial agent in food.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.43 no.2
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• pp.87-100
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• 2007

This study was carried out to offer fundamental data for improving the fishing efficiency of the Danish seine. The net height and the shape in the water was measured to analyze the efficiency of the existing Danish seine. And then, an improved fishing gear was developed based on the results and was tested in the field. Measuring devices were attached on center of a ground rope and a head rope. The net height is the spread distance between the ground rope and the head rope, which was measured on the different ratio of buoyancy. The results are obtained as follows. The net height estimated from the design plan of horizontal hanging ratio 0.40 in the existing Danish seine A and B estimated both 4.94m. The net height of the existing Danish seine A and B was respectively 1.8m and 2.3m, which form 36.4% and 46.2% of the net height estimated from the design plan. Buoyancy was changed as 79.5% and 96.2% relative to the sinking force in the existing Danish seine. The net height of 79.5% was 3.95m which increased to 80% of the estimated net height. The other shows the same result with the first case. It is not necessarily that the high buoyancy/sinking force ratio make the high net height, 80% is adequate as the buoyancy/sinking force ratio. In case of the improved Danish seine, the mean net height was about 5.0m, means 58.3% of estimated net height 8.58m.

• Korean Journal of Medicinal Crop Science
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• v.14 no.3
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• pp.163-167
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• 2006

The various cultural practices of bulbils were tested for seed tuber production in Chinese yam (Dioscorea batatas Decne.). To obtain amount of seed tuber, yam bulbils were sowed from early April to early May. The late sowing reduced average tuber weight, and consequently the amount of small tubers less than 20g increased highly. The optimum seeding rate of bulbils was 200 kg/10 a for production of seed-tubers. The average tuber weight was decreased according to increasing of seeding rate, so the amount of small tubers among total harvested tubers increased greatly at high seeding rate. Tuber size was affected by bulbil size. Seeding of the larger size bulbils result in increased sprouting rate, each tuber weight and tuber yield per unit area.

• Journal of The Korean Association of Information Education
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• v.5 no.1
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• pp.145-156
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• 2001

The purpose of this study is to set up the situated learning model for computer education and to investigate which method has better effect on the students' computer skill and learning attitude among the situated learning model and the traditional learning model. The result of this study is as follows. In order to investigate the effect of the students' learning attitude, students had been tested on six factors: the Understanding, the Interest, the Achievement, the Concentration, the Applicability, and the Spontaneity. As for the Understanding, the traditional learning model has better effect on students than the situated learning model. But the situated learning model was much superior in the other factors to the traditional learning model. Next, it had been examined how much students improved their computer skills under the situated learning model and under the traditional learning model. The study showed that the traditional learning model resulted in a little bit higher scores than the situated learning model. However, it was a great success to find out that the situated learning model is superior in the students' learning attitude to the traditional learning model.