Proceedings of the Korean Society of Developmental Biology Conference (한국발생생물학회:학술대회논문집)
- 2003.10a
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- Pages.98-98
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- 2003
A New Protocol for Effective Cryopreservation of Human Embryonic Stem Cells by a Minimum Volume Cooling Method
- Kim, Eun-Young (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Lee, Keum-Sil (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Shin, Hyun-Ah (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Park, Sae-Young (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Yoon, Ji-Yeon (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Kil, Kwang-Soo (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Lee, Young-Jae (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
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Kim, Nam-Hyung
(Chungbuk National University) ;
- Chung, Kil-Saeng (Konkuk University)
- Published : 2003.10.01
Abstract
Recently, human embryonic stem (hES) cells have become very important resources for ES cell basic research, cell replacement therapy, and other medical applications; thus, efficient cryopreservation methods for these cells are needed. This study examined whether a newly developed minimum volume cooling (MVC) vitrification method, which was tested through cryopreservation of sensitive bovine oocytes, can be used for freezing hES cells. Feeder-free cultured hES cell (MB03) colonies were mechanically dissected into several small clumps following enzymatic treatment. We compared the freezing efficiency of a slow-cooling method using a cryo-module (0.4-0.6C/min, 20-30 clumps/vial) and MVC vitrification using a modified 0.5-ml French mini-straw designated as a MVC straw (>