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Effect of Polyamines on Cellular Differentiation of N. gruberi: Inhibition of Translation of Tubulin mRNA

  • Yoo, Jin-Uk;Kwon, Kyung-Soon;Cho, Hyun-Il;Kim, Dae-Myung;Chung, In-Kwon;Kim, Young-Min;Lee, Tae-Ho;Lee, Joo-Hun
    • Journal of Microbiology
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    • v.35 no.4
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    • pp.315-322
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    • 1997
  • The effects of a polyamine, spermine, on the differentiation of Naegleria gruberi amebas into flagellates were tested. Addition of spermine at early stages of differentiation (until 40 min after the initiation of differentiation) completely inhibited the differentiation. To understand the inhibition mechanism, we examined the effect of spermine treatment on the transcription and translation of differentiation-specific genes during differentiation. Addition of spermine at early stages did not inhibit the accumulation of two differentiation-specific mRNAs, ${\alpha}$-tubulin and Class I mRNA, significantly, but rather prevented the rapid degradation of the mRNAs in later overall protein synthesis partially and gradually. However, translation of the ${\alpha}$-tubulin mRNA was completely inhibited. These data suggest that the inhibition of differentiation of N. gruberi by spermine treatment did not result from the inhibition of transcription of differentiation-specific genes but from the specific inhibition of translation of the mRNAs during the differentiation.

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Detection of Vibrio alginolyticus in Penaeus chinensis Using an Indirect fluorescent Antibody Technique

  • Heo, Moon-Soon;Lee, Sang-Hyeon;Son, Hong-Joo;Choon, Bok-Song;Lee, Je-Hee;Yeo, In-Okyy;Lee, Jung-Jae
    • Journal of Life Science
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    • v.10 no.1
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    • pp.24-27
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    • 2000
  • For the rapid diagnosis of vibriosis in penaeid shirmp, the indirect fluorescent antibody technique(IFAT) was established to detect Vibrio alginolyticus. The titers of the antisera used for this experiment were above 1280. Vibrio alginolyticus possesses the specific antigen, and also have antigens shared with other strains. When an V. alginolyticus-infected adult shirmp was tested by IFAT, V. alginolyticus was detected mainly in the muscle tissues near the injection point and the haemolymph but only few in other tissues. This result indicates that the pathogen bacteria could be detected by IFAT. Thus, it is suggested IFAT is more convenient and sensitive method than conventional plate method for the diagnosis of induced Vibrio infection in the penaeid shrimps.

Thermal Shock Resistance Property of TaC Added Ti(C,N)-Ni Cermets (TaC 첨가 Ti(C,N)-Ni 서멧의 내열충격 특성)

  • Shin, Soon-Gi
    • Korean Journal of Materials Research
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    • v.24 no.10
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    • pp.526-531
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    • 2014
  • Thermal shock resistance property has recently been considered to be one of the most important basic properties, in the same way that the transverse-rupture property is important for sintered hard materials such as ceramics, cemented carbides, and cermets. Attempts were made to evaluate the thermal shock resistance property of 10 vol% TaC added Ti(C,N)-Ni cermets using the infrared radiation heating method. The method uses a thin circular disk that is heated by infrared rays in the central area with a constant heat flux. The technique makes it possible to evaluate the thermal shock strength (Tss) and thermal shock fracture toughness (Tsf) directly from the electric powder charge and the time of fracture, despite the fact that Tss and Tsf consist of the thermal properties of the material tested. Tsf can be measured for a specimen with an edge notch, while Tss cannot be measured for specimens without such a notch. It was thought, however, that Tsf might depend on the radius of curvature of the edge notch. Using the Tsf data, Tss was calculated using a consideration of the stress concentration. The thermal shock resistance property of 10 vol% TaC added Ti(C,N)-Ni cermet increased with increases in the content of nitrogen and Ni. As a result, it was considered that Tss could be applied to an evaluation of the thermal shock resistance of cermets.

The Study on Difference in Length Cognition Ability in Dominant Eye (우성안에 따른 길이식별 인지능력 차이에 관한 연구)

  • Nam, Kun-Woo;Park, Dae-Sung
    • Journal of Korean Physical Therapy Science
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    • v.15 no.4
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    • pp.11-17
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    • 2008
  • Background: Human body is formed of symmetric bilateral structures that are comprised of eye, upper arm, lower arm and etc. but, we are used only dominant components. The purpose of this study was to analysis length cognition ability in dominant eye. Methods: Total 88 persons (male 18, female 70) were participated in this study. They were tested with ‘hole in the card’ test for identification of dominant eye's side, then the length cognition ability was measured in right & left axillary level by describing 10cm line. Results: The results by independent t-test were as follows. In difference of length cognition ability in right axillary level between right dominant eyed group & left dominant eyed group, right dominant eyed group was superior to left dominant eyed group, but significant difference was not existed statistically(p>.05). In left axillary level, right dominant eyed group was superior to left dominant eyed group, but significant difference was not existed statistically(p>.05). Conclusion: These result can be applied to the learning of palpation & observation skill in physical therapy, although this study was not identify a relation between dominant eye & dominant hand.

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NUMERICAL SIMULATION OF FLOW AND HEAT TRANSFER IN A COOLING CHANNEL WITH STAGGERED V-SHAPED RIBS (엇갈린 V-형 리브가 부착된 냉각유로에서의 열유동 수치해석)

  • Myong, H.K.;Kim, K.Y.
    • Journal of computational fluids engineering
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    • v.13 no.4
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    • pp.107-113
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    • 2008
  • The present study numerically simulates the flow and heat transfer characteristics of rib-induced secondary flow in a square cooling channel with staggered V-shaped ribs, extruded on both walls. The rib pitch-to-height ratio (p/h) varies from 2.8 to 10 with the rib-height-to-hydraulic diameter ration (h/$D_h$)of 0.07 and the Reynolds number of 50,000. Shear stress transport (SST) turbulence model is used as a turbulence model. Computational results show that complex secondary flow patterns are generated in the channel due to the snaking flow in the streamwise direction for all tested cases. In the range of p/h=5 to 10 the staggered V-shaped rib gives about 3 times higher heat transfer augmentation than the reference smooth pipe with high heat transfer on both front side and the area around the leading edge of the ribs, while the former cases give about 18 times higher streamwise pressure drop than the latter ones. However, for the thermal performances, based on the equal pumping power condition, the case of p/h=2.8 gives the best result among three cases, mainly due to relatively low streamwise pressure drop, although it gives relatively low heat transfer augmentation.

In Vitro Antifungal Activities of Amphotericin B, Fluconazole, Itraconazole, Terbinafine, Caspofungin, Voriconazole, and Posaconazole against 30 Clinical Isolates of Cryptococcus neoformans var. neoformancs

  • Lee, Young-Ki;Fothergill, Annette W.
    • Mycobiology
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    • v.31 no.2
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    • pp.95-98
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    • 2003
  • Aantifungal agents were tested against 30 clinical isolates of Cryptococcus neoformans var. neoformans using the NCCLS method(M27-A2). Posaconazole, itraconazole and amphotericin B had lower MIC than the remaining four antifungal agents. The MIC result for posaconazole was over 220-fold lower active than fluconazole. Fluconazole MICs for most isolates fell within the dose-dependant range. The overall MIC ranges and $MIC_{50}s$ were amphotericin B(0.03-0.25; 0.25), fluconazole(0.5-64; 16), itraconazole(0.015-1; 0.125), terbinafine(0.06->2; 1), caspofungin(8-32; 32), voriconazole(0.015-0.5; 0.25), and posaconazole(0.015-0.25; 0.06 ${\mu}g/ml$), respectively. In conclusion, the $MIC_{50}s$ of these drugs did not exhibit any sign of an upward shift with the exception of fluconazole and tendency cross-resistance between the seven drugs was not observed. We conclude that in vitro resistance to antifungal agents has not significantly changed despite the recent wide-spread use of triazoles for long-term treatment of Cryptococcal meningitis.

Recycling Technology of Cementitious Powder for Completely Recycling of Concrete Waste (폐콘크리트의 순환이용을 위한 폐미분말의 재활용 기술)

  • Park, Cha-Won;Kang, Byeung-Hee
    • Journal of the Korea Institute of Building Construction
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    • v.5 no.3 s.17
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    • pp.109-116
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    • 2005
  • Recently, there have been many studies seeking towards the utilization of cementitious powder from concrete waste as recycled cement. However, most of the studies actually have been researches about the reuse of mortar or paste, not concrete waste. In fact, either mortar or paste is quite different from a real concrete waste in terms of age and mixture. Thus the purpose of this study is to examine basic physical properties of recycled cement, manufactured with cementitious powder from concrete waste, and analyze differences in chemical and hydraulic properties of the cement and its tested model. As a result of the chemical analysis, recycle cement is composed mainly of CaO and $SiO_2$, and that it is even lower in the content of CaO than Portland cement, which is also supported by previous studies. But, Differently from previous studies, calcining temperature of 650 was found an optimal condition under which cementitious powder from concrete waste could restore its hydraulic properties.

UV Sensitivity of Korean Skin and The Effects of Factors affecting SPF Determination (한국인 피부의 자외선 감수성과 SPF 측정치에 미치는 인자의 영향)

  • 이병곤
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.17 no.1
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    • pp.1-17
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    • 1991
  • Multiport-600 Solar SimulatorR is one of the most recent and convenient in-strument for evaluation of sun protection factor(SPF). In this study, we examined the practicability of the SPF determining system using Multiport -600 and the effects of several factors-light sources, seasons and experimental animals-on the minimal erythema dose(MED) and SPF. We also tested the UV sensitivity according to the sites of Korean people, And the ultraviolet radiation reaching the earth's surface In Seoul have been observed for one year. As a result of this study, the determinig system for SPF using Multiport-600 was proved to be a good system in accuracy and time-saving. The biological activity of fluorescence UV lamp of PUVA-800R was significantly higher than natural light or solar simulator with Xe arc lamp, and the determined MED became lower in inverse proportion to room temperature rise. Skin sensitivity by ultraviolet adiation was hights. in order \circled1 back \circled2 inns, upper arm \circled3 outer upper arm \circled4 foream. We also observed that UV radiation intensity was highest at noon in july and 1 sun burn unit(MED) was 28 minutes at that time.

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The Performance Measurement and Comparison for Real-Time Control Application of ARM920T (ARM920T의 Real-Time 제어적용을 위한 성능 측정 및 비교)

  • Kim, Taek-Ki;Park, Sang-Hyuk;Lim, Jae-Sik;Lee, Young-Il
    • Proceedings of the KIEE Conference
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    • 2008.04a
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    • pp.59-60
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    • 2008
  • In this paper, we investigate the ability of the ARM processor to implement an industrial controller with or without embedded operating system. Discrete-time PID controllers are implemented and tested under various settings e.g. cache on/off, different clock frequencies using S3C2410X chip. A method of real-time application of discrete-time PID controller in WinCE environment is proposed. Based on the test result, we provide the maximum sampling frequencies of PID controller using ARM processor.

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Mushrooms Collected from Deogyu Mountain, Muju, Korea and Their Antioxidant Activity

  • Kim, Seong-Eun;Lee, In-Kyoung;Jung, Yun-A;Yeom, Ji-Hee;Ki, Dae-Won;Lee, Myeong-Seok;Song, Ja-Gyeong;Jin, Yong-Ju;Seok, Soon-Ja;Yun, Bong-Sik
    • Mycobiology
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    • v.40 no.2
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    • pp.134-137
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    • 2012
  • Mushrooms collected from Deogyu mountain, Korea, in 2011, were identified as four classes, four orders, 13 families, 22 genera, and 33 species. In particular, agaricales was most abundant and comprised more than 70%. Their antioxidant activities were estimated using three different bioassay methods, the 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical scavenging assay, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assay, and reducing power assay. As a result, the methanol extracts of Stereum ostrea, Laetiporus sulphureus var. miniatus, and Tyromyces sambuceus exhibited potent antioxidant activity in all bioassays tested.