Journal of Life Science
- Volume 10 Issue 1
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- Pages.24-27
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- 2000
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- 1229-1552(pISSN)
Detection of Vibrio alginolyticus in Penaeus chinensis Using an Indirect fluorescent Antibody Technique
- Heo, Moon-Soon (Corresponding author, Faculty of Applied Marine Science, Cheju National University) ;
- Lee, Sang-Hyeon (Division of New Materials and Biotechnology, Silla University) ;
- Son, Hong-Joo (Department of Biotechnology, Miryang National University) ;
- Choon, Bok-Song (Faculty of Applied Marine Science, Cheju National University) ;
- Lee, Je-Hee (Faculty of Applied Marine SCience, Cheju National University) ;
- Yeo, In-Okyy (Faculty of Applied Marine Science, Cheju National University) ;
- Lee, Jung-Jae (Faculty of Applied Marine Science, Cheju National University)
- Published : 2000.04.01
Abstract
For the rapid diagnosis of vibriosis in penaeid shirmp, the indirect fluorescent antibody technique(IFAT) was established to detect Vibrio alginolyticus. The titers of the antisera used for this experiment were above 1280. Vibrio alginolyticus possesses the specific antigen, and also have antigens shared with other strains. When an V. alginolyticus-infected adult shirmp was tested by IFAT, V. alginolyticus was detected mainly in the muscle tissues near the injection point and the haemolymph but only few in other tissues. This result indicates that the pathogen bacteria could be detected by IFAT. Thus, it is suggested IFAT is more convenient and sensitive method than conventional plate method for the diagnosis of induced Vibrio infection in the penaeid shrimps.