• Korean Journal of Fisheries and Aquatic Sciences
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• v.45 no.6
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• pp.654-658
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• 2012

Vibrio alginolyticus, a marine fish and shellfish pathogen, has been found at a high frequency around the coastal areas of Korea. Vibrio alginolyticus was purified from various diseased fish, and a V. alginolyticus-specific bacteriophage was isolated from seawater obtained from fish farms located on the west coast of Korea. In a bacterial lysis experiment using the phage and antibiotics, tetracycline, $10^3$ cfu/ml of V. alginolyticus were completely lysed by both the phage and the antibiotic, suggesting that the purified phage in the study could be utilized as an alternative to antibiotics in the control of fish and shellfish diseases caused by V. alginolyticus.

• Journal of Life Science
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• v.10 no.1
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• pp.24-27
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• 2000

For the rapid diagnosis of vibriosis in penaeid shirmp, the indirect fluorescent antibody technique(IFAT) was established to detect Vibrio alginolyticus. The titers of the antisera used for this experiment were above 1280. Vibrio alginolyticus possesses the specific antigen, and also have antigens shared with other strains. When an V. alginolyticus-infected adult shirmp was tested by IFAT, V. alginolyticus was detected mainly in the muscle tissues near the injection point and the haemolymph but only few in other tissues. This result indicates that the pathogen bacteria could be detected by IFAT. Thus, it is suggested IFAT is more convenient and sensitive method than conventional plate method for the diagnosis of induced Vibrio infection in the penaeid shrimps.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.38 no.6
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• pp.365-371
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• 2005

The antimicrobial resistance patterns to the 10 antimicrobial agents of potential pathogenic vibrios isolated from seawater and a variety of farmed fishes, including oliver flounder (Payalichthys olivaceus), black rock fish (Sebastes schlegeli), red sea bream (Pagyus major) and sea bass (Lateolabyax japonicus), were investigated from May to October, 2004. A total of 314 strains of the genus vibrios were isolated from 126 collected samples, and the number of isolated strains of Vibrio payahaemolyticus and Vibrio alginolyticus were 194 and 120, respectively. Apparently $98.5\%$ of V. parahaemolyticus and $100\%$ of V. alginolyticus isolates demonstrated antimicrobial resistance against at least one antimicrobial agent. The resistance of V. parahaernolyticus isolates to ampicillin ($97.9\%$) was highest, followed by oxolinic acid ($26.8\%$), amikacin ($19.1\%$) and tetracycline and amoxicillin/clavulanic acid ($6.7\%$). V. alginolyticus isolates were resistant to ampicillin ($100\%$), solfamethoxazoleit,imethopenem ($25\%$), amikacln ($21.7\%$), amoxicillin/clavulanic acid ($15.8\%$), ciprofloxacin ($13.3\%$), and tetracycline and doxycycline ($11.7\%$). The rate of multiple antimicrobial resistance to at least four antimicrobials was higher in the V. alginolyticus isolates ($20.8\%$) than in the V. parahaemolyticus ($6.7\%$).

• Journal of fish pathology
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• v.25 no.3
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• pp.165-172
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• 2012

Vibrio alginolyticus (V. alginolyticus), which is one of bacterial pathogens evoking severe infection in fish and shellfish as well as in human has been found at high frequency around all coast areas in Korea. Both V. alginolyticus and V. alginolyticus specific bacteriophage were isolated from sea water and various fishes from fish farms on west coast in Korea. In a morphological study based on electron microscope, the purified phage appeared to be composed of hexagon head of 60 nm and short tail of 20 nm. In the denatured SDS-PAGE analysis, the structural proteins of the phage were found to be 7 different protein fractions ranging from 37.8 to 198 kda. The kind of nucleic acid of the phage was ascertained to a double stranded DNA.

• The Journal of the Korean Society for Microbiology
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• v.22 no.4
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• pp.413-425
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• 1987

In the present experiments, isolated Vibrio species from marine and clinical specimens from July, 1985 to October, 1986, had the results as follows: 1. The 55 strains of Vibrio were isolated and identified; Vibrio parahaemolyticus was 35 strains, Vibrio vulnificus was 10 strains, Vibrio alginolyticus was 10 strains. 2. In the K-serotyping of Vibrio parahaemolyticus, fourteen serotypes identified but three were not strains typable by the availble K-antisera. 3. In the Kanagawa phenomenon experiment of Vibrio parahaemolyticus, it proved positive reaction, 14 of 15 strains(93%) isolated from the patient and 13 of 20 strains(65%) isolated from the nature. 4. In twelve antibiotic resistance experiments, Vibrio parahaemolyticus and Vibrio alginolyticus showed 100% resistance on ampicilline, but Vibrio vulnificus showed 100% sensitivity. But all of them proved 100% sensitivity on chloramphenicol, tetracycline, nalidixic acid. 5. In the antibiotic resistance patterns, Vibrio parahaemolyticus proved that 15 strains(43%) resisted on 4 antibiotics and 5 strains(14%) resisted on 7 antibiotisc and. Vibrio vulnificus proved that 1 strain(10%) resisted on 2 antibiotics and 6 strains(60%) without resistance, Vibrio alginolyticus proved that 7 strains(70%) resisted on 3 antibiotics and 2 strains(20%) resisted on 8 antibiotics.

• Microbiology and Biotechnology Letters
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• v.27 no.3
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• pp.203-207
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• 1999

V. alginolyticus 138-2, a marine halophilic bacterium, produced an extracellular amylase with a molecular weight of ca. 56,000. The analysis of the digestion products of soluble starch by thin layer chromatography(TLC) revealed that the extracellular amylase of V. alginolyticus 138-2 is a saccharifying-type alpha-amylase. The alpha-amylase activity of the culture supernatant of soluble starch was optimal at pH 6.0 and 45$^{\circ}C$. Ca2+ slightly increased the alpha-amylase activity, whereas Hg2+, An2+, Cu2+, Ni2+, Fe2+, and Mn2+inhibited the enzymatic activity. Alkylating thiol group agent, iodoacetic acid did not affect the alpha-amylase activity, but reduced thiol reagents such as dithiothreitol, cysteine, and beta-mercaptoethanol stimulated theenzymatic activity. On the other hand, even if V. alginolyticus 138-2 is a marine halophilic bacterium, its alpha-amylase activity was significantly inhibited by NaCl.

• Journal of Life Science
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• v.22 no.2
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• pp.245-250
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• 2012

The 16S rDNA - RFLP types for six Vibrio species (V. fluvialis, V. proteolyticus, V. vulnificus, V. mimicus) including two core group members, V. alginolyticus and V. parahaemolyticu s, and Grimontia (Vibrio) hollisae were determined using PCR-RFLP analysis. Six tetrameric restriction enzymes (Alu I, Cfo I, Dde I, Hae III, Msp I, and Rsa I) were selected for RFLP analysis. V. alginolyticus, V. parahaemolyticus, and V. proteolyticus showed the same RFLP pattern following digestion with four of the six used restriction enzymes: CfoI, DdeI, MspI, and RsaI. Various restriction enzyme combinations generated digests recognizable as distinct RFLP types for each of the assayed Vibrio species. In particular, AluI single digestion produced species specific band patterns that enabled the differentiation between these Vibrio species. Dendrogram based on restriction patterns showed that two Vibrio core group members, V. alginolyticus and V. parahaemolyticus were closely related having a similarity over 90%. Although the observed RFLP pattern for Grimontia hollisae shared several common bands with other Vibrio spp., G. hollisae results were still clearly distinct from Vibrio spp. RFLP types for all restriction enzymes tested. If restriction enzymes are aptly selected, PCR-RFLP analysis is still a rapid and effective tool for differentiating Vibrio species.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.9 no.4
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• pp.233-237
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• 1976

This study was carried out to evaluate the distribution of Vibrio parahaemolyticus and Vibrio alginolyticus in sea water, mud, oyster (Crassostrea gigas) and sea mussel (Mytilus edulis) collected from the coast of Chung-mu during the period from July 1975 to September 1976. Fifty one strains of V. parahaemolyticus and 160 strains of V.. alginolyticus were isolated from 420 samples. The distribution varied by month showing the highest in July through September The isolation ratio of V. parahaemolyticus was $28\%$ for mud, $24\%$ for sea water, $5\%$ for sea mussel and $4.2\%$ for oyster. The morphological, physiological and biochemical characteristics of 211 isolated strains were coincided with those of the typical V. parahaemolyticus and V. alginolyticus.

• Journal of Marine Bioscience and Biotechnology
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• v.13 no.1
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• pp.48-57
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• 2021

Vibrio species are Gram-negative basophils that are ubiquitous in seawater, increasing in number as the water temperature increases. Humans are usually infected by the consumption of contaminated seawater or seafood. V. alginolyticus infection in humans is mainly associated with infections of the skin and ears, such as acute otitis media and cellulitis. In this study, the distribution of V. alginolyticus along the coast of Jeju Island, and its relationship with water temperature, salinity, DO, and pH was investigated. The antibiotic susceptibility of the bacteria isolated was also tested. In seawater, the Daejeong area had the highest detection rate, with 13 cases (21.7%), and the Hallim area showed the lowest detection rate, with eight cases (13.3%) in. In shellfish, the Daejeong area had the highest rate, with seven cases (23.3%), and the Seongsan and Hallim areas had the lowest detection rate, with four cases (13.3%). The overall detection rate was the highest in Daejeong area, with 20 cases (22.2%), and the lowest in the Hallim area, with 12 cases (13.3%). The detection rate was highest when the water temperature was highest.

• Journal of Microbiology and Biotechnology
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• v.20 no.2
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• pp.271-280
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• 2010

Vibrio alginolyticus, a Gram-negative marine bacterium, is one of the causative agents of fish vibriosis. Its virulence factors and pathogenesis mechanism are barely known, except for some extracellular products (ECPs) that are known to be regulated by quorum sensing system. Therefore, the present study used a microarray to analyze the transcription profiles of the wild-type V. alginolyticus and a deletion mutant of luxO, the pivotal regulator in Vibrio quorum sensing systems, which resulted in the identification of a putative virulence factor, MviN. Quantitative real-time reverse transcription PCR confirmed that the transcription of mviN was upregulated in the luxO mutant when compared with wild-type, and down regulated in a luxO-con complemented strain. Furthermore, Western blotting indicated that MviN was greatly induced during the late-exponential and stationary phases of growth, indicating that the expression of MviN was cell-density dependent and quorum sensing regulated in V. alginolyticus. Meanwhile, the mviN null mutant displayed a much slower growth rate than the wild type, signifying the essential role of MviN in V. alginolyticus. Western blotting also revealed that MviN was present as an extracellular protein in V. alginolyticus. When epithelioma papulosum cyprini (EPC) cells were treated with the ECPs of the mviN mutant, no cytotoxicity was observed, whereas EPC cells treated with the wild type exhibited pathological changes, which increased with the ECPs concentration and treatment time. Therefore, the results demonstrated that MviN is a LuxO-regulated ECPs component and involved in the pathogenicity of V. alginolyticus.