• International Journal of Industrial Entomology and Biomaterials
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• v.29 no.2
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• pp.207-213
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• 2014

The structural proteins of classical swine fever virus (CSFV) consist of nucleocapsid protein C and envelope glycoprotein $E^{rns}$ (E0), E1 and E2. Among them, E2, the most immunogenic of the CSFV glycoproteins, induces a protective immune response in swine. In this study, to determine the optimal expression conditions of glycoprotein E2 using baculovirus system, we investigated the influence of insect cells and media to the expression of recombinant E2. Recombinant virus containing glycoprotein E2 coding gene was constructed with bApGOZA DNA. Expression of the glycoprotein E2 was analyzed by SDS-PAGE and Western blot analysis using anti-CSFV E2 monoclonal antibodies. Expression of glycoprotein E2 in Sf21 cells was first observed after 3 days and reached a maximum on the 5th day after infection. Furthermore, the highest levels of glycoprotein E2 expression were observed at multiplicity of infection (MOI) of 5. When three different insect cell lines (Sf21, High-Five and Se301) were tested, High-Five cells showed the highest production. In addition, four different serum-free and serum-supplemented media, respectively, were tested for the expression of glycoprotein E2 and the budded virus (BV) titers. As a result, serum-supplemented medium provided the best conditions for protein production and the BV yield.

• Journal of the Korean Society of Propulsion Engineers
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• v.14 no.2
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• pp.10-18
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• 2010

Scramjet engine is one of the core parts of hypersonic vehicle of next generation and being investigated by many countries. Korea Aerospace Research Institute performed a ground test of the model scramjet engine S1 in 2007. And, S2 model which is improved from S1 model in engine startability and thrust was tested with HIEST (High Enthalpy Shock Tunnel) at Kakuda Space Center of JAXA. Design condition of S2 model was Mach 6.7, however, it was tested at Mach 7.7 as an off-design condition test. As a test result, flow separation was found at the inside of the intake, but the engine showed stable combustion pressure distribution. Furthermore, compared to other test models, S2 model showed a good performance value in thrust and specific impulse.

• Earthquakes and Structures
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• v.8 no.3
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• pp.761-778
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• 2015

The objective of this paper is to report the result of an experimental program conducted on the strengthening of nonductile RC frames by using external mesh reinforcement and plaster application. The main objective was to test an alternative strengthening technique for reinforced concrete buildings, which could be applied with minimum disturbance to the occupants. Generic specimen is two floors and one bay RC frame in 1/2 scales. The basic aim of tested strengthening techniques is to upgrade strength, ductility and stiffness of the member and/or the structural system. Six specimens, two of which were reference specimens and the remaining four of which had deficient steel detailing and poor concrete quality were strengthened and tested in an experimental program under cyclic loading. The parameters of the experimental study are mesh reinforcement ratio and plaster thickness of the infilled wall. The effects of the mesh reinforced plaster application for strengthening on behavior, strength, stiffness, failure mode and ductility of the specimens were investigated. Premature and unexpected failure mode has been observed at first and second specimens failed due to inadequate plaster thickness. Also third strengthened specimen failed due to inadequate lap splice of the external mesh reinforcement. The last modified specimen behaved satisfactorily with higher ultimate load carrying capacity. Externally reinforced infill wall composites improve seismic behavior by increasing lateral strength, lateral stiffness, and energy dissipation capacity of reinforced concrete buildings, and limit both structural and nonstructural damages caused by earthquakes.

• Mycobiology
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• v.48 no.4
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• pp.326-329
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• 2020

Valuable natural compounds produced by a variety of microorganisms can be used as lead molecules for development of new agrochemicals. Furthermore, high-throughput in vitro screening systems with specific modes of action can increase the probability of discovery of new fungicides. In the current study, a rapid assay tested with various microbes was developed to determine the degree of respiratory inhibition of Saccharomyces cerevisiae in two different liquid media, YG (containing a fermentable carbon source) and NFYG (containing a non-fermentable carbon source). Based on this system, we screened 100 fungal isolates that were classified into basidiomycetes, to find microbial secondary metabolites that act as respiratory inhibitors. Consequently, of the 100 fungal species tested, the culture broth of an IUM04881 isolate inhibited growth of S. cerevisiae in NFYG medium, but not in YG medium. The result is comparable to that from treatment with kresoxim-methyl used as a control, suggesting that the culture broth of IUM04881 isolate might contain active compounds showing the inhibition activity for respiratory chain. Based on the assay developed in this study and spectroscopic analysis, we isolated and identified an antifungal compound (-)-oudemansin A from culture broth of IUM04881 that is identified as Oudemansiella venosolamellata. This is the first report that (-)-oudemansin A is identified from O. venosolamellata in Korea. Taken together, the development of this assay will accelerate efforts to find and identify natural respiratory inhibitors from various microbes.

• Journal of Fashion Business
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• v.24 no.3
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• pp.17-29
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• 2020

This study aimed to produce fiber stretch sensors for smart soccer socks to prevent injuries during training. A sensor was manufactured with stretchable fabric and tested to ensure convenience during training. In order to manufacture the fiber stretch sensor, a CNT dispersion solution was applied to an e-band and elastic polyester fabric, and the performance of the sensors was evaluated by a tensile test. Performance evaluation showed that both of the tested fabrics are excellent for this purpose. Both sensors were attached to socks to create prototype wearable devices, and an experiment was conducted to determine whether a resistance change accompanying relaxation and contraction of the gastrocnemius muscle could be detected. In order to accurately evaluate performance as a sensor, the fabric was stretched 20 times at low speeds of 1 Hz and 0.5 Hz. A change in resistance due to tension was observed, with both the E-band and the stretchable poly fabric showing high sensitivity and high reproducibility. Both can be used as relaxation/contraction sensors. Smart soccer socks were made using the two materials, and an evaluation was conducted. Tensile tests were done on the smart soccer socks; the tests were done 20 times per sock, and the sensor showed a stable resistance change between 30 and 40 ohms depending on the tension of the sensor. As a result, we confirmed that smart soccer socks with stretch sensors made of E-bands can measure changes in the gastrocnemius muscle.

• Journal of Microbiology
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• v.33 no.4
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• pp.339-343
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• 1995

We isolated a 10 kb Bam HI fragment originated from the chromosome of a $H_2O$$^2$-resistant mutant strain of Streptomyces coelicolor, which confer $H_2O$$^2$-resistance to S. lividance upon transformation. Among various subclones ot 10kb Bam HI fragment tested for their $H_2O$$^2$-resistant phenotype in S. lividans, a subclone containing 5.2 kb Bam HI-BglII fragment was found to be responsible for $H_2O$$^2$-resistance. The plasmid containing this 5.2 kb fragment was then transformed into S. coellicolor A3(2) at early and tested for their phenotype of $H_2O$$^2$-resistance and the change in various enzymes whose activity can be stained in the gel. We found out that the 5.2 kb insert DNA conferred $H_2O$$^2$-resisstance in S. coelicolor A3(2) at early phase of cell growth. The presence of this DNA also resulted in higher level of peroxidase compared with the wild type cell containing parental vector (pIJ702) only. Esterase activity was also higher in this clone. However, alcohol dehydrogenase activity decreased compared with the wild type. These results suggest that the presence of a gene in 5.2 kb BamHI-BglII DNA fragment causes multiple changes in S. coelicolor related to its response against hydrogen peroxide. The result also implies that not only peroxidase but also esterase may function in the defencse meahsnism agianst $H_2O$$^2$-.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.12
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• pp.5457-5463
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• 2011

The objective of this study is to investigate cooling performance characteristics of mobile air conditioning system using R744 as an alternative of R-134a. In order to analyze the cooling performance characteristics of the air conditioning system using R744 for a passenger vehicle, the developed air conditioning system using R744 was applied in a real passenger vehicle and tested under various operating conditions with the variation of gas-cooler inlet air conditions, evaporator inlet air temperatures and compressor speeds. As a result, cooling capacity and coefficient of performance (COP) of the tested air conditioning system decreased with the rise of the inlet air temperature of the gas cooler but increased with the rise of the inlet air temperature of the evaporator. In addition, cooling capacity and coefficient of performance (COP) increased by 42.2 % with the rise of the compressor speed from 900 rev/min to 1800 rev/min, but it decreased by 55.4%.

• Journal of Soil and Groundwater Environment
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• v.19 no.3
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• pp.104-110
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• 2014

In order to study gross alpha analysis method using LSC, the efficiency tests with uranium standard materials were performed and then compared with the GPC method (US EPA 900.0 method) using 15 groundwater samples. For 15 groundwater samples, the average efficiencies of the GPC and LSC method were 7~11% and 90%, respectively. The average precisions of the GPC and LSC method were 16.16% and 6.00%, respectively. Also, The average standard deviations for 15 samples were 7.38 pCi/L and 2.95 pCi/L, respectively. The determination coefficient of the tested results by two methods was 0.9948. As a result, the LSC method tested in this study was applicable for the screening of the gross alpha and showed the advantages in the gross alpha measurement due to the simple measurement procedures.

• Hwankyungkyoyuk
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• v.21 no.2
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• pp.80-88
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• 2008

As the gravity of the issue of the global warming surfaced, environmental protection and energy conservation have become one of the most serious issues that global society faces. At the status quo, there are criticisms for not effectively educating the students although the problem is very severe. In middle school, for instance, only 10% of the school offer environmental education while only $20{\sim}30%$ of the high school offer it. As witnessed by these examples, we can infer that there are not well-structured, effective environmental education for teenagers. Recently, NIE(Newspaper-In-Education) has been used as effective means for some fields of middle and/ or high school education. If so, it also could be used for environmental education. The researches, however, on the topic of NIE on environmental education are very rare. In this study, the effect of NIE on environmental education was empirically tested. Our basic assumption is that NIE will cause differences in environmental perception; the cognition and concern. And environment education through NIE will cause the high level of cognition, concern, and practice regarding environment issue. Using simple experimental design and regression analysis, we tested the effect of NIE on cognition, and, concern of environment issue, and then, on the practice of environment-improving action. The "cognition level" did not show significant difference between the experimental group(which was exposed to NIE) and control group(which was not exposed to NIE). The "concern level" for environment showed significant difference. Finally, the "practice level" showed highly significant difference between the experimental and the control group. According to regression analysis, "concern" and "NIE"(dummy variable) was turned out to be statistically significant variables for practice of environment improving action. Therefore, we came up with the conclusion that NIE, which provides the most up-to-date information regarding the environmental status, can serve as the good supplementary mechanism for the effective environment education in the present Korean middle school curriculum system. The statistical result also suggests that each individual school needs to employ and extend NIE method in its environment education curriculum.

• International Journal of Industrial Entomology and Biomaterials
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• v.21 no.1
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• pp.133-137
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• 2010

The perilla leaf pyralid moth, Pyrausta panopealis Walker (Lepidoptera: Pyralidae), is a serious pest damaging to leaf perilla. In order to establish the life parameters of P. panopealis for eventual purpose of control, the developmental span of each stage was investigated under five temperature regimes ($20^{\circ}C{\sim}30^{\circ}C$). The larval period of P. panopealis was longest as 26.8 days at $20^{\circ}C$ and shortened as temperature goes up to $30^{\circ}C$ as 11.3 days. Survivorship of the larval P. panopealis was the highest at $27.5^{\circ}C$ as 82.5%, whereas that of other temperatures ranged from 40% ($20^{\circ}C$) to 60.0% ($30^{\circ}C$), indicating that the P. panopealis appears to favor somewhat higher temperature. In addition to larval period, the duration of egg, prepupal, and pupal period also were shortened sharply as temperature goes up, whereas the duration of adult stage shortened a maximum of only two days as temperature goes up. After the perilla leaf pyralid moths were successfully stabilized in indoor environment the larvicidal efficacy of ten on-the-market environmentfriendly agricultural materials (EFAMs) that were previously selected from the result of other moth species was tested. Seven of the ten tested showed more than 90% of mortality within 12 hrs and reached nearly up to 100% within 24 hrs, but the remaining three showed less than ~70%.