Mycobiology

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

The Antifungal Test: An Efficient Screening Tool for the Discovery of Microbial Metabolites with Respiratory Inhibitory Activity

  • Han, Jae Woo (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Kim, Bomin (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Oh, Mira (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Choi, Jaehyuk (Division of Life Sciences, Incheon National University) ;
  • Choi, Gyung Ja (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology) ;
  • Kim, Hun (Center for Eco-friendly New Materials, Korea Research Institute of Chemical Technology)
  • Received : 2020.02.24
  • Accepted : 2020.04.30
  • Published : 2020.08.31
Download PDF
⟨ Previous Next ⟩

Abstract

Valuable natural compounds produced by a variety of microorganisms can be used as lead molecules for development of new agrochemicals. Furthermore, high-throughput in vitro screening systems with specific modes of action can increase the probability of discovery of new fungicides. In the current study, a rapid assay tested with various microbes was developed to determine the degree of respiratory inhibition of Saccharomyces cerevisiae in two different liquid media, YG (containing a fermentable carbon source) and NFYG (containing a non-fermentable carbon source). Based on this system, we screened 100 fungal isolates that were classified into basidiomycetes, to find microbial secondary metabolites that act as respiratory inhibitors. Consequently, of the 100 fungal species tested, the culture broth of an IUM04881 isolate inhibited growth of S. cerevisiae in NFYG medium, but not in YG medium. The result is comparable to that from treatment with kresoxim-methyl used as a control, suggesting that the culture broth of IUM04881 isolate might contain active compounds showing the inhibition activity for respiratory chain. Based on the assay developed in this study and spectroscopic analysis, we isolated and identified an antifungal compound (-)-oudemansin A from culture broth of IUM04881 that is identified as Oudemansiella venosolamellata. This is the first report that (-)-oudemansin A is identified from O. venosolamellata in Korea. Taken together, the development of this assay will accelerate efforts to find and identify natural respiratory inhibitors from various microbes.

Keywords

References

  1. Bartlett DW, Clough JM, Godwin JR, et al. The strobilurin fungicides. Pest Manag Sci. 2002;58(7):649-662. https://doi.org/10.1002/ps.520
  2. Becker WF, Von Jagow G, Anke T, et al. Oudemansin, strobilurins A, strobilurins B, and myxothiazole: new inhibitors of the bc1 segment of the respiratory chain with an E-β-methoxyacrylate system as common structural element. FEBS Lett. 1981;132(2):329-333. https://doi.org/10.1016/0014-5793(81)81190-8
  3. Ypema HL, Gold RE. Kresoxim - methyl: modification of a naturally occurring compound to produce a new fungicide. Plant Dis. 1999;83(1):4-19. https://doi.org/10.1094/PDIS.1999.83.1.4
  4. Corran AJ, Renwick A, Dunbar SJ. Approaches to in-vitro lead generation for fungicide invention. Pestic Sci. 1998;54(4):338-344. https://doi.org/10.1002/(SICI)1096-9063(199812)54:4<338::AID-PS824>3.0.CO;2-K
  5. Chiba M, Bown AW, Danic D. Inhibition of yeast respiration and fermentation by benomyl, carbendazim, isocyanates, and other fungicidal chemicals. Can J Microbiol. 1987;33(2):157-161. https://doi.org/10.1139/m87-027
  6. Sangkaew A, Krungkrai J, Yompakdee C. Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors. AMB Express. 2018;8(1):124. https://doi.org/10.1186/s13568-018-0653-9
  7. Zhou Y, Bian Q, Yang P, et al. Catalytic asymmetric syntheses of (-)-oudemanisin A and its diastereomer. Tetrahedron. 2017;28(7):969-973. https://doi.org/10.1016/j.tetasy.2017.06.002
  8. Tamura K, Nei M. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol. 1993;10(3):512-526.
  9. Saitou N, Nei M. The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987;4(4):406-425.
  10. Rosa LH, Cota BB, Machado KMG, et al. Antifungal and other biological activities from Oudemansiella canarii (Basidiomycota). World J Microbiol Biotechnol. 2005;21(6-7):983-987. https://doi.org/10.1007/s11274-004-7553-7
  11. Anke T, Hecht HT, Schramm G, et al. Antibiotics from basidiomycetes. IX. Oudemansin, an antifungal antibiotic from Oudemansiella mucida (Schrader ex Fr.) Hoehnel (Agaricales). J Antibiot. 1979;32(11):1112-1117. https://doi.org/10.7164/antibiotics.32.1112