• Title/Summary/Keyword: test species

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A survey on the prevalence of internal parasites in the Korean indigenous goats of Kyungbuk area (경북지역의 흑염소 내부기생충 감염률 조사)

  • 박노찬;도재철;김수웅;송해범
    • Korean Journal of Veterinary Service
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    • v.20 no.4
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    • pp.349-358
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    • 1997
  • From December 1995 to July 1997, a total of 546 Korean indigenous goats were examined for the presence of internal parasites by parenchymal organs and fecal examinations. Four hundreds seventy eight(87.5%) of the goats were infected with 4 classes and 12 parasitic species. The nematodes were Haemonchus contortus(57.5%), Oesophagostomun venulosum (52.6%), Strongyloides papillosus(48.4%), Ostertagia sp(28.4%), Trichostrongylus colubriformis(17.0%), and Trichuris ovis(13.2%). The trematodes were Paramphistomum sp(17.6%), Fasciola hepatica(15.4%), and Eurytrema Pancreaticum (8.8%). The cestode was Moniezia expansa(16.7%), and the protozoa were Eimeria sp(74.9%) and Toxoplasma gondii(8.6%). The incidence of single or mixed infections among 12 parasitic species were one species infection (6.2%), 2 species(11.5%), 3 species(19.6%), 4 species(24.9%), 5 species(17.8%), 6 species(5.9 %), and 7 species(1.6), respectively. The level of infection according to the age was considerably higher under one year(97.2%) than between one and two years(89.7%), and over two years(69.3%) Among 12 parasites, seasonal pattern was remarkably observed. Most paracites were detected at the highest level in the Winter, whereas Strongyloides papillosus and Paramphistomum sp were in the Spring, and Ostertagia sp and Fasciola hepatica were in the Autumn. Infection rates of Anaplasma sp were 8.6% of 210 goats by blood smear method, and 51.0% by complement fixation test.

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Selecting plant species for landfill revegetation: a test of 10 native species on reclaimed soils

  • Song, Uhram
    • Journal of Ecology and Environment
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    • v.42 no.4
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    • pp.259-264
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    • 2018
  • Background: Revegetating landfills can be a challenging task as the reclaimed soils are typically dry and have low nutrient content. Therefore, selecting suitable plant species is important for initial revegetation. The issue with current practices (in Korea) is that alien plant species have been typically selected for revegetation projects. In this context, this study selects and tests a set of native plant species for landfill revegetation, accompanied by the assessment of the landfill soils. Results: The soil of the landfill (landfill location) was in a very poor condition with high bulk density and low organic matter and nutrient contents. Among 10 tested species, only Brassica campestris showed high coverage and a sufficient number of individuals in study quadrats sown with seeds. Results suggest that plant species with heavy seeds are the only ones that can adapt to the environment of a typical landfill due to the site's aridity and low nutrient content. The reason is due to such species' superior wind resistance and the capacity to provide sufficient energy for the initial growth of the plants for survival in such landfill environment. Conclusions: This study recommends selecting plant species (1) with arid-adapted features and (2) whose seed weight is sufficiently heavy for survival at landfills or areas with a similar condition for future revegetation.

Screening Test on the Constituents from the Stems of the Genus Thalictrum in Korea (국산 Thalictrum 속 식물 지상부의 성분검색)

  • Lee, In-Ran
    • Korean Journal of Pharmacognosy
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    • v.3 no.1
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    • pp.23-25
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    • 1972
  • Preliminary investigations were carried out to isolate the constituents from the stems of genus Thalictrum. Methanol extracts of eight species from the genus were examined by thin layer chromatography. The author observed three different spots with Dragendorff's reagent in Thalictrum actaefolium, whereas none was detected in Thalictrum tuberiferum and Thalictrum simplex. Various fluorescent colors with different Rf values were detected in all species by UV ray, suggesting that each species may have different components.

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The Antibiotic Activities of Some Korean Lichenes (한국산지의류의 항균작용에 관한 연구)

  • 한세호
    • YAKHAK HOEJI
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    • v.10 no.4
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    • pp.7-20
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    • 1966
  • 1. Of the 32 extracts from Genus of lishenes broth tested for antimicrobial activity, 28 inhibited at least one of the 3 test microorganisms used. 2. Twenty seven lichnes broth from 32 species tested were active against at least one of the Gram-positive bacteria M. pyogenes var, aureus 203 p, and twenty four lichenes broth from 32 Species tested were active against at least one of the Gram-positive bacteria Bacillus subtilis ATCC 6633. 3 Twenty five lichenes broth from 32 species tested were active against at least one of the Gram-negative bacteria Escherichia coli 0.126. 4. The antibiotic substances in lichenes were readily extracted by organic solvents.

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Results of Secondary Antibiotics Susceptibility Test for Gram Negative Bacilli Resistant to Primary Susceptibility Test in Yeungnam University Hospital (기본 항생제에 내성을 보인 그람 음성 간균에 대한 2차적 항생제 감수성 검사 결과)

  • Kim, Chung-Sook;Kim, Kyung-Dong;Lee, Chae-Hoon
    • Journal of Yeungnam Medical Science
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    • v.10 no.1
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    • pp.28-36
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    • 1993
  • Major pathogenic Gram-negative organisms such as P. aeruginosa, Serratia species, E. coli, Enterobacter species which are isolated from the specimens in large medical centers are greatly resistant to the commonly used antibiotics. Gram-negative bacilli, which had been isolated in Yeungnam University Hospital during the period from December 1992 to April 1993 and turned out to be resistant to the primary antibiotics susceptibility test for chloramphenicol, ampicillin, cephalothin, gentamicin, tetracyclin, amikin and tobramycin, were subjected to the secondary antibiotics susceptibility test for aztreonam, ceftazidime, ciprofioxacine, cefotaxime, cefamandole, piperacillin, ticarcillin and sulfamethoxazole trimethopime. Out of 315 tested organisms, 167 organisms(53%) were resistant to all secondary antibiotics in vitro. Antimicrobial activity of ceftazidime(37.1%), aztreonam(11.%), ciprofioxacine(7.9%) against Gram negative bacilli were slightly more active than other antibiotics tested, while cefamandole was not active to all the Gram-negative bacilli tested. According to the specimens, E. coli was the most frequently resistant organisms to the primary antibiotics from urine, A. baumanii, from respiratory system and wounds, and P. aeruginosa from various specimens. In summary, Gram negative bacilli resistant to the primarily applied antibiotics also were resistant to the secondary antibiotics. Rearrangement of the antibiotics disks for the antibiotic susceptibility test should be considered.

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Phototoxicity: Its Mechanism and Animal Alternative Test Methods

  • Kim, Kyuri;Park, Hyeonji;Lim, Kyung-Min
    • Toxicological Research
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    • v.31 no.2
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    • pp.97-104
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    • 2015
  • The skin exposure to solar irradiation and photoreactive xenobiotics may produce abnormal skin reaction, phototoxicity. Phototoxicity is an acute light-induced response, which occurs when photoreacive chemicals are activated by solar lights and transformed into products cytotoxic against the skin cells. Multifarious symptoms of phototoxicity are identified, skin irritation, erythema, pruritis, and edema that are similar to those of the exaggerated sunburn. Diverse organic chemicals, especially drugs, are known to induce phototoxicity, which is probably from the common possession of UV-absorbing benzene or heterocyclic rings in their molecular structures. Both UVB (290~320 nm) and UVA (320~400 nm) are responsible for the manifestation of phototoxicity. Absorption of photons and absorbed energy (hv) by photoactive chemicals results in molecular changes or generates reactive oxygen species and depending on the way how endogenous molecules are affected by phototoxicants, mechanisms of phototoxcity is categorized into two modes of action: Direct when unstable species from excited state directly react with the endogenous molecules, and indirect when endogeneous molecules react with secondary photoproducts. In order to identify phototoxic potential of a chemical, various test methods have been introduced. Focus is given to animal alternative test methods, i.e., in vitro, and in chemico assays as well as in vivo. 3T3 neutral red uptake assay, erythrocyte photohemolysis test, and phototoxicity test using human 3-dimensional (3D) epidermis model are examples of in vitro assays. In chemico methods evaluate the generation of reactive oxygen species or DNA strand break activity employing plasmid for chemicals, or drugs with phototoxic potential.

Allelopathic Effects of Artemisia lavandulaefolia

  • Kil, B.S.;Han, D.M.;Lee, C.H.;Kim, Y.S.;Yun, K.Y.;Yoo, H.G.
    • The Korean Journal of Ecology
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    • v.23 no.2
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    • pp.149-155
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    • 2000
  • The allelopathic effects of Artemisia lavandulaefolia were studied using several test plants and microbes. Aqueous extracts and volatile compounds of A. lavandulaefolia inhibited seed germination, seedling and root growth of the test species such as Achyranthes japonica. Lactuca sativa, Artemisia princeps var. orientalis. Oenothera odorata, Plantago asiatica. Aster yomena, Elsholtzia ciliata, and Raphanus sativus var. hortensis for. acanthiformis. The root growth of test species was more affected than shoot growth by allelochemicals of A. lavandulaefolia. Essential oil of A. lavandulaefolia had antibacterial and antifungal effects. However, the antimicrobial activity of the essential oil was dependent upon the microbial species and concentrations. Callus growth of Oryza sativa, Brassica campestris subsp. napus var. pekinensis and Achyranthes japonica was sensitive by the essential oil of A. lavandulaefolia. Twenty three chemicals were identified from A. lavandulaefolia essential oil by gas chromatography. Primary allelochemicals among them were 1, 8-cineole, 1-$\alpha$-terpineol, $\alpha$-terpinene. camphor, 2-buten-1-ol and azulene. We concluded that aqueous extract and essential oil of A. lavandulaefolia were responsible for allelopathic effects.

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A Comparative Study of Species of Tree Applied to Scientific Name Questions of Engineer Landscape Architecture Examination and Landscape Planting Construction (조경기사 시험의 학명 문제와 조경식재공사에 적용되고 있는 수종의 비교 연구)

  • Jung, Yong-Jo
    • Journal of the Korean Institute of Landscape Architecture
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    • v.47 no.2
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    • pp.1-12
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    • 2019
  • The purpose of this study is to compare and analyze how many species of trees presented with scientific names are on the written test for engineer landscape architecture, as their usage has increased in the landscape industry in recent years. The following is the result of comparing and analyzing the species of trees which are frequently used in landscape, planting construction, and the scientific name questions presented in the previous 6 years by year on the written test for engineer landscape architecture. First, the results of analyzing the examination question frequency by year in the landscape planting subject showed a gradually declining trend from 2014 after peaking at 56.6% in 2013. Second, 163 species of trees using scientific names were presented in questions on the written test for engineer landscape architecture over the previous 6 years and these included Betula platyphylla, Sophora japonica, Cercis chinensis, Taxus cuspidata. The number of species of trees used in landscape construction during the same period was 169 including Pinus densiflora, Chionanthus retusus, Cornus officinalis, Syringa dilatata. The result of interviews with landscape planting construction officials showed that when choosing the species of trees, they emphasize ecological characteristics, such as the ratio of trees to shrubs, flowers and autumn leaf characteristics, and also consider the morphological characteristics, landscape characteristics, economic feasibility, and the supply and demand situation. Third, it was found that 70 (42.9%) out of 163 species of trees presented with scientific names on the examination were not used in landscape planting construction and 76 (44.9%) out of 169 species were not used as material for questions. As for the scientific name questions, deciduous trees and evergreen shrubs accounted for the more questions, Evergreen and deciduous tree and shrubs were most common in landscape planting construction as well.

Coating Effect of Low Temperature Melting Bi-Sn Metalized Wood Composites on 10 Different Korean Wood Species (주요 국산재 10가지 수종에 대한 Bi-Sn 저온용융 합금주입 목재복합체의 도장 효과)

  • Park, Kye-Shin;Seo, In Soo;Lee, Hwa Hyoung;Kang, Seog Goo
    • Journal of the Korea Furniture Society
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    • v.25 no.3
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    • pp.223-232
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    • 2014
  • To make metalized wood composites of 10 different korean wood species, it was tested by Low temperature melting Bi-Sn alloy injection method at high temperature and high pressure condition. Metalized wood composite of each wood species had light ash color, but still maintained its own natural wood grain and wood figures. It was evaluated on coating properties. Furthermore, it also was check on effect of coating as like a changes of main color, wood grain and wood figures. The results of test were following as; a coating properties as like a cold resistance, heat resistance, acid resistance, alkali resistance, durability of abrasion, and cross-cut test was proper on the conditions of the KS standard. And, In aspect of color changes, the metalized wood composites of 10 different korean wood species had a light ash color, but still maintained its own natural wood grain and wood figures. Also, the more weight per gain of alloy is, the more grey background of metalized wood composite is.

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Identification of Mycobacterium species by rpoB Gene PCR-RFLP (rpoB 유전자의 PCR-RFLP를 이용한 Mycobacterium 균종 동정의 유용성)

  • Yu, Kyong-Nae;Park, Chung-Ho
    • Korean Journal of Clinical Laboratory Science
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    • v.38 no.3
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    • pp.158-165
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    • 2006
  • Although Mycobacterium tuberculosis complex strains remain responsible for the majority of diseases caused by mycobacterial infections worldwide, the increase in HIV infections has allowed for the emergence of other non-tuberculous mycobacteria as clinically significant pathogens. However, Mycobacterium species has a long period of incubation, and requires serious biochemical tests such as niacin, catalase, and nitrate test that are often tedious. The development of rapid and accurate diagnostics can aid in the early diagnosis of disease caused by Mycobacterium. The current DNA amplification and hybridization methods that have been developed target several genes for the detection of mycobacterial species such as hps65, 16S rDNA, rpoB, and dnaj. These methods produce rapid and accurate results. In this study, PCR-restriction fragment length polymorphism analysis(PCR-RFLP) based on the region of the rpoB gene was used to verify the identification of non-tuburculosis Mycobacterium species. A total of 8 mycobacterial reference strains and 13 clinical isolates were digested with restriction enzymes such as Msp I in this study. The results of using this process clearly demonstrated that all 13 specimens were identified by rpoB gene PRA method. The PCR-RFLP method based on the rpoB gene is a simple, rapid, and accurate test for the identification of Mycobacterium.

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