• Title/Summary/Keyword: terminal segment

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A mutational anlaysis of the N-terminal protease of bovine viral diarrhea virus

  • Chon, Seung-ki
    • Korean Journal of Veterinary Research
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    • v.39 no.4
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    • pp.772-777
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    • 1999
  • The uncaped genomic RNA of bovine viral diarrhea virus (BVDV) initiates translation by recruitment of eukaryotic translation initiation factors at the internal ribosome entry site (IRES). N-terminal protease ($N^{pro}$) is the first translation product of the open reading frame (ORF). By using the vaccinia virus SP6 RNA polymerase transient expression system, we showed previously that deletion of $N^{pro}$ region reduced translation by 21%. To better understand the biological significance of $N^{pro}$ for translation, we carried out a mutational analysis of the $N^{pro}$ region of BVDV cloned in the intercistronic region of a bicistronic reporter plasmid. We constructed a bicistronic expression vector in which the entire 5 UTR and the mutated $N^{pro}$ region (${\Delta}386-901$, ${\Delta}415-901$ and ${\Delta}657-901$) was cloned between two reporter genes, chloramphenicol acetyltransferase (CAT) and luciferase (LUC). In vivo translation analyses showed that $N^{pro}$ region was dispensible for efficient translation. The results indicate that the $N^{pro}$ region is not essential for BVDV RNA translation and the 3' boundary of BVDV IRES is expanded into $N^{pro}$ region, suggesting that $N^{pro}$ may not play a major role in BVDV replication.

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DEVELOPMENT OF THE HAUSAT-2 PAYLOAD OF ANIMAL TRACKING SYSTEM (HAUSAT-2 소형 위성 동물 추적 시스템 탑재체 개발)

  • Lee Jeong-Nam;Lee Byung-Hoon;Moon Byung-Young;Chang Young-Keun
    • Bulletin of the Korean Space Science Society
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    • 2006.04a
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    • pp.129-132
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    • 2006
  • Animal Tracking System consists of Animal Tracking System Receiver on the Satellite segment, Animal Tracking Terminal and Ground Station for data analysis on the Ground segment. This paper describes operation concept and hardware design for Animal Tracking System which is the payload of HAUSAT-2 being developed by the Space System Research Laboratory (SSRL). Algorithms for determination of animal position and data processing are also referred to.

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Genogroup position of aquabirnavirus GC-1 isolated from rockfish Sebastes schiegeli in Korea

  • Joh, Seong-Joon;Lee, Youn-Jeong;Song, Chang-Sun;Kang, Shien-Young;Mo, In-Pil;Heo, Gang-Jun
    • Korean Journal of Veterinary Research
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    • v.48 no.3
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    • pp.287-293
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    • 2008
  • The cDNA of the aquabirnavirus, GC-1 isolated from rockfish Sebastes schlegeli in Korea, was synthesized using the reverse transcriptase-polymerase chain reaction. The nucleotide and deduced amino acid sequences were determined from cDNA of the VP2-NS-VP3 coding region of genome segment A. The nucleotide sequences of the segment A were 3,086 base pairs (bp) in length and contained large open reading frame (ORF) and terminal sequences. The large ORF was comprised of 2,916 bp nucleotides and composed of 972 deduced amino acid sequences. Pairwise comparisons were made with other aquabirnavirus sequences published previously. The study of genetic relationships between GC-1 and aquabirnaviruses in the large ORF and VP2 coding regions demonstrated that the GC-1 has the nearest genetic relationship with the marine birnaviruses (MABV strains), and the GC-1 and MABV strains can be clustered as the same genogroup. GC-1 can be included in MABV, which is the 7th genogroup of family Aquabirnaviridae.

A New Species of the Genus Bryocamptus (Copepoda, Harpacticoida, Canthocamptidae) from Alpine Wetlands at Jeju Island, Korea

  • Lee, Jimin;Chang, Cheon Young
    • Animal Systematics, Evolution and Diversity
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    • v.32 no.3
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    • pp.219-229
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    • 2016
  • Material of a Bryocamptus species, formerly reported as B. umiatensis Wilson, 1958 from Sakhalin and South Korea, is here recognized as a distinct new species. Specimens of both sexes were recently obtained from an alpine wetland, Sumeunmulbaengdui, located in the Hallasan National Park, Korea, and newly registered as a Ramsar Wetland in 2015. The new species, B. jejuensis n. sp., differs from the type population of B. umiatensis from Alaska by the smooth margin of the anal operculum in both sexes, the peculiarly modified terminal seta on the distal endopodal segment of the male leg 3, the sword-like spinous seta on the distal endopodal segment of the male leg 4, and the presence of a setule row on the inner distal margin of the caudal rami in the female. Both sexes of the new species are described in detail with particular reference to the male characters. A revised key to the seven species of the genus Bryocamptus Chappuis, 1929 occurring in Korean waters is provided.

External Ultrastructure of the Adult Genital Segments of Pine Needle Gall Midge, Thecodiplosis japonensis Uchida et Inouye (솔잎혹파리(Thecodiplosis japonensis Uchida et Inouye)에 관한 연구 II - 성충 생식절의 표면미색구조 -)

  • 한성식;박용철;이찬용
    • Korean journal of applied entomology
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    • v.27 no.3
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    • pp.144-148
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    • 1988
  • Using scanning electron microscope, we studied the adult genital segments of pine needle gall midge, Thecodiplosis Japonensis, Uchida et Inouye Male: There were two pairs of hypoproct a pair of claspettes and aedeagus, Hypoproct were surrounded with many setae and sensilla. Clasperres which was projected below hypoproct was composed of two aegments and had many sensilla Aedeagus was long and flattened and possesed some sensilla at the terminal. Female :Ovipositor was segmented into three parts. Each segment had a different externla sturcture; particularly, the 2nd one was covered with reversed boat-like attachments and did not show any sensillum.

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A Study on the Construction of Ground Test Segment for the Time Synchronization System Using the Geostationary Satellite (정지궤도 위성을 이용한 시각동기 지상시스템 시험장비 구축에 관한 연구)

  • Lee, Sang-Cherl;Kim, Bang-Yeop
    • Journal of the Korean Society for Aeronautical & Space Sciences
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    • v.32 no.5
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    • pp.104-108
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    • 2004
  • The most of the CDMA mobile communication depends on the GPS for the time synchronization. Then, we must prepare alternative system against the unusable GPS like a unexpectable accident or strategic purpose by the USA government. In this study, we have constructed ground test segment for the time synchronization system using the geostationary satellite. In addition. we have designed, manufactured and tested the transmitting and receiving board to receive 1 PPS signal from atomic clock for transmitting stored data in buffer to satellite modem and to produce 1 PPS signal from satellite modem for measuring time delay.

Copepods of the family Kelleriidae (Crustacea, Copepoda, Cyclopoida) from tropical waters of the Asia-Pacific

  • Hong, Jae-Sang;Kim, Il-Hoi
    • Journal of Species Research
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    • v.10 no.4
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    • pp.364-386
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    • 2021
  • Six species of the family Kelleriidae, including four new species, are recorded from tropical waters of the Asia-Pacific, two of them from Phuket Island, Thailand, one each from Bohol Island, Philippines and Phu Quoc Island, Vietnam, and two from Kosrae Island, Micronesia. A new genus Kelleriella is proposed to accomodate one of these new species (Kelleriella quadridens n. sp.). There are three diagnostic features of the new genus: the terminal segment of antenna is armed with one strong claw and six setae, the armature of the maxilliped endopod is reduced, and the third exopodal segment of leg 4 is armed with four spines and five setae. The other three new species are Kelleria latipes n. sp., Kelleria phuketensis n. sp., and Kelleria robusta n. sp. As diagnostic features of these new species, their caudal rami are about 2.5, 3.2, and 1.72 times longer than wide, respectively, the exopod of leg 5 of them is smooth, without any process, about 1.9, 3.7, and 2.9 times longer than wide, respectively, and armed with one spine plus one seta in Kelleria latipes and Kelleria phuketensis or with 2 setae in Kelleria robusta. Additionally, Kelleria javaensis Mulyadi, 2009 is synonymized with Kelleria regalis Gurney, 1927 and Kelleria grandisetiger Kim, 2006 is synonymized with Kelleria andamanensis Sewell, 1949, and both species are redescribed.

Copepods (Cyclopoida) Associated with Compound Ascidians (Tunicata) from Korea, with Descriptions of Nine New Species

  • Lee, Jimin;Kim, Il-Hoi
    • Animal Systematics, Evolution and Diversity
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    • v.38 no.4
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    • pp.167-198
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    • 2022
  • Ten species of copepods, including nine new species, associated with compound ascidians are recorded from Korean waters. Nine new species can be characterized by their major diagnostic features, as follows: Botryllophilus pentamerus n. sp. by having a five-annulated abdomen; B. paucisetatus n. sp. by the presence of four and three setae on the exopods of right and left leg 1, respectively; Haplostoma quadridens n. sp. by the presence of four lobes on the labrum and one seta plus four spines on the exopods of legs 1-4; H. paucidens n. sp. by the presence of only two spines on the distal segment of the antenna; Enterocola horridus n. sp. by having five setae on the antenna, no seta on the caudal ramus, and two setae on leg 5; E. longicaudatus n. sp. by having long caudal rami which are more than three times as long as wide; Thoracodelphys bisetata n. sp. by the presence of only two setae on the basis of the maxillule; T. cerasta n. sp. by the presence of a large, horn-like process on the distal margin of the basis of leg 2; and Unimeria hirsuta n. sp. by having three setae on the terminal segment of the maxilla. Zygomolgus didemni (Gotto, 1956) previously known only from European waters is reported from Korean waters, with a redescription and illustrations.

Cyclopoid Copepods (Crustacea) Associated with Polychaetes from Korea, with Descriptions of Two New Genera and Ten New Species

  • Jimin Lee;Il-Hoi Kim
    • Animal Systematics, Evolution and Diversity
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    • v.40 no.3
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    • pp.167-210
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    • 2024
  • Twelve species of cyclopoid copepods, including two new genera and ten new species, associated with polychaetes are described from Korea. The new taxa comprise Petalocopus planus n. gen. n. sp. in the Clausidiidae, Rhodinicola exilis n. sp. in the Clausiidae, Gastrodelphys vadicola n. sp. in the Gastrodelphyidae, Polyankylis estivalis n. sp. in the Polyankyliidae, Pentakela cylindrica n. gen. n. sp., Pseudanthessius oporinus n. sp. and P. petilus n. sp. in the Pseudanthessiidae, and Myxomolgus buanensis n. sp., M. dokdoicus n. sp. and Serpuliphilus antennatus n. sp. in the Sabelliphilidae. As characteristic features of the new genera, Petalocopus n. gen. has a pair of pedunculate suckers on the terminal segment of the antenna and an enlarged posterodistal seta on the second segment of antennule, and Pentakela n. gen. has five strong hooks on distal segments of antenna, three-segmented exopod and two-segmented endopod in legs 1 and 2, and one-segmented exopod and a lobate, rudimentary endopod in legs 3 and 4. The male of Maxilliclausia propria Kim, 2014 of the Clausiidae is recorded for the first time. Nereicola ovata Keferstein, 1863 known previously only in European waters is redescribed based on a newly collected female specimen from Korea.

Persistent Trigeminal Artery Variant Detected by Conventional Angiography and Magnetic Resonance Angiography -Incidence and Clinical Significance-

  • Rhee, Sun-Joo;Kim, Myoung-Soo;Lee, Chae-Heuck;Lee, Ghi-Jai
    • Journal of Korean Neurosurgical Society
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    • v.42 no.6
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    • pp.446-449
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    • 2007
  • Objective : Persistent trigeminal artery variant (PTAV) is an anastomosis between the internal carotid artery (ICA) and the cerebellar artery without any interposing basilar artery segment. We discuss its probable embryological origin and emphasize clinical implications. Methods : Retrospectively 1250 conventional cerebral angiograms and 2947 cranial magnetic resonance angiographies (MRAs) were evaluated for the patients with PTAV. Results : Five patients (four men and one woman, 23 to 76 years of age, median age 65 years) had a PTAV. Three patients who underwent MRA had a PTAV (3/2947=0.1%). Four of the patients who underwent cerebral angiography had a PTAV (4/1250=0.32%). Two of 143 patients who underwent both conventional angiography and cranial MRA showed PTAV. The PTAV was an incidental finding in all five patients. The PTAV originated from the cavernous segment of the left ICA in four patients and from the cavernous segment of the right ICA in one patient. The terminal branch of the PTAV was the anterior inferior cerebellar artery (AICA) and superior cerebellar artery (SCA) in two patients and the AICA only in the other three patients. Conclusion : Neurosurgeons should be aware of possible presence of PTAV. Manipulation of this vessel during a surgical approach to the parasellar region and percutaneous gasserian ganglion procedure may result in hemorrhage or ischemia.