• Title/Summary/Keyword: terminal density

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Molecular Cloning and Sequencing of the Bacillus subtilis cdd Gene Encoding Dooxycytindine-Cytidine Deaminase

  • Song, Bang-Ho;Neuhard, Jan
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1986.12a
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    • pp.512.1-512
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    • 1986
  • The cdd gene of Bacillus subtilis, encoding the deoxycytidinecytidine deaminase of pyrimidine nucleotide biosynthesis has been cloned into the EcoRl site of pBR322. The recombinant plasmid, pSol, promoted the synthesis of 100-140 fold elevated levels of the enzyme. A comparison of the polypeptides encoded by cdd complementing and non-complementing plasmids in the mini cell showed the gene product to have a molecular mass of approximately 14 kDa. The nucleotide sequence of the gene and 460 base pairs upstream from the coding region was determined. An open-reading frame, encoding a protein with a calculated molecular mass of 14337 Da, was deduced to be the coding region for cdd. However, the enzyme has an apparent molecular mass of 54 kDa as determined by gel filteration, whereas sucrose density gradient centrifugation shows 58 kDa. It means that the enzyme could be forming a tetramer in a physiological state. About 28 amino acids of the N-tetramer in a physiological state. About 28 amino acids of the N-terminal presumably form a signal for membrane translocation and six cystein residues are contained in the structure. S1 nuclease mapping indicated that transcription of cdd is initiated 17 base pairs upstream from the translational start. The structural characterization of the odd gene was performed.

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Signal Transduction-related Gene Expression Analysis in MCF-7 followed by $\gamma$-radiation (MCF-7 세포주에서$\gamma$선에 의한 세포신호 전달 관련 유전자의 발현 양상의 분석)

  • 박지윤;황창일;박웅양;김진규;채영규
    • Korean Journal of Environmental Biology
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    • v.21 no.1
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    • pp.52-55
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    • 2003
  • There is considerable evidence that ionizing radiation (IR) mediates checkpoint control, repair and cell death. In this study, we have used a high density microarray hybridization approach to characterize the transcriptional response of human breast carcinoma MCF-7 cell line to ${\gamma}$-radiation, such as 4 Gy 4 hr, 8 Gy 4 hr, and 8 Gy 12 hr. We found that exposure to ${\gamma}$-ray alters by at least a $log_2$ factor of 1.0 the expression of 115 known genes. Of the 66 genes affected by ${\gamma}$-radiation, 49 are down-regulated. In our results, the cellular response to irradiation includes induction of the c-jun and EGR1 early response genes. The present work has examined potential cytoplasmic signaling cascades that transduce IR-induced signals to the nucleus. 40S ribosomal protein s6 kinase modulates the activities of the mitogen activated protein kinase (MAPK) and c-Jun $NH_2$-terminal kinase (JNK1) cascades in human monocytic leukemia (U937/pREP4) cells. 14-3-3 family members are dimeric phosphoserine -binding proteins that participate in signal transduction and checkpoint control pathways.

An Satellite Communication Wireless Package System Using Analysis of Channel Interference between ISM band Systems (ISM 대역 시스템간 채널 간섭 분석을 통한 위성 통신 무선 패키지시스템 적용)

  • Ko, Hojeong;Cha, Jaesang
    • Journal of Satellite, Information and Communications
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    • v.10 no.3
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    • pp.78-83
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    • 2015
  • In this paper, when WLAN repeater of satellite communication package system as a novel wireless disaster communication network connected to LTE D2D mobile terminal, we analyzed radio channel interference from WLAN and WPAN system of adjacent same ISM band using Monte-Carlo method. In this study, WLAN cell radius was determined using Extended Hata Model considering practical environment, and simulated physical protection distance and density in the dense-mode to minimize interference from WLAN, Bluetooth, and ZigBee. Simulation results, WLAN repeater can be operated with 15 WLAN interferer over 130m distance, 23 Bluetooth interferer over 100m distance, and with 62 ZigBee interferer over 83m distance.

A Proteomic Screen for Presynaptic Terminal N-type Calcium Channel (CaV2.2) Binding Partners

  • Khanna, Rajesh;Zougman, Alexandre;Stanley, Elise F.
    • BMB Reports
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    • v.40 no.3
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    • pp.302-314
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    • 2007
  • N type calcium channels (CaV2.2) play a key role in the gating of transmitter release at presynaptic nerve terminals. These channels are generally regarded as parts of a multimolecular complex that can modulate their open probability and ensure their location near the vesicle docking and fusion sites. However, the proteins that comprise this component remain poorly characterized. We have carried out the first open screen of presynaptic CaV2.2 complex members by an antibody-mediated capture of the channel from purified rat brain synaptosome lysate followed by mass spectroscopy. 589 unique peptides resulted in a high confidence match of 104 total proteins and 40 synaptosome proteome proteins. This screen identified several known CaV2.2 interacting proteins including syntaxin 1, VAMP, protein phosphatase 2A, $G_{o\alpha}$, G$\beta$ and spectrin and also a number of novel proteins, including clathrin, adaptin, dynamin, dynein, NSF and actin. The unexpected proteins were classified within a number of functional classes that include exocytosis, endocytosis, cytoplasmic matrix, modulators, chaperones, and cell-signaling molecules and this list was contrasted to previous reports that catalogue the synaptosome proteome. The failure to detect any postsynaptic density proteins suggests that the channel itself does not exhibit stable trans-synaptic attachments. Our results suggest that the channel is anchored to a cytoplasmic matrix related to the previously described particle web.

Study of a portable and vehicle carryable realtime video transmitting system on expressways (고속도로 현장에서 휴대형.차량탑재형 실시간 동영상 전송 시스템에 관한 연구)

  • Kim, Jong-Heon;Lee, Dong-Soo
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.11 no.2
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    • pp.111-116
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    • 2012
  • In this paper, we fabricate a WiFi based video transmitting equipment and establish a video transmitting system for realtime transmitting of accident scene on expressways. The transmitting equipment is based on the H.264 video codec and embed WiFi module. The transmitting equipment can transmit video and also meta data such as location positioning through the Wibro network using a Wibro terminal. From test results, it is clear that high density videos were transmitted while the vehicle carried by the video equipment was traveling with velocity of 80 km/h.

The Design and Fabrication of SRAM Modules Surface Mounted on Multilayer Borads (다층 기판 위에 표면실장된 SRAM 모듈 설계 제작)

  • Kim, Chang-Yeon;Jee, Yong
    • Journal of the Korean Institute of Telematics and Electronics A
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    • v.32A no.3
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    • pp.89-99
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    • 1995
  • In this paper, we ecamined the effect that MCM-L technique influencess on the design and fabrication of multichip memory modules in increasing the packing desity of memory capacity and maximizing its electrical characteristics. For that purpose, we examined the effective methods of reducing the area of module layout and the wiring length with the variation of chip allocation and the number of wiring layers. We fabricated a 256K${\times}$8bit SRAM module with eight 32K${\times}$8bit SRAM chips. The routing experiment showed that we could optimize the area of module layout and wiring length by placing chips in a row, arranging module I/O pads parallel to chip I/O pads, and equalizing the number of terminal sides of module I/O's to that of chip I/O's. The routing was optimized when we used three wire layers in case of one sided chip mounting or five wire layers in case of double sided chip mounting. The fabricated modules showed 18.9 cm/cm$^{2}$ in wiring density, 65 % in substrate occupancy efficiency, and module substrate and functionally tested to find out the module working perfectly.

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A study on the structural changes and the TSC characteristics of epoxy composites cured with acid-anhydride (산무수물 경화된 에폭시 복합체의 구조변화와 TSC특성에 관한 연구)

  • 왕종배;이준웅
    • Electrical & Electronic Materials
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    • v.7 no.1
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    • pp.32-41
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    • 1994
  • In this study, the TSC spectroscopy has been applied to investigate the influence of structural change due to a process of curing reaction on the electrical properties of epoxy composites cured with acid-anhydride. Five TSC peaks appeared in -160-250[.deg.C]: in the low temperature region below glass transition temperature(T$\_$g/), three relaxation mode peaks due to action of side chains, substitution group or terminal groups have been observed, a peak associated with T$\_$g/, appeared in 110[.deg. C] and p peak due to ionic space charges located in 150[.deg.C]. Each peak was separated into elementary peaks by the partial polarization procedure, and the distribution of activation energy and relaxation time were analized to clearify the origin of each peak. Also, overaboundantly added hardener separated a .betha. peak near 10[.deg. C] into two peaks of .betha.$\_$1/(10.deg. C) and .betha.$\_$2/(20.deg. C) according to increasement of forming field, and the separated hardener was oxidated thermally with increasing surrounding temperatures. The expansion of the free volume need in molecular motion and the reduction of the structural packing density through thermal oxidation process increased TSC between .alpha. peak and .betha. peak and decreased T$\_$g/.

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A Self-Consistent Semi-Analytical Model for AlGaAs/InGaAs PMHEMTs

  • Abdel Aziz, M.;El-Banna, M.;El-Sayed, M.
    • JSTS:Journal of Semiconductor Technology and Science
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    • v.2 no.1
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    • pp.59-69
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    • 2002
  • A semi-analytical model based on exact numerical analysis of the 2DEG channel in pseudo-morphic HEMT (PMHEMT) is presented. The exactness of the model stems from solving both Schrodinger's wave equation and Poisson's equation simultaneously and self-consistently. The analytical modeling of the device terminal characteristics in relation to the charge control model has allowed a best fit with the geometrical and structural parameters of the device. The numerically obtained data for the charge control of the channel are best fitted to analytical expressions which render the problem analytical. The obtained good agreement between experimental and modeled current/voltage characteristics and small signal parameters has confirmed the validity of the model over a wide range of biasing voltages. The model has been used to compare both the performance and characteristics of a PMHEMT with a competetive HEMT. The comparison between the two devices has been made in terms of 2DEG density, transfer characteristics, transconductance, gate capacitance and unity current gain cut-off frequency. The results show that PMHEMT outperforms the conventional HEMT in all considered parameters.

A study on Ar/CF4 Magnetized Inductively Coupled Plasma Using Fluid Simulation (유체시뮬레이션을 통한 Ar/CF4 자화유도결합 플라즈마의 특성 연구)

  • Kim, Yun-Gi;Son, Eui-Jeong;Wi, Sung-Suk;Kim, Dong-Hyun;Lee, Ho-Jun
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.64 no.4
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    • pp.560-566
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    • 2015
  • The self-consistent simulation based on the drift-diffusion approximation with anisotropic transport coefficients was performed. The RHCP-wave propagation was observed in MICP and this wave was refracted toward the high-density region. The calculated impedance seen from the antenna terminal shows that resistance component of MICP is a higher than that of ordinary ICP. Because of a higher resistance, the power transfer efficiency was improved to 95%. This property is practically important for large-size, low-pressure plasma sources because high resistance corresponds to high power-transfer efficiency and stable impedance matching characteristics.

Performance Analysis of Dual-Hop Cooperative Transmission with Best Relay Selection in a Rayleigh Fading Channel

  • Nessa, Ahasanun;Lee, Woo-Yong;Kim, Yong-Sun;Kwak, Kyung-Sup
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.34 no.7A
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    • pp.530-539
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    • 2009
  • Wireless Relaying is a promising solutions to overcome the channel impairments and provides high data rate coverage that appear for beyond 3G mobile communications. In this paper we present end to end BER performance of dual hop wireless communication systems equipped with multiple Decode and Forward relays over Rayleigh fading channel with the best relay selection. We compare the BER performance of the best relay with the BER performance of single relay. We select the best relay based on the end to end channel conditions. We further calculate the outage probability of the best relay. It is shown that the outage probability of the best relay is equivalent to the outage probability when all relays take part in the transmission. We apply Orthogonal Space Time Block coding(OSTBC) at the source terminal. Numerical and simulation results are presented to verify our analysis.