• Title/Summary/Keyword: template threshold

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Detection of Mass on Dense Mammogram (고밀도 유방영상에서 종양의 추출)

  • Yu, Seung-Hwa;No, Seung-Mu;Park, Jong-Won
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.38 no.6
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    • pp.721-734
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    • 2001
  • This paper proposed automated methods for the detection of breast mass. We analysed characteristic of the mass by using the features on mammograms. The homogeneity was used to distinguish mass and abnormal homogeneous tissue from the Cooper's ligament and multiple threshold method was used to deal with the high density candidates. By using the 8-connectivity, the first step candidates were selected. We generated the dualistic images of each candidate in which we regard the gray value as topographic height information. From these candidates, the second candidates were selected by comparing the circularity and the distribution rates. The final detection was done with the method in which we generated the template of each candidate and compared each other. From these methods, we grade the order from the candidate. We applied the algorithm to the 136 mammograms and compared to the radiologist's outlines of the leisions. The detection resulted that the sensitivity of the proposed methods was 93.38% and 97.63% FP(False positive) which we can segmented mass in the first grade in the 124 cases.

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Properties of the RF Sputter Deposited n-ZnO Thin-Film and the n-ZnO/p-GaN heterojunction LED (RF스퍼터링법으로 성장시킨 n-ZnO 박막과 n-ZnO/p-GaN 이종접합 LED의 특성)

  • Shin, Dongwhee;Byun, Changsub;Kim, Seontai
    • Korean Journal of Materials Research
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    • v.23 no.3
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    • pp.161-167
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    • 2013
  • The ZnO thin films were grown on GaN template substrates by RF magnetron sputtering at different RF powers and n-ZnO/p-GaN heterojunction LEDs were fabricated to investigate the effect of the RF power on the characteristics of the n-ZnO/p-GaN LEDs. For the growth of the ZnO thin films, the substrate temperature was kept constant at $200^{\circ}C$ and the RF power was varied within the range of 200 to 500W at different growth times to deposit films of 100 nm thick. The electrical, optical and structural properties of ZnO thin films were investigated by ellipsometry, X-ray diffraction (XRD), atomic force microscopy (AFM), photoluminescence (PL) and by assessing the Hall effect. The characteristics of the n-ZnO/p-GaN LEDs were evaluated by current-voltage (I-V) and electroluminescence (EL) measurements. ZnO thin films were grown with a preferred c-axis orientation along the (0002) plane. The XRD peaks shifted to low angles and the surface roughness became non-uniform with an increase in the RF power. Also, the PL emission peak was red-shifted. The carrier density and the mobility decreased with the RF power. For the n-ZnO/p-GaN LED, the forward current at 20 V decreased and the threshold voltage increased with the RF power. The EL emission peak was observed at approximately 435 nm and the luminescence intensity decreased. Consequently, the crystallinity of the ZnO thin films grown with RF sputtering powers were improved. However, excess Zn affected the structural, electrical and optical properties of the ZnO thin films when the optimal RF power was exceeded. This excess RF power will degrade the characteristics of light emitting devices.

Use of Real-Time PCR and Internal Standard Addition Method for Identifying Mixed Ratio of Chicken Meat in Sausages (Real-Time PCR과 Internal Standard Addition법을 이용한 돼지고기 소시지에 혼합된 닭고기의 정량)

  • Lee, Namrye;Joo, Jae-Young;Yeo, Yong-Heon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.46 no.9
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    • pp.1097-1105
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    • 2017
  • This study examined how much chicken meat was in sausage made with pork. Both real-time polymerase chain reaction (PCR) and internal standard addition were used. Fifty ng of chicken DNA was added to the sausages as an internal standard. The addition of standard DNA increased the amplification efficiency of PCR and confirmed the possibility of quantitative analysis. A QIAamp DNA Micro Kit was used to improve the DNA recovery and amplification efficiency. The density of template DNA and primer were suitable for $3.0{\sim}5.0{\mu}L$ and $0.5{\mu}L$, respectively. Each DNA of pig and chicken was diluted in 10-fold from steps 50 ng to 0.05 ng. The detection limit of both pig and chicken meat was more than 0.05 ng and the correlation coefficient of the standard curve was at least 0.98. The result of the quantitative analysis after heat treatment of 3 samples of pigs and chickens mixed at 70:30 showed a 5.7% difference (64.3:35.7) between the expected value and measured value. The quantitative value was changed by affecting the DNA according to the heat treatment ($70^{\circ}C$, 10 min). An analysis of the pork and chicken content in sausages showed that it was difficult to detect chicken meat and the quantitative value of DNA according to the Ct value was very low. On the other hand, when adding standard material (50 ng of chicken DNA) to the sausages, the Ct value decreased gradually with increasing chicken mixing ratio. Thus, the mixing ratio of chicken in sausages could be estimated.

Genetic Authentication of Cynanchi Wilfordii Radix and Cynanchi Auriculati Radix by Using Conventional-PCR and Real-time PCR (Conventional-PCR 및 Real-time PCR을 이용한 백수오와 이엽우피소의 유전자 종감별 시험법 비교)

  • Ryu, Hoe Jin;Kim, Ae Kyung;Kim, Sung Dan;Jung, Sam Joo;Jang, Jung Im;Lee, Hee Jin;Lee, Jung Mi;Yu, In Sil;Jung, Kweon
    • Korean Journal of Pharmacognosy
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    • v.49 no.1
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    • pp.55-64
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    • 2018
  • Recently, it has been a big issue to distinguish the dried roots of Cynanchum wilfordii and C. auriculatum in health functional food market. The original plant species of Cynanchi Wilfordii Radix belong to the Asclepiadaceae family is differentially described in the national pharmacopoeia of Korea, China and Japan. Owing to the morphological similarities of the dried roots of this plant to those of C. auriculatum, which is often misidentified in Korean herbal medicine marketplace, distinguishing these two species is exceedingly difficult. The purpose of this study was to compare the conventional-PCR with the real-time PCR for detection of C. wilfordii and C. auriculatum DNA. We also tried to realize a quantitative real-time PCR assay using species-specific matK primers, which allowed us to estimate the ratio of C. willfordii and C. auriculatum using varying ratios of mixed genomic DNA template from the two species. The differentiation of intentional and unintentional mixture in this study would be applied to food safety management and can be helpful for protection of consumer's right and cultivators.

The Development of Image Processing System Using Area Camera for Feeding Lumber (영역카메라를 이용한 이송중인 제재목의 화상처리시스템 개발)

  • Kim, Byung Nam;Lee, Hyoung Woo;Kim, Kwang Mo
    • Journal of the Korean Wood Science and Technology
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    • v.37 no.1
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    • pp.37-47
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    • 2009
  • For the inspection of wood, machine vision is the most common automated inspection method used at present. It is required to sort wood products by grade and to locate surface defects prior to cut-up. Many different sensing methods have been applied to inspection of wood including optical, ultrasonic, X-ray sensing in the wood industry. Nowadays the scanning system mainly employs CCD line-scan camera to meet the needs of accurate detection of lumber defects and real-time image processing. But this system needs exact feeding system and low deviation of lumber thickness. In this study low cost CCD area sensor was used for the development of image processing system for lumber being fed. When domestic red pine being fed on the conveyer belt, lumber images of irregular term of captured area were acquired because belt conveyor slipped between belt and roller. To overcome incorrect image merging by the unstable feeding speed of belt conveyor, it was applied template matching algorithm which was a measure of the similarity between the pattern of current image and the next one. Feeding the lumber over 13.8 m/min, general area sensor generates unreadable image pattern by the motion blur. The red channel of RGB filter showed a good performance for removing background of the green conveyor belt from merged image. Threshold value reduction method that was a image-based thresholding algorithm performed well for knot detection.