• Journal of Veterinary Science
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• v.25 no.3
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• pp.35.1-35.13
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• 2024

Importance: Experimental autoimmune encephalomyelitis (EAE) is an animal model of multiple sclerosis characterized by inflammation within the central nervous system. However, inflammation in non-neuronal tissues, including the lungs, has not been fully evaluated. Objective: This study evaluated the inflammatory response in lungs of EAE mice by immunohistochemistry and histochemistry. Methods: Eight adult C57BL/6 mice were injected with myelin oligodendrocyte glycoprotein_35-55 to induce the EAE. Lungs and spinal cords were sampled from the experimental mice at the time of sacrifice and used for the western blotting, histochemistry, and immunohistochemistry. Results: Histopathological examination revealed inflammatory lesions in the lungs of EAE mice, characterized by infiltration of myeloperoxidase (MPO)- and galectin-3-positive cells, as determined by immunohistochemistry. Increased numbers of collagen fibers in the lungs of EAE mice were confirmed by histopathological analysis. Western blotting revealed significantly elevated level of osteopontin (OPN), cluster of differentiation 44 (CD44), MPO and galectin-3 in the lungs of EAE mice compared with normal controls (p < 0.05). Immunohistochemical analysis revealed both OPN and CD44 in ionized calcium-binding adapter molecule 1-positive macrophages within the lungs of EAE mice. Conclusions and Relevance: Taken together, these findings suggest that the increased OPN level in lungs of EAE mice led to inflammation; concurrent increases in proinflammatory factors (OPN and galectin-3) caused pulmonary impairment.

• Biotechnology and Bioprocess Engineering:BBE
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• v.7 no.3
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• pp.155-162
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• 2002

The cyclic amidohydrolase family enzymes, which include allantoinase, dihydroorotase, dihydropyrimidinase and (phenyl)hydantoinase, are metal-dependent hydrolases and play a crucial role in the metabolism of purine and pyrimidine in vivo. Each enzyme has been independently characterized, and thus well documented, but studies on the higher structural traits shared by members of this enzyme family are rare due to the lack of comparative study. Here, we report upon the expression in E. coli cells of maltose-binding protein (MBP)- and glutathione S-transferase (GST)-fused cyclic amidohydrolase family enzymes, facilitating also for both simple purification and high-level expression. Interestingly, the native quaternary structure of each enzyme was maintained even when fused with MBP and GST. We also found that in fusion proteins the favorable biochemical properties of family enzymes such as, their optimal pHs, specific activities and kinetic properties were conserved compared to the native enzymes. In addition, MBP-fused enzymes showed remarkable folding ability in-vitro. Our findings, therefore, suggest that a previously unrecognized trait of this family, namely the ability to functional fusion with some other protein but yet to retain innate properties, is conserved. We described here the structural and evolutionary implications of the properties in this family enzyme.

• Geomechanics and Engineering
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• v.14 no.6
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• pp.563-570
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• 2018

Ballast layer has an important role in vertical stiffness and stability of railway track. In most of the Middle East countries and some of the Asian ones, significant parts of railway lines pass through desert areas where the track (particularly ballast layer) is contaminated with sands. Despite considerable number of derailments reported in the sand contaminated tracks, there is a lack of sufficient studies on the influences of sand contamination on the ballast vertical stiffness as the main indicator of track stability. Addressing this limitation, the effects of sand contamination on the mechanical behavior of ballast were experimentally investigated. For this purpose, laboratory tests (plate load test) on ballast samples with different levels of sand contamination were carried out. The results obtained were analyzed leading to derive mathematical expressions for the strain modulus ($E_V$) as a function of the ballast level of contamination. The $E_V$ was used as an index for evaluation of the load-deformation characteristics and bearing capacity of track substructure. The critical limit of sand contamination, after which the $E_V$ of the ballast reduces drastically, was obtained. It was shown that the obtained research results improve the current track maintenance approach by providing key guides for the optimization of ballast maintenance planning (the timing of ballast cleaning or renewal).

• Journal of Digital Convergence
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• v.10 no.1
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• pp.135-145
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• 2012

We are now observing that the information technology environments are rapidly changing toward smart and soft power computing. The main purpose of this paper is to investigate national-level information strategies and to provide suggestions for sustained growth of information technology industry which have played a significant role in upgrading national wealth and brand. In this context we look into the governance structures of the current government, delineate problematic sources, and derive remedial solutions, if any.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2011.07a
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• pp.365-368
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• 2011

본 논문에서는 시청자의 색상 특성을 고려하여 양안식 영상에서의 색상 불일치를 예측하는 방법을 제안했다. 먼저 색상 불일치 예측을 위한 주관적 평가로 색상 민감도 평가를 수행했다. 기본 세 채널 색 R, G, B에 대해 각각의 색상의 오차 범위를 결정한 후, 이를 기반으로 관심도와 복잡도를 평가하여 보다 세밀한 주관적 평가를 수행했다. 실험 결과를 통해 제안한 방법이 기존의 색상의 품질을 평가하기 위해 사용하는 유클리디안 색 거리 계산 방법과 US & Canadian Government Printing Offices의 품질 수준 규격(Qulaity Level Specifications)을 이용한 방법보다 효율적으로 색상 불일치 여부를 평가함을 확인했다.

• Journal of Computing Science and Engineering
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• v.7 no.3
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• pp.168-176
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• 2013

Long-term evolution (LTE) is emerging as a major candidate for 4G cellular networks to satisfy the increasing demands for mobile broadband services, particularly multimedia delivery. Multiple-input multiple-output (MIMO) technology combined with orthogonal frequency division multiple access and more efficient modulation/coding schemes (MCS) are key physical layer technologies in LTE networks. However, in order to fully utilize the benefits of the advances in physical layer technologies, the MIMO configuration and MCS need to be dynamically adjusted to derive the promised gains of 4G at the application level. This paper provides a performance evaluation of video traffic with variations in the physical layer transmission parameters to suit the varying channel conditions. A quantitative analysis is provided using the perceived video quality as a video quality measure (evaluated using no-reference blocking and blurring metrics), as well as transmission delay. Experiments are performed to measure the performance with changes in modulation and code rates in poor and good channel conditions. We discuss how an adaptive scheme can optimize the performance over a varying channel.

• Journal of the Ergonomics Society of Korea
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• v.33 no.5
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• pp.433-452
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• 2014

Objective: The aim of this study is to give ergonomists the brief summary of the recently published ISO standards on human-system interaction and tips for application of the standards. Background: Standard developers did hard work on developing a standard in a concise manner. But most of standards are often bulky in volume. Readers of the standards are difficult to catch key points from the voluminous contents of standards and intermingle among them. Method: Focused on newly developed display/control technology, this study reviewed the 14 ISO standards on human-system interaction published during 2008-2013 and summarized key points from them. Results: Schematic diagrams and tables concisely illustrated the processes, procedures, dimensions, or best practices recommended by the standards concerning conception, design, and usability testing for consumer products. Conclusion: The standards provided the minimum level of requirements on design and evaluation on the physical input devices, electronic displays, and control interfaces based on the current state of technology. But the minimum requirements specified in the standards nowadays become mandatory ergonomic requirements in global trade world. Application: Ergonomists can take a quick and broad view on international standardization activities on newly developed display/control technology from this summary study.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2009.01a
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• pp.89-93
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• 2009

Embedded systems are becoming more popular as many embedded platforms have become more affordable. It offers a compact solution for many different problems including computer vision applications. Texture classification can be used to solve various problems, and implementing it in embedded platforms will help in deploying these applications into the market. This paper proposes to deploy the texture classification algorithms onto the embedded computer vision (ECV) platform. Two algorithms are compared; grey level co-occurrence matrices (GLCM) and Gabor filters. Experimental results show that raw GLCM on MATLAB could achieves 50ms, being the fastest algorithm on the PC platform. Classification speed achieved on PC and ECV platform, in C, is 43ms and 3708ms respectively. Raw GLCM could achieve only 90.86% accuracy compared to the combination feature (GLCM and Gabor filters) at 91.06% accuracy. Overall, evaluating all results in terms of classification speed and accuracy, raw GLCM is more suitable to be implemented onto the ECV platform.

• Journal of Welding and Joining
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• v.35 no.3
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• pp.21-27
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• 2017

A soldering temperature of ($235{\pm}3$) $^{\circ}C$ is described in ISO 9455-15 for the copper corrosion test. However, this temperature is not suitable for performing lead-free solder pastes. We evaluated the compatibility of a lead-free solder paste in the experimental conditions of (Liquidus temperature + ($35{\pm}3$)) $^{\circ}C$. Based on the results after a Cu corrosion test, a proper temperature for Pb-free soldering was (melting point+($35{\pm}3$)) $^{\circ}C$. Criteria used to evaluate corrosion due to discoloration of flux residue is described in ISO 9455-15, but a more quantitative evaluation standard is needed. In this study, experimental error level was estimated by analyzing flux residue after a corrosion test for 72, 500 hours of specimens using EDS analysis with acceleration voltage. It was determined that the copper area at the flux residue boundary is suitable for the EDS analysis area.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.24 no.2
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• pp.91-101
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• 2012

A 200 W portable polymer electrolyte membrane fuel cell (PEMFC) system is developed. The PEMFC system consists of an air-cooled fuel cell stack module, a fuel supply subsystem, a power management subsystem, and a control electronics subsystem. The control logic is designed for the stable system operation. The system-level performance evaluation discloses that the present PEMFC system provides a rated power output of 200.5 W at 13.4 V with the maximum balance-of-plant (BOP) efficiency of 72%, and maximum system efficiency based on lower heating value (LHV) is 37% at 120.7 W system power output.