• The Plant Pathology Journal
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• 제16권2호
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• pp.98-104
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• 2000

For the taxonomic evaluaition, 15 strains of the genus Pectobacterium and Erwinia were analyzed for 16S-23S rDNA intergenic spacer regions (ISRs). These species contained two types of ISRs, large and small ISRs. Large ISRs were on the range of 474-569 bp size, and coding transfer $\textrm{RNA}^{11e}$($\textrm{tRNA}^{11e}$) and $\textrm{tRNA}^{Ala}$. Small ISRs were 354-459 bp in length and coding $\textrm{tRNA}^{Glu}$. The sequence variations of two ISRs among species and strains were very high as compared with 16S rRNA gene sequences. By phylogenetic trees on the basis of two ISRs, Pectobacterium ere differentiated into P. carotovorum-P. cactiaidum group and P. chrysanthemi group. However, the taxonomic position of E. cypripedii and E. rhapontici, which were not clear on taxonomic delineation between Pectobacterium and Erwinia, were not clearly resolved on the basis of ISRs.

• Journal of Ecology and Environment
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• 제31권1호
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• pp.75-81
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• 2008

Rates of molecular evolution are known to vary widely among taxonomic groups. A number of studies, examining various taxonomic groups, have indicated that body size is negatively and clutch size is positively correlated with the rates of nucleotide substitutions among vertebrate species. Generally, either smaller body mass or larger clutch size is associated with shorter generation times and higher metabolic rates. However, this generality is subject to ongoing debate, and large-scale comparative studies of species below the Order level are lacking. In this study, phylogenetically independent methods were used to test for relationships between rates of the mitochondrial cytochrome b evolution and a range of life history traits, such as body mass and clutch size in the Order Galliformes. This analysis included data from 67 species of Galliformes birds and 2 outgroup species in Anseriformes. In contrast to previous studies, taxa were limited to within-Order level, not to Class or higher. I found no evidence to support an effect of life history traits on the rate of molecular evolution within the Galliformes. These results suggest that such relationship may be too weak to be observed in comparisons of closely related species or may not be a general pattern that is applicable to all nucleotide sequences or all taxonomic groups.

• International journal of advanced smart convergence
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• 제8권3호
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• pp.115-122
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• 2019

Recent and ongoing discoveries of mycoviruses with new properties demand the development of an appropriate research infrastructure to analyze their evolution and classification. In particular, the discovery of negative-sense single-stranded mycoviruses is worth noting in genome types in which double-stranded RNA virus and positive-sense single-stranded RNA virus were predominant. In addition, some genomic properties of mycoviruses are more interesting because they have been reported to have similarities with the pathogenic virus family that infects humans and animals. Genetic information on mycoviruses continues to accumulate in public repositories; however, these databases have some difficulty reflecting the latest taxonomic information and obtaining specialized data for mycoviruses. Therefore, in this study, we developed a bioinformatics-based pipeline to efficiently utilize this genetic information. We also designed a schema for data processing and database construction and an algorithm to keep taxonomic information of mycoviruses up to date. The pipeline and database (termed 'mycoVDB') presented in this study are expected to serve as useful foundations for improving the accuracy and efficiency of future research on mycoviruses.

• 한국물환경학회지
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• 제39권4호
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• pp.288-301
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• 2023

This study aims to identify benthic macroinvertebrate fauna inhabiting at the mountain valley stream (Mt. Cheonggye) in Korea and the relationship between physical environmental factors and biological indices. Benthic macroinvertebrates were collected at five locations on August 24 and October 14, 2020, and were identified as 4 phyla, 7 classes, 16 orders, 42 families, and 72 species. Dominance ranged from 0.38 to 0.59, diversity 2.81 to 3.75, richness 3.25 to 4.63, evenness 0.65 to 0.84, and %EPT (Ephemeroptera-PlecopteraTrichoptera) richness value 42% to 73%, respectively. All sites were evaluated as a very good status by mostly biological indices based on tolerance of indicator organisms in Korea. As a result of principal component analysis, biological indices are classified into species-level indices and higher cartegory-level indices according to the taxonomic level of the indicator organism considered in each index. As a result of canonical correspondence analysis, it was confirmed that current velocity was a major factor that increased species richness and classified biological indices according to taxonomic category level. Water depth was a major factor related to the community indices, and the deeper the water depth, the lower the diversity and the evenness.

• 한국자원식물학회지
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• 제12권4호
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• pp.324-339
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• 1999

한국산 쓴풀속(용담과) 식물 중 남한에 분포하는 5분류군과 변이체라 생각되는 1분류군, 총 6분류군에 대하여 외부형태학적 형질을 재검토하였고 판별분석(44개의 양적형질)을 수행하여 절과 종간의 한계를 분명히 하여 종 동정의 어려움을 해결하고 분류학적 위치를 설정하고자 본 연구를 수행하였다. 또한 북한에 분포하는 1분류군은 동경대학의 소장품을 관찰하여 분류군의 기재에만 사용하였다. 조사결과, 밀선의 전체적 형태, 화관과 꽃받침의 열편수에 의해 2개 절로 구분되었으며 경생엽의 형태와 화관의 색과 무의에 의해 종간 구분이 가능하였다. 변이체라고 생각되었던 큰잎쓴풀(화관 열편에 자색 점무의를 갖는 분류군) A형은 자색 점 이외의 형질들에서는 전혀 차이를 찾아볼 수 없어 모종의 변이 폭에 넣는 것이 타당하다고 생각된다. 판별분석에 의해 쓴풀속의 식물들을 구별하는 중요한 양적형질은 화관에 관여하는 형질들임이 밝혀졌다. 종자의 형태와 종피의 형질은 종이상을 구별하는 매우 유용한 형질로 밝혀졌다. 따라서 한국산 쓴풀속 식물들에 대한 종 동정의 어려움을 해결하였으며, 속내의 분류학적 위치를 명확히 하였다.

• Mycobiology
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• 제48권6호
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• pp.476-483
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• 2020

The genus Pholiota (Strophariaceae, Basidiomycota) is made up of wood-rotting saprotrophic mushrooms characterized by a yellow or brown pileus with scales and/or slimy, and by a brownish smooth spore with a germ pore. However, these features are not enough to distinguish its species, or separate the genus Pholiota from other brown-spored wood-rotting genera such as Hypholoma and Stropharia. Although internal transcribed spacer (ITS) sequencebased identification has improved identification accuracy for species of Pholiota, most Pholiota species in Korea are reported based on morphological features. To evaluate the taxonomy of Pholiota species, we investigated 62 specimens collected from 1999 to 2019 in Korea using ITS sequence analysis and morphological observation. Twelve of the 16 recorded Pholiota species in Korea were identified. While eight species were clearly separated, the ITS analysis did not distinguish three in the Pholiota adiposa complex. Therefore, further investigation is required to distinguish these three species. ITS sequences deposited in GenBank confirm that P. highlandensis exists in Korea. The presence of the other four Pholiota species could not be confirmed through specimens or sequence information in GenBank. A taxonomic key and the ITS sequence data for Korean Pholiota species are included and can be good baselines for further research on Pholiota taxonomy and diversity.

• 식물분류학회지
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• 제36권3호
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• pp.189-207
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• 2006

로젯사철란(G. rosulacea Y. Lee)은 애기사철란과 유사하나 로젯트형의 잎, 짧은 땅속줄기와 서식지 등의 특징에 의해 한국산 사철란속(Goodyera R. Br.) 내의 신종으로 기재된 바 있다. 로젯사철란의 분류군의 실체와 근연종간의 유연관계를 파악하기 위하여 군외군을 포함한 24개의 사철난속 식물을 대상으로 핵 리보좀(ribosomal)의 DNA internal transcribed spacer와 엽록체 DNA의 trnL 구간의 염기서열을 분석하였다. 분류군의 실체와 근연종간의 유연관계는 정렬된 염기서열을 바탕으로 최대절약분석(Maximum parsimony analysis)와 근연결합법(Neibour Joining method)에 의한 계통수 및 고유 표지유전자 여부로 추정하였다. 분석 결과 로젯사철란은 다수의 고유한 표지유전자를 가지며, ITS와 trnL 계통수에서 모두 단계통군을 형성하였다. 로젯사철란과 한국산 사철란속 내 각 분류군간의 유전적 거리(pairwise distance)는 ITS에서 3.49-6.68, trnL에서 5.05-9.53으로서 독립된 종으로 간주하기에 무리가 없었다. 따라서 분자적 결과는 로젯사철란을 사철란속내 독립된 종으로 처리하는 것을 지지하였다. 계통수에서 로젯사철란은 형태적으로 유사한 애기사철란(G. repens)과 동일한 분계조를 형성하였고, 유전적 거리도 조사한 분류군들 중 가장 낮은 값을 나타냈으므로 가장 가까운 근연분류군임을 나타내었다.

• 농업과학연구
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• 제16권2호
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• pp.163-168
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• 1989

분리(分離)된 저영양세균(低營養細菌)중, 질소고정균(窒素固定菌)과 메타놀 이용균(利用菌)의 대부분이 분류학상(分類學上)새로운 균(菌)으로서 특정지을 수 있었다. 분리된 질소고정균의 경우 형태적 생리적인면에서 Azospirillum에 대응하는 균이라 생각되어 졌으나, 균체지방산조성 및 키논종(種)에 있어서 명확한 차이점을 보여 Azospirillum 속의 새로운 균주로써 추정되었다. 메타놀이용균중 부속기갖는 세포로된 균주들은 prosthecate bacteria(Hyponycrobium sp.)에 대응하는 균이라고 추정되어졌다. 그이외의 regular irregular rods를 나타내는 메타놀이용균에 대해서는 균체지방산조성면에 있어서 서로 다르다는 것이 주목되어져 균체지방산조서의 분석결과(分析結果)를 갖고 화학분류(化學分類)의 측면에서 재검토한 결과 유사도(類似度) 85-90% level에서 8개 cluster로 나뉘어졌다. 일부, cluster에 속하는 균주는 Pseudomonas sp.와 유사한 균으로 추정되었으며, 그 외의 균주들에 대해서는 분류학적 검토가 더욱더 필요로 한다.

• Journal of Plant Biotechnology
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• 제49권2호
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• pp.139-144
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• 2022

The genus Prunus (family: Rosaceae) consists of over 400 plant species and exhibits vast biodiversity worldwide. Given the wide distribution of this genus, its taxonomic classification is important. Anatomical characteristics are conserved and stable and can therefore be used as an important tool for the taxonomic characterization of plants. Therefore, this study aimed to assess and document the anatomical characteristics of the leaf, stem, and seed of P. africana using micrographs and photographs for possible use in the identification, quality control, and phylogenetic analysis of the species. The anatomical sections of a young stem revealed a cortex consisting of isodiametric parenchyma cells, druse crystals, primary vascular bundles, and pith. The mature stem bark majorly consisted of the rhytidome, with the periderm densely arranged in multiple layers; a cluster of stone cells; and sclerenchyma. The leaf sections were hypostomatic, with stomata sizes ranging from 18.90-(22.34)-26.90 × 15.41-(18.40)-21.22 ㎛. The leaf sections showed the presence of characteristic druse crystals, vascular bundles, and mesophyll layers. The pericarp contained the epicarp, mesocarp, and endocarp, with their thickness being approximately 350-400, 300-350, and 30-50 ㎛, respectively. In addition, it contained a seed testa with a thickness of approximately 50-60 ㎛. The morphological and anatomical characteristics observed in P. africana leaves, stems, and seeds in this study could serve as useful data for the taxonomic identification of this species.

• 환경생물
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• 제23권4호
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• pp.335-342
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• 2005

We cloned seven genes encoding chitin synthases (CHSs) by PCR amplification from genomic DNAs of four strains of the genus Sporobolomyces and of Bensingtonia subrosea using degenerated primers based on conserved regions of the CHS genes. Though amino acid sequences of these genes were shown similar as 176 to 189 amino acids except SgCHS2, DNA sequences were different in size, which was due to various introns present in seven fragments. Alignment and phylogenetic analysis of their deduced amino acid sequences together with the reported CHS genes of basidiomycetes separated the sequences into classes I, II and III. This analysis also permitted the classification of isolated CHSs; SgCHS1 belongs to class I, BsCHS1, SaCHS1, SgCHS2, SpgCHS1, and SsCHS1 belong to class II, and BsCHS2 belongs to class III. The deduced amino acid sequences involving in class II that were discovered from five strains were also compared with those of other basidiomycetes by CLUSTAL X program. The bootstrap analysis and phylogenetic tree by neighbor-joining method revealed the taxonomic and evolutionary position for four strains of the genus Sporobolomyces and for Bensingtonia subrosea which agreed with the previous classification. The results clearly showed that CHS fragments could be used as a valuable key for the molecular taxonomic and phylogenetic studies of basidiomycetes.