The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Phylogenetic Analysis of Pectobacterium Species Using the 16S-23S rRNA Intergenic Spacer Regions

  • Kwon, Soon-Wo (Division of Molecular Genetics, National Institute of Agricultural Science, Technology) ;
  • Cheun, Meung-Sook (Division of Molecular Genetics, National Institute of Agricultural Science, Technology) ;
  • Kim, Sang-Hee (Division of Molecular Genetics, National Institute of Agricultural Science, Technology) ;
  • Lim, Chun-Keun (Division of Biological Environment, College of Agriculture, Life Sciences)
  • Published : 2000.04.01
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Abstract

For the taxonomic evaluaition, 15 strains of the genus Pectobacterium and Erwinia were analyzed for 16S-23S rDNA intergenic spacer regions (ISRs). These species contained two types of ISRs, large and small ISRs. Large ISRs were on the range of 474-569 bp size, and coding transfer $\textrm{RNA}^{11e}$($\textrm{tRNA}^{11e}$) and $\textrm{tRNA}^{Ala}$. Small ISRs were 354-459 bp in length and coding $\textrm{tRNA}^{Glu}$. The sequence variations of two ISRs among species and strains were very high as compared with 16S rRNA gene sequences. By phylogenetic trees on the basis of two ISRs, Pectobacterium ere differentiated into P. carotovorum-P. cactiaidum group and P. chrysanthemi group. However, the taxonomic position of E. cypripedii and E. rhapontici, which were not clear on taxonomic delineation between Pectobacterium and Erwinia, were not clearly resolved on the basis of ISRs.

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