• Journal of Animal Environmental Science
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• v.7 no.1
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• pp.21-28
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• 2001

Optimal conditions for utilizing the slaughtered porcine blood and liver for yeast culture and the effects of the yeast cultures on the growth of broiler chicks were investigated. The quantity of yeast cultured for 24hours in the BSG medium containing blood extracts containing 5% glucose and in the LSG medium containing liver extracts containing 5% glucose were higher by 4% and 10%, respectively, than that in the YEPD medium containing 1% yeast extracts, 2% bacto pepton and 2% glucose. Optimal concentrations of ammonium sulfate supplementation to the BSG medium to increase the quantity of yeast cultured for 24 and 48 hours were 100 mM(1.3%) and 50 mM(0.65%), respectively. The optimal pH for yeast culture in BSG medium ranged from 6 to 7. One percent supplementation of either ammonium sulfate or taurine to LSG medium increased the quantity of yeast by 18% and 9%, respectively, compared to no supplementation. The body weight of chicks fed with 2% and 4% yeast culture supplementations cultivated increased at the 4th week by 10%, with relative to no supplementation. The results from this study suggest that the slaughtered porcine blood and liver can be utilized for yeast culture which is used in animal diets.

• Asian-Australasian Journal of Animal Sciences
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• v.17 no.1
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• pp.109-115
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• 2004

Three experiments were conducted to evaluate the effects of taurine (Tau) supplements on broiler growth performance, serum constituents and antibody production. In Exp. 1, 3 day old chicks received a basal diet supplemented with Tau at 0, 0.10, 0.20, 0.30 or 0.40% for 6 weeks. Although dietary Tau supplementing at 0.30 or 0.40% enhanced feed conversion and reduced feed consumption during 0 to 3 weeks (p<0.05), neither serum total cholesterol or anti-Newcastle disease virus (NDV) titer were affected. In Exp. 2, dietary Tau supplement at 0.25-0.75% enhanced feed conversion of broilers during 0 to 3 weeks, but daily gain and feed consumption were not affected. The 0.75% Tau supplement group displayed lower serum total cholesterol at 6 weeks (p<0.05) comparing with the control group but no difference in anti-NDV titers. In Exp. 3, broilers were treated with dietary Tau of 0 or 0.50% combined with low (0/0%), medium (0.18/0.08%), or high (0.36/0.16%) methionine (Met) levels for 6 weeks (0 to 3/3 to 6 weeks). The addition of Met significantly improved daily gain and feed conversion of broilers during 0 to 3 weeks (p<0.01). Dietary Tau interacted significantly with Met on daily gain and feed consumption. Broiler serum amino acids revealed that Met supplements only increased serum Met level, but only serum Tau level was enhanced as given dietary Tau supplementation. The broilers receiving Tau normalized serum triglycerides level by feeding with the low Met diet and tended to display higher anti-NDV titers (p<0.10). The experimental results suggest that the growth response obtained by Tau supplements results partly from interactions with sulfur amino acids. However, the modulation of the broiler lipid metabolism may be responsible for dietary Tau.

• Journal of Animal Science and Technology
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• v.44 no.1
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• pp.45-54
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• 2002

This study was conducted to evaluate the feeding value of ascidian tunic shell the effects of its dietary supplementation on laying performance, egg-yolk pigmentation, egg-shell strength and egg taurine content. A total of 168 brown layers at the age of 29wks in commercial cage were fed for 4 wks with 7 different diets containing ascidian tunic shel1(AST) at varying levels of 0$\sim$5% Dm or 0% AST with 100ppm carophyll red. No differences were found in egg production and weight among the treatments indicating that ascidian tunic shell did not adversely affect the laying performances. Adding the ascidian tunic shell to the diets increased egg-yolk pigmentation compared to the control and resulted in simillar or better effect on egg-yolk pigmentation compared to 100ppm carophyll red. The data suggest that ascidian tunic shell may be used as feed ingredients in layer diet enrichment of egg-yolk pigmentation in the place of carophyll red(chemical pigment). Specific gravity and breaking strength of egg shell were significantly increased by the adding ascidian tunic shell to the diet, suggesting that ascidian tunic shell may be used as feed ingredients for increasing egg shell strength. Also taurine content of egg was significantly increased with increasing supplementation of ascidian tunic shell to the diet(p<0.05). Therefore, ascidian tunic shell may be used as feed ingredients in laying hen diet to improve egg quality such as egg-yolk pigmentation, egg-shell strength and egg taurine enrichment.

• Asian-Australasian Journal of Animal Sciences
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• v.30 no.6
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• pp.828-833
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• 2017

Objective: The study was conducted to evaluate the effects of different methionine (Met) sources on production performance, reproduction performance, egg quality and serum biochemical indices in broiler breeders. Methods: After receiving a basal diet (containing 0.25% Met) for a 2-wk pretreatment period, a total of 360 39-wk-old Lingnan yellow broiler breeders were randomly allocated to four treatments with six replicates each (15 birds per replicate). Breeders were fed with basal diets (control) or diets supplemented with DL-methionine (DLM), DL-2-hydroxy-4-methylthio butytric calcium (MHA-Ca) and coated DL-Met (CME) respectively. Results: The results showed that CME supplementation promoted laying rate and decreased feed-to-egg ratio (F/E) (p<0.05), DLM and MHA-Ca supplementation decreased F/E (p<0.05) when compared with control group. The rate of fertility, hatchability and birthrate were higher (p<0.05) in DLM, MHA-Ca, and CME groups than control group. Compared with control group, CME increased the eggshell thickness (p<0.05); MHA-Ca improved the eggshell thickness, shell ratio and eggshell strength (p<0.05). Results also showed that CME elevated the concentration of total protein in serum (p<0.05); MHA-Ca improved the concentration of calcium in serum (p<0.05). The concentration of serum uric acid in DLM, MHA-Ca, and CME groups was lower than that in control group (p<0.05). Besides, CME had higher concentrations of serum taurine, cysteine and cystanthionine (p<0.05) while MHA-Ca and DLM promoted the serum taurine concentration (p<0.05) compared with control group. Conclusion: Based on the results, it was concluded that Met supplementation could enhance the production and reproduction performance as well as the antioxidant status and egg quality of broiler breeders. In terms of improving the production performance, reproduction performance and antioxidant performance, CME was superior to DLM and MHA-Ca; but in regard to the enhancement of eggshell quality and serum Ca concentration, MHA-Ca was superior to DLM and CME.

• Asian-Australasian Journal of Animal Sciences
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• v.24 no.10
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• pp.1377-1385
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• 2011

This study was conducted to determine the nutritive value of live yeast culture (RumiSacc, Saccharomyces cerevisiae) and to investigate its effects on milk yield, milk composition and some blood parameters in lactating cows. Six multiparous Holstein cows were allocated to two groups of three cows and assigned randomly to one of two diets in a cross-over experiment. Daily 50 g RumiSacc was top dressed at the p.m. feeding for the treatment group. RumiSacc supplied a high protein and energy with high organic matter digestibility values (83.35%) determined by in vitro enzymatic analysis. Yeast culture supplementation significantly increased milk yield, tended to increase fat yield, protein yield and lactose yield of milk. Methylated fatty acid level of 18:3 (n-3) in milk fat was increased by yeast culture supplementation. The concentrations of methionine, phenyalanine, tyrosine, tryptophan and taurine were significantly increased with dietary inclusion of yeast culture. Live yeast culture supplementation did not affect other performance characteristics, milk quality characteristics and blood parameters. As a conclusion live yeast culture (RumiSacc, Saccharomyces cerevisiae) had high nutritive value and positive effects on milk production and some milk quality characteristics in lactating cows under field conditions.

• Journal of The Korean Society of Integrative Medicine
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• v.11 no.4
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• pp.61-71
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• 2023

Purpose : Energy bars are increasingly popular among active individuals, yet their timing and nutrient combinations' impact on exercise adaptation remains unclear. This study aims to address this knowledge gap by investigating whether the combination of pre- and post-workout supplementation can synergistically enhance fitness and alleviate fatigue in trained CrossFit participants. Investigate if combining pre- and post-workout supplements can enhance fitness and blood lactate management in trained CrossFit participants, potentially improving exercise performance for this group. Methods : In a randomized crossover study, 20 trained CrossFit individuals (11 males, 9 females) completed thrice-weekly 60-minute CrossFit sessions for 3 weeks, with a one-week washout period. Participants were randomly assigned to either a chocolate bar group (CH, 45 g, 225 kcal) or an energy bar group (ES, 48 g, 238 kcal, with protein, caffeine, taurine, and BCAAs). For one week, participants consumed two bars of their assigned supplement five minutes before and after workouts. After a washout period, they switched supplements. Blood lactate levels and a visual analog scale (VAS) were assessed before, immediately after, and 30 minutes post-workout. Fitness tests (hand grip, broad jump, sit-ups) were conducted at baseline and 30 minutes post-final workout. Data were analyzed using two-way repeated measures ANOVA (p<.05), 95 % confidence intervals, and magnitude inferences. Results : Hand grip strength (t=-5.60, p=.000), broad jump (t=-3.43, p=.003) and sit up (t=-3.94, p=.001) were significantly increased in the ES group. Compared to CH group, there was a significant time and group interactions for blood lactate level (F=5.51, p=.008) and VAS(F=31.67, p=.000) in the ES group. Conclusion : Pre- and post-workout energy bar supplementation may have a beneficial effect on blood lactate clearance and fitness in trained CrossFit individual. The combination of proprietary supplements taken may provide benefits for removing the blood lactate during high-intensity functional exercise.

• Journal of Animal Science and Technology
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• v.49 no.6
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• pp.729-736
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• 2007

The objective of this study was to investigate the anti-oxidative effects of pyruvate, taurine and melatonin on sperm characteristics(motility, membrane integrity) and lipid peroxidation(LPO) for in vitro storage of boar semen. Semen was treated with various antioxidants such as pyruvate(1mM), taurine(50mM) and melatonin(100nM) with or without 100uM H2O2. Antioxidant treatments were significantly increased the sperm motility when compare to control group in all incubation periods(P≤0.05). Hypoosmotic swelling test(HOST), membrane integrity was similar to the result of motility. In lipid peroxidation measurement by TBA reactions of spermatozoal plasma membrane, malondialdehyde(MDA) level in control and antioxidant treatments were lower than those of antioxidant plus H2O2 or H2O2 treatment for 3 to 6 h incubation period. Relationships of evaluation methods for sperm viability were investigated by motility, membrane integrity and lipid peroxidation. Among evaluation methods, LPO vs motility and membrane integrity vs LPO were negatively correlated(－0.23~－0.92 and －0.68~－0.85), but membrane integrity vs motility was positively correlated (0.53~0.94) in all treatments. These experiments indicate that supplementation of antioxidant to the semen extender can increase the sperm motility and membrane integrity and decrease the lipid peroxidation of spermatozoal plasma membrane. The HOST might be utilized to evaluate the sperm quality instead of lipid peroxidation or motility.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.6
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• pp.1075-1082
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• 2000

본 논문에서는 게껍질분말 급여에 의한 천연 타우린 강화우유의 생산 가능성을 평가하고자 25두의 젖소를 5군으로 나누고, 각기 대조사료, 1%(1%CS), 3%(3%CS) 및 5% 게껍질분말사료(5%CS), 그리고 formaldehyde로 처리되 3% 게껍질분말사료 (3%FCS)를 30일간 급여하였다. 게껍질분말 단백질의 아미노산조성은 phenylalanine이 가장 높았고, glutamate, aspartate, glycine, serine histidine, arginine의 순으로 나타났으며, 다른 동물조직의 단백질과는 달리 branched-chain 아미노산의 함량이 비교적 낮았다. 게껍질에 함유된 유리아미노산 중 타우린은 가장 고농도로 존재하였으며, 특히 집게다리껍질에 509$\mu$mol/100 g이 함유되어 등뚜껑(319$\mu$mol/100g) 및 게껍질분말(296 $\mu$mol/100 g)에 비해 월등히 그 함량이 높았다. 게껍질분말의 급여가 우유의 타우린농도에 미치는 영향을 평가한 결과 p<0.01 수준에서 유의성이 관찰되었다. 1%CS군의 원유에는 5.84$\pm$0.73 $\mu$mole/100mL의 타우린이 함유되어 대조구 (4.84$\pm$0.29 $\mu$mol/100mL)에 비해 20%정도 더 높았으며, 3%CS군의 원유에는 7.21$\pm$0.77$\mu$mole/100 mL의 타우린이 함유되어 대조구에 비해 49%정도 유의적으로 증가하였다.(p<0.01). 한편, 3%FCS군의 경우 원유의 타우린농도가 3%CS군에 비해 유？거으로 감소하였고, 따라서 게껍질의 formaldehyde 처리는 반추위내에서 타우린을 보호하는데 효과적이지 못한 것으로 나타났다. 본 논문은 게껍질 급여에 의해 우유의 타우린농도가 증가하였음을 밝히는 최초의 보고이며, 본 연구의 결과는 앞으로 기타 축산제품의 타우린함량을 증가시키는데 폭넓게 응용되어질 수 있을 것이다.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.2
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• pp.149-158
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• 2019

This study investigated the effects of replacing fish meal (FM) with a mixture of four protein sources (wheat gluten, soy protein concentrate, tankage meal, and poultry byproduct meal) in an extruded pellet (EP) diet for olive flounder Paralichthys olivaceus. Five experimental diets were formulated with alternative proteins replacing 0%, 20%, 30%, 40%, and 50% of FM. Taurine and betaine were added as attractants in the diets. Triplicate groups of fish (initial body weight: $196{\pm}2g$) were fed the diets to apparent satiation. Over the course of a 6-month feeding trial, there were no significant differences between the groups in growth performance, feed utilization, survival, or villus height. The dry matter and protein digestibility of FM50 diet were significantly lower than those of the control diet at water temperatures below $18.5^{\circ}C$ in months 4 and 6. This is a highly significant first report on FM replacement in an EP diet given to olive flounder over a 6-month-long feeding period. It shows that the proper mixture of protein sources can replace up to 50% of FM in olive flounder EP diets with taurine and betaine supplementation. It also shows that 40% of FM could be safely replaced in EP diets during periods of low water temperature.

• Food Science and Preservation
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• v.30 no.1
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• pp.15-27
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• 2023

Jeju Sasa borealis (S. borealis) is indigenous to the Halla Mountain area of Jeju Island, Republic of Korea. However, its dominance has retarded the development of other plant species and lowered biodiversity in this region. The aim of this study was to determine whether S. borealis silage (SS) supplementation affects the chemical composition and antioxidant activity in the fresh meat of Korean native goats (Capra hircus coreanae). The experiment was conducted on 12 Korean native goats at the finisher stage. The feeding groups were the Control (total mixed ration, TMR) and the Treatment (80% TMR + 20% SS). The animals were adapted for two weeks and then subjected to a six-month breeding experiment. Meat samples were excised from the neck, loin, rib, front leg, and hind leg of the slaughtered animals. The meat derived from the treatment group contained more taurine and anserine than that derived from the control group. Both groups did not significantly differ in terms of ω-6/ω-3 fatty acid ratio. The loin and front leg of the treatment group contained significantly higher vitamin E levels than those of the control group. DPPH, ABTS, and FRAP analyses disclosed that the loin and front leg had significantly higher antioxidant activity (p<0.05) than the other parts. Moreover, the loin and front leg cuts of the treatment group had higher antioxidant activity than those of the control group. The present study demonstrated that S. borealis supplementation could effectively improve Korean native goat meat quality.