• 한국육종학회지
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• 제49권3호
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• pp.170-179
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• 2017

분자농업은 식물을 일종의 공장개념으로 확대 적용하여 산업적으로 가치가 높은 유용물질들을 대량생산하는 분야로 최근 많은 각광을 받고 있다. 그 중에서 벼 캘러스를 이용한 단백질 발현 시스템은 대량 배양이 가능하고 목적 단백질의 높은 발현율로 인한 산업화가 가능한 기술이다. 본 연구는 이러한 벼(Oryza sativa L.) 캘러스의 활용도를 높이기 위한 효율적인 형질전환 시스템을 구축하기 위해 수행되었다. 형질전환에 이용된 종자의 종피 제거 시 손을 이용함으로써 캘러스 유도율을 높여주었고, 6년 정도의 오래된 종자에서도 원활한 캘러스 유도가 가능하였다. 목적 유전자가 도입된 캘러스의 선발은 최소한 3주 이상의 배양기간을 필요로 하였고 가장 효율적인 것은 한번의 계대를 포함한 6주 배양 후 선발하는 것이었다. 이러한 선발은 유전자가 식물세포의 genomic DNA 안에 안정적으로 삽입되어 이루어졌음을 서던 블롯 분석 및 후대검정 등을 통하여 확인할 수 있었다. 이러한 장기적이고 대량의 배양이 가능한 벼 캘러스의 효율적인 선발 시스템은 벼를 이용한 유용 재조합 단백질의 산업화에 실속있는 기술로 기여할 수 있을 것이다.

• 수산해양기술연구
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• 제34권3호
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• pp.259-273
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• 1998

The hydroacoustic surveys to provide the essential information for the assessment, management and utilization of fishery resources in the southern waters of Korea were carried out during five research cruises between October 1996 and October 1997 by the training ship KAYA of Pukyong National University. These hydroacoustic investigations were designed to obtain more precise estimates of the geographic distribution, absolute abundance and biological characteristics of the fishery resources, and the vertically integrated densities of fish in terms of volume backscattering strength(SV) by survey region and depth bins, such as the entire water column and the 0~ 10 m from bottom fraction, were measured separately. Hydroacoustic data were collected by using a Simrad EK 500 Scientific echo sounder operating at two frequencies of 38kHz and 120kHz and the data stored in field were later processed on a HP PC using a Simrad EP 500 echo integration and target strength analysis system. The biological compositions of echo signal were identified and sampled using a demersal trawl during daylight hours. The mean target strength to scale the echo integration data for hydroacoustic surveys was derived from the relationship between the SV and the weight of trawl catch per unit volume of the water column sampled by demersal trawls. The results obtained can be summarized as follow : 1. The mean volume backscattering strength for the entire water column in the southern waters of Korea between 1996 and 1997 were -67.2 dB and -70.9 dB at two frequencies of 38 kHz and 120 kHz , respectively, and for the bottom layer of the 0-10 m from bottom friction were -68.8 dB, -70.2 dB, respectively. That is, the volume backscattering strength for the entire water column at low frequency was higher than that at high frequency. 2. The relationship between the mean backscattering strength (〈SV〉, dB) for the depth strata of trawl hauls and the weight (C, kg/m3) per cubic meter of the catch sampled by bottom trawling in the southern waters of Korea in January and July 1997 were expressed by the following equations: 38 kHz : 〈SV〉= -28.2 + 10 log(C), 120 kHz : 〈SV〉= -32.4 + 10 log(C). The mean weight -normalized target strengths derived from these equitions were -28.2 dB/ kg, -32.4 dB/ kg at 38 kHz and 120 kHz , respectively. That is, the mean weight -normalized target strength at 38 kHz was 4.2 dB higher than that at 120 kHz. 3. The distribution density of fish in terms of biomass per unit volume in the southern waters of Korea were estimated to be 125.9 $\times$ 10-6 kg/m3 and 141.3 $\times$ 10-6 kg/m3 at 38 kHz and 120 kHz , respectively.

• Journal of Biosystems Engineering
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• 제37권1호
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• pp.19-27
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• 2012

Purpose: Utilizing air thermal energy during over-heated time in the greenhouse is a necessary component to save greenhouse heating costs for nighttime. However, there is no practical way to implement the related principles. Methods: In this study, a heating and cooling system which utilizes the surplus air thermal energy in a greenhouse was developed. Available air thermal energy and heating load for this experimental glasshouse were estimated based on temperature conditions of the plant growth and weather data. Results: Estimated values were 400 MJ/day for maximum surplus air thermal energy and 340 MJ/day for maximum heating energy which were target values of the design as well. The system consists of a heat pump, fan-coil units and heat storage tanks which are divided into low and high temperature tanks. Moreover, a new control logic was developed for surplus air thermal energy utilization. Conclusions: This paper explains the details of conceptual design process of the system. Results of test operations showed that the developed system performed the recovery and supply of the thermal energy according to design purposes.

• 한국의료질향상학회지
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• 제8권2호
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• pp.186-207
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• 2001

Background : This study presented an analysis of healthcare quality indicators using data mining and a development of decision support system for quality improvement. Method : Specifically, important factors influencing the key quality indicators were identified using a decision tree method for data mining based on 8,405 patients who discharged from a medical center during the period between December 1, 2000 and January 31, 2001. In addition, a decision support system was developed to analyze and monitor trends of these quality indicators using a Visual Basic 6.0. Guidelines and tutorial for quality improvement activities were also included in the system. Result : Among 12 selected quality indicators, decision tree analysis was performed for 3 indicators ; unscheduled readmission due to the same or related condition, unscheduled return to intensive care unit, and inpatient mortality which have a volume bigger than 100 cases during the period. The optimum range of target group in healthcare quality indicators were identified from the gain chart. Important influencing factors for these 3 indicators were: diagnosis, attribute of the disease, and age of the patient in unscheduled returns to ICU group ; and length of stay, diagnosis, and belonging department in inpatient mortality group. Conclusion : We developed a decision support system through analysis of healthcare quality indicators and data mining technique which can be effectively implemented for utilization review and quality management in a healthcare organization. In the future, further number of quality indicators should be developed to effectively support a hospital-wide Continuous Quality Improvement activity. Through these endevours, a decision support system can be developed and the newly developed decision support system should be well integrated with the hospital Order Communication System to support concurrent review, utilization review, quality and risk management.

• 한국환경복원기술학회지
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• 제20권6호
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• pp.21-33
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• 2017

The purpose of this study is to find out that it can be provided as a basic data to increase the satisfaction with users in the placement and creation of the Green Buffer Zone. In this study, the research was started on the Green Buffer Zone in Gwanggyo new town, which was rebuilt as the zone to use according to the change of legal installation standard of the zone. The subject of this study is the Green Buffer Zone in Gwanggyo new town, which is expected to be completed in step 4 of 2016. As a result of analysing the location and use characteristics of the Green Buffer Zone, the following conclusions were made. First of all, it is categorized into two categories by Buffering / disaster prevention type and land to use adjustment type. Second, the buffer space of commercial space and housing is satisfying, but in commercial space, there is a need for a cushioning function and housing spaces require improvements in the functioning of the landscape. Third, the function of the Green Buffer Zone resets to cushioning function, disaster prevention function, landscape function, utilization function. and The installation criteria are proposed to environmental pollution, noise abatement, and planting trees, etc. Lastly, the future utilization plan considering the location and use characteristics of the Green Buffer Zone is as follows. In the planning stage, preference is given to the characteristics of use of arranging green facility. It is necessary to manage the planned facilities as much as possible by changing them if necessary. Construction stage, it restrictively reflects landscaping facilities within the Green Buffer Zone function. After stage, This study will prioritize the target areas and select necessary areas and need to make efforts to prevent indiscriminate greenery encroachment.

• 한국비블리아학회지
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• 제23권1호
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• pp.131-151
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• 2012

최근 공공기관은 물론 학술정보 서비스 기관에서는 보유하고 있는 정보자원을 외부 기관과 공유함으로써 자원의 재활용을 통해 새로운 가치를 창출할 수 있도록 하고 있다. 특히 학술정보 서비스 기관은 디지털 콘텐츠의 확산 정책을 명문화함으로써, 학술정보 서비스의 일관성과 신뢰성, 정체성과 효율성을 제고시킬 필요가 있다. 이 연구에서는 학술정보 서비스 기관의 디지털 콘텐츠 확산 정책 수립을 위하여 국내외 디지털 콘텐츠 서비스 정책 및 확산 정책의 사례를 파악한 후, 한국과학기술정보연구원의 국가과학기술정보센터(NDSL)를 중심으로 확산 정책을 수립하였다. 디지털 콘텐츠 확산 정책의 주요 내용으로 목적 및 목표, 주 이용자, 확산 범위, 확산 방식, 활용에 대한 신청, 동의 및 협약 등을 제시하였다. 이 연구는 향후 유관 학술정보 서비스 기관의 디지털 콘텐츠 공유 및 확산을 위한 정책 도출에 기반이 될 것이다.

• 한국지반공학회:학술대회논문집
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• 한국지반공학회 2006년도 춘계 학술발표회 논문집
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• pp.644-651
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• 2006

In general, a damage that occurs due to landslide or slope failure in urban areas is larger than that in rural areas. In order to reduce the damage, a program is necessary to categorize slopes based on properties and to manage them systematically. Based on the above necessity, a slope management program which is applicable to slope management in an urban area has been developed at Hwangryung mountain in Busan as a target area. The program has a function of systematic slope information constructed by slope ID number of each slope or sub-region of a mountain, making a slope data sheet, analysis and grouping of slope stability, and establishment of a data base. It can also be utilized practically by end users due to the convenient input, edition, printing, management and operation of slope data. For practical utilization of the developed program, a research related to construction of the slope management system for a regional area is demanded to be performed continuously. The supply and utilization of a web based slope management system would contribute to damage reduction.

• 한국축산식품학회지
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• 제31권4호
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• pp.506-512
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• 2011

This study was conducted to validate the theoretical feasibility of a technique to identify biomarkers in Korean native black pig (KNP) and a commercial Landrace breed. Using two-dimensional electrophoresis, we found six proteins (NADH dehydrogenase Fe-S protein 1, an unnamed protein product, similar to T-complex protein 1, annexin V = CaBP33 isoform, fatty acid-binding protein, and catechol O-methyltransferase), which appeared in KNP alone. We raised polyclonal antibodies (used as the primary antibody) for Western blotting to confirm the characteristics of the six KNP proteins. As a result, catechol O-methyltransferase, annexin V = CaBP33 isoform, and the unnamed protein product presented thicker bands in KNP than those in Landrace. Moreover, catechol O-methyltransferase was shown to be more feasible as a biomarker for KNP. However, cross-reactivity was observed with the polyclonal antibodies for KNP and the other three proteins (NADH dehydrogenase, a protein similar to T-complex protein 1, and fatty acid-binding protein). This study only showed limited results from a limited number of animals; however, our research suggests possibilities for future studies.

• 대한설비공학회:학술대회논문집
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• 대한설비공학회 2009년도 하계학술발표대회 논문집
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• pp.1470-1475
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• 2009

Until now for the safety of structures and equipment monitoring technology to measure the amount of the physical, if that is the one, one-point or single-source target is one the most. Therefore, becoming more numerous and complex to measure the amount of physical measurement technology that is comprehensive and complex, multi-source concepts to the monitoring of a multi-sensing technology is required. Have the same characteristics of multi-source multi-use space such as a multi-structure of facilities/equipment is. The people's safety in a multi-use facility will be directly related to life and even a little carelessness can lead to large-scale disaster occurs because of several factors, risks and to manage detect in advance the development of an intelligent monitoring technology is essential. Therefore, this study shows that multiple structures/facilities to improve the quality of human life in research to maintain a safe and comfortable living space for multi-source intelligence to the development of monitoring technology to achieve that goal, and the ubiquitous sensor network system on the basis of the wireless transmission module, and multiple research facilities/equipment for the ultra-small sensors for health monitoring study was performed.

• 한국주거학회논문집
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• 제27권5호
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• pp.55-63
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• 2016

This study intended to establish a direction for the revitalization of youth culture centers by examining the current status of youth centers operated in Daejeon and how they have been used. Four youth centers in Daejeon were visited and a survey was conducted targeting 180 people using the centers. Frequency, Mean, and ${\chi}^2$ analysis were performed by using SPSS statistics package, and major research outcomes are as follows; There were 6 youth culture centers in Daejeon and showed a lower construction rate of 7.4% on the basis of 81 up, myun and dong. Most youth centers were small, around $300-500m^2$ in total floor area, and accommodated 100-200 people, and the space comprised multipurpose hall, cafeteria, open space, club space, multimedia space, information service room, and guide booth. There were no sports spaces among the target facilities. So, it has created the need for physical activity space to promote health & development. Most users visited centers with their friends and simply to use the facilities, and the satisfaction with the facilities was relatively high at 4.32. The role of the local community and financial support of the government is required to activate the youth culture. Also, for the role of youth culture center, it was suggested that the youth culture center should develop program which corresponds with the level of the youth and boast the interest of the youth.