• Journal of the Korean Society for Aviation and Aeronautics
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• v.23 no.4
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• pp.117-124
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• 2015

RAM (Reliability, Availability and Maintenance) has been applied to design and analysis tools which affects system's operational sustainability and its life cycle cost as RAM-c(2009 DoD). RAM-c plays also an important role to guarantee the system engineering for mission assurance. Reliability is highly related to the probability of system failure. Availability means mission capability or the condition of ready to mission. Maintenance includes both the repair to recover the system in the event of failure/unexpected breakdown and proactive maintenance to prolong the design service life of the system or machinery. It is the purpose that this paper is to analyze and conclude the objective service life of UAV. The more UAV is operated, the less the level of its reliability becomes. Repairing failures and supplying spare parts on time, system reliability could be improved up until the time over target. Applying statistical Weibull distributions, this paper suggests the analysis of the design service life and economic life of UAV based on RAM-c with operational data.

• BMB Reports
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• v.36 no.3
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• pp.299-304
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• 2003

For deciphering the cyclic guanosine monophosphate (cGMP) signaling pathway, we employed chemical proteomics to identify the novel target molecules of cGMP. We used cGMP that was immobilized onto agarose beads with linkers directed at three different positions of cGMP. We performed a pull-down assay using the beads as baits on tissue lysates and identified 9 proteins by MALDI-TOF (Matrix-Assisted Laser Desorption/Ionization Time-of-Flight) mass spectrometry. Some of the identified proteins were previously known cGMP targets, including cGMP-dependent protein kinase and cGMP-stimulated phosphodiesterase. Surprisingly, some of the co-precipitated proteins were never formerly reported to associate with the cGMP signaling pathway. The competition binding assays showed that the interactions are not by nonspecific binding to either the linker or bead itself, but by specific binding to cGMP. Furthermore, we observed that the interactions are highly specific to cGMP against other nucleotides, such as cyclic adenosine monophosphate (cAMP) and 5'-GMP, which are structurally similar to cGMP. As one of the identified targets, MAPK1 was confirmed by immunoblotting with an anti-MAPK1 antibody. For further proof, we observed that the membrane-permeable cGMP (8-bromo cyclic GMP) stimulated mitogen-activated protein kinase 1 signaling in the treated cells. Our present study suggests that chemical proteomics can be a very useful and powerful technique for identifying the target proteins of small bioactive molecules.

• Bulletin of the Korean Chemical Society
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• v.28 no.6
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• pp.947-952
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• 2007

Mycobacterium tuberculosis is a pathogen responsible for 2-3 million deaths every year worldwide. The emergence of drug-resistant and multidrug-resistant tuberculosis has increased the need to identify new antituberculosis targets. Acetohydroxy acid synthase, (AHAS, EC 2.2.1.6), an enzyme involved in branched-chain amino acid synthesis, has recently been identified as a potential anti-tuberculosis target. To assist in the search for new inhibitors and “receptor-based” design of effective inhibitors of tubercular AHAS (TbAHAS), we constructed four different structural models of TbAHAS and used one of the models as a target for virtual screening of potential inhibitors. The quality of each model was assessed stereochemically by PROCHECK and found to be reliable. Up to 89% of the amino acid residues in the structural models were located in the most favored regions of the Ramachandran plot, which indicates that the conformation of each residue in the models is good. In the models, residues at the herbicide-binding site were highly conserved across 39 AHAS sequences. The binding mode of TbAHAS with a sulfonylurea herbicide was characterized by 32 hydrophobic interactions, the majority of which were contributed by residue Trp516. The model based on the highest resolution X-ray structure of yeast AHAS was used as the target for virtual screening of a chemical database containing 8300 molecules with a heterocyclic ring. We developed a short list of molecules that were predicted to bind with high scores to TbAHAS in a conformation similar to that of sulfonylurea derivatives. Five sulfonylurea herbicides that were calculated to efficiently bind TbAHAS were experimentally verified and found to inhibit enzyme activity at micromolar concentrations. The data suggest that this time-saving and costeffective computational approach can be used to discover new TbAHAS inhibitors. The list of chemicals studied in this work is supplied to facilitate independent experimental verification of the computational approach.

• ALGAE
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• v.31 no.2
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• pp.167-174
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• 2016

Biological response of cells to variable conditions should affect the expression level of certain genes. Quantification of these changes in target genes needs stable internal controls. Real-time quantitative polymerase chain reaction (PCR) has traditionally used reference or ‘housekeeping’ genes, that are considered to maintain equal expression in different conditions, to evaluate changes in target genes between samples and experimental conditions. Recent studies showed that some housekeeping genes may vary considerably in certain biological samples. This has not been evaluated in red algae. In order to identify the optimal internal controls for real-time PCR, we studied the expression of eleven commonly used housekeeping genes; elongation factor 1-alpha, glyceraldehyde-3-phosphate dehydrogenase, β-actin, polyubiquitin, 30S ribosomal gene, 60S ribosomal gene, beta-tubulin, alpha-tubulin, translation initiation factor, ubiquitin-conjugating enzyme, and isocitrate dehydrogenase in different life-history stages of Bostrychia moritziana. Our results suggest that glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and 30S ribosomal gene, have the most stable gene expression levels between the different life history stages (male, female, carposporophyte, and tetrasporophyte), while the other genes are not satisfactory as internal controls. These results suggest that the combinations of GAPDH and 30S would be useful as internal controls to assess expression level changes in genes that may control different physiological processes in this organism or that may change in different life history stages. These results may also be useful in other red algal systems.

• Applied Chemistry for Engineering
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• v.21 no.5
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• pp.559-563
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• 2010

The representation of color with natural dyes is closely related with the modern well-being life. This study is focused on the representation of target color of the blue, yellow, and red color system, these color sources were originated from the green tea, gardenia, and black rice respectively. The quantitative numerical value of brightness (L), color coordinates (a, b) were designated for the target color, the optimum color nearest to the target value was extracted with the solvent pH, temperature, and extraction time. The longer the extraction time had more thickening color in case of the gardenia. The L-a-b coordinate of extracted color were analyzed with the color difference meter and compared with the target color. The experimental variables were optimized to extract the color of the smallest ${\Delta}E$ with the target color. As a result, the CIE standard value was proposed and the color was represented.

• Journal of Microbiology and Biotechnology
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• v.16 no.11
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• pp.1778-1783
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• 2006

Event-specific PCR detection methods are the primary trend in genetically modified (GM) plant detection owing to their high specificity based on the flanking sequence of the exogenous integrant. Therefore, this study describes a real-time PCR system for event-specific GM canola GT73, consisting of a set of primers, TaqMan probe, and single target standard plasmid. For the specific detection of GT73 canola, the 3'-integration junction sequence between the host plant DNA and the integrated specific border was targeted. To validate the proposed method, test samples of 0, 1, 3, 5, and 10% GT73 canola were quantified. The method was also assayed with 15 different plants, and no amplification signal was observed in a real-time PCR assay with any of the species tested, other than GT73 canola.

• Journal of Families and Better Life
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• v.20 no.2
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• pp.29-42
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• 2002

In this paper, we investigate the various opportunities of women informatization, which we believe to be major contributors for the high quality of life for women. We first justify various efforts for women informatization, which needs to be based on the analysis of the problems and the status quo. We have also examined the related government policies and programs as well as the programs of non-profit organizations for woman, and identified key problems areas. Integrating the results from this analysis, we have developed the vision and the framework for women informatization. We also give a list of strategic alternatives for improving quality of life through informatization, and promoting full-time housewives to become aggressive information users, information producers and information specialists. We use the expert Delphi method and fish-bone chart analysis to generate the priorities of specific tasks and target-based strategies for successful implementation of women informatization. We conclude this paper with various recommendations for empowering women to enjoy high-quality living through the process of informatization.

• Proceedings of the KIEE Conference
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• 2003.10a
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• pp.19-21
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• 2003

In this paper, we studied on life-decision of underground cable of live-lines state. As all equipments have been wear, underground cables decided design-life on the whole 30 years because underground cable have been occurred aging as time goes. CV cable has been become about 30 years after installation in the South Korea, is come to a important point of time with estimation about life. Study target cable is 22 kV CV cables in this point of view and installation cable is about 10 years before and behind. Measurement method used dc leakage method of live-lines state that applied voltage of 50V in neutral point and data is analyzing result that is measured during 5 years. In this result, insulation resistance could confirm that change according to season and cause is effect of humidity, seasons and load current. Also, according as data is gone aging, insulation resistance by Weibull distribution could confirm functionally its decrease. As a result, the aging speed of cable that water tree is gone could confirm fastness very. Numerical analysis result, cable that water tree is not gone could confirm that life of cable that has passed 10 years remains about $10{\sim}20$ years.

• Journal of Families and Better Life
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• v.27 no.6
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• pp.155-169
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• 2009

Resident participation plays much of a rule to the cohousing, which is organized independently and has the characteristics of community life, especially in the initial state of cohousing process. However, it is hard to realize the resident participation in proper order due to lack of a personal time, individual effort and an insufficient knowledge related with cohousing. So, it is thought that the guidance of cohousing expert and coordinator is needed to enable the residents to perform in an active attitude. The development of educational program and workshop connected with resident participation is also accompanied. In this study, to make the positive and systematic participation of cohousing residents, we established the workshop program and performed workshop with our developed program, based on preceding researches and examples. We selected the object of workshop as five housewives in thirties and forties. Workshop was consisted of six process steps. (1) Introduction and question of workshop and cohousing (2) examining and arranging the opinion (3) Master plan of cohousing village (4) Planning of common living space (5) Planning of individual living space (6) Analysis of final results and evaluation of workshop. The workshop held six times and it took about five~six hours per each meeting. Workshop participants expressed their opinion actively and attended in a positive manner in order to the design of their desired village and cohousing. During workshop process, the players established the common target of their village by means of life card, target card game. Also, based on the common target, the layout of village was planned using a collage game and card arrangement game. By workshop activity, the members started to recognize the significance of participation on the drawing board and improve their technique of communication and decision-making. Furthermore, workshop process made it possible to approach the concrete forms of their cohousing village. Especially, in the closing phase, the participant satisfaction about workshop and cohousing is increased suddenly and expressed their intention to join the real workshop related with cohousing.

• Journal of Korean Society for Atmospheric Environment
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• v.23 no.E2
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• pp.39-46
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• 2007

In order to present the scientific information of organic emissions from consumer products available, the current study examined the emissions composition for three different types of consumer products being used in Korea in an environmental chamber: air freshener (AF), mosquito repellents (MSQR), and moth repellents (MTHR). Emission rates were evaluated by determining emission rates of the target compound from the selected products using an electropolished stainless steel (SS) test chamber $(40{\times}25{\times}50cm^3)$. A time-dependent empirical relationship developed in this study agreed well with the test results. As same with the emission concentrations, MSQR exhibited the highest emission rate for all target compounds except for limonene and naphthalene. MTHR ($9,200\;{\mu}g\;h^{-1}$) showed the highest emission rate of naphthalene followed by MSQR ($8,300\;{\mu}g\;h^{-1}$). Moreover, the concentrations in residential bedroom conditions for target compounds emitted from three types of consumer products were estimated. This estimation suggests that the uses of consumer products can elevate indoor levels of target compounds. In particular, any types of the consumer products may increase the indoor level of m, p-xylene.