• Interdisciplinary Bio Central
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• 제3권1호
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• pp.3.1-3.16
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• 2011

Investigation of the genetic functions in complex biological systems is a challenging step in recent year. Hence, several valuable and interesting research projects have been developed with novel ideas to find out the unknown functions of genes or proteins. To validate the applicability of their novel ideas, various approaches are built up. To date, the most promising and commonly used approach for discovering the target proteins from biological system using small molecule is well known a forward chemical genetics which is considered to be more convenient than the classical genetics. Although, the forward chemical genetics consists of the three basic components, the target identification is the most challenging step to chemical biology researchers. Hence, the diverse target identification methods have been developed and adopted to disclose the small molecule bound protein. Herein, in this review, we briefly described the first two parts chemical toolbox and screening, and then the target identifications in forward chemical genetics are thoroughly described along with the illustrative real example case study. In the tabular form, the different biological active small molecules which are the successful examples of target identifications are accounted in this research review.

• 한국전자파학회논문지
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• 제17권12호
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• pp.1249-1258
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• 2006

ISAR 자동 초점(autofocus) 기법이란 움직이는 표적에 대한 2차원 레이더 영상인 ISAR 영상을 얻을 때 표적에 의한 doppler-shift 때문에 발생하는 위상 오차를 예측, 제거하는 기법이다. 이러한 자동 초점 기법이 수행되지 않은 ISAR 영상은 위상 오차(phase ewer)에 의한 왜곡이 포함되기 때문에 초점이 맞지 않는 것처럼 흐린(blurred) 영상이 된다. 이러한 영상은 추후에 구분기(classifier)를 이용하여 표적 구분 시 올바른 구분을 보장할 수 없으므로 반드시 자동 초점 기법이 수행되어야 하며, 본 논문에서는 이를 위해 영상의 엔트로피(entropy)를 최소화시키는 알고리즘을 이용한다. ISAR 자동 초점 기법은 크게 range alignment 과정과 phase adjustment 과정으로 나뉘는데, range alignment 과정에서 샘플링 포인트(sampling point)의 수와 그에 따른 영상 품질과의 관계를 분석하고, phase adjustment 과정 을 수행하기 위한 알고리즘인 SSA(Stage-by-Stage Approaching)의 계산시간 단축을 위한 방법을 분석한다.

• Bulletin of the Korean Chemical Society
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• 제27권5호
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• pp.657-662
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• 2006

Identification of accessible sites in targeted RNAs is a major limitation to the effectiveness of antisense oligonucleotides. A class of antisense oligodeoxynucleotides, known as the “10-23” DNA enzyme or DNAzyme, which is a small catalytic DNA, has been shown to efficiently cleave target RNA at purine-pyrimidine junctions in vitro. We have designed a strategy to identify accessible cleavage sites in the target RNA, which is hepatitis C virus nonstructural gene 3 (HCV NS3) RNA that encodes viral helicase and protease, from a pool of random DNAzyme library. A pool of DNAzymes of 58 nucleotides-length that possess randomized annealing arms, catalytic core sequence, and fixed 5'/3'-end flanking sequences was designed and screened for their ability to cleave the target RNA. The screening procedure, which includes binding of DNAzyme pool to the target RNA under inactive condition, selection and amplification of active DNAzymes, incubation of the selected DNAzymes with the target RNA, and target site identification on sequencing gels, identified 16 potential cleavage sites in the target RNA. Corresponding DNAzymes were constructed for the selected target sites and were tested for RNA-cleavage in terms of kinetics and accessibility. These selected DNAzymes were effective in cleaving the target RNA in the presence of $Mg^{2+}$. This strategy can be applicable to identify accessible sites in any target RNA for antisense oligonucleotides-based gene inactivation methods.

• Genomics & Informatics
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• 제14권4호
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• pp.241-254
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• 2016

Environmental microbes like Bordetella petrii has been established as a causative agent for various infectious diseases in human. Again, development of drug resistance in B. petrii challenged to combat against the infection. Identification of potential drug target and proposing a novel lead compound against the pathogen has a great aid and value. In this study, bioinformatics tools and technology have been applied to suggest a potential drug target by screening the proteome information of B. petrii DSM 12804 (accession No. PRJNA28135) from genome database of National Centre for Biotechnology information. In this regards, the inhibitory effect of nine natural compounds like ajoene (Allium sativum), allicin (A. sativum), cinnamaldehyde (Cinnamomum cassia), curcumin (Curcuma longa), gallotannin (active component of green tea and red wine), isoorientin (Anthopterus wardii), isovitexin (A. wardii), neral (Melissa officinalis), and vitexin (A. wardii) have been acknowledged with anti-bacterial properties and hence tested against identified drug target of B. petrii by implicating computational approach. The in silico studies revealed the hypothesis that lpxD could be a potential drug target and with recommendation of a strong inhibitory effect of selected natural compounds against infection caused due to B. petrii, would be further validated through in vitro experiments.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2001년도 하계학술대회 논문집 D
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• pp.1958-1960
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• 2001

In this paper, we propose the design methodology of target tracking system using the identification of TS fuzzy model based on genetic algorithm(GA) and RLS algorithm. In general, the objective of target tracking is to estimate the future trajectory of the target based on the past position of the target obtained from the sensor. In the conventional and mathematical nonlinear filtering method such as extended Kalman filter(EKF), the performance of the system may be deteriorated in highly nonlinear situation. In this paper, to resolve these problems of nonlinear filtering technique, the error of EKF by nonlinearity is compensated by identifying TS fuzzy model. In the proposed method, after composing training datum from the parameters of EKF, by identifying the premise and consequent parameters and the rule numbers of TS fuzzy model using GA, and by tuning finely the consequent parameters of TS fuzzy model using recursive least square(RLS) algorithm, the error of EKF is compensated. Finally, the proposed method is applied to three dimensional tracking problem, and the simulation results shows that the tracking performance is improved by the proposed method.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 1999년도 추계종합학술대회 논문집
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• pp.384-387
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• 1999

In this paper, we have proposed a testing methodology for Speed-Independent asynchronous control circuits using the self-checking property where the circuit detects certain classes of faults during normal operation. To exploit self-checking properties of Speed-Independent circuits, the Proposed methodology generates tests from the specification of the target circuit which describes the behavior of the circuit. The generated tests are applied to a fault-free and a faulty circuit, and target faults can be detected by the comparison of the outputs of the both circuits. For the purpose of efficient comparison, reachability information of the both circuits in the form of BDD's is used and operations are conducted by BDD manipulations. The identification for undetectable faults in testing is also used to increase efficiency of the proposed methodology. The proposed identification uses only topological information of the target circuit and reachability information of the good circuit which was generated in the course of preprocess. Experimental results show that high fault coverage is obtained for synthesized Speed-Independent circuits and the use of the identification process decreases the number of tests and execution time.

• Journal of Microbiology and Biotechnology
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• 제34권3호
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• pp.681-688
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• 2024

The accurate and rapid detection of methicillin-resistance of Staphylococcus aureus (SA) holds significant clinical importance. However, the methicillin-resistance detection strategies commonly require complicated cell lysis and gene extraction. Herein, we devised a novel colorimetric approach for the sensitive and accurate identification of methicillin-resistance of SA by combining allosteric probe-based target recognition with self-primer elongation-based target recycling. The PBP2a aptamer in the allosteric probe successfully identified the target MRSA, leading to the initiation of self-primer elongation based-cascade signal amplification. The peroxidase-like hemin/G-quadruplex undergo an isothermal autonomous process that effectively catalyzes the oxidation of ABTS2- and produces a distinct blue color, enabling the visual identification of MRSA at low concentrations. The method offers a shorter duration for bacteria cultivation compared to traditional susceptibility testing methods, as well as simplified manual procedures for gene analysis. The overall amplification time for this test is 60 min, and it has a detection limit of 3 CFU/ml. In addition, the approach has exceptional selectivity and reproducibility, demonstrating commendable performance when tested with real samples. Due to its advantages, this colorimetric assay exhibits considerable potential for integration into a sensor kit, thereby offering a viable and convenient alternative for the prompt and on-site detection of MRSA in patients with skin and soft tissue infections.

• 유공압시스템학회논문집
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• 제7권4호
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• pp.23-31
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• 2010

As the analysis and design technologies for electro-magnetic actuation has advanced over the years, proportional solenoid valve is gaining acceptance in wide range of industrial and commercial applications because of its superior characteristics over the conventional AOV or MOV, such as improved performance, reduced maintenance costs. This research deals with the position controller design of two-stage flow control solenoid valve. Investigation of steady-state characteristics and dynamic model identification for pilot disc is performed. Least square method to minimize the error magnitude of frequency response between the closed-loop and target system is applied to the design of PI-controller gains. From the experiments of step and frequency response, it is concluded that the controller meets the performance specification of target system, which verifies the usefulness of controller design method for proportional solenoid valve.

• 한국정보전자통신기술학회논문지
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• 제7권4호
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• pp.159-167
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• 2014

Local device-to-device (D2D) communication between two smart mobile devices is becoming increasingly popular. The first key step in starting a D2D communication is to discover and identify the remote target device to establish a link. However, existing device discovery mechanisms either require users to explicitly identify the ID of the target device or rely on inaccurate beamforming technology. This paper presents two novel device identification algorithms using a variety of embedded sensors. The algorithms only require that users to point two devices towards each other. This paper describes the algorithms, analyzes their accuracy using analytical models, and verifies the results using simulations.

• 대한의용생체공학회:의공학회지
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• 제14권2호
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• pp.185-193
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• 1993

In this paper, the peak detection algorithm was developed for the purpose of screening of the target compounds. Algorithm is divided into searching the characteristic ion and peak detection. The heuristic knowledge about analytical chemistry was applied for the searching the characteristic ion. Peak detection was accomplished in comparison with the peak identification strings and pattern strings around the retention time. Pattern strings are composed with the number which generated by pattern identification function. The variables of pattern identification function are the codes which represent the difference of two adjacent abundances Some of the free steroids were selected to demonstrate the proposed algorithm.