• BMB Reports
• /
• 제48권7호
• /
• pp.369-370
• /
• 2015

Actin dynamics is critical for the formation and sustainment of the immunological synapse (IS) during T cell interaction with antigen-presenting cells (APC). Thus, many actin regulating proteins are involved in spatial and temporal actin remodeling at the IS. However, little is known whether or how actin stabilizing protein controls IS and the consequent T cell functions. TAGLN2 − an actin-binding protein predominantly expressed in T cells − displays a novel function to stabilize cortical F-actin, thereby augmenting F-actin contents at the IS, and acquiring leukocyte function-associated antigen-1 activation following T cell activation. TAGLN2 also competes with cofilin to protect F-actin in vitro and in vivo. During cytotoxic T cell interaction with cancer cells, the expression level of TAGLN2 at the IS correlates with the T cell adhesion to target cancer cells and production of lytic granules such as granzyme B and perforin, thus expressing cytotoxic T cell function. These findings identify a novel function for TAGLN2 as an actin stabilizing protein that is essential for stable immunological synapse formation, thereby regulating T cell immunity. [BMB Reports 2015; 48(7): 369-370]

• 한국환경독성학회:학술대회논문집
• /
• 한국환경독성학회 1996년도 제19회정기학술대회(The 19th Symposium of the Korean Society of Environmental Toxicology)
• /
• pp.64-64
• /
• 1996

Transgenic animal and cell line models which are recently developed in toxicology field combined with molecular biological technique, are powerful tools for studying of mutation in vivo and in vitro, respectively. The Big Blue mutagenesis assay system is one of the most widely used transgenic systems. Especially, for the study of direct acting mutagens, Big Blue cell line is very useful and powerful to evaluate mutagenicity because the mutation frequency and mutationspectrlun showed no distinct differences between cell line and animal. The Big Blue cell lines carry stably integrated copies of lambda shuttle vector containing lac I gene as a mutational target. These lambda shuttle vectors are useful for various mutagenesis related studies in eukaryotic system due to their ability to be exposed mutagen and then transfer a suitable target DNA sequence to it convenient organism for analysis. We tried to assess the mutagenic effect of 4-NQO with Big Blue cell line. After the treatment of 4-NQO, genomic DNA was isolated and lambda shuttle vector was packaged by in Vitro packaging and then these were plated on bacterial host in the presence of X-gal to screen mutation in the lac I. We determined MF as a ratio of blue plaques versus colorless plaques and now undergoing the mutation spectrum of 4-NQO in lac J gene sequence.

• Safety and Health at Work
• /
• 제3권2호
• /
• pp.104-109
• /
• 2012

Singapore, an island republic of over 5 million inhabitants, has 3.1 million workers. Most are employed in the service, finance and tourist/transport industry. Significant numbers work in manufacturing, construction and heavy industry. Following a series of construction and shipyard accidents with multiple deaths in 2004, the government announced its intention to reduce workplace fatalities from 4.9 to 2.5 per 100,000 by 2015. There was strong political will to achieve this target. The strategic approaches were to build workplace safety and health (WSH) capabilities; implement legislative changes with enforcement; promote benefits of WSH and recognize best practices, and enhance partnership with stakeholders. The anticipated outcomes were to reduce workplace fatality and injury rates; have WSH as an integral part of business; and establish a progressive and pervasive WSH culture. With these measures, the workplace fatality rate declined from 4.9/100,000 in 2004, to 2.2/100,000 in 2010. However, other confounding factors could also account for this decline, and have to be considered. The next target, announced by Singapore's Prime Minister in 2008, is to further reduce the workplace fatality rate to 1.8/100,000 by 2018, and to have "one of the best workplace safety records in the world".

• 한국환경과학회지
• /
• 제15권8호
• /
• pp.737-744
• /
• 2006

Present study was designed to characterize the concentrations of major roadside air pollutants in Daegu and to compare with those of Seoul and Busan. Evaluated were the exceedance frequence of mean concentrations of target compounds(CO, NO$_2$, O$_3$, PM$_{10}$, SO$_2$) and the relationship for time variation. Two air pollution monitoring stations(one roadside station and one residential station) in Daegu were selected for this study. In addition, one roadside monitoring station from each of Seoul and Busan was chosen for the comparison of Daegu monitoring stations. The data analyzed in the current study were collected from 1998 to 2000 by Daegu Regional Environmental Management Office. The roadside concentrations of NO2 and PM to and the exceedance frequency of ambient air standard levels in Daegu were higher than those of Seoul and Busan. Except 03, the roadside concentrations of all target compounds showed following three distinguished patterns; first, possibly due to increased traffic density, the concentrations increased from 0500 to 0900(LST), second, the concentrations decreased from 0900 to 1700(LST) possibly due to the increased wind velocity and decreased traffic density, and finally, increased traffic density, the concentrations increased again from 1700 to 2100(LST). An implication was that major air pollution sources shifted from residential area to road-area during rush hours.

• 한국항공운항학회지
• /
• 제11권2호
• /
• pp.59-77
• /
• 2003

Air Traffic Control Tower is one of the most important units in Airport operation. It provides services related to safe and efficient traffic flows that control aircraft on the ground maneuvering area and terminal airspace. Also It is responsible for managing of ground operators. The major objective of this study is to evaluates relative efficiency of ATC towers in Domestic airports using data envelopment analysis so that it helps the ATC authority to improve the tower efficiency, to decide the level of benchmarking target and to establish the best alternative. The results of this study are the following; First, as a result of analysis for the potential improvement, it has analysed that the common problems of each ATC tower are to increase its number of flight and to reduce its number of runway followed by airside area, the number of air traffic controller and the number of stand. Second, it has shown that the each tower in RKPC(8), RKPT(5), RKPK(l) and RKSS(l) are used as the reference set. Especially, the tower in RKPC analyzed as a relatively efficient unit is the most main target for the towers in RKTU, RKTH, RKPS and RKTY to do bench marking and to set up the strategy for improving relative efficiency of the tower. Third, tower is actually not able to control the input and output data in this study except the number of controller, so that ATC authority is recommended to improve inefficiency of the towers through handling the number of controller.

• 한국군사과학기술학회지
• /
• 제21권2호
• /
• pp.147-157
• /
• 2018

There are increasing demands to provide early warning against intruding drones and cope with potential threats. Commercial anti-drone systems are mostly based on simple target detection by radar reflections. In real scenario, however, it becomes essential to obtain drone radar signatures so that hostile targets are recognized in advance. We present experimental test results that micro-Doppler radar signature delivers partial information on multi-rotor platforms and exhibits limited performance in drone recognition and classification. Afterward, we attempt to generate high resolution profile of flying drone targets. To this purpose, wide bands radar signals are employed to carry out inverse synthetic aperture radar(ISAR) imaging against moving drones. Following theoretical analysis, experimental field tests are carried out to acquire real target signals. Our preliminary tests demonstrate that high resolution ISAR imaging provides effective measures to detect and classify multiple drone targets in air.

• 한국정보처리학회논문지
• /
• 제6권7호
• /
• pp.1970-1979
• /
• 1999

본 논문에서는 수동 AVI 기술을 이용하여 차량 번호 판과 운전자 얼굴을 동시에 인식하는 시스템에 대해 제안하고자 한다. 이를 위해 우선적으로 환경에 불편인 전처리과정 알고리즘의 제시와 목표영역이 되는 차량 번호판 영역과 운전자 얼굴 영역을 추출하는 방법에 대해 다루고자 한다. 이후 목표영역에서 문자 영역분리와 인식 파라미터 추출을 행하고 차량 번호판의 경우 원형 정합으로, 운전자 얼굴 영역의 경우 퍼지 관계 행렬을 생성하여 최종적인 인식을 수행하고자 한다. 본 논문에서 제안하는 시스템은 환경에 불변인 전처리과정의 수행과 기존의 AVI 시스템에서 차량 번호 판만을 인식했던 것을 운전자 얼굴 인식까지 행함으로써 기존 AVI 시스템의 적용성 확대를 기할 수 있었다.

• Animal cells and systems
• /
• 제7권1호
• /
• pp.1-9
• /
• 2003

Programmed cell death, or apoptosis, is one of the most studied areas of modern biology. Apoptosis is a genetically regulated process, which plays an essential role in the development and homeostasis of higher organisms. Mitochondria, known to play a central role in regulating cellular metabolism, was found to be critical for regulating apoptosis induced under both physiological and pathological conditions. Mitochondria are a major source of reactive oxygen species (ROS) but they can also serve as its target during the apoptosis process. Release of apoptogenic factors from mitochondria, the best known of which is cytochrome c, leads to assembly of a large apoptosis-inducing complex called the apoptosome. Cysteine pretenses (called caspases) are recruited to this complex and, following their activation by proteolytic cleavage, activate other caspases, which in turn target for specific cleavage a large number of cellular proteins. The redox regulation of apoptosis during and after cytochrome c release is an area of intense investigation. This review summarizes what is known about the biological role of ROS and its targets in apoptosis with an emphasis on its intricate connections to mitochondria and the basic components of cell death.

• 한국지하수토양환경학회지:지하수토양환경
• /
• 제25권2_spc호
• /
• pp.55-69
• /
• 2020

Permeability-analyzing methods commonly involve small-scale drilling, such as pumping or slug test, but it is difficult to identify overall distribution of permeability of the entire target sites due to high cost and time requirement. Spectral induced polarization (SIP) method is known to be capable of providing distributions of both the porosity and the pore size, the two major parameters determining permeability of the porous medium. The relationship between SIP variables and permeability has been studied to identify the hydrological characteristics of target sites. Kozeny-Carman formula has been improved through many experiments to better predict fluid permeability with electrical properties. In this work, the permeability prediction techniques based on SIP data were presented in accordance with the hydrogeological and electrical characteristics of a porous medium. Following the summary of the techniques, various models and related laboratory experiments were analyzed and examined. In addition, the field applicability of the prediction model was evaluated by field case analysis.

• 한국건축친환경설비학회 논문집
• /
• 제12권6호
• /
• pp.557-566
• /
• 2018

The ZEB certification system has come into effect since 2018 according to the ZEB road map. From 2020, the public buildings with the total floor area smaller than $3000m^2$ are the target of the certification, and it will be extended to the buildings with up to $5000m^2$ floor area in 2025. However, current mandatory regulations for public office buildings seem already to meet the ZEB certification system planned for 2025. In this work, two buildings belong to $3,000{\sim}5,000m^2$ in total floor area were selected to analyze the possibility of meeting the ZEB certification only by following current obligation regulations. Results showed that the test buildings satisfied the minimum building energy efficiency and energy self-sufficiency rates for the ZEB certification when the mandatory insulation and installation ratio of renewable energy are applied. This can be useful for revising the road map or extending the target buildings of the ZEB certification.