• KSCE Journal of Civil and Environmental Engineering Research
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• v.29 no.2D
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• pp.245-253
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• 2009

Road facility is the most fundamental infrastructure for traffic and various information for smooth traffic is included in road surfaces. Various information included in road surfaces should be often removed and reinstalled by partial damage and aging. In addition, the existing road surface information should be removed in order to mark new information as traffic information changes. The existing road surface removal method suing grinders and torches had problems such as dangerous working equipment and workers' direct exposure to cars. In addition, although water-jet system using super high-pressure water was used to remove road surface in order of improvement of traditional method, there are another problems such as limitations according to water-tanks and water frost on the road surfaces after work. Therefore, this study analyzes and suggests systems to develop prototype after analyzing dry ice blaster in order to improve the current road surface removal methods. In addition, the study analyzes the possibility of introduction of dry ice blaster through a test for proposing an automatized equipment for new road surface mark removal considering environment and work efficiency, and compare traditional method with introduced dry ice blaster for operating cost.

• Journal of the Korean Institute of Gas
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• v.27 no.3
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• pp.123-133
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• 2023

Commercial hydrogen fuel cell vehicles are charged by compressing gaseous hydrogen to high pressure and storing it in a storage tank in the vehicle. This process causes the temperature of the gas to rise, to ensure the safety to storage tanks, the temperature is limited. Therefore, a heat transfer model is needed to explain this temperature rise. The heat transfer model includes the convective heat transfer phenomenon, and accurate estimation is required. In this study, the convective heat transfer coefficient in the hydrogen fueling process was calculated and compared using various correlation equations considering physical phenomena. The hydrogen fueling process was classified into the fueling line from the dispenser to the tank inlet and the storage tank in the vehicle, and the convective heat transfer coefficients were estimated according to process parameters such as mass flow rate, diameter, temperature and pressure. As a result, in the case of the inside of the filling line, the convective heat transfer coefficient was about 1000 times larger than that of the inside of the storage tank, and in the case of the outside of the filling line, the convective heat transfer coefficient was about 3 times larger than that of the outside of the storage tank. Finally, as a result of a comprehensive analysis of convective heat transfer coefficients in each process, it was found that outside the storage tank was lowest in the entire hydrogen fueling process, thus dominated the heat transfer phenomenon.

• The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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• v.13 no.2
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• pp.140-146
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• 2008

Marine ecotoxicological standard method using fish larvae was established with the standard test species of Oryzias latipes(Japanese Medaka) and Paralichthys olivaceus(flounder) and with the 7 day $LC_{50}$ as endpoint. Test method referred to the USEPA(1994) with the replacement of test species found in the Korean water. Standard test species were selected in terms of the species supply and ecological importance in Korean waters. Japanese medaka can be reared with small tanks in the lab and has wide tolerance on salinity, and flounder eggs can be easily obtained from commercial fish hatcheries. General conditions for larval fish toxicity test are as follows. The possible salinity ranges for toxicity test were $0{\sim}35\;psu$ for medaka and >20 psu for flounder. Test type was designated as static non-renewal test if the dissolved oxygen in the test chamber does not fall below 4.0 mg/L. Ages of test species were selected as 7 days after hatched for medaka(about 5 mm TL) and 25 days for flounder(about 10 mm TL) because of the low natural mortality after these periods. Test can be accepted when the survival rates are over 80% in control. Also, species sensitivity on standard reference materials(copper, cadmium or zinc) must be provided with the toxicity test results.

• Journal of Aerospace System Engineering
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• v.18 no.3
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• pp.70-75
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• 2024

The insulation performance of a common bulkhead propellant tank for small launch vehicles with variations in insulation thickness was analyzed. The common bulkhead propellant tank composed of a single part allows for lightweight design, as it eliminates the need for tank connections. However, problems such as propellant loss and ignition delay due to heat transfer caused by temperature differences between oxidizer and fuel may arise. Therefore, it is essential to verify the insulation performance of the common bulkhead structure that separates the oxidizer tank and fuel tank. In this study, transient heat transfer analysis was conducted for propellant tanks with insulation thicknesses of (50, 55, 60, 65, and 70) mm to analyze the insulation performance using boil-off mass. Subsequently, the boil-off mass of the oxidizer generated during the first-stage flight time of the propellant tank was determined. The results confirmed that increasing the insulation thickness reduces the boil-off mass, thereby improving the insulation performance.

• Fisheries and Aquatic Sciences
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• v.27 no.3
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• pp.180-194
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• 2024

Mass mortalities of mud crab Scylla spp. larvae due to pathogenic Vibrio spp. outbreaks have frequently occurred in hatcheries. To overcome this problem, probiotics containing Bacillus subtilis bacteria are applied to inhibit pathogenic ones. We tested different doses of probiotic-containing B. subtilis (10⁸ CFU/g) on the Scylla tranquebarica larvae and investigated the microbiota population, including Vibrio. Water quality, larvae development, and crablet production were also monitored. The recently hatched larvae were grown in twelve conical fiber tanks filled with 200 L sterile seawater, with a salinity of 30 ppt at a stocking density of 80 ind/L. Four different doses of probiotics were applied in the larvae rearing, namely, A = 2.5 mg/L, B = 5 mg/L, C = 7.5 mg/L, and D = 0 mg/L, with three replicates. Next-generation sequencing analysis was used to obtain the abundance of microbes in the whole body of megalopa and the water media for larvae rearing after applying probiotics. Sixteen Raw Deoxyribonucleic Acid samples (eight from a whole body of megalopa extraction from four treatments of probiotics defined as A, B, C, D, and eight from water media extraction from four treatments of probiotic defined as E, F, G, H) were prepared. Then, they were sent to the Genetics Science Laboratory for NGS analysis. Ammonia, nitrite, total organic matter (TOM), larvae, and crablet production were monitored. Based on the Next-generation sequencing analysis data, the Vibrio spp. decreased significantly (p < 0.05) than control test (D) in megalopa-applied probiotics at the doses of 2.5 mg/L (A) and 7.5 mg/L (C) and in the water media for megalopa rearing treated with probiotics at the dosage of 5.0 mg/L (F). Ammonia in the zoea stage in B treatment and TOM in the zoea and megalopa stage in B and C treatments were decreased significantly (p < 0.05). It impacts the higher number of zoea survival in treatments B and C. Finally, it results in a significantly high crablet production in treatments B and C. Therefore, the dosage of 5 mg/L to 7.5 mg/L improves crablet S. tranquebarica production significantly.

• Fisheries and Aquatic Sciences
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• v.26 no.12
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• pp.726-737
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• 2023

Piperine, the main bioactive component of black pepper (Piper nigrum Linn.), has anti-inflammatory, antifungal, and antibacterial properties. This study evaluated the supplemental effects of piperine or black pepper on innate immunity, growth, feed utilization efficiency, and intestinal morphology in red seabream (Pagrus major). Six experimental diets were formulated, supplementing piperine at 0.0, 0.25, 0.5, 1.0, and 2.0 g/kg levels (Con, P25, P50, P100, and P200) or 1.0 g/kg black pepper (BP100). Juvenile fish (7.6 ± 0.1 g) were randomly stocked into 18 circular tanks (220 L), including 30 fish per tank. Each diet was randomly assigned to triplicate groups, and the feeding trial was conducted for 8 weeks. The results showed that final body weight, specific growth rate, weight gain, and feed utilization efficiency were significantly improved (p < 0.05) when piperine was supplemented into diets at 0.25-2.0 g/kg levels compared to the Con group. Compared to the Con diet, condition factor was significantly increased (p < 0.05) in fish fed with dietary piperine at 0.25-2.0 g/kg or BP100 diet. Serum myeloperoxidase activity was increased (p < 0.05) in P25 and P100 groups and antiprotease activity was increased (p < 0.05) in P100 group compared to the Con group. Significantly higher (p < 0.05) lysozyme activity was observed in P50, P100, P200 and BP100 groups, while total immunoglobulin level was increased in P50, P100 and BP100 groups than Con group. Superoxide dismutase activity was increased (p < 0.05) by dietary piperine at 0.25-2.0 g/kg levels and BP100 diet compared to Con diet. Plasma cholesterol was significantly lower (p < 0.05) in fish fed with piperine (0.5-2.0 g/kg) or BP100 compared to the Con diet. Compared to the Con diet significantly longer (p < 0.05) intestinal villi were observed in fish fed with piperine at 0.25-1.0 g/kg levels, and higher goblet cell count was observed in P25 and BP100 groups. Dietary inclusion of piperine would be a potent immunostimulant in fish diet and the optimum supplementation level would be 0.25-1.0 g/kg.

• Korean Journal of Ichthyology
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• v.26 no.3
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• pp.185-193
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• 2014

To find the influence of water temperature, tank color and illumination in feeding, growth and blind-side hypermelanosis of starry flounder, Platichthys stellatus, in the present study, we performed a series of temperature, background color and illumination intensity test for 180 days (From June to December). The test was done in duplicate at 100 fish/tank (430% of initial covering area [PCA]) with the selected ordinary juvenile flounder (TL $17.3{\pm}0.5cm$, BW $82.5{\pm}0.2g$). The rearing was performed in darkgreen FRP aquarium tanks ($H100cm{\times}L100cm{\times}W100cm$; bottom area $1m^2$) lighten with average 1,000 lux and 230 lux, and in white FRP aquarium tank ($H100cm{\times}L100cm{\times}W100cm$; bottom area $1m^2$) lighten with average 230 lux of light intensity. We investigated correlation of daily food intake (DFI) with water temperature and salinity, and compared the influences of background colors and light intensity in DFI, food efficiency (FE), growth, survival rate, and ratio of malpigmented blind-side area and ambicolored fish ratio. In DFI, although it was not related with salinity, the amount was significantly decreased under 0.5 g/fish/day in summer and winter season, but was significantly increased over 1.5 g/fish/day in autumn season showing from $10^{\circ}C$ to $20^{\circ}C$ in water temperature. In background and illumination test, DFI, FE and survival rate showed no difference among three groups. The ratios of malpigmented blind-side area and ambicolored fish were also not significantly different among three groups, indicating that the blind-side hypermelanosis of starry may be governed not by background color (or light intensity) but by a genetics external trait inherited from parents.

• Korean Journal of Environmental Biology
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• v.27 no.3
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• pp.285-291
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• 2009

The effects of water temperature on oxygen consumption (OC) of starry flounder Platichthys stellatus reared in seawater (SW) and freshwater (FW) was performed in closed water-recirculating system containing respiratory chamber. Fish acclimated in separate indoor tanks with SW (nine of fish used, $263.0{\pm}40.4$ g) or FW (nine of fish used, $265.8{\pm}34.8$ g) were sampled. The OC of starry flounder at $15^{\circ}C,\;20^{\circ}C$ and $25^{\circ}C$ were $74.4{\pm}17.0,\;85.9{\pm}15.8,\;98.3{\pm}11.4\;mg\;O_2\;kg^{-1}hr^{-1}$ in SW and $46.7{\pm}12.0,\;63.3{\pm}7.5,\;82.6{\pm}5.3\;mg\;O_2\;kg^{-1}hr^{-1}$ in FW, respectively, showing a linear increase in OC with water temperature. The OC of fish reared in both SW and FW clear diel rhythm, with lower values at daytime and higher values in the night, in accordance with light (09:00~21:00 hr) and dark (21:00~09:00 hr) phases of the diel cycle (12L : 12D) in water temperature at $15^{\circ}C$ and $20^{\circ}C$. However OC of fish reared in both SW and FW showed unclear diel rhythm with light and dark phases of the diel cycle in water temperature at $25^{\circ}C$. Starry flounder reared in FW had higher ventilation rates than those in SW, but SW had higher OC per breath than those in FW.

• The korean journal of orthodontics
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• v.27 no.3 s.62
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• pp.503-513
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• 1997

This study was undertaken to investigate the effect of oxygen tension on the activity and function of the cells derived from human periodontal ligament by measuring cell activity, total protein synthesis, collagen synthesis, $IL-1{\beta},\;IL-6,\;TNF-{\alpha}$ Human periodontal ligament fibroblasts were collected from premolars extracted for orthodontic treatment and incubated in the environment of $37^{\circ}C,\;5\%\;CO_2,\100\%$ humidity. After the fifth to sixth passage they were used for the experiment. Gaspack system to which $0.2{\mu}m$ Millipore filter was attached was connected to mixed-gas tanks. The mixed gases were composed of $10\%\;O_2,\;5\%\;CO_2,\;85\%\;N_2$ in hyoxic group or $90\%\;O_2,\;5\%\;CO_2,\;5\%\;N_2$ in hyperoxic group and $5\%\;CO_2,\;95\%$ air for control. After incubation in $37^{\circ}C$ for 2, 4, 6 days, cell activity was determined by tetrazolium(MTT) assay and total protein synthesis was assayed using sulforhodamine B(SRB). And measurement of 4-hydroxyproline was performed to assess collagen synthesis md $IL-1{\beta},\;IL-6,\;and\;TNF-{\alpha}$ were measured by enzymeimmunoassay. The results were as follows. 1. The cell activity and total protein synthesis in hypoxic group were a little higher than or almost the same with those in control group. 2. In hyperoxic group, the cell activity was lower than that in control group and total protein synthesis was decreased. 3. Collagen synthesis was significantly decreased initially in both hypoxic and hyperoxic group and increased nearly to the level of control group as the duration of cell incubation was longer 4. As a result of enzymeimmunoassay, the amount of cytokines was $IL-6,\;TNF-{\alpha}\;and\;IL-1{\beta}$ in order. 5. $IL-6,\;TNF-{\alpha}\;and\;IL-1{\beta}$ were increased more rapidly in both hypoxic and hyperoxic group than in control group as the duration of cell incubation was longer. 6. There were more $IL-6\;and\;TNF-{\alpha}$ in hyperoxic group than in control group after 6 days, and there were more $IL-6\;and\;TNF-{\alpha}$ after 6 days than after 2 or 4 days in hyperoxic group. These results suggested that oxygen tension might modulate the production of extracellular matrix and cytokines in the cells derived from human periodontal ligament.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.26 no.2
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• pp.125-132
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• 1990

The authors carried out an experiment to investigate the responsive behavior of sea eel, Astroconger myriaster(BREVOORT) to chemical stimuli. The experimental tank was made in doughnut type by using FRP plates. The channel of tank was divided into three concentric troughs by using perforated plastic plates. The inside trough was used as influent part, the outside one as effluent part, and the middle one as the testing trough in which testing fish may be swimmable. The influent part was radially portioned into 12 sections so as to be 30 degrees of central angle. But a basin of any section in testing trough was diverged in the range of 45 degrees of central angle. The the testing trough are radially divided into eight zones. Water is supplied at the rate of 6.3l per minute from the central water tank set as high as 50cm in the center of doughnut, passed across the influent part, testing trough, effluent part and finally discharged by overflow pipes. The chemical substance to stimulate the sea eel was extracted from mackerel, shad and krill which are used as bait for fishing. The chemical substance was injected into any one of 12 hoses which supplies water from central water tank to the influent part at the rate of 2ml per minute. Sea eels used for the experiment were caught by pot in the coast of Chung-mu and accustomed to the tanks for 5 days before applying them to the experiment. The result obtained are as follows: 1. The rate of time length of sea eel's staying in the stimulated zone, as the extracts of mackerel, shad and krill were given to it, was observed as 7.9%, 30.9% and 11.4% respectively. It means that the extract from shad was the most effective of three in attracting sea eel. 2. To compare the effect of freshness of bait fish, the extracts were prepared from shad just killed, form the 24 hour-lapsed one and from the 48 hour-lasped one after killed. The rate of time length of sea eel's staying in the stimulated zone was 30.9%, 17.1% and 11.3% respectively. It means that the freshness is much effective in attracting olfactory fishes like sea eel.