• 아시안잔디학회지
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• 제22권1호
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• pp.13-24
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• 2008

한국잔디류의 교배조합 계통간 자웅성기관 발육의 차이를 구명하고, 형질의 유전특성을 파악하여, 자연 방임수분 조건에서 타가수분율을 추산하여, 자연방임조건의 교배를 이용한 한국잔디류의 합성품종의 육성 가능성을 확인해 보고자 본 연구를 수행하였다. 한국잔디류 중 형태적으로 특성이 있는 5개 종 및 품종을 사용하여 실험을 수행하였다. 교배조합을 포장에 식재한 후에 암술과 수술의 개화시기를 조사하였으며, 종자를 수확하여 육묘한 후에 후대들의 형태적 특성을 이용하여 형질의 유전특성을 조사하였다. 형태적 특성중 줄기의 기부색을 이용하여 타식률을 조사하였다. 자연방임수분 후대들의 형태적 특성을 이용한 유전특성 비교에서 잎털의 유무, 초장, 분얼경수는 변이의 폭이 크게 나타났고, 엽폭은 종자친의 형질을 많이 따랐으며, 전체적으로는 종자친과 화분친의 영향 없이 한쪽의 모본에서 영향을 받은 형이 가장 많이 나타났다. 모본간의 중간특성을 많이 나타낸 기부색은 각 개체들의 교배여부를 확인할 수 있는 지표로 활용할 수 있었다. 기부색을 이용한 타가수분율 추정은 자웅성기관의 개화시기가 비슷하고 양친의 특성에 차이가 있어 교배를 확인할 수 있는 조합에서 가능하였고, 교배 조합간에 타가수분율에 차이를 보였으나 본 실험에서는 타가수분율이 11.3-48.9(로 추산되어 타식이 발생되는 것을 확인하였고, 한국잔디가 방임수분을 통한 합성품종 육성의 가능성이 있는 것으로 판단되었다.

• 한국환경성돌연변이발암원학회지
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• 제24권1호
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• pp.33-39
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• 2004

To validate and to estimate the chemical hazard playa very important role to environment and human health. The detection of many synthetic chemicals including agrochemicals that may pose a genetic hazard in our environment is of great concern at present. Since these substances are not limited to the original products, and enter the environment, they have become widespread environmental pollutants, thus leading to a variety of chemicals that possibly threaten the public health. Pyrazosulfuron-ethyl [Ethyl-5-(4,6-dimethoxypyrimidin-2-ylcarbamoylsulfamoyl)-1-methylpyrazole-4-carboxylate, $C_{14}H_{18}N{6}O_{7}S,$ M.W. =414.39, CAS No. 93697-74-6], is one of well known rice herbicide belong in the sulfonyl urea group. To clarify the genotoxicity of this agrochemical, Ames bacterial reversion assay, in vitro chromosomal aberration assay with Chinese hamster lung (CHL) fibroblast and bone marrow micronucleus assay in mice were subjected. In Ames assay, although pyrazosulfuron-ethyl revealed cytotoxic at 5,000-140 $\mug/plate$ in Salmonella typhimurium TA100, no dose-dependent mutagenic potential in 4.4～70 $\mug/plate$ of S. typhimurium TA 98, TA 100, TA1535 and TA 1537 both in the absence and presence of S-9 metabolic activation system was observed. Using CHL fibroblasts, the 50% cell growth inhibition concentration $(IC_{50})$ of pyrazosulfuron-ethyl was determined as 1,243 $\mug/mL,$ and no chromosomal aberration was observed both in the absence and presence of S-9 mixture in the concentration range of 311-1,243 $\mug/mL.$ And also, in vivo micronucleus assay using mouse bone marrow, pyrazosulfuron-ethyl revealed no remarkable induction of MNPCE (micronucleated polychromatic erythrocytes/1000 polychromatic erythrocytes) in the dose range of 625-2,500 mg/kg body weight when administered orally. Consequently, Ames bacterial gene mutation with Salmonella typhimurium, in vitro chromosome aberration with mammalian cells and in vivo bone marrow micronucleus assay revealed no clastogenic potential of pyrazosulfuron-ethyl in this study.

• International Journal of Industrial Entomology and Biomaterials
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• 제5권2호
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• pp.141-151
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• 2002

The success of sericulture industry in India is mainly attributed to the well-planned annual sericultural activity and the systematic implementation of pest preventive and control measures. The insect spectrum of silkworm and its food plants is complex and plays a major role in limiting the production of silk. Insects cause extensive damage to plant whereas predators and parasites either kill the silkworm larvae or force them to spin flimsy cocoons. Unilateral control measure against this pest is mainly based on the use of synthetic organic insecticides. Though these approaches initially paid rich dividends, the undesirable consequences soon surfaced. Insecticide induced resurgence of gall midges, leafhopper, leaf roller, secondary pest out breaks and development of pest biotypes has led to realization of Integrated Pest Management in sericulture. Various components of IPM, viz. Host plant resistance, cultural practices, biological control, chemical control and integrating them at various technological levels have been studied. Sources of host plant resistance have been identified for some of the major insect pests. High yielding mulberry variety has been propagated and their resistances towards major pests have been recorded. Cultural practices like pruning, pollarding, judicious use of nitrogen, optimum spacing and weed management have preyed to be the powerful tools in containing pests. Natural control over the pest population build- up exerted by the wide range of parasitoids, predators and pathogens has been well documented with identification of natural enemies and studies on their potential. Augmentation, through inoculation or inundative releases of parasitic arthropods, is the most direct way of increasing the numbers of these beneficials in sericulture.

• 방송공학회논문지
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• 제19권5호
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• pp.656-664
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• 2014

안개 영상은 영상의 대비가 밝은 영역에 치우쳐 있기 때문에 영상의 정보를 전달하기 어렵다. 이러한 이유로 안개 제거 알고리즘이 연구되고 있다. 일반적으로 안개가 포함되기 전 상태의 영상을 획득하는 것이 어렵기 때문에 알고리즘의 성능을 평가하기 위해 결과 영상을 정성적으로 분석하였다. 본 논문에서는 영상의 변위 정보를 이용하여 안개 영상을 생성함으로써 정량적으로 오차를 비교하는 방법을 제안한다. 또한 이때 은닉 랜덤 마코프 모델(HRMF)에 기반한 기대값 최대화(EM) 알고리즘을 이용하여 블록 결함을 제거하였다. 다양한 합성영상 및 자연영상에 대하여 결과를 비교함으로써 제안한 알고리즘의 성능을 확인하였다.

• 한국지진공학회논문집
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• 제11권4호
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• pp.25-31
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• 2007

For areas such as the Korean Peninsula, which have moderate seismic activity but no available records of strong ground motion, synthetic seismograms can be used to evaluate ground motion without waiting for a strong earthquake. Such seismograms represent the estimated ground motions expected from a set of possible earthquake scenarios. Local site effects are especially important in assessing the seismic hazard and possible ground motion scenarios for a specific fault. The earthquake source and rupture dynamics can be described as a two-step process of rupture initiation and front propagation controlled by a frictional sliding mechanism. The seismic wavefield propagates through heterogeneous geological media and finally undergoes near-surface modulations such as amplification or deamplification. This is a complex system in which various scales of physical phenomena are integrated. A unified approach incorporates multi-scale problems of dynamic rupture, radiated wave propagation, and site effects into an all-in-one model using a three-dimensional, fourth-order, staggered-grid, finite-difference method. The method explains strong ground motions as products of complex systems that can be modified according to a variety of fine-scale rupture scenarios and friction models. A series of such deterministic earthquake scenarios can shed light on the kind of damage that would result and where it would be located.

• Asian Pacific Journal of Cancer Prevention
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• 제14권6호
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• pp.3631-3634
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• 2013

MicroRNAs (MiRNAs) play important roles in coordinating a variety of cellular processes and abnormal expression has been linked to the occurrence of several cancers. The miRNA miR-451 is downregulated in colorectal carcinoma (CRC) cells, suggested by several research groups including our own. In this study, synthetic miR-451 mimics were transfected into the SW620 human CRC cell line using Lipofectamine 2000 and expression of miR-451 was analyzed by real time PCR, while expression of CAB39, LKB1, AMPK, AKT, PI3K and Bcl2 was analyzed by Western blot, and cell growth was detected by MTT assay. In comparison to the controls, a significant increase in the expression of miR-451 was associated with significantly decreased expression of CAB39, LKB1, AMPK, AKT, PI3K and Bcl2. The capacity of cell proliferation was significantly decreased by miR-451 expression, which also inhibited cell growth. Our study confirmed that miR-451 has a repressive role in CRC cells by inhibiting cell growth through down-regulating the P13K/AKT pathway.

• 건축역사연구
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• 제12권3호
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• pp.117-130
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• 2003

Transparency orginated from a visual character of the object recognized to the backside without any obstacle is used as a word explaining the variety of an architectural character from the pre-mordern architecture. Transparency is expressed clearly without using of the material with a permeability like glass in the Korean traditional architecture. Thus the purpose of this study is to analyze an expression of the perceptive transparency focusing on the Dodong Seowon with the phenomenal transparency. In chapter one, the background, purpose and method of study are explained. In chapter two, general concept of transparency is inquired. In the process of searching for the formation of basic background, the characteristic of phenomenal transparency is recognized. In chapter three, the aspect of phenomenal tranparency expression in Korean traditional architecture is inquired. In chapter four, with the characteristics drawn from chapter three, the characteristics of Dodong-Seowon under its phenomenal tranparency are shown. In chapter five, synthetic conclusion is presented.

• 대한전자공학회:학술대회논문집
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• 대한전자공학회 2001년도 제14회 신호처리 합동 학술대회 논문집
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• pp.283-286
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• 2001

In this paper, we present a robust region segmentation method based on the watershed transformation of a pseudo-distance map (PDM). A usual approach for the segmentation of a gray-scale image with the watershed algorithm is to apply it to a gradient magnitude image or the Euclidean distance map (EDM) of an edge image. However, it is well known that this approach suffers from the oversegmentation of the given image due to noisy gradients or spurious edges caused by a thresholding operation. In this paper we show thor applying the watershed algorithm to the EDM, which is a regularized version of the EDM and is directly computed form the edgestrength function (ESF) of the input image, significantly reduces the oversegmentation, and the final segmentation results obtained by a simple region-merging process are more reliable and less noisy than those of the gradient-or EDM-based methods. We also propose a simple and efficient region-merging criterion considering both boundary strengths and inner intensities of regions to be merged. The robustness of our method is proven by testing it with a variety of synthetic and real images.

• BMB Reports
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• 제33권2호
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• pp.162-165
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• 2000

Polymerase chain reaction (PCR) is well established as an indispensable tool of molecular biology; and yet a limitation for cloning applications continues to be that products often require subsequent restriction to be that products often require subsequent restriction digests, blunt-end ligation, or the use of special linear vectors. Here a rapid, PCR-based system is described for the simple, restriction enzyme-free generation of synthetic, 'restriction-like' DNA fragments with staggered ends. Any 3'- or 5'-protruding terminus, but also non-palindromic overhangs with an unrestricted single strand length are specifically created. With longer overhangs, "Restriction-PCR" does not even require a ligation step prior to transformation. Thereby the technique presents a powerful tool e.g. for a successive, authentic reconstitution of sub-fragments of long genes with no need to manipulate the sequence or to introduce restriction sites. Since restriction enzyme-free and thereby devoid the limitations of partial DNA digests, "Restriction-PCR" allows a straight one-step generation and cloning of difficult DNA fragments that internally carry additional sites for specific sequence insertions or deletions can be precisely engineered into genes of interest. With these properties "Restriction-PCR" has the potential to add significant speed and versatility to a wide variety of DNA cloning applications.

• Mass Spectrometry Letters
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• 제9권3호
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• pp.81-85
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• 2018

In this study, the chemical composition of bacterial melanin isolated from the Streptomyces glaucescens strain was elucidated by ultra-high-resolution Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry. Ultra-high-resolution mass profiles of the microbial melanin product were acquired using a 15 Tesla FT-ICR mass spectrometer in positive and negative ion modes via electrospray ionization to obtain more complete descriptions of the molecular compositions of melanin-derived organic constituents. A mass resolving power of 500,000 (at m/z 400) was achieved for all spectra while collecting 400 scans per sample with a 4 M transient. The results of this analysis revealed that the melanin pigment isolated from S. glaucescens predominantly exhibits CHON and CHO species, which belong to the proteins class of compounds, with the mean C/O and C/N ratios of 4.3 and 13.1, thus suggesting that the melanin could be eumelanin. This analytical approach could be utilized to investigate the molecular compositions of a variety of natural or synthetic melanins. The compositional features of melanins are important for understanding their formation mechanisms and physico-chemical properties.