Asian Pacific Journal of Cancer Prevention

Asian Pacific Journal of Cancer Prevention (아시아태평양암예방학회)
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MicroRNA-451 Inhibits Growth of Human Colorectal Carcinoma Cells via Downregulation of Pi3k/Akt Pathway

  • Li, Hong-Yan (Department of Anus & Intestine Surgery, the Third Hospital of HeBei Medical University) ;
  • Zhang, Yan (Department of Anus & Intestine Surgery, the Third Hospital of HeBei Medical University) ;
  • Cai, Jian-Hui (Department of Surgery of Hebei Medical University) ;
  • Bian, Hong-Lei (Department of Anus & Intestine Surgery, the Third Hospital of HeBei Medical University)
  • Published : 2013.06.30
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Abstract

MicroRNAs (MiRNAs) play important roles in coordinating a variety of cellular processes and abnormal expression has been linked to the occurrence of several cancers. The miRNA miR-451 is downregulated in colorectal carcinoma (CRC) cells, suggested by several research groups including our own. In this study, synthetic miR-451 mimics were transfected into the SW620 human CRC cell line using Lipofectamine 2000 and expression of miR-451 was analyzed by real time PCR, while expression of CAB39, LKB1, AMPK, AKT, PI3K and Bcl2 was analyzed by Western blot, and cell growth was detected by MTT assay. In comparison to the controls, a significant increase in the expression of miR-451 was associated with significantly decreased expression of CAB39, LKB1, AMPK, AKT, PI3K and Bcl2. The capacity of cell proliferation was significantly decreased by miR-451 expression, which also inhibited cell growth. Our study confirmed that miR-451 has a repressive role in CRC cells by inhibiting cell growth through down-regulating the P13K/AKT pathway.

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References

  1. Akao Y, Nakagawa Y, Naoe T (2007). MicroRNA-143 and -145 in colon cancer. DNA Cell Biol, 26, 311-20. https://doi.org/10.1089/dna.2006.0550
  2. Bandres E, Bitarte N, Arias F, et al (2009). microRNA-451 regulates macrophage migration inhibitory factor production and proliferation of gastrointestinal cancer cells. Clin Cancer Res, 15, 2281-90. https://doi.org/10.1158/1078-0432.CCR-08-1818
  3. Bandres E, Cubedo E, Agirre X, et al (2006). Identification by Real-time PCR of 13 mature microRNAs differentially expressed in colorectal cancer and non-tumoral tissues. Mol Cancer, 5, 29. https://doi.org/10.1186/1476-4598-5-29
  4. Chen CZ, Li L, Lodish HF, Bartel DP (2004). MicroRNAs modulate hematopoietic lineage differentiation. Science, 303, 83-6. https://doi.org/10.1126/science.1091903
  5. Chen JY, Lin JR, Cimprich KA, Meyer T (2012). A twodimensional ERK-AKT signaling code for an NGF-triggered cell-fate decision. Mol Cell, 45, 196-209. https://doi.org/10.1016/j.molcel.2011.11.023
  6. Chen X, Guo X, Zhang H, et al (2009). Role of miR-143 targeting KRAS in colorectal tumorigenesis. Oncogene, 28, 1385-92. https://doi.org/10.1038/onc.2008.474
  7. Dong H, Tang J, Li LH, et al (2013). Serum carbohydrate antigen 19-9 as an indicator of liver metastasis in colorectal carcinoma cases. Asian Pac J Cancer Prev, 14, 909-13. https://doi.org/10.7314/APJCP.2013.14.2.909
  8. Eustace K (2005). Colorectal cancer. Lancet, 365, 166. https://doi.org/10.1016/S0140-6736(05)17707-1
  9. Gallia GL, Rand V, Siu IM, et al (2006). PIK3CA gene mutations in pediatric and adult glioblastoma multiforme. Mol Cancer Res, 4, 709-14. https://doi.org/10.1158/1541-7786.MCR-06-0172
  10. Gao W, Shen H, Liu L, et al (2011). MiR-21 overexpression in human primary squamous cell lung carcinoma is associated with poor patient prognosis. J Cancer Res Clin Oncol, 137, 557-66. https://doi.org/10.1007/s00432-010-0918-4
  11. Godlewski J, Nowicki MO, Bronisz A, et al (2010). MicroRNA-451 regulates LKB1/AMPK signaling and allows adaptation to metabolic stress in glioma cells. Mol Cell, 37, 620-32. https://doi.org/10.1016/j.molcel.2010.02.018
  12. Huang Q, Liu L, Liu CH, et al (2013). MicroRNA-21 Regulates the Invasion and Metastasis in Cholangiocarcinoma and May Be a Potential Biomarker for Cancer Prognosis. Asian Pac J Cancer Prev, 14, 829-34. https://doi.org/10.7314/APJCP.2013.14.2.829
  13. Hui AB, Shi W, Boutros PC, et al (2009). Robust global micro-RNA profiling with formalin-fixed paraffin-embedded breast cancer tissues. Lab Invest, 89, 597-606. https://doi.org/10.1038/labinvest.2009.12
  14. Jemal A, Bray F, Center MM, et al (2011). Global cancer statistics. CA Cancer J Clin, 61, 69-90. https://doi.org/10.3322/caac.20107
  15. Johnson R, Zuccato C, Belyaev ND, et al (2008). A microRNAbased gene dysregulation pathway in Huntington’s disease. Neurobiol Dis, 29, 438-45. https://doi.org/10.1016/j.nbd.2007.11.001
  16. Katada T, Ishiguro H, Kuwabara Y, et al (2009). microRNA expression profile in undifferentiated gastric cancer. Int J Oncol, 34, 537-42.
  17. Kita D, Yonekawa Y, Weller M, Ohgaki H (2007). PIK3CA alterations in primary (de novo) and secondary glioblastomas. Acta Neuropathol, 113, 295-302. https://doi.org/10.1007/s00401-006-0186-1
  18. Lee RC, Feinbaum RL, Ambros V (1993). The C. elegans heterochronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell, 75, 843-54. https://doi.org/10.1016/0092-8674(93)90529-Y
  19. Li X, Sanda T, Look AT, et al (2011). Repression of tumor suppressor miR-451 is essential for NOTCH1-induced oncogenesis in T-ALL. J Exp Med, 208, 663-75. https://doi.org/10.1084/jem.20102384
  20. Opel D, Westhoff MA, Bender A, et al (2008). Phosphatidylinositol 3-kinase inhibition broadly sensitizes glioblastoma cells to death receptor- and drug-induced apoptosis. Cancer Res, 68, 6271-80. https://doi.org/10.1158/0008-5472.CAN-07-6769
  21. Patrick DM, Zhang CC, Tao Y, et al (2010). Defective erythroid differentiation in miR-451 mutant mice mediated by 14-3-3zeta. Genes Dev, 24, 1614-9. https://doi.org/10.1101/gad.1942810
  22. Prevo R, Deutsch E, Sampson O, et al (2008). Class I PI3 kinase inhibition by the pyridinylfuranopyrimidine inhibitor PI-103 enhances tumor radiosensitivity. Cancer Res, 68, 5915-23. https://doi.org/10.1158/0008-5472.CAN-08-0757
  23. Ren H, Li J, Liu JJ, et al (2012). Anti-HER-2 anti-CD3 bispecific antibodies inhibit growth of HCT-116 colorectal carcinoma cells in vitro and in vivo. Asian Pac J Cancer Prev, 13, 2795-8. https://doi.org/10.7314/APJCP.2012.13.6.2795
  24. Song G, Ouyang G, Bao S (2005). The activation of Akt/PKB signaling pathway and cell survival. J Cell Mol Med, 9, 59-71. https://doi.org/10.1111/j.1582-4934.2005.tb00337.x
  25. Tian Y, Nan Y, H an L, et al (2012). MicroRNA miR-451 downregulates the PI3K/AKT pathway through CAB39 in human glioma. Int J Oncol, 40, 1105-12.
  26. Tong AW, Nemunaitis J (2008). Modulation of miRNA activity in human cancer: a new paradigm for cancer gene therapy? Cancer Gene Ther, 15,341-55. https://doi.org/10.1038/cgt.2008.8
  27. Wang T, Zang WQ, Li M, et al (2013). Effect of miR-451 on the Biological Behavior of the Esophageal Carcinoma Cell Line EC9706. Dig Dis Sci, 58, 706-14. https://doi.org/10.1007/s10620-012-2395-x
  28. Yang ZZ, Tschopp O, Baudry A, et al (2004). Physiological functions of protein kinase B/Akt. Biochem Soc Trans, 32, 350-4. https://doi.org/10.1042/BST0320350

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