• BMB Reports
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• 제43권11호
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• pp.732-737
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• 2010

RNA interference is a post-transcriptional silencing mechanism triggered by the bioavailability and/or exogenous introduction of double-stranded RNA (dsRNA) into cells. Here we describe a novel method for the synthesis of siRNA in a single vessel. The method employs in vitro transcription and a single-stranded DNA (ssDNA) template and design, which incorporates upon self-annealing, two promoters, two templates, and three loop regions. Using this method of synthesis we generated efficacious siRNAs designed to silence both exogenous and endogenous genes in mammalian cells. Due to its unique design the single-stranded template is easily amenable to adaptation for attachment to surface platforms for synthesis of siRNAs. A siRNA synthesis platform was generated using a 3' end-biotinylated ssDNA template tethered to a streptavidin coated surface that generates stable siRNAs under multiple cycles of production. Together these data demonstrate a unique and robust method for scalable siRNA synthesis with potential application in RNAi-based array systems.

• 한국정보통신학회논문지
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• 제16권3호
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• pp.571-578
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• 2012

본 논문에서는 크게 두 가지 사항에 대해서 제안하고자 한다. 첫 번째는 논리합성을 위한 제약조건 방법에 대한 것이고, 두 번째는 효율적인 논리합성방법에 대한 것이다. 논리 합성은 주어진 제약조건(constraint)을 최대한 만족 시키면서 논리 사상과 최적화 등을 통하여 RTL(register transfer level) 코드로부터 게이트-수준의 네트리스트를 얻는 과정이다. 논리합성의 결과는 주어진 제약조건과 합성 방법에 매우 종속적이다. 이들에 의해서 설계의 면적 및 타이밍이 크게 변화하므로 우리는 제약조건과 합성방법을 철저하게 고려하여야 한다. 본 논문에서는 논리합성을 하는 과정에서 실제로 고려해야하는 사항들에 대해서 경험적이고 실험적인 결과를 바탕으로 혼합방식의 논리합성 기법을 제안한다. 제안된 기법을 이용하여 약 65만 게이트의 하드웨어 자원량을 사용하는 회로에 적용시켜본 결과로 상향식 방법에 비해서 합성 시간이 약 47% 감소하였고, 하향식 방법에 비해서 타이밍 특성이 우수하였다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2004년도 추계학술대회 논문집
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• pp.1270-1273
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• 2004

The mechanism design is synthesis of suitable mechanism which can be output motions about input motions. That has generally two steps which are the type synthesis and the dimensional synthesis. And required mechanism analysis step for confirming middle or final result. The type synthesis is definition of mechanism type which required aim and the dimensional synthesis is calculation of dimension about defined type mechanism. The type synthesis of mechanism is included qualitative design field which isn't defined systematic design method. especially, the most difficult step for mechanism design automation. In this paper proposed the component modular design method which is figured mechanism types automate with component modules using component modular approach. And develop CAD(Computer Aided Program) program for application.

• Bulletin of the Korean Chemical Society
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• 제34권4호
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• pp.1070-1076
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• 2013

Herein is reported a simple and efficient synthesis of isoflavones starting from various substituted phenacyl bromides and salicylaldehydes in presence of NHC. The mechanism involved domino catalysis type approach with consumption and regeneration of catalyst in two catalytic cycles. This method proved to be very lucrative and gives very good yield. The method described here represents an environmentally benign alternative to classical approach.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2008년도 추계학술대회A
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• pp.822-826
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• 2008

A linkage mechanism is a device to convert an input motion into a desired output motion. Traditional linkage mechanism designs are based on trial and error approaches so that size or shape changes of an original mechanism often result in improper results. In order to resolve these problems, an improved automatic mechanism synthesis method that determines the linkage type and dimensions by using an optimization method during the synthesis process has been proposed. For the synthesis, a planar linkage is modeled as a set of rigid blocks connected by zero-length translational springs with variable stiffness. In this study, the sizes of rigid blocks were also treated as design variables for more general linkage synthesis. The values of spring stiffness and the size of rigid block yielding a desired output motion at the end-effecter are found by using an optimization method.

• 한국음향학회:학술대회논문집
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• 한국음향학회 1991년도 학술발표회 논문집
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• pp.45-49
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• 1991

In area of the speech synthesis, the waveform coding with high quality are mainly used to the synthesis by analysis. However, it is difficult to applying the waveform coding to the synthesis by rule, because the parameters of this coding are not classified as either excitation parameters and vocal tract parameters. In this paper, we proposed a new pitch change method that can alter the pitch periods in the waveform coding. The proposed method expands the pitch period by the LPC synthesis method, and then the period is compressed by the waveform halving technique. Thus, it is possible that the waveform coding is carried out the synthesis by rule in speech processing.

• Bulletin of the Korean Chemical Society
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• 제28권9호
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• pp.1493-1498
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• 2007

In this work, alkyl methacrylate-based microgels were synthesized by an experimental design method, and their sebum absorption characteristics were investigated. The results of fractional factorial experimentation indicated that the cross-linking agent content, solvent content, and stirring speed were the main parameters in the synthesis of the microgels. The suitable synthesis conditions were determined by the response surface design method. Through a study of the monomer and solvent effects, it was confirmed that the microgel shows the highest sebum absorption ratio when t-butyl methacrylate is used as a monomer or when acetone is used as a solvent. The optimal microgel synthesis conditions for cosmetic application were determined, and the resulting microgel had a mean particle size of 4.7 μm and a sebum absorption ratio of 435%.

• 대한기계학회논문집A
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• 제22권2호
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• pp.399-407
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• 1998

This paper deals with a method for finding the physical structure of dynamic systems which shows reasonable response to a given specifiation. The method uses only four basic models of bond graph prototypes which have been originally proposed by the authors as a general model for dynamic systems, and then makes its procedure highly physical in the sense that it can synthesize a dynamic system through the structural transformation directly on bond graph models without any mathematical manipulation. Also, it is shown that this method has further advantages in optimizing parameters for an existing system rather than developing design concepts for a new device, the latter being more suitable using the so-called analytical synthesis method introduced by Park and Redfield. One example serves to trace the outlines of the direct synthesis method proposed in this paper for dynamic systems in terms of bond graph prototypes.

• 한국소음진동공학회:학술대회논문집
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• 한국소음진동공학회 2000년도 추계학술대회논문집
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• pp.201-206
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• 2000

In this work transfer function synthesis method based on FRF data of each substructure is investigated for a complex structure composed of many substructures. Though the transfer function synthesis method has superiority to analyze the characteristics of interfaces among substructures effectively, many problems arise in the computation process, especially matrix inversion process. Due to computational problems, the error between the data obtained by test and the predictions through computations is inevitable. So in this paper, computational aspects in the transfer function synthesis method are examined through a steering system problem of passenger car. For the FBS method, frequency response functions of 3 substructures are measured experimentally. Effects of several parameters such as matrix inversion method, connection conditions between substructures and off-diagonal terms on system response are studied numerically.

• BMB Reports
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• 제38권5호
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• pp.517-525
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• 2005

Solid phase peptide synthesis method, which was introduced by Merrifield in 1963, has spawned the concept of combinatorial chemistry. In this review, we summarize the present technologies of solid phase peptide synthesis (SPPS) that are related to combinatorial chemistry. The conventional methods of peptide library synthesis on polymer support are parallel synthesis, split and mix synthesis and reagent mixture synthesis. Combining surface chemistry with the recent technology of microelectronic semiconductor fabrication system, the peptide microarray synthesis methods on a planar solid support are developed, which leads to spatially addressable peptide library. There are two kinds of peptide microarray synthesis methodologies: pre-synthesized peptide immobilization onto a glass or membrane substrate and in situ peptide synthesis by a photolithography or the SPOT method. This review also discusses the application of peptide libraries for high-throughput bioassays, for example, peptide ligand screening for antibody or cell signaling, enzyme substrate and inhibitor screening as well as other applications.