• Fisheries and Aquatic Sciences
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• v.8 no.2
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• pp.90-96
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• 2005

The larvae of Palaemon gravieri were reared in the laboratory at three different temperature regimes ($15^{\circ}C,\;20^{\circ}C,\;and\;25^{\circ}C$) with the salinity ranges (28-32 psu) to understand how temperature and body size influence survival rate, and growth components (molt increment and intermolt period). The optimum temperature for the highest survival rate was $25^{\circ}C$. The intermolt periods consistently increased with an increase in size and instar number; however, the molt increments at successive instars generally decreased with an increase in size and instar number. The shortest intermolt period and the highest larval growth rate both occurred at $25^{\circ}C$. Thus, the optimum temperature for larval survival and growth rate was found to be $25^{\circ}C$ which was the temperature at which the larvae actually appear in nature.

• Journal of Aquaculture
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• v.14 no.1
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• pp.17-27
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• 2001

Effects of temperature (10, 12, 14, 16, 18 and 2$0^{\circ}C$) and salinity (22.0, 24.5, 27.0, 29.5, 32.0 and 34.5 ppt) were studied on incubation period, hatching success, survival and growth of alevin and juvenile seabass, L. japonicus. Embryonic development was accelerated with increasing temperature but it was not influenced by salinity. Hatching success was the highest at 14$^{\circ}C$ and 34.5 ppt. Higher temperature also accelerated the development of mouth opening, absorption of yolk and oil globules, and alevin growth. Survival of the 5-day old Juvenile was accelerated in the following order : 14<16<18<20<12$^{\circ}C$. Rearing experiment of the juvenile for 30-day indicated the faster growth at 13, 20 and 27 ppt than at 34 ppt.

• Journal of Aquaculture
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• v.6 no.3
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• pp.159-171
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• 1993

The effects of environmental factors (density, light, temperature) and live food on growth and survival rate of zoea and mysis of fleshy shrimp, Penaeus chinensis, were examined. The percent survival rates at the culture densities of 200 and 300 larvae per liter were significantly higher than that of 500 larvae per liter (p< 0.05). The percent survival rates at the culture densities of 100, 200, 300 and 500 larvae per liter were $90.3^{ab},\;95.2^a,\;94.3^a\;and\;86.7^b\%$ (p< 0.05). The effects of light conditions with diatom premix diet showed that continuous light was the best for the survival rate, $96.5\%$. Natural light and continuous darkness showed the survival rates $79.0\%\;and\;4.3\%$, respectively. On the rearing temperature, the survival rates were $97.3\%\;at\;19^{\circ}C\;and\;96.7\%\;at\;22^{\circ}C$ with little difference but the higher temperature resulted in faster growth. The survival rate was $51.6\%;at\;25^{\circ}C$ thus showing significant decrease compared to $19^{\circ}C\;and\;22^{\circ}C$. At this temperature the growth was also suffered. With regard to dietary value of live food, both the best survival rate and the fastest growth were obtained when 6 mixed species of diatom (A. normaii, C. simplex, N closterium, P. tricornutum, S. costatum and T. weissflogii) were provided. When single species was supplied the best survival rate $(82.1\%)$ was obtained with S. costatum, but the other species resulted in much inferior survival rates $(below\;80.0\%)$ and poor growth.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.45 no.4
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• pp.251-259
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• 2009

Swimming crabs, Portunus trituberculatus(Miers) are commercially important off the coasts of Korea, Japan and China. Harvest of swimming crabs has been fluctuated along their distribution ranges. Fluctuations in the interannual harvest of swimming crabs may be correlated with the survival rate during the larval period. The survival rates, intermolt periods, and growth of larval swimming crabs were investigated in the laboratory. Larval swimming crabs are released and undergo development from April to August off the western coast of Korea in the Yellow Sea. Sea surface temperatures off the western coast of Korea during the larval season were used for the laboratory experiments, and ranged from 22 to 26${^{\circ}C}$. Larvae were individually cultured at four different temperatures, 22${^{\circ}C}$, 24${^{\circ}C}$, 26${^{\circ}C}$, and 28${^{\circ}C}$. Zoea molted to megalopa at all temperatures and developed to the first crab stage at 24${^{\circ}C}$, 26${^{\circ}C}$, and 28${^{\circ}C}$. Survival rates from zoea I to the first crab stage increased with increasing temperatures. Intermolt period and the growth rate of the mean carapace length were inversely correlated with temperature. Our research helps understand the changes in survival rate and growth of larval swimming crabs resulting from changing oceanic temperatures. Further, our study suggests that the fluctuations in fishery harvest of swimming crabs off the coast of Korea may be related to changes in larval survival affected by changing ocean conditions.

• The Korean Journal of Malacology
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• v.19 no.1
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• pp.25-32
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• 2003

To know the effects of the red tide and toxic dinoflagellates on survival and growth of larvae of the mussel, Mytilus galloprovincialis, laboratory experiments were conducted by incubating larvae with either unialgal culture of 4 dinoflagellate species (Amphidinium carterae, Prorocentrum triestinum, Gymnodinium impudicum, or Akashiwo sanguinea) or a standard food (Isochrysis galbana) for 10 days. The survival of larvae was higher than 80% when the food was A. carterae, G. impudicum, or A. sanguinea. The lowest survival (20%) was found when the food was P. triestinum. When the food was P. triestinum, the survival of larvae rapidly decreased from 87% at day 4 down to ca. 50% at day 6, and 20% at day 10. This implies that the larval population of M. galloprovincialis can seriously be affected if they are exposed to the red tide water dominated by P. triestinum for more than 4 days. Shell length of larvae either increased or decreased according to the food species. When the food was A. carterae, G. impudicum, or A. sanguinea, shell length of larvae increased. But, it decreased when the food was P. triestinum. Though shell length increased in 3 treatments, the daily increments (0.63 $\mu$ m for A. carterae, 0.46 m for $\mu$ G. impudicum, and 1.10 m for $\mu$ A. sanguinea) were smaller than that of the standard food (3.79 m for $\mu$ I. galbana). Correlation analyses chowed that the change in shell length was not significant when the food was A. carterae or G. impudicum. Therefore, all of 4 dinoflagellates affected the growth of M. galloprovincialis larvae: growth was negative for P. triestinum, nil for A. carterae and G. impudicum, and positive but lower than standard food for A. sanguinea. These imply that the dinoflagellates are less valuable as foods for M. galloprovincialis larvae. So, decreased growth rate of larvae is expected during red tides, which will consequently cause delayed metamorphosis or failure to recruitment to the adult populations. In considering the harmful effects of red tides on the aquatic ecosystem, not only the effects on adult populations of fish and shellfish, but also the effects on larval populations should be included.

• Journal of Korean Society of Forest Science
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• v.88 no.4
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• pp.485-489
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• 1999

The germination percentages of Quercus hybrid seeds produced in 1994 using stored pollens were low. The survival rate and height growth within 2 years differed according to crossing combinations. The survival rate and growth of seedlings from hybrid seeds produced in 1991 and 1993 were investigated. The survival rates of five-year-old and three-year-old seedlings were from 71 to 100% and from 33 to 100%, respectively. The survival rates differed according to cross combinations. The mortality of seedlings increased in proportion to the increase of percentages of dwarf seedlings. The growth of five-year-old seedlings, of which mother tree was Q. aliena, was the best. The growth of seedlings produced by artificial crossing tended to be worse than that of natural crossing.

• Journal of fish pathology
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• v.17 no.1
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• pp.67-74
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• 2004

This study investigates the influence of waterborne ammonia and nitrite on the zoea and megalopa stage larvae of tiger crab, Orithyia sinica under laboratory condition, focusing on the effects on survival and growth as deleterious responses of toxicant. Survival rate of zoea stage larvae exposed to control levels, and to 5, 10, 20 and 50 mg/L total ammonia-N, using a continuous flow system for 20 days was 80, 77, 45, 40 and 37%, respectively. Growth rate of zoea stage larvae exposed to 20 and 50 mg/L total ammonia was significantly lower than in controls after 20 days (P< 0.05). Survival rate and growth rate of megalopa stage larvae exposed to ammonia also decreased at greater than 10 and 50 mg/L, respectively. In the nitrite exposure experiment with zoea and megalopa stage larvae of tiger crab, survival rate was decreased in a concentration and exposure period-dependent way. The growth rate of zoea and megalopa stage larvae of tiger crab exposed to nitrite decreased at greater than 150 mg/L nitrite concentration.

• Clinical and Experimental Reproductive Medicine
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• v.32 no.2
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• pp.91-99
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• 2005

Objective: To define an appropriate vitrification condition of preantral follicle that yields high survival and to evaluate growth and ovulation rate of mouse follicles during in vitro culture after vitrification. Methods: Preantral follicles were isolated mechanically from mouse ovaries that were surgically recovered from mice aged 14 days. Retrieved preantral follicles were placed in EG (Ethylene Glycol) for 2, 5, 10 minutes and transferred to EFS-40 (40% EG, 18% Ficoll-70, 0.5 M sucrose) for 0.5, 1, 2 minutes. And then, preantral follicles were placed onto an EM grid and submerged immediately in liquid nitrogen. Thawing was carried out at room temperature. After defining the most appropriate vitrification condition that yields high survival, in vitro growth and ovulation rate of follicles were evaluated. Results: Appropriate vitrification condition that yield high survival rate ($83.2{\pm}2.1%$) of preantral follicle was EG for 5 minutes and EFS-40 for 0.5 minutes. In vitro survival rate of the vitrified preantral follicles were $85.5{\pm}0.5%$, $67.9{\pm}0.8%$ and $40.2{\pm}0.5%$ on day 2, 6 and 10. And in vitro growth of the vitrified preantral follicles were $107.1{\pm}16.1{\mu}m$, $117.1{\pm}18.4{\mu}m$, $178.4{\pm}45.6{\mu}m$ and $325.4{\pm}54.4{\mu}m$ on day 0, 2, 6 and 10. Although in vitro survival rate and growth of vitrified preantral follicles were lower than that of non-vitrified preantral follicles, the patterns of survival and growth were similar in vitrified and non-vitrified preantral follicles. The ovulation rate of antral follicles that was grown from vitrified preantral follicles was $32.6{\pm}1.2%$. Conclusion: Vitrified preantral follicles could be grown to antral sizes, and mature oocytes that can be used for IVF-ET programs were produced successfully. These data suggest that cryopreservation of preantral follicle by vitrification can be used for the preservation of the fertility.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.49 no.3
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• pp.343-350
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• 2016

The sea cucumber Apostichopus japonicus is a commercially valuable aquaculture species in Korea. Aquaculture species require specific nursery culture conditions to increase survival and growth rates. Sea cucumbers hibernate during the high temperatures of summer and during the low temperatures of winter, and suboptimal temperature conditions decrease sea cucumber growth and survival rates. The natural South Korean environment is very unfavorable for culturing sea cucumber; therefore, developing a recirculating aquaculture system (RAS) capable of breeding and growing sea cucumber year-round is necessary. The aim of this study was to investigate growth performance of juvenile sea cucumber in a RAS. Growth and survival rates of juvenile sea cucumber were high during our 24-week experiment. Sea cucumber survival rates were 87.8-93.3%, and specific growth rates were 0.4689-0.7846.

• Journal of Practical Agriculture & Fisheries Research
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• v.13 no.1
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• pp.115-129
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• 2011

This study was performed to investigate the effects of food organism and water quality on the mass production of larval red marbled rockfish, Sebastiscus tertius. When rotifer cultured with different food stuffs were supplied to the larvae, rotifer cultured with concentrated Chlorella provided the highest in total length 11.9 mm and survival rate was the highest by 47.0% fed that cultured with ω-yeast Growth and survival rate of larvae fed on enriched rotifer with ω-yeast and super selco, super selco of enriched treatment showed that the best results not only growth but also survival rate. For instance of Artemia, the growth was not significantly different with other treatment but the survival rate was significantly higher than others. The growth of larvae fed with different diets indicated that rotifer+Artemia+commercial diet treatment was the highest on the total length and body weight 18.40 mm and 18.1 mg, respectively, whether the survival rate was rotifer+Artemia treatment was the highest as 57.5%. The most effective density of Chlorella was 10×10⁵ cells/ml for growth of larvae. That density showed that total length and body weight were 11.83 mm and 14.3 mg, respectively. The survival rate was the most effective for 20×10⁵ cells/ml of Chlorella density. That density represented the highest survival rate as 54.5%. Comparing of PSB adding effect, PSB density 15×10⁵ cells/ml was the most effective for growth of larvae and but for survival rate PSB density 10×10⁵ cells/ml was the highest as 61.0%.