• Asian Pacific Journal of Cancer Prevention
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• 제13권7호
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• pp.3089-3097
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• 2012

Background and Aims: Vascular endothelial growth factor (VEGF) is a potential prognostic biomarker for patients with resected gastric cancer. However, its role remains controversial. The objective of this study was to conduct a systematic review and meta-analysis of published literature. Methods: Relevant literature was identified using Medline and survival data from published studies were collected following a methodological assessment. Quality assessment of eligible studies and meta-analysis of hazard ratio (HR) were performed to review the correlation of VEGF overexpression with survival and recurrence in patients with gastric cancer. Results: Our meta-analysis included 44 published studies with 4,794 resected patients. VEGF subtype for the prediction of overall survival (OS) included tissue VEGF (HR=2.13, 95% CI 1.71-2.65), circulating VEGF (HR=4.22, 95% CI 2.47-7.18), tissue VEGF-C (HR=2.21, 95% CI 1.58-3.09), tissue VEGF-D (HR=1.73, 95% CI 1.25-2.40). Subgroup analysis showed that HRs of tissue VEGF for OS were, 1.78 (95% CI 0.90-3.51) and 2.31 (95% CI 1.82-2.93) in non-Asians and Asians, respectively. The meta-analysis was also conducted for disease free survival (DFS) and disease specific survival (DSS). Conclusion: Positive expression of tissue VEGF, circulating VEGF, VEGF-C and VEGF-D were all associated with poor prognosis in resected gastric cancer. However, VEGF demonstrated no significant prognostic value for non-Asian populations. Circulating VEGF may be better than tissue VEGF in predicting prognosis.

• Asian Pacific Journal of Cancer Prevention
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• 제13권3호
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• pp.1059-1063
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• 2012

Objective: Angiogenesis represents a key element in the pathogenesis of malignancy. There are no robust data on prognostic factors for overall survival (OS) in patients with metastatic colorectal cancer treated with vascular endothelial growth factor (VEGF)-targeted therapy. The present study was conducted to establish a prognostic model for patients using an oxaliplatin-based or irinotecan-based chemotherapy plus bevacizumab in metastatic colorectal cancer. Methods: Baseline characteristics and outcomes on 170 patients treated with FOLFIRI or XELOX plus anti-VEGF therapy-naive metastatic colorectal cancer were collected from three Turkey cancer centers. Cox proportional hazards regression was used to identify independent prognostic factors for OS. Results: The median OS for the whole cohort was 19 months (95% CI, 14.3 to 23.6 months). Three of the seven adverse prognostic factors according to the Anatolian Society of Medical Oncology (ASMO) were independent predictors of short survival: serum lactate dehydrogenase (LDH) greater than the upper limit of normal (ULN; p<0.001); neutrophils greater than the ULN (p<0.0014); and progression free survival (PFS) less than 6 months (p =0.001). Conclusion: Serum LDH and neutrophil levels were the main prognostic factors in predicting survival, followed by PFS. This model validates incorporation of components of the ASMO model into patient care and clinical trials that use VEGF-targeting agents.

• Asian Pacific Journal of Cancer Prevention
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• 제14권2호
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• pp.645-649
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• 2013

Objective: The prognostic role of vascular endothelial growth factor (VEGF) in bladder cancer remains controversial. This meta-analysis aimed to explore any association between overexpression and survival outcomes. Methods: We systematically searched for studies investigating the relationships between VEGF expression and outcome of bladder cancer patients. Study quality was assessed using the Newcastle-Ottawa Scale. After careful review, survival data were extracted from eligible studies. A meta-analysis was performed to generate combined hazard ratios (HRs) for overall survival (OS), disease-free survival (DFS) and disease-specific survival (DSS). Results: A total of 1,285 patients from 11 studies were included in the analysis. Our results showed that tissue VEGF overexpression in patients with bladder cancer was associated with poor prognosis in terms of OS (HR, 1.843; 95% CI, 1.231-2.759; P = 0.003), DFS (HR, 1.498; 95% CI, 1.255-1.787; P = 0.000) and DSS (HR, 1.562; 95% CI, 0.996-1.00; P = 0.052), though the difference for DSS was not statistically significant. In addition, there was no evidence of publication bias as suggested by Begg's and Egger's tests except for DFS (Begg's test, P = 0.221; Egger's test, P = 0.018). Conclusion: The present meta-analysis indicated elevated VEGF expression to be associated with a poor prognosis in patients with bladder cancer.

• Biomolecules & Therapeutics
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• 제26권5호
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• pp.464-473
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• 2018

Cripto is a small glycosylphosphatidylinositol-anchored signaling protein that can detach from the anchored membrane and stimulate proliferation, migration, differentiation, vascularization, and angiogenesis. In the present study, we demonstrated that Cripto positively affected proliferation and survival of mesenchymal stem cells (MSCs) without affecting multipotency. Cripto also increased expression of phosphorylated janus kinase 2 (p-JAK2), phosphorylated signal transducer and activator of transcription 3 (p-STAT3), 78 kDa glucose-regulated protein (GRP78), c-Myc, and cyclin D1. Notably, treatment with an anti-GRP78 antibody blocked these effects. In addition, pretreatment with STAT3 short interfering RNA (siRNA) inhibited the increase in p-JAK2, c-Myc, cyclin D1, and BCL3 levels caused by Cripto and attenuated the pro-survival action of Cripto on MSCs. We also found that incubation with Cripto protected MSCs from apoptosis caused by hypoxia or $H_2O_2$ exposure, and the level of caspase-3 decreased by the Cripto-induced expression of B-cell lymphoma 3-encoded protein (BCL3). These effects were sensitive to down-regulation of BCL3 expression by BCL3 siRNA. Finally, we showed that Cripto enhanced expression levels of vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), and hepatocyte growth factor (HGF). In summary, our results demonstrated that Cripto activated a novel biochemical cascade that potentiated MSC proliferation and survival. This cascade relied on phosphorylation of JAK2 and STAT3 and was regulated by GRP78. Our findings may facilitate clinical applications of MSCs, as these cells may benefit from positive effects of Cripto on their survival and biological properties.

• 한국양식학회지
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• 제12권3호
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• pp.213-220
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• 1999

This study was performed to investigate the effects of water temperature and stocking density and rotifer density on the mass production of larval red marbled rockfish, Sebastiscus tertius. Total length of the marbled rockfish larva grown in $23^{\circ}C$ of water temperature were 10.80mm, which was the best, while those grown in $29^{\circ}C$ of water temperature were 6.28mm, which was the lowest. The survival rates of red marbled rockfish larvae grown in $20^{\circ}C$ and $23^{\circ}C$ were 24.0% and 18.5%, respectively. However, the survival rate of red marbled rockfish larvae grown in $26^{\circ}C$ was $7^{\circ}C$ and no larvae survived in $29^{\circ}C$. The total length of red marvled rockfish larva stocked at the density of 5 and 10 larvaper liter of water were high, which were 11.52 mm and 11.22 mm, respectively, but those stocked at the density of 30 larva per liter of water were 7.55mm, which was the lowest. The survival rate of red marbled rockfish larva stocked at the density of 2.5 larva per liter of water was 52.0%, which was the best, but the lowest, 18.0% for the red marbled rockfish larva stocked at the density of 30 larva per liter. There was a trend toward decrease in survival rate of red marbled rockfish larva as their stocking density increased. The survival rates of red marbled rockfish larva fed rotifers at the density of 10 and 5 individuals per ml were high, 48.5% and 48.0%, and their total lengths were 11.92 and 11.89mm, respectively, which grew relatively fast. The survival rate and the total length of red marbled rockfish larva fed rotifers at the density of 30 individuals per ml were the lowest, which were 8.40mm and 21.5%, respectively. Also, red marbled rockfish larva fed rotifers at the density of more than 20 individuals per ml achieved poor survival and growth. These results indicated that the proper conditions for the mass production of larval red marbled rockfish were $23^{\circ}C$ of water temperature, fish larva stocking density of 5 to 10 larva per liter of water and rotifers density of 5 to 10 individuals per ml as live feed.

• 한국어병학회지
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• 제15권3호
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• pp.99-104
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• 2002

넙치의 생존, 성장 및 대사에 미치는 납의 만성적 독성을 파악하기 위하여 6주 동안 납 0, 80, 150, 230, 420 ${\mu}g/L$농도에 노출시켜 실험을 실시하였다. 넙치의 생존율은 납 농도 230 ${\mu}g/L$이상에서 유의한 감소를 나타냈다. 넙치의 성장률과 사료효율은 납 농도가 증가할수록 감소를 하여 150 ${\mu}g/L$이상의 농도에서는 대조구에 비하여 유의한 감소를 나타냈다. 넙치의 대사율은 납 농도 80, 150, 230, 420 ${\mu}g/L$에서 대조구보다 각각 21.4, 23.8, 36.8, 44.9 %가 저하했으며, 80 ${\mu}g$/L이상의 농도에서는 유의한 감소를 나타냈다.

• Environmental Analysis Health and Toxicology
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• 제25권2호
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• pp.161-168
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• 2010

The observed the toxic effect of WSF (Water Soluble Fraction) on development time, development rate, attachment rate, survival rate and growth of the larva during the early stage of the Pacific oyster, Crassostrea gigas. The time required early juvenile from the fertilized eggs exposed to crude oil (WSF) was about 13 hours in control group. But that of the crude oil dose group takes 12 hours, with shorter compared to control group. Development and attachment rate of the oyster significantly higher in 0.4 and 0.8 mg/L WSF compared to control group (P<0.05) but significantly lower more than 1.6 mg/L WSF (P<0.05). Survival rate of the oyster larva significantly higher in 0.4 and 0.8 mg/L WSF compared to control group (P<0.05) but significantly lower more than 2.4 mg/L WSF (P<0.05). Survival rate of spot showed no significantly difference compared to control group (P>0.05). Shell growth of the oyster were significantly lower more than 1.6 mg/L WSF compared control group (P<0.05). The effect concentration of crude oil showed that more 0.4 mg/L in development and attachment rate and more 1.6 mg/L in growth. Also, chronic exposure of crude oil even effect also in low concentration.

• 한국수산과학회지
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• 제32권3호
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• pp.261-265
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• 1999

시안의 만성적 조건에서 이스라엘 잉어의 생존, 섭이 및 성장에 미치는 독성영향을 파악하기 위하여 유수식방법에 의해 6주 동안 실험을 실시하였다. 시안의 만성적 조건하에 노출시킨 이스라엘잉어의 생존율은 시안농도 $151{\mu}$g/$\ell$이상에서 유의한 감소를 나타내었고, 실험종료시 시안농도 151, $289{\mu}$g/$\ell$에서 대조구에 비해 각각 15.2, $24.5\%$가 감소하였다. 섭이율은 시안농도 $151{\mu}$g/$\ell$에서는 3주, $289{\mu}$g/$\ell$에서는 1주 후부터 실험종료까지, 사료효율은 시안농도 $151{\mu}$g/$\ell$이상에서 2주 후부터 실험종료시까지 대조구에 비해 유의한 감소가 인정되었다. 성장률 및 일일성장율은 시안농도 $73{\mu}$g/$\ell$에서 6주 후에, 시안 농도 $151{\mu}$g/$\ell$이상에서는 2주 후부터 실험 종료시가지 유의한 감소를 나타냈다.

• 한국양식학회지
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• 제11권3호
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• pp.303-310
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• 1998

난황형성 단계에 있는 암컷 어미에게 갑상선 호르몬인 3,5,3'-triiodo-L-thyronine ($T_3$)를 20 mg $T_3$/kg 체중으로 주사하여 출산된 조피볼락 자어를 대조구인 sham구 및 control구 자어와 함께 30일간 사육하면서 성장과 생존율을 비교하였다. 자어에게는 출산후 5일째까지 rotifer를 공급하였고, 6~15일까지 rotifer와 Artemia nauplii를, 이후 30일까지 Artemia nauplii와 넙치용 배합사료를 혼합공급하였다. 출산후 자어의 성장은 대조구들에 비해 $T_3$구에서 유의하게 빨랐으며, 생존율 역시 T하(3)구가 대조구들 보다 유의하게 높았다. 실험종료시 치어의 비만도는 $T_3$구가 control구에 비해 다소 낮았고, sham구에 비해서는 유의하게 낮았다. 이상의 결과, 조피볼락에 대한 $T_3$의 모체주사는 모체에서 유래된 외인성$T_3$가 초기 출산자어의 생리활성을 증진시켜, 자치어의 성장과 생존율 향상에 도움을 줄 수 있는 것으로 평가되었다.

• 환경생물
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• 제22권1호
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• pp.153-158
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• 2004

염분에 대한 두줄망둑의 내성범위를 파악하기 위한 일환으로 이들의 생존, 성장 및 산소소비에 미치는 염분의 영향을 40일간 사육실험을 통하여 검토하였다. 두줄망둑의 생존율은 염분 10.1$\textperthousand$ 이상에서 90%이상을 나타냈으나, 염분 3.4$\textperthousand$ 이하에서는 유의한 감소를 나타냈고, 담수에서는 40일째 32.5%의 생존율을 보였다. 체장과 체중의 증가 및 성장률은 13.4∼33.6%o의 염분 범위에서 유사한 경향을 나타냈으나, 염분 6.7$\textperthousand$ 이하에서 유의한 감소가 관찰되었다. 산소소비는 염분 10.1%o 이하에서 유의한 감소가 관찰되었고, 염분 10.1, 6.7 및 3.4$\textperthousand$ 에서는 가장 높은 산소소비를 나타낸 27.4$\textperthousand$ (6.67 ml $O_2g^{-1}$ dry weight)의 염분에 비해 각각 28.4, 30.9 및 37.4%가 감소하였고, 담수에서는 52.3%의 감소를 나타냈다. 이러한 결과는 두줄망둑이 낮은 염분에 노출되었을 때, 그들의 생존, 성장 및 산소소비율이 저하한다는 사실을 지적하고 있으며, 하구 수역과 같은 저염분 수역은 두줄망둑의 분포 및 개체수에 잠재적으로 영향을 미칠 수 있다는 것을 암시한다.