• Transactions of the Korean Society of Automotive Engineers
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• v.6 no.4
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• pp.141-150
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• 1998

The measurement of strains in stamped sheet metal is essential to the design and manufacture of sound sheet metal products. The measured strains can also be used in verifying the reliability of the computer analysis such as finite element analysis. In most engineering applications, strains are measured from the deformed square grids or deformed circular grids in comparison with the initial undeformed grids. In such a case, however, strains are averaged in each grid and the localized strain in a region smaller than a grid size can not be measured. In the present study, the B-spline surface interpolation technique is introduced in order to measure the strains more exactly and effectively. The strains calculated by using the surface interpolation technique are compared with the strains calculated from the three-noded grids as well as with the finite element analysis.

• Journal of Microbiology and Biotechnology
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• v.26 no.5
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• pp.846-853
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• 2016

We displayed four types of Solanum nigrum metallothionein (SMT) for the first time on the surface of Saccharomyces cerevisiae using an α-agglutinin-based display system. The SMT genes were amplified by RT-PCR. The plasmid pYES2 was used to construct the expression vector. Transformed yeast strains were confirmed by PCR amplification and custom sequencing. Surface-expressed metallothioneins were indirectly indicated by the enhanced cadmium sorption capacity. Flame atomic absorption spectrophotometry was used to examine the concentration of Cd²⁺ in this study. The transformed yeast strains showed much higher resistance ability to Cd²⁺ compared with the control. Strikingly, their Cd²⁺ accumulation was almost twice as much as that of the wild-type yeast cells. Furthermore, surface-engineered yeast strains could effectively adsorb ultra-trace cadmium and accumulate Cd²⁺ under a wide range of pH levels, from 3 to 7, without disturbing the Cu²⁺ and Hg²⁺. Four types of surfaceengineered Saccharomyces cerevisiae strains were constructed and they could be used to purify Cd²⁺-contaminated water and adsorb ultra-trace cadmium effectively. The surface-engineered Saccharomyces cerevisiae strains would be useful tools for the bioremediation and biosorption of environmental cadmium contaminants.

• Journal of the Korean Society for Precision Engineering
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• v.10 no.3
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• pp.47-56
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• 1993

Typical plastic strains in the machined surface are very difficult to measure, since they are located within a very short distance from the surface and they change very rapidly. There is an alternative way to determine the residual strain in plastically deformed materials by measuring the grain size after a subsequent recrystallization precess. Although, this technique has been successfully applied by several researchers to find the plastic zone around notches and cracks in various materials and welding beads, few works have been reported using the recrystallization method to determine the residual strains in machined surface. Therefore, the purpose of this investigation is to explore the effectiveness of the recrystallization technique in machining applications and in particular, to find the effect of cutting parameters, i.e., depth of cut, rake angle, on the plastic strains. As the result, the recrystallization technique was succesfully applid to determine the plastic strain in machined surface.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 1991.11a
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• pp.54-58
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• 1991

It is well known that metal cutting leaves a plastically deformed layer in the machined surface. This residual phenomenon affects in various forms the physical properties of machined components such as the fatigue strength, the dimensional instability, microcracks, and the stress corrosion cracking. These physical properties, so called surface integrity, are very important for designing highly stressed and critically loaded components. Typical plastic strains in the machined surface are very difficult to measure, since they are located within a very short distance from the surface and they change very rapidly. There is an alternative way to determine the residual strain in plastically deformed materials by measuring the grain size after a subsequent recrystallization process. Although, this technique has been successfully applied by several researchers to find the plastic zone around notches and cracks in various materials and welding beads, few works have been reported using the recrystallization method to determine the residual strains in machined surface. Therefore, the purpose of this investigation Is to explore the effectiveness of the recrystallization technique in machining applications, and in particular, to find the effect of cutting parameters, i.e., depth of cut and rake angle on the plastic strains.

• Proceedings of the Korean Society For Composite Materials Conference
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• 2003.10a
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• pp.150-153
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• 2003

Longitudinal strains({$varepsilon}_x$) of the core and skin layers in glass fiber reinforced plastic(GFRP) cross-ply composite laminates have been studied using the embedded optical fiber sensor of totally-reflected extrinsic Fabry-Perot interferometer(TR-EFPI). Foil-type strain gauges bonded on both the upper and lower surfaces were used for the measurement of the surface strains. Both TR-EFPI sensor and strain gauge bonded on the specimen surface showed excellent agreement within -0.0086 ~ +0.0302% strain. It was shown that values of {$varepsilon}_x$ in the interior of the surface layer and the core layer measured by embedded TR-EFPI sensor was significantly higher than that of the specimen surface measured by strain gauges. The experimental results were ascertained with finite element analysis. Embedded TR-EFPI optical fiber sensor could measure accurately the internal strains which were different from the surface.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.13 no.3
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• pp.163-172
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• 1993

A probabilistic approach for evaluation of prediction of the strains using Lade's single surface constitutive model was employed, based on first-order approximate mean and variance. Several experiments such as isotropic compression and drained triaxial compression tests were conducted to examine the variabilities of soil parameters for Lade's model. By taking into account the results of the experimental data such as mean values and standard deviations of soil parameter's, a new probabilistic approach, which explains the uncertainty of computed strains, is applied. The magnitude of the COV for each parameter and the correlation coefficient between the two parameters can be effectively used for reducing the number of the parameters for the model. It is concluded that Lade's single surface constitutive model is surperior model for the prediction of the strain, because the COV of strains is under the "0.51".

• Journal of Animal Science and Technology
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• v.59 no.2
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• pp.4.1-4.5
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• 2017

Background: Biofilms were the third-dimensional structure in the solid surface of bacteria. Bacterial biofilms were difficult to control by host defenses and antibiotic therapies. Escherichia coli (E. coli) and Salmonella were popular pathogenic bacteria that live in human and animal intestines. Essential oils are aromatic oily liquids from plant materials and well known for their antibacterial activities. Method: This study was conducted to determine effect of essential oil on anti-biological biofilm formation of E. coli and Salmonella strains in in vitro experiment. Two kinds of bacterial strains were separated from 0.2 g pig feces. Bacterial strains were distributed in 24 plates per treatment and each plates as a replication. The sample was coated with a Bacterial biofilm formation was. Result: Photographic result, Escherichia coli (E. coli) and Salmonella bacteria colony surface were thick smooth surface in control. However, colony surface in blended and single essential oil treatment has shown crack surface layer compared with colony surfaces in control. Conclusion: In conclusion, this study could confirm that essential oils have some interesting effect on anti-biofilm formation of E. coli and Salmonella strains from pig feces.

• The Journal of Korean Academy of Prosthodontics
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• v.34 no.2
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• pp.363-372
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• 1996

A common site of fracture in maxillary complete denture is on the anteroposterior midline that coincides with the notch for relief of the labial frenum. Various approaches to reduce the incidence of this type of fracture have been suggested. The most widely used technique is the reinforcement of acrylic resin denture base with several solid metal forms. But few comparative studies on the efficacy of metal reinforcements have been reported. This study was conducted to compare reinforcing effects of commonly available metal reinforcements, which include wire, metal mesh embedded in the denture base and metal plate affixed to the impression surface of denture base by silicoating technique. This was load on the posterior. The strain gauges were oriented perpendicular to the anteroposterior midline of maxillary polished denture surface at one labial and the four palatal sites Non-renforced denture was used as control. The results were as follows : 1. In the non-reinforced denture group, only tensile strains on the palatal polished surface were observed. The tensile strains decreased in the order of incisive papilla, posterior denture border area, mid palatal area and rugae area. Compressive strain was observed on the labial polished surface. 2. As compared with the non-reinforced denture group, the metal plate or the metal mesh reinforced denture groups showed reduced palatal tensile strains,and the metal mesh reinforcement had a better reinforcing effect than the metal plate. But both reinforced denture groups showed no difference in the amount of compressive strain on the labial polished surface when compared to the non-reinforced denture group. 3. The metal wire positioned just above the labial notch decreased the compressive strain on the labial polished surface. But the presence of metal wires in the palatal polished surface caused increase in tensile strains in the area.

• Microbiology and Biotechnology Letters
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• v.4 no.4
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• pp.138-144
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• 1976

five strains of Streptomyces spp. with high Productivity of glucose isomerase (15-30 units/ml) were obtained among 280 microbial strains isolated from 150 soil samples. These strains produced glucose isomerase with xylose as an inducer. These 5 strains were also identified to be different strains of Streptomyces spp.：streptomyces sp. K-14, K-53, K-71, K-77 and K-733. It was found that Streptomyces sp. K-14 produced the highest enzyme activity. The spore chains of these strains were rectiflexible and spore surface was smooth except Steptomyces sp. K-77 and K-733, with spiny surface.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.5
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• pp.751-755
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• 2006

Dadih is Indonesian traditional fermented buffalo milk believed by the natives to have beneficial effects on human health. This may be due to the probiotic properties possessed by the lactic acid bacteria (LAB) involved in its fermentation process. It was discovered that ten strains of dadih lactic isolates possessed some probiotic properties in vitro. In this study, the adhesion properties of dadih LAB, in comparison with documented probiotic strains, were investigated in vitro by using mucin extracted from human faeces and Caco-2 cells as the models for human intestinal mucosal surface and intestinal cells respectively. The adhesion results showed the distinction of Lactobacillus reuteri IS-27560 in adhering to both mucus layer and Caco-2 cells. The competition assay for adhesion to the mucus layer between dadih LAB and selected pathogens indicated the competence of Lactococcus lactis IS-16183 and Lactobacillus rhamnosus IS-7257 in significantly inhibiting the adhesion of Escherichia coli O157:H7. Accordingly, these two strains may be potential candidates for use as probiotic strains. Overall, the adhesion properties of all dadih LAB strains were relatively comparable to that of Lactobacillus casei Shirota and Lactobacillus rhamnosus GG, the documented probiotic strains.