• 제목/요약/키워드: surface localization

검색결과 199건 처리시간 0.03초

장대끈끈이주걱 분비모의 미세구조와 peroxidase 활성 (Ultrastructure and Activity Pattern of Peroxidase in Secretory Trichomes of Drosera capensis)

  • 김은수;오승은;유성철
    • Applied Microscopy
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    • 제28권3호
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    • pp.399-414
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    • 1998
  • Glandular trichomes present on the leaf surface of Drosera capensis were examined using transmission electron microscopy. A large number of stalked glands exist on the adaxial surfaces of the leaf blade. The secretory head is composed of two layers of secretory cells, one layer of middle cells, and the inner tracheids. The secretory cells contain rough endoplasmic reticulum, mitochondria, plastids, Golgi apparatus, and vacuoles. The secretory cells show prominent cell wall ingrowth, and thick cuticle restricted on the subcuticular wall. Frequently, the cuticle has some pores, canal-like structures, showing electron -dense granules being penetrated through them. Ultrastructural localization using diaminobenzidine showed the electron-dense deposits in the vacuole. No peroxidase activity was seen in the cell wall and cytolasm. The activity of peroxidase (POX) isozymes in Drosera which isoelectric point (pI) is 3.6 and some anionic POX isozymes which pIs are laid between 3.6 and 4.6 were especially increased according to the development and the formation of glandular trichomes. Also, the activity of some POX isozymes which isoelectric points are laid between 4.6 and 5.1 were increased in the regions of leaves which has trichomes.

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Ultrasound-guided Evacuation of Spontaneous Intracerebral Hemorrhage in Basal Ganglia

  • Park, Seong-Keun;Lee, Jung-Kil;Shin, Seung-Ryeol;Lee, Je-Hyuk
    • Journal of Korean Neurosurgical Society
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    • 제37권3호
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    • pp.197-200
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    • 2005
  • Objective: Ultrasound can be used in the treatment of large intracerebral hematoma. The authors present our experiences with Ultrasound-guided catheter placement for lysis and drainage of ganglionic hematoma, with emphasis on technical aspects. Methods: The authors applied real-time ultrasonography for the aspiration of intracerebral hematoma in 6cases. Ultrasound-guided aspiration via a burrhole was performed under local anesthesia. We selected a temporal entry point instead of the frequently used precoronal approach in ganglionic hematoma. A burrhole was made 4 to 6cm posterior from posterior border of frontal process of the zygomatic bone at the level of 4 to 5cm above the external auditory meatus. Results: In all patients, the catheter was placed accurately into the hematoma target. All patients were irrigated with urokinase once to three times a day. The catheter could be removed within two or three days. The mean hematoma volume was reduced from initially 32mL to 5mL in an average of two days. There were no intraoperative complications related to the use of real-time ultrasonography and no postoperative infections were noted. Conclusion: Ultrasound allows an easy and precise localization of the hematoma and the distance from the surface to the target can be calculated. Ultrasound-guided catheter placement for fibrinolysis and hematoma drainage is a simple and safe procedure.

에지의 구조적 영역정보를 이용한 에지검출 (Edge Detection Using the Information of Edge Structural Regions)

  • 김수겸;박중순;최정희
    • Journal of Advanced Marine Engineering and Technology
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    • 제24권2호
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    • pp.82-89
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    • 2000
  • 에지검출은 영상인식의 첫 단계임을 동시에 영상인식의 성능을 좌우하는 아주 중요한 단계이다. 기존의 기울기연산자나 표면접합에 의한 에지검출과 달리 본 논문에서는 에지의 영역정보를 이용한 에지검출 알고리즘을 제시한다. 먼저 에지의 적합한 위치, 에지의 두께 그리고 에지의 길이에 대한 정의를 제시하고, 제시된 에지정의를 기본으로한 12개의 에지검출 윈도우와 알고리즘을 제안하였다. 제안된 12개의 윈도우는 모든 형태의 에지를 추출할 수 있는 에지검출윈도우로써 일반적으로 많이 사용되고 있는 기울기 연산자나 0점교차 연산자인 LoG 연산자보다 좋은 에지검출 성능을 보여 주었다.

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국내 토양공정법과 산처리 방법의 비교: 최종 pH와 용출률의 관계

  • 이평구;최선규;오창환;이재영
    • 한국지하수토양환경학회:학술대회논문집
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    • 한국지하수토양환경학회 2000년도 창립총회 및 춘계학술발표회
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    • pp.46-51
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    • 2000
  • Environmental quality regulations are used to establish the maximum permissible concentrations of heavy metals before a site can be deemed to be polluted or contaminated. This paper compares the results obtained by 0.IN HCl extraction and acid extraction method(HNO$_3$+HClO$_4$.HCl). The leaching efficiency of 0.1N HCl extraction is directly proportional to the final pH of leachate, due to the different solubilities of the heavy metals at different pH values, The severe differences between 0.1N HCl-extractable and total metal contents result mainly from the buffering effect of carbonates, present in sediment. samples. Application of sequential extraction experiments to some sediments collected from gully pot in Seoul illustrates a much stronger scavenging effect by Fe and Mn-hydroxides, carbonates and organic phases. As deduced from both sequential extraction and leaching experiments, the relative mobility of heavy metals is found to be: Mn>Zn>>Co>Cd>>Cu>Pb>Cr>Fe, in spite of large differences in heavy metal content and localization. Changes in the physicochemical environments (such as acidification) caused by a traffic accident may result in the severe environmental pollution of heavy metals of surrounding area( surface water and rivers).

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A Mobile Robot Based on Slip Compensating Algorithm for Cleaning of Stud Holes at Reactor Vessel in NPP

  • Kim, Dong Il;Moon, Young Jun
    • 한국압력기기공학회 논문집
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    • 제16권1호
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    • pp.84-91
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    • 2020
  • The APR1400 reactor stud holes can be stuck due to high temperatures, high pressure, prolonged engagement, and load changes according to pressure changes in the reactor. Threaded surfaces of a stud hole should be cleaned for the sealing of pressure in reactor vessel by removing any foreign materials which may exist in the stud holes. Human workers can access to the stud hole for the cleaning of stud holes manually, but the radiation exposure of human workers is increased. Robot is an effective way to work in hazardous area. So we introduced robot for the cleaning of stud holes. Localization of mobile robots is generally based on odometry, but with increased mileage, position errors can be accumulated. In order to eliminate cumulative error and to ensure stability of its driving, laser sensors and new control algorithm were utilized. The distance between the robot and the wall was measured by laser sensors, and the control algorithm was implemented so as to travel the desired trajectory by using the measured values from sensors. The performance of driving and hole sensing were verified through field application, and mobile robot was confirmed to be applicable to the APR 1400 NPP.

Treatment of Bone Repair by Inductively Magnetic Fields

  • 안재목;;김희찬;민병구
    • 대한의용생체공학회:학술대회논문집
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    • 대한의용생체공학회 1992년도 춘계학술대회
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    • pp.213-217
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    • 1992
  • An inductively coupled magnetical signal (pulse wave, 0.7 to 60Hz, eighteen volts peak to Peak) that was applied non-invasively on the skin surface overlying the approximate site(measure position). In the group with unipolar pulse signal currents produced smaller than in the group with bipolar pulse signal. The signal was transmitted to the active coil, including a time-varying magnetic field: this in turn induced a the-varying electrical field in the field in the bone. It is very important to determine system parameters due to treatment time(healing) and the simplicity. This paper investigation was designed to compare the relative effects of pulsed unipolar currents with the effects of an identical pulsed bipolar currents. Since Inductive coupling is non-invasive and involves portable equipment, it is easy to apply and requires precise localization, it has distinct advantages and field characteristics along the bone for each different signal.

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FRP 어선 2종 스테인리스강 축의 음극방식을 위한 실용설계 및 적용방안 연구 (A Study on the Practical Cathodic Protection Design for the FRP Fishing Boat and It’s Application Scheme)

  • 강대선;김기준;이명훈;박정대;김태언
    • 선박안전
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    • 통권21호
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    • pp.66-77
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    • 2006
  • Stainless steel has been stably used closed by passivity oxidation films(Cr₂O₃) is made by neutral atmospheric environment. However, passivity oxidaton films of the surface of stainless steel occasionally comes to be destroyed in seawater which is influenced by an environment having galogen ion like Cl‾, then, localization corrosion comes to occur Stainless steel 304 for shaft system material of the small-size FRP fishing boat on seawater environments made an experiment on simulation of sacrifical anode(Al, Zn). Through these experiment and study, following results have been obtained ; According to the field inspection and corrosion simulation, the corrosion on the 2nd class stainless steel shaft(STS304) in FRP fishing boat has been verified to occur by crevice corrosion and galvanic corrosion etc., According to the comparison and analysis of Stainless steel 304 was severely corroded, but, protected shaft specimen was not totallay corroded. This result is assumed to be made by the facts that anodic reaction, Fe → fe²++ 2e¯, has been restricted by the cathodic protection current of sacrificial anode material.

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소형선박용 스테인리스강 축의 음극방식 응용 (Optimum Cathodic Protection for Stainless Steel Shaft of Small-Size Boat)

  • 배일용;박정대;강대선;이명훈;김기준
    • 한국마린엔지니어링학회:학술대회논문집
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    • 한국마린엔지니어링학회 2005년도 후기학술대회논문집
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    • pp.232-233
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    • 2005
  • Stainless steel has been stably used closed by passivity oxidation films($Cr_2O_3$) is made by neutral atmospheric environment. However, passivity oxidation films of the surface of stainless steel occasionally comes to be destroyed in seawater which is influenced by an environment having halogen ion like $Cl^-$, then, localization corrosion comes to occur. Stainless steel 304 for shaft system material of the small-size FRP fishing boat on seawater environments made an experiment on simulation of sacrifical anode(Al, Zn). Through these experiment and study, following results have been obtained ; According to the field inspection and corrosion simulation, the corrosion on the 2nd class stainless steel shaft(STS 304) in FRP fishing boat has been verified to occur by crevice corrosion and galvanic corrosion etc.. According to the comparison and analysis of Stainless steel 304 shaft materials after simulation leaving unprotected and applying cathodic protection, unprotected shaft specimen of stainless steel 304 was severely corroded, but, protected shaft specimen was not totally corroded. This result is assumed to be made by the facts that anodic reaction, $Fe{\rightarrow}Fe^{2+}$ + $2e^-$, has been restricted by the cathodic protection current of sacrificial anode material.

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Localization (and profiles) of tyrosine-phosphorylated proteins in female reproductive organs of adult rats

  • Bunsueb, Sudtida;Tangsrisakda, Nareelak;Wu, Alexander T.H.;Iamsaard, Sitthichai
    • Clinical and Experimental Reproductive Medicine
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    • 제47권3호
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    • pp.180-185
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    • 2020
  • Objective: Tyrosine phosphorylation is an essential process in many biological systems, including the male reproductive system. The presence of tyrosine-phosphorylated (TyrPho) proteins has been well documented in male reproductive organs, but research in fertile females is still limited. Methods: The ovary, oviduct, and uterus of adult female Sprague-Dawley rats in the estrus phase were used to localize TyrPho proteins using an immunohistochemical technique. These proteins were separated and their expression patterns were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis, respectively. Results: TyrPho proteins were localized in the cytoplasm of the oocyte except the antral fluid; in the granulosa cells, theca cells, and stromal cells of the ovary; at the apical surface of oviductal epithelial cells; and in the basal epithelium and submucosa of the uterine wall. Moreover, we found that 72-, 43-, and 28-kDa TyrPho proteins were localized in the ovary, while 170-, 55-, and 43-kDa proteins were localized in the oviduct. In the uterus, we detected four major bands, corresponding to 61-, 55-, 54-, and 43-kDa TyrPho proteins. Conclusion: Given that these TyrPho proteins were found in major reproductive organs in the estrus phase, these proteins may play important roles in female fertility.

텅스텐 미세 전극을 이용한 전해 가공 (Electrochemical Machining Using Tungsten Microelectrode)

  • 류시형;유종선
    • 한국정밀공학회지
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    • 제26권4호
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    • pp.134-140
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    • 2009
  • The feasibility of electrochemical drilling and milling on stainless steel are investigated using tungsten microelectrode with $10{\mu}m$ in diameter. For the development of environmentally friendly and safe electrochemical process, citric acid solution is used as electrolyte. A few hundred nanoseconds duration pulses are applied between the microelectrode and work material for dissolution localization. Tool fracture by Joule heating, micro welding, capillary phenomenon, tool wandering by the generated bubbles are observed and their effects on micro ECM are discussed. Occasionally, complex textures including micro pitting corrosion marks appeared on the hole inner surface. Metal growth is also observed under the weak electric conditions and it hinders further dissolutions for workpiece penetration. By adjusting appropriate pulse and chemical conditions, micro holes of $37{\mu}m$ in diameter with $100{\mu}m$ in depth and 26Jim in diameter with $50{\mu}m$ in depth are drilled on stainless steel 304. Also, micro grooves with $18{\mu}m$ width and complex micro hand pattern are machined by electrochemical milling.