Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
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Localization (and profiles) of tyrosine-phosphorylated proteins in female reproductive organs of adult rats

  • Bunsueb, Sudtida (Department of Anatomy, Faculty of Medicine, Khon Kaen University) ;
  • Tangsrisakda, Nareelak (Department of Anatomy, Faculty of Medicine, Khon Kaen University) ;
  • Wu, Alexander T.H. (College of Medical Science and Technology, Taipei Medical University and Academia Sinica) ;
  • Iamsaard, Sitthichai (Department of Anatomy, Faculty of Medicine, Khon Kaen University)
  • Received : 2020.02.05
  • Accepted : 2020.04.01
  • Published : 2020.09.30
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Abstract

Objective: Tyrosine phosphorylation is an essential process in many biological systems, including the male reproductive system. The presence of tyrosine-phosphorylated (TyrPho) proteins has been well documented in male reproductive organs, but research in fertile females is still limited. Methods: The ovary, oviduct, and uterus of adult female Sprague-Dawley rats in the estrus phase were used to localize TyrPho proteins using an immunohistochemical technique. These proteins were separated and their expression patterns were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis, respectively. Results: TyrPho proteins were localized in the cytoplasm of the oocyte except the antral fluid; in the granulosa cells, theca cells, and stromal cells of the ovary; at the apical surface of oviductal epithelial cells; and in the basal epithelium and submucosa of the uterine wall. Moreover, we found that 72-, 43-, and 28-kDa TyrPho proteins were localized in the ovary, while 170-, 55-, and 43-kDa proteins were localized in the oviduct. In the uterus, we detected four major bands, corresponding to 61-, 55-, 54-, and 43-kDa TyrPho proteins. Conclusion: Given that these TyrPho proteins were found in major reproductive organs in the estrus phase, these proteins may play important roles in female fertility.

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References

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