• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• 제26권5호
• /
• pp.481-489
• /
• 2000

This research was focused on overall examination of tissue alteration, wound healing promotion. After the hair on the dorsal surface was shaved, $5{\times}5mm$ oval skin defect was formed. Experimental wounds of right side were irradiated on every day for 90 second with Ga-Al-As semi-conductor laser. Left side wounds served as control group. The rats were sacrificed on the 1st, 3rd, 5th, 7th, 14th, 21th day. For light microscopically, parafin section were stained with H&E, MT. The outcomes were as follows : 1. On 1st day, experimental and control group were seen acute inflammatory cell infiltration, edema. 2. On the 3rd days, both groups were seen crust development, collagen, blood vessel proliferation. 3. On the 5th days, experimental group were reduced edema and inflammatory cell infiltration than control group. 4. On the 7th days, both groups were observed edema, inflammatory cell infiltration disappearance and keratinocytes motility from wound defect. 5. On the 14th days, experimental group appeared collagen, blood vessel proliferation and hair follicle than control group. 6. On the 21th days, both groups were seen normal status re-epithelization. According to the above results, The wound-healing stimulated by laser radiation involves an increased rate of epithelial growth. LLLT was confirmed that it has fibroblast, blood vessel proliferation, influence initial wound healing process.

• Asian Pacific Journal of Cancer Prevention
• /
• 제16권17호
• /
• pp.7497-7500
• /
• 2015

Background: Tumor markers, designated as a broad group of substances produced by malignancies, could be in the form of biochemical substances, immunological substances, cell surface changes and genetic alterations. Cancer, a disorder of cellular behavior is characterized by alteration of serum glycoproteins. L-fucose, a hexose, which is the terminal sugar in most of the plasma glycoproteins, may be useful as a tumor marker for the detection, monitoring and prognostic assessment of malignancies. The aim of the study was to ascertain the role of serum fucose as a biomarker for early detection of oral cancer and to compare serum fucose levels in healthy controls, leukoplakia and oral cancer patients. Materials and Methods: The study included 60 (100.0%) subjects, who were grouped as 20 (33.3%) control subjects, 20 (33.3%) squamous cell carcinoma patients and 20 (33.3%) leukoplakia patients. Fucose estimation was done using UV-visible spectrophotometry based on the method as adopted by Winzler using cysteine reagent. The results were analyzed statistically using ANOVA with Bonferroni post hoc tests. Results: Results showed a high significance in serum fucose in oral squamous cell carcinoma (OSCC) and leukoplakia subjects compared to normal controls. There was a gradual increase in the values noted from control to leukoplakia and to squamous cell carcinoma. Conclusions: Estimation of serum fucose may be a reliable marker and can be used as an effective diagnostic biomarker in oral squamous cell carcinoma patients.

• 대한소아치과학회지
• /
• 제41권4호
• /
• pp.322-327
• /
• 2014

국소적 치아이형성증(Regional Odontodysplasia)은 드물게 발생하는 발육성 장애로, 이환된 치아는 임상적, 방사선학적, 조직학적으로 특징적인 소견을 보인다. 국소적 치아이형성증은 유치열과 영구치열에 모두 영향을 미치며, 임상적으로 정상치아보다 크기가 작거나, 거칠고 불규칙한 치면을 가지는 등 형성부전, 석회화부전의 양상을 보인다. 방사선학적으로는 법랑질과 상아질이 얇고, 경계가 뚜렷하지 않아 방사선불투과성이 명확하지 않으며, "ghost-like appearance"와 같은 특징적인 소견을 보인다. 조직학적으로는 저석회화된 법랑질이 보이며, 법랑소주가 불규칙한 방향을 나타내고, 상아세관의 수가 감소되어 있다. 국소적 치아이형성증의 원인은 정확히 밝혀지지 않았으며, 상악 좌측에서 호발하고, 인종간의 유병률 차이는 존재하지 않으며, 남성에 비해 여성에서 더 호발하는 것으로 알려져 있다. 이 증례는 4세 6개월의 환아에서 임상적, 방사선학적 검사 결과 상악 우측 악궁에 발생한 국소적 치아이형성증으로 진단하고, 5년간의 주기적인 관찰을 시행하였다. 국소적 치아이형성증에 이환된 치아는 취약한 치면과 미성숙한 치근 때문에 예후가 불량할 수 있으므로 개개치아에 대한 주의깊은 임상적, 방사선학적 관찰을 통한 시기적절한 치료계획 수립이 중요할 것이다.

• 분석과학
• /
• 제5권3호
• /
• pp.277-283
• /
• 1992

질산, 염산, 황산 그리고 질산과 염산의 1:1 혼산의 농도를 변화시키면서 유도결합 플라즈마 원자방출분광법(ICP-AES)의 신호크기에 미치는 산의 영향을 연구하였다. 거의 모든 측정원소의 신호크기가 감소되었다. 산의 존재로 인한 감소는 산 농도가 1% 이상 될 때 심하게 눈에 띄었고, 사용된 산 중에서 황산에 의한 감소가 가장 심했으며 그 정도를 예측하기 힘들었다. 산의 농도를 변화시키면서 측정 원소의 신호크기 대 Ar 신호크기의 비, 그리고 Mg II 신호크기 대 Ma I 신호의 비를 측정하였다. 이 연구에서, 신호크기 감소의 주된 원인은 플라즈마 들뜸특성의 변화에 의한 것이 아니고, 분무효율의 변화, 예를 들면 droplet 크기분포, 점도와 표면장략의 변화 등인 것으로 판명되었다. 유도결합 플라즈마 질량분석법(ICP-MS)에서는 신호크기의 감소와 이온화 에너지와의 상관관계는 발견되지 않았다.

• 한국임상수의학회지
• /
• 제30권4호
• /
• pp.283-287
• /
• 2013

Canine skin is a highly dynamic organ that is constantly adapted to changes in its environment. It provides structural, sensory, immunologic, and physiologic functions and contributes an essential barrier function against potential environmental insults. We assessed the changes of canine skin hydration, pH, and protein contents by noninvasive method according to the frequency of application with canine commercial shampoo. Canine commercial shampoo was applied topically every other day, 5 days, and 8 days on different sites, respectively in 8 dogs. Saline-applied site was as a control. Skin hydration was increased and skin surface pH was decreased significantly in canine commercial shampoo-applied site every other day (p < 0.05). Also, skin protein concentration measured by D-squame$^{(R)}$ tape stripping method was increased significantly in canine commercial shampoo-applied site every other day (p < 0.05). There were alterations on every 5 days and 8 days, but not significantly. These results may be recognized that the frequent use of canine commercial shampoo cause abnormalities of the skin barrier function and alteration of stratum corneum integrity/cohesion. Consequently it was suggested that bathing the dog with canine commercial shampoo was appropriate every 5 or 8 days.

• The Plant Pathology Journal
• /
• 제30권3호
• /
• pp.245-253
• /
• 2014

Antimicrobial peptides (AMPs) are small but effective cationic peptides with variable length. In previous study, four hexapeptides were identified that showed antimicrobial activities against various phytopathogenic bacteria. KCM21, the most effective antimicrobial peptide, was selected for further analysis to understand its modes of action by monitoring inhibitory effects of various cations, time-dependent antimicrobial kinetics, and observing cell disruption by electron microscopy. The effects of KCM21 on Gram-negative strain, Pseudomonas syringae pv. tomato DC3000 and Gram-positive strain, Clavibacter michiganensis subsp. michiganensis were compared. Treatment with divalent cations such as $Ca^{2+}$ and $Mg^{2+}$ inhibited the bactericidal activities of KCM21 significantly against P. syringae pv. tomato DC3000. The bactericidal kinetic study showed that KCM21 killed both bacteria rapidly and the process was faster against C. michiganensis subsp. michiganensis. The electron microscopic analysis revealed that KCM21 induced the formation of micelles and blebs on the surface of P. syringae pv. tomato DC3000 cells, while it caused cell rupture against C. michiganensis subsp. michiganensis cells. The outer membrane alteration and higher sensitivity to $Ca^{2+}$ suggest that KCM21 interact with the outer membrane of P. syringae pv. tomato DC3000 cells during the process of killing, but not with C. michiganensis subsp. michiganensis cells that lack outer membrane. Considering that both strains had similar sensitivity to KCM21 in LB medium, outer membrane could not be the main target of KCM21, instead common compartments such as cytoplasmic membrane or internal macromolecules might be a possible target(s) of KCM21.

• 한국물환경학회지
• /
• 제26권1호
• /
• pp.61-70
• /
• 2010

The increase of impervious cover (IC) in a watershed is known as an important factor causing alteration of water cycle, deterioration of water quality and biological communities of urban streams. The study objective was to assess the impact of IC changes on the surface runoff characteristics of Kap Stream basin located in Geum river basin (Korea) using the Storm Water Management Model (SWMM). SWMM was calibrated and verified using the flow data observed at outlet of the watershed with 8 days interval in 2007 and 2008. According to the analysis of Landsat satellite imagery data every 5 years from 1975 to 2000, the IC of the watershed has linearly increased from 4.9% to 10.5% during last 25 years. The validated model was applied to simulate the runoff flow rates from the watershed with different IC rates every five years using the climate forcing data of 2007 and 2008. The simulation results indicated that the increase of IC area in the watershed has resulted in the increase of peak runoff and reduction of travel time during flood events. The flood flow ($Q_{95}$) and normal flow ($Q_{180}$) rates of Kap Stream increased with the IC rate. However, the low flow ($Q_{275}$) and drought flow ($Q_{355}$) rates showed no significant difference. Thus the subsurface flow simulation algorithm of the model needs to be revisited for better assessment of the impact of impervious cover on the long-term runoff process.

• 한국식품과학회지
• /
• 제46권6호
• /
• pp.778-781
• /
• 2014

비가열 살균 기술의 하나인 광펄스 기술을 이용하여 마른 김에 존재하는 미생물에 대한 살균 효과와 품질 변화에 대하여 알아보았다. 마른 김에 1,000 V, 5 pps의 조건하에서 7.9 cm의 거리에서 광펄스 조사를 하였을 때 처리 1분 후에는 0.6 log CFU/g, 처리 10분 후에는 1.6 log CFU/g의 사멸 효과를 보였다. 같은 조건에서 광펄스 조사 후 마른 김의 표면 온도는 약 $1.9^{\circ}C$의 상승을 나타냈으며, 색도에 있어서는 명도가 증가하고, 적색도 및 황색도는 감소하는 경향을 보였으며, 처리 시간이 길어질수록 전체적인 색도의 차이가 크게 나타나는 결과를 보였으나 육안으로는 차이 정도를 확인할 수 없었다. 이러한 결과로 미루어 볼 때 광펄스 살균 기술은 품질 변화없이 마른 김에 존재하는 위해 미생물을 효과적으로 감소시킬 수 있을 것으로 판단된다.

• 한국태양에너지학회 논문집
• /
• 제28권1호
• /
• pp.9-17
• /
• 2008

An experimental investigation has been carried out to study the effects of different working fluids on the behavior and thermal performance of a hi-directional thermodiode. The thermodiode was made up of two rectangular loops mounted between a collector plate and a radiator plate. Rotatable joints between the horizontal and inclined segments of the loops enable easy alteration of the direction of heat transfer. The loops and the tank were filled with a working fluid for effective heat transfer when the thermodiode was forwarded biased. Six different working fluids were tested with thermal conductivity values ranging from 0.1 to $0.56W/m-^{\circ}C$, thermal expansion coefficient values ranging from $1.8\;{\times}10^{-4}$ to $1.3\;{\times}\;10^{-3}\;K^{-1}$, and kinematic viscosity values ranging from $0.65\;{\times}\;10^{-6}$ to $100\;{\times}\;10^{-6}\;m^2/s$. Especially, mixtures of $Al_2O_3$ (30nm Particle) in deionized water have been tested for the volumetric ratios of 0.01, 0.02, 0.03, 0.1, 0.2%. Each experiment was carried out after the loop was filled with a working fluid for effective heat transfer and the thermodiode was forwarded biased. The solar thermodiode was heated by a radiant heater consisting of 20 halogen lamps that generated a heat flux of about $1000\;W/m^2$ on the collector surface. Results are given in terms of temperature development in different parts of the loop as heat is delivered from its hot end to the surrounding atmosphere by the radiator made of copper plates.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• 제21권2호
• /
• pp.120-130
• /
• 1999

This investigation studied the effect of ovariectomy on the temporomandibular joint (TMJ) of rat. Forty female Sprauge-Dawley strain white rats, each weighing about 200grams were used and divided into two groups. The one group was performed ovariectomy, and the other was performed sham operation. Both group were killed at the intervals of 3 days, 1, 2, 4, and 8 weeks postoperatively, paraffin section were stained with H&E, MT and histomorphometric study of the TMJ was performed in a synchronous manner with an sham operated control group The result were as follows: 1. At 4 weeks after ovariectomy, an adhesion between the inferior surface of the articular disc and the condyle was observed in the central portion. At 8 weeks after ovariectomy, a focal thickening of the bone (osteophyte) was observed in anterior portion and there was a marked decrease in the trabecular bone in the posterior region. 2. The thickness of cartilage zone was increased at 2 weeks postopratively in the central portion, and decreased at 4 weeks postopratively in the posterior portion. The thickness of transitional zone was decreased at 1 week postoperatively in the anterior and proliferating zone was decreased postoperatively. The thickness of articular zone was decreased at 3 days postopratively in the anterior portion, and decreased at 1 week postoperatively in the central portion. From the above finding, we could conclue that estrogen deficiency in rats causes alteration of TMJ in marked early stage.