• 대한본초학회지
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• 제21권4호
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• pp.103-107
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• 2006

Objectives : This study was investigated to analyze the anti-oxidant activity of water and ethanol extracts from the Magnoliae Flos. Methods : The effects on electron donating ability (EDA), SOD-like activity and xanthine oxidase inhibition have been investigated the anti-oxidant activity for utilization as a natural material of cosmetics. Results : This study was investigated to analyze the anti-oxidant activity of water and ethanol extracts from the Magnoliae Flos. In the electron donating ability test, water and ethanol extract from Magnoliae Flos showed an effect of 75.6% and 83.5% at 1,000 ppm concentration, respectively. In the superoxide dismutase (SOD)-like activity, 1,000 ppm of water extract showed an effect of 45.2% and ethanol extract showed an effect of 64.2%. Xanthine oxidase showed a meaningful inhibitory effect of 68.8% in water extract and 23.2% at 1,000 ppm in ethanol extract. Conclusion : From these results, Magnoliae Flos showed the development possibility as a natural material of anti-oxidant cosmetics.

• 셀메드
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• 제13권2호
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• pp.2.1-2.9
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• 2023

CPS219, a new concept compound composed of coffee (CO), Pueraria Tomsonii flower (PF), and Sambou bamboo salt^TM (BS), was developed as a coffee beverage to alleviate menopausal symptoms. The purpose of this study is to evaluate the anti-oxidant and menopausal alleviating effects of CPS219 combined as the optimal ratio of each component selected through sensory evaluation and blind consumer test. For CPS219, the optimal ratio of CO, PF, and BS was determined to be 1:0.1:0.017 through various sensory evaluations and blind consumer tests. CPS219 significantly enhanced the superoxide dismutase-like activity compared to the CO or CO plus PF (CP). The proliferation of MCF-7 cells was considerably increased after 24 hours by treatment with CO, CP, or CPS219, but only CPS219 significantly boosted the proliferation of MCF-7 cells after 48 hours. Moreover, CPS219 had an estrogen-like effect by dramatically increasing the expression of estrogen receptor-β mRNA in MCF-7 cells but not CO and CP. Treatment of MCF-7 cells with CO, CP, or CPS219 did not cause any cytotoxicity. In conclusion, these findings imply that anti-oxidant and estrogen-like properties of CPS219 can be used to prevent and cure postmenopausal symptoms.

• 한국환경과학회지
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• 제17권10호
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• pp.1139-1146
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• 2008

To investigate the functional properties and the antioxidant effect of pine needle(Pinus densiflora), the pine needle extract was obtained with methanol and its functionality was measured by spectrophotometric method, and the antioxidant experiment on soybean oil was carried out by the active oxygen method. The extraction yield of pine needle with 99% methanol was about 19%, the total flavonoid content of the pine needle-methanol extract was 11.32 mg/g on dry basis and the superoxide dismutase-like activity was 94.3%. The nitrite scavenging ability of the extract was pH dependent with the values of 77.44% at pH 1.2, 48.45% at pH 3.0 and 11.04% at pH 6.0, respectively. The peroxide value was 92.6 meq/kg at 5% dosage, 138.4 meq/kg at 2% dosage of the extract on 8 oxidation days. The period of the peroxide value to be 100 mg/kg was 4.9, 6.3 and 8.5 days at control, 2% and 5% dosage of extract, respectively. And the relative antioxidant effectiveness of the extract was 27.9% and 72.3% increase at 2% and 5% dosage, respectively, compared to control. The thiobarbituric acid value showed few differences within 4 oxidation days, but with the dosage of the extract it fairly decreased with considerable antioxidant effect to control above 4 days.

• 한국식품저장유통학회지
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• 제13권6호
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• pp.732-739
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• 2006

볶음처리한 새송이버섯의 마이크로웨이브 추출조건, 즉 에탄올 농도, 추출시간 및 마이크로웨이브 power에 따른 품질특성을 살펴보았다. 총 페놀성 화합물 함량, 전자공여능 및 pH 1.2에서의 아질산염소거능은 50% 에탄올로 추출한 경우 높게 나타났다. SOD 유사활성은 75% 에탄을 농도로 추출하였을때 가장 높은 활성을 나타났다. 가용성 고형분 및 총 페놀성 화합물 함량은 추출시간이 증가할수록 증가하다가 7분일 때 가장 높게 나타났으며, 전자공여능은 추출시간이 증가할수록 감소하는 것으로 나타났으며, SOD유사활성 및 아질산염소거능의 경우추출시간에 따른 유의적인 차이가 없는 것으로 나타났다. 마이크로웨이브 power 의 경우 100 W로 추출하였을 때 가용성 고형분, 총 페놀성화합물 함량 및 전자공여능 가장 높게 나타났다. SOD 유사 활성은 25 W일 때 높은 활성을 나타내었다. 그러나 아질산염 소거능은 마이크로웨이브 power에 영향을 많이 받지 않는 것으로 나타났다.

• Preventive Nutrition and Food Science
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• 제9권3호
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• pp.283-288
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• 2004

Superoxide dismutase (SOD) from tomato (Lycopersicon esculentum Mill.) fruit was purified by ammonium sulphate precipitation, Sephadex G-100 and DEAE-cellulose column chromatographies. A 22 fold purification and an overall yield of 44% were achieved. The purified enzyme was a homodimer with Mr 37.1 kDa and subunit Mr 18.2 kDa as judged by SDS-PAGE. SOD showed $K_{m}$ values of 25 ${\times}$ 10$^{-6}$ M and 1.7 ${\times}$ 10$^{-6}$ M for nitroblue tetrazolium (NBT) and riboflavin as substrates, respectively. The enzyme was thermostable upto 5$0^{\circ}C$ and exhibited pH optima of 7.8. The effect of metal ions and some other compounds on enzyme activity was studied. $Co^{2+}$ and $Mg^{2+}$ were found to enhance relative enzyme activities by 27 % and 73 %, respectively, while M $n^{2+}$ inhibited the SOD activity by 64%. However, $Ca^{2+}$ and C $u^{2+}$ had no effect on enzyme activity. Other compounds like $H_2O$$_2$ and Na $N_3$ inhibited enzymatic activities by 60% and 32%, respectively, while sodium dodecyl sulphate (SDS), chloroform plus ethanol and $\beta$-mercaptoethanol had no effect on the activity of SOD. of SOD.

• 한국식품저장유통학회지
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• 제13권5호
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• pp.643-648
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• 2006

본 연구는 뜰보리수 열매의 식품영양학적 평가 및 기능성 물질 발굴의 일환으로 뜰보리수 열매를 이용하여 성숙과정 중의 free radical 소거능을 비롯한 SOD유사활성, 아질산염 소거작용, xanthine oxidase 저해활성 등의 항산화 활성을 조사하였다. 뜰보리수 에탄올 추출물의 전자공여능은 성숙함에 따라 감소하는 경향을 나타내었으며, 에탄올 추출물의 농도가 높을수록 전자공여능의 효과는 더욱 높게 나타났다. 에탄올 추출물의 SOD 유사활성은 성숙정도에 따른 증가하였고, 농도에 비례하였다. 뜰보리수 열매의 에탄을 추출물의 아질산염 소거능은 pH가 낮을수록 높았으며, 농도가 증가할수록 아질산염 소거 작용이 높게 나타났다. Xanthine oxidase 활성 저해능은 완숙과 과숙과실의 추출물에 비해서 미숙과실 추출물에서 높았다.

• 셀메드
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• 제6권4호
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• pp.28.1-28.6
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• 2016

Oxidative rancidity in foods causes undesirable changes in nutritive value, aroma, flavor, and color. Salix Koreensis Andersson in DC. (SK) has anti-oxidative and anti-inflammatory effects and is traditionally used to treat neuralgia, edema, pain, and inflammatory diseases. However, the regulatory effects of SK on oxidative and inflammatory reactions have not been elucidated. In this context, we scientifically validated the anti-oxidative and anti-inflammatory activities of SK leaf (SKL). The methanol extract of SKL was evaluated for in vitro anti-oxidative activities. SKL showed increased superoxide dismutase (SOD)-like activity and 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity. The in vitro anti-oxidant and anti-inflammatory activities of SKL were also investigated in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages. LPS resulted in decreased SOD activities compared with the unstimulated cells, but SKL significantly increased SOD activities reduced by LPS. In addition, LPS-induced nitric oxide, tumor necrosis factor-${\alpha}$, and interleukin-6 productions were significantly and dose-dependently reduced by SKL in RAW264.7 macrophages without inducing cytotoxicity. In conclusion, these results indicate that SKL will be able to be effectively used as a food additive with anti-oxidative and anti-inflammatory effects.

• 한국식품영양학회지
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• 제21권2호
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• pp.157-164
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• 2008

This study examined the antioxidant activity of methanol and water extracts from Orostachys japonicus A. Berger according to harvest times, by measuring electron donating ability(EDA), reducing power, superoxide dismutase(SOD)-like activity, thiobarbituric acid reactive substances(TBARS) and antioxidant activity within a linoleic acid emulsion. EDA increased proportionally with the extract concentration and the methanol extract had slightly stronger effects than the water extract. And reducing power and SOD-like activity were highest in the methanol extract. Overall, based on the data, the methanol extract of O. japonicus A. Berger harvested during $August{\sim}October$ presented the highest level of antioxidative activity and may be a good candidate as a natural antioxidant source.

• 대한약침학회지
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• 제13권3호
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• pp.5-13
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• 2010

Reactive Oxygen Species(ROS) are continuously produced at a high rate as a by-product of aerobic metabolism. Since tissue damage by free radical increases with age, the reactive oxygen species(ROS) such as hydrogen peroxide($H_2O_2$), nitric oxide(NO). Several lines of evidence provided that ROS appears to cause to develop aging-related various diseases such as cancer, arthritis, cardiovascular disease. Our reserch objective was to examine the in vitro biological activity of Scolopendrid Pharmacopuncture, including the total poly-phenol content, DPPH radical scavenging, ABTS radical scavenging, Superoxide dismutase(SOD)-like activity, Nitrite scavenging ability. The total poly-phenol contents of Scolopendrid Pharmacopuncture was 35.859mg/L. Elctron donation ability on DPPH was 36.82%. The 2,2'-azinobis-3-ehtlbezothiazoline-6-sulfonic acid radical decolorization (ABTS) was 84.7%. The superoxide dismutase (SOD)-like activities of Scolopendrid Pharmacopuncture was 44.33%. The nitrite scavenging effects were pH dependent, and were highest at pH 1.5(45.2%) and lowest at pH 6.0(11.3%). We conclude that Scolopendrid Pharmacopuncture may be useful as potential sources of antioxidant.

• 한국식품영양학회지
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• 제24권3호
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• pp.306-311
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• 2011

본 연구에서는 쇠비름 추출물의 항산화 효과 및 위궤양 유발 병원균으로 잘 알려진 Helicobacter pylori에 대한 항균 효과를 알아보고자 하였다. 쇠비름의 유기용매 추출물의 항산화 효과는 총 페놀 함량, 전자공여능, SOD 유사활성, TBARS 등을 통하여 검증하였다. 쇠비름의 총 페놀 함량은 약탕기 추출물에서 72.2 mg%로 가장 높게 나타났고, 전자공여능은 ethyl acetate 분획물이 91.8%와 methanol 분획물이 90.6%로 나타났다. SOD 유사활성은 2.5 mg/$m{\ell}$의 농도에서 ethyl acetate 분획물 17.4%와 petroleum ether 분획물 16.4%로 높게 나타났다. 쇠비름의 TBARS에 의한 항산화 효과는 유의적으로 나타나지 않았다. Paper disc 법에 의한 Helicobacter pylori 항균 활성의 경우, 쇠비름 추출물의 농도가 증가할수록 inhibition zone 크기가 증가하였고, 쇠비름의 petroleum ether 분획물 10,000 ppm 농도에서 가장 큰 활성이 나타났다.