The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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The Antioxidant Effect of Portulaca oleracea Extracts and Its Antimicrobial Activity on Helicobacter pylori

쇠비름 추출물의 항산화 효과 및 Helicobacterpylori에 대한 항균 활성

  • Park, So-Hae (Dept. of Food Science and Nutrition, Keimyung University) ;
  • Kim, Dae-Kwang (Dept. of Anatomy, School of Medicine, Keimyung University) ;
  • Bae, Ji-Hyun (Dept. of Food Science and Nutrition, Keimyung University)
  • 박소해 (계명대학교 식품영양학과) ;
  • 김대광 (계명대학교 의과대학 해부학교실) ;
  • 배지현 (계명대학교 식품영양학과)
  • Received : 2011.04.21
  • Accepted : 2011.07.26
  • Published : 2011.09.30
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Abstract

This study was performed to investigate the effect of Portulaca oleracea extract on the antioxidant and antimicrobial activities against Helicobacter pylori. The each solvent extracts prepared from Portulaca oleracea were investigated by measuring total phenolic compounds, electron donating ability, superoxide dismutase-like ability and thiobarbituric acid reactive substances The herb extractor extract yielded the highest content of total phenolic compounds(72.2 mg%). The electron donating abilities(EDA) of ethyl acetate and methanol extracts showed high antioxidant activity. The superoxide dismutase (SOD)-like abilities of ethyl acetate and petroleum ether extracts also showed some activity. The antioxidant activity of thiobarbituric acid reactive substances was not significant. The petroleum ether extract of Portulaca oleracea showed the highest antimicrobial activity at 10,000 ppm concentration.

본 연구에서는 쇠비름 추출물의 항산화 효과 및 위궤양 유발 병원균으로 잘 알려진 Helicobacter pylori에 대한 항균 효과를 알아보고자 하였다. 쇠비름의 유기용매 추출물의 항산화 효과는 총 페놀 함량, 전자공여능, SOD 유사활성, TBARS 등을 통하여 검증하였다. 쇠비름의 총 페놀 함량은 약탕기 추출물에서 72.2 mg%로 가장 높게 나타났고, 전자공여능은 ethyl acetate 분획물이 91.8%와 methanol 분획물이 90.6%로 나타났다. SOD 유사활성은 2.5 mg/$m{\ell}$의 농도에서 ethyl acetate 분획물 17.4%와 petroleum ether 분획물 16.4%로 높게 나타났다. 쇠비름의 TBARS에 의한 항산화 효과는 유의적으로 나타나지 않았다. Paper disc 법에 의한 Helicobacter pylori 항균 활성의 경우, 쇠비름 추출물의 농도가 증가할수록 inhibition zone 크기가 증가하였고, 쇠비름의 petroleum ether 분획물 10,000 ppm 농도에서 가장 큰 활성이 나타났다.

Keywords

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