• Korean Journal of Pharmacognosy
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• v.6 no.4
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• pp.211-217
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• 1975

The blood pressure response to Junci Herba water and methanol extracts in rabbit and $LD_{50}$ to Junci Herba water extract in mouse were investigated in order to develop a hypotensive agent from natural resources. $LD_{50}$ of Junci Herba water extract (WE) was 600 mg/kg in mouse, when WE was injected intraperitonealy. Junci Herba water and methanol extract, when injected into the vein of rabbit, produced a fall of blood pressure. Hypotensive effect of WE was suppressed by atropine and potentiated by phentolamine, while not affected by avil, propranolol, and phenoxybenzamine. Intravenous injection of chlorisondamine weakened the hypotensive effect of WE, and WE produced hypertensive effect in this rabbit. Intravenous injection of bretylium did not affect the hypotensive effect of WE, but WE produced hypertensive effect in this rabbit. In the rabbit treated with chlorisondamine, hypertensive effect of WE was suppressed by methysergide or bretylium, but not affected by atropine or phenoxybenzamine.

• Journal of Microbiology and Biotechnology
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• v.16 no.12
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• pp.1928-1934
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• 2006

The rubradirin biosynthetic gene cluster harbors 58 ORFs within a 105.6-kb sequence, which includes all of the genes responsible for the synthesis of rubradirin, as well as the primary genes relevant to regulatory, resistance, and transport functions. This gene cluster also harbors a resistance-mediating ABC transporter, RubT1, which is located at the most upstream position in the cluster. In the present study, RubT1 was expressed heterologously in E. coli, and the resistance affinity of RubT1 was determined by an antibacterial activity test, as well as by HPLC and ESI-MS analyses. Evidence clearly demonstrates that RubTl mediates rubradirin resistance as an ABC transporter.

• Journal of Microbiology and Biotechnology
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• v.22 no.8
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• pp.1059-1065
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• 2012

Biocatalytic transfer of oxygen in isolated cytochrome P450 or whole microbial cells is an elegant and efficient way to achieve selective hydroxylation. Cytochrome P450 CYP105P2 was isolated from Streptomyces peucetius that showed a high degree of amino acid identity with hydroxylases. Previously performed homology modeling, and subsequent docking of the model with flavone, displayed a reasonable docked structure. Therefore, in this study, in a pursuit to hydroxylate the flavone ring, CYP105P2 was co-expressed in a two-vector system with putidaredoxin reductase (camA) and putidaredoxin (camB) from Pseudomonas putida for efficient electron transport. HPLC analysis of the isolated product, together with LC-MS analysis, showed a monohydroxylated flavone, which was further established by subsequent ESI/MS-MS. A successful 10.35% yield was achieved with the whole-cell bioconversion reaction in Escherichia coli. We verified that CYP105P2 is a potential bacterial hydroxylase.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.13 no.11
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• pp.5560-5579
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• 2019

Searchable symmetric encryption (SSE) scheme can perform search on encrypted data directly without revealing the plain data and keywords. At present, many constructive SSE schemes were proposed. However, they cannot really resist the malicious adversary, because it (i.e., the cloud server) may delete some important data. As a result, it is very likely that the returned search results are incorrect. In order to better guarantee the integrity of outsourcing data, and ensure the correction of returned search results at the same time, in this paper, we combine SSE with blockchain (BC), and propose a SSE-on-BC framework model. We then construct two concrete schemes based on the size of the data, which can better provide privacy protection and integrity verification for data. Lastly, we present their security and performance analyses, which show that they are secure and feasible.

• Journal of Korean Medical classics
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• v.20 no.2
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• pp.289-313
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• 2007

Many of newly emerging diseases such as hypertension, stroke, diabetes, obesity, and atoptic dermatities are categorized into "Fire heat-disease(火熱病)" according to oriental medicine. It is because factors such as stress, overeating, and high fat diet are major causes of heat in the human body. Sun Yi-Kui(孫一奎), a well-known oriental medical doctor during Ming Dynasty's "Jia Jing Wan Li Nian Jian"(嘉靖萬歷年間 : A.D $1522^{\sim}1619$), established new theories on "Fire heat-disease-mechanism(火熱病機)". This study aims to investigate Dr. Sun's "Fire heat-disease-mechanism(火熱病機)"theory. For this purpose, I thoroughly examined the concepts of "Dong-Qj(動氣)", "Ming-Men(命門)", king fire(君火) and minister fire(相火) as well as pathological theory on "Fire heat(火熱)". In addition, I compared Sun's theory with those of Li Dong-yuan and Zhu dan-Xi.

• Structural Engineering and Mechanics
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• v.62 no.6
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• pp.709-717
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• 2017

This paper develops a two-stage method for structural damage identification by using modal data. First, the Residual Force Vector (RFV) is introduced to detect any potentially damaged elements of structures. Second, data of the frequency domain are used to build up the objective function, and then the Imperialist Competitive Algorithm (ICA) is utilized to estimate damaged extents. ICA is a heuristic algorithm with simple structure, which is easy to be implemented and it is effective to deal with high-dimension nonlinear optimization problem. The advantages of this present method are: (1) Calculation complexity can be decreased greatly after eliminating many intact elements in the first step. (2) Robustness, ICA ensures the robustness of the proposed method. Various damaged cases and different structures are investigated in numerical simulations. From these results, anyone can point out that the present algorithm is effective and robust for structural damage identification and is also better than many other heuristic algorithms.

• Journal of Microbiology and Biotechnology
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• v.30 no.7
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• pp.1092-1096
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• 2020

YjiC, a glycosyltransferase from Bacillus licheniformis, is a well-known versatile enzyme for glycosylation of diverse substrates. Although a number of O-glycosylated products have been produced using YjiC, no report has been updated for nucleophilic N-, S-, and C- glycosylation. Here, we report the additional functional capacity of YjiC for nucleophilic N- and S- glycosylation using a broad substrate spectrum including UDP-α-D-glucose, UDP-N-acetyl glucosamine, UDP-N-acetylgalactosamine, UDP-α-D-glucuronic acid, TDP-α-L-rhamnose, TDP-α-D-viosamine, and GDP-α-L-fucose as donor and various amine and thiol groups containing natural products as acceptor substrates. The results revealed YjiC as a promiscuous enzyme for conjugating diverse sugars at amine and thiol functional groups of small molecules applicable for generating glycofunctionalized chemical diversity libraries. The glycosylated products were analyzed using HPLC and LC/MS and compared with previous reports.

• Journal of Periodontal and Implant Science
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• v.31 no.1
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• pp.233-242
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• 2001

In the treatment of chronic adult periodontitis, scaling and root planing have been generally performed prior to periodontal flap surgery. The purpose of this study was to evaluate the clinical significance of nonsurgical periodontal therapy prior to periodontal flap surgery in patients with chronic adult periodontitis. Fifty six molars showing bilateral bony defects and 4-6mm periodontal pocket in twelve patients with chronic adult periodontitis were selected. By randomized split-mouth design, in one side, flap operation was performed 4 weeks after scaling and root planing, in the other group, flap operation was only performed without scaling and root planing. Probing pocket depth, gingival recession, clinical attachment level, gingival index, plaque index, and tooth mobility were measured at baseline, before flap operation and post-operation

• Journal of the Optical Society of Korea
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• v.13 no.2
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• pp.251-255
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• 2009

In the non-contact 3-dimensional (3D) shape measurements, the fringe pattern projection method based on the phase-shifting technique has been considered very effective for its high speed and accuracy. The digital fringe projector in particular has great flexibility in generating fringe patterns since the patterns can be controlled easily by the computer program. In this work, we have developed a high-speed digital laser grating projection system using a laser diode and a polygon mirror, and evaluated its performance. It has been demonstrated that all the optical measurements required to find out the profile of a 3D object could be carried out within 31 ms, which confirmed the validity of our 3D measurement system. The result implies the more important fact that the speed in 3D measurement can be enhanced remarkably since, in our novel system, there is no device like a LCD or DMD whose response time limits the measurement speed.

• Journal of the Korean Society of Clothing and Textiles
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• v.29 no.1 s.139
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• pp.189-197
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• 2005

The purpose of this study was to find factors affecting sun protective behavior and intention to buy UV-protective clothing among Korean golfers. Health belief (HB) model and diffusion theory(DT) were used for the study. Dependent variable of HB model was sun protective behaviors (SPBs) and dependent variable of DT model was intention to buy (ITB) UV-protective clothing. Independent variables for HB model were cancer perception, perceived benefits, behavioral/psychological barriers and cues to actions, while independent variables of DT model were relative advantage, compatibility, complexity, friability, and observability, besides demographic variables. Perceived benefits and cues to action variables in addition to gender and age were significant determinants of SPB for Korean golfers. Also, relative advantage and compatibility. behavioral barriers and cues to action were significant in affecting intention to buy UV-protective clothes. Both HB model and extended DT model were useful to predict sun protective behavior of Korean golfers.