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The physico-chemical nature of prepared dextran sulfates

  • Kim, Young-Choong
    • Archives of Pharmacal Research
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    • v.4 no.1
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    • pp.33-41
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    • 1981
  • The prepared dextran sulfates were characterized by measuring the reduced viscosisty at five different concentrations to obtain an intrinsic viscosity in both phosphate and tris buffers, pH 7.4, ionic strength of 0.1 Dextran sulfates having 0.81, 1.06 sulfate groups per hexose unit have reduced viscosity value below 40 ml/g whereas dextran sulfates having 1.21, 1.43, 1.69 sulfate groups per hexose unit have reduced viscosity value over 40 ml/g. Dextran sulfate having 1.21 sulfate groups per hexose unit had highest value of reduced viscosity. The reduced viscosity of dextran sulfate in tris buffer was always higher than that in phosphate buffer regardless of the sulfate content of dextran sulfate. The influence of the sulfation of the dextran sulfate. The influence of the sulfation of the dextran sulfate molecule on the dextran sulfate-LDL interaction was studied with three different dextran sulfate molecules. Dextran sulfate molecules having more than one sulfate group per hexose unit. The dextran sulfate having 0.81 sulfate groups per hexose unit showed considerably different precipitation curves in phosphate and tris buffers. This peculiar behavior of dextran sulfate having 0.81 sulfate groups per hexose unit in the two buffer systems was not noticed with dextran sulfate having more than one noticed with dextran sulfate having more than one sulfate group per hexose unit.

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p-Cresyl sulfate and indoxyl sulfate in pediatric patients on chronic dialysis

  • Hyun, Hye Sun;Paik, Kyung Hoon;Cho, Hee Yeon
    • Clinical and Experimental Pediatrics
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    • v.56 no.4
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    • pp.159-164
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    • 2013
  • Purpose: Indoxyl sulfate and p-cresyl sulfate are important protein-bound uremic retention solutes whose levels can be partially reduced by renal replacement therapy. These solutes originate from intestinal bacterial protein fermentation and are associated with cardiovascular outcomes and chronic kidney disease progression. The aims of this study were to investigate the levels of indoxyl sulfate and p-cresyl sulfate as well as the effect of probiotics on reducing the levels of uremic toxins in pediatric patients on dialysis. Methods: We enrolled 20 pediatric patients undergoing chronic dialysis; 16 patients completed the study. The patients underwent a 12-week regimen of VSL#3, a high-concentration probiotic preparation, and the serum levels of indoxyl sulfate and p-cresyl sulfate were measured before treatment and at 4, 8, and 12 weeks after the regimen by using fluorescence liquid chromatography. To assess the normal range of indoxyl sulfate and p-cresyl sulfate we enrolled the 16 children with normal glomerular filtration rate who had visited an outpatient clinic for asymptomatic microscopic hematuria that had been detected by a school screening in August 2011. Results: The baseline serum levels of indoxyl sulfate and p-cresyl sulfate in the patients on chronic dialysis were significantly higher than those in the children with microscopic hematuria. The baseline serum levels of p-cresyl sulfate in the peritoneal dialysis group were significantly higher than those in the hemodialysis group. There were no significant changes in the levels of these uremic solutes after 12-week VSL#3 treatment in the patients on chronic dialysis. Conclusion: The levels of the uremic toxins p-cresyl sulfate and indoxyl sulfate are highly elevated in pediatric patients on dialysis, but there was no significant effect by probiotics on the reduction of uremic toxins in pediatric dialysis patients. Therefore, studies for other medical intervention to reduce uremic toxins are also necessary in pediatric patients on dialysis.

Characterization of Rhamnan Sulfate Purified from Monostroma nitidum (홑파래에서 분리정제한 Rhamnan Sulfate의 특성)

  • Bin, Jae-Hoon;Ryu, Beung-Ho
    • Korean Journal of Food Science and Technology
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    • v.28 no.5
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    • pp.859-864
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    • 1996
  • The rhamnan sulfat extracted from green algae seaweed, Monostroma nitidum was characterized on sugars, sulfate compositions and molecular structure. Rhamnan sulfate was extracted with boiling water, and purified with two steps of cetylpyridinium chloride and ion exchange chromatography. The yield of crude rhamnan sulfate was about 2% from raw material. Rhamnan sulfate fraction, F-4 was composed of 30% rhamnose, 0.9% arabinose, 2.5% xylose, 2% glucose and 32.6% sulfate. Rhamnan sulfate F-4-3 obtained from F-4 fraction was composed of 36.8% rhamnose, 3.6% xylose, 2.7% glucose, 1.4% galactose and 30.8% sulfate. The molecular weight of F-4-3 fraction was estimated as 10,000-10,300 dalton with Sephacryl S-200 gel filtration chromatography.

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Adsorption Characteristics by Synthesized Goethite in the Mixed Solution Systems of Phosphate, Sulfate, and Copper Ions (합성 Goethite에 의한 인산이온, 황산이온 및 구리이온의 혼합용액에서의 흡착특성)

  • 감상규;이동환;이민규
    • Journal of Environmental Science International
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    • v.12 no.10
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    • pp.1055-1060
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    • 2003
  • Adsorption on goethite of individual component from a solution containing phosphate, sulfate, or copper ion was investigated. Competitive adsorption in the binary and ternary solution systems composed of phosphate, sulfate, and copper ions was also investigated. In competitive adsorption systems with phosphate and sulfate ions, the presence of phosphate ion reduced the adsorption of sulfate ion largely. On the other hand, the presence of sulfate ion caused only a small decrease in phosphate adsorption. This result suggests that phosphate ion is a stronger competitor for adsorption on goethite than sulfate ion, which is consistent with the higher affinity of phosphate for the surface compared to sulfate ion. Compared to the results from single-sorbate systems, adsorption of copper ion in the binary system of sulfate ion and copper ion was found to be enhanced in the presence of sulfate ion. Addition of sulfate ion to the binary system of copper ion and phosphate ion resulted in a small enhancement in copper sorption. This result implies that the presence of sulfate ion promotes adsorption of the ternary complex FeOHCuSO$_4$. The adsorption isotherms could be well described by the Langmuir adsorption equation.

Effects of Korean Red Ginseng marc with aluminum sulfate against pathogen populations in poultry litters

  • Chung, Tae Ho;Park, Chul;Choi, In Hag
    • Journal of Ginseng Research
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    • v.39 no.4
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    • pp.414-417
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    • 2015
  • Background: The aim of this study was to evaluate the effects of Korean Red Ginseng marc with aluminum sulfate as litter amendments on ammonia, soluble reactive phosphorus, and pathogen populations in poultry litters. Methods: Increasing levels of Korean Red Ginseng marc with aluminum sulfate were applied onto the surface of rice hull as a top-dress application; untreated rice hulls served as controls. Results: Treatment with Korean Red Ginseng marc with aluminum sulfate or aluminum sulfate alone resulted in lower litter pH (p < 0.05), as compared with that of the controls. There were some differences (p < 0.05) between treatments with Korean Red Ginseng marc with aluminum sulfate or aluminum sulfate alone and controls at 2-4 wk (not at 1 wk). Ammonia levels reduced on an average by 29%, 30%, and 32% for 10 g, 20 g Korean Red Ginseng marc with aluminum sulfate, and aluminum sulfate alone, respectively, as compared with controls at 4 wk. During the experiment, Korean Red Ginseng marc with aluminum sulfate or aluminum sulfate treatment had an effect (p < 0.05) on soluble reactive phosphorus content, as compared with the controls (not at 4 wk). A decrease in Salmonella enterica and Escherichia coli was observed (p < 0.05) in litter amended with both Korean Red Ginseng marc with aluminum sulfate and aluminum sulfate alone, as compared with the control, except at 1-3 wk for Salmonella enterica and 1 wk and 4 wk for Escherichia coli, respectively. Conclusion: The results showed that using Korean Red Ginseng marc with aluminum sulfate (blends), which act as acidifying agents by reducing the pH of the litter, was equally effective as aluminum sulfate in reducing the environmental impact.

Modeling of diffusion-reaction behavior of sulfate ion in concrete under sulfate environments

  • Zuo, Xiao-Bao;Sun, Wei;Li, Hua;Zhao, Yu-Kui
    • Computers and Concrete
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    • v.10 no.1
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    • pp.79-93
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    • 2012
  • This paper estimates theoretically the diffusion-reaction behaviour of sulfate ion in concrete caused by environmental sulfate attack. Based on Fick's second law and chemical reaction kinetics, a nonlinear and nonsteady diffusion-reaction equation of sulfate ion in concrete, in which the variable diffusion coefficient and the chemical reactions depleting sulfate ion concentration in concrete are considered, is proposed. The finite difference method is utilized to solve the diffusion-reaction equation of sulfate ion in concrete, and then it is used to simulate the diffusion-reaction process and the concentration distribution of sulfate ion in concrete. Afterwards, the experiments for measuring the sulfate ion concentration in concrete are carried out by using EDTA method to verify the proposal model, and results show that the proposed model is basically in agreement with the experimental results. Finally, Numerical example has been completed to investigate the diffusion-reaction behavior of sulfate ion in the concrete plate specimen immersed into sulfate solution.

Dechlorination of Organochlorine Insecticide, Endosulfan by Zerovalent Iron (Zerovalent Iron에 의한 유기염소계 살충제 Endosulfan의 탈염소화)

  • Shin, Hyun-Su;Kim, Taek-Kyum;Kim, Jang-Eok
    • Korean Journal of Environmental Agriculture
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    • v.28 no.2
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    • pp.202-208
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    • 2009
  • The dechlorination of endosulfan (6,7,8,9,10,10-hexachloro-1,5,5a,6,9,9a-hexahydro-6,9-methano-2,4,3-benzodioxathiepine-3-oxide) and its metabolite, endosulfan sulfate via reaction with zerovalent iron under various pH conditions was studied using aqueous solution. The reaction products, which were probably produced from endosulfan and endosulfan sulfate by ZVI were identified by GC-MS. The lower the pH of reaction solution, the higher the transformation rate of endosulfan and endosulfan sulfate. The transformation rates of endosulfan and endosulfan sulfate in pH 3.0 by ZVI were 28% and 90% but those of endosulfan and endosulfan sulfate in mixture solution of water/acetone were 65% and 92%, respectively. The pH of reaction solution after ZVI treatment was increased to pH 10. Endosulfan was hydrolyzed at pH 10 but endosulfan sulfate was not hydrolyzed. Two unknown peaks were produced from endosulfan sulfate by treatment of ZVI. As a result of GC-MS analysis, unknown peaks were guessed to be structural isomer substituted hydrogen for chlorine.

A Literature Study on Self Healing Concrete Using Reaction Control Materials of Sulfate Anion (황산이온 반응제어 물질을 이용한 자기치유 콘크리트에 관한 문헌 연구)

  • Kim, Bo-Seok;Jang, Hyun-O;Lee, Han-Seung
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2016.05a
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    • pp.122-123
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    • 2016
  • Sulfate anion which cause concrete degradation is affected on marine structures. There are two of control method concrete degradation which is arisen by sulfate anion. Cementitious materials prevent permeation of sulfate anion and water-binder ratio increase to improve watertightness. But, those methods are passive. So, this study is developing new materials which prevent actively concrete degradation on sulfate anion.

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A Study on Improvement of Light Fastness on Silk-Dyed Fabric by Aftertreatment with Copper Sulfate/Thiourea (황산구리/치오요소 후처리에 의한 견직물의 일광 견뢰도 증진에 관한 연구)

  • 윤정임;김경환
    • Textile Coloration and Finishing
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    • v.5 no.2
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    • pp.117-124
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    • 1993
  • The Silk fabric was dyed with various acid dyes, and than treated with copper sulfate or copper sulfate/thiourea. Munsell color system, dye exhaustion and light fastness were investigated for the treated fabrics. The results obtaind are as follows: 1) The color variation of the silk-dyed fabric by aftertreatment with copper sulfate was shown redish, but original color with copper sulfate/thiourea. 2) Copper sulfate/thiourea was much more effective than copper sulfate only to increase the light fastness. 3) The crease recovery was increased by aftertreatment with copper sulfate/thiourea. 4) Dye exhaustion and tensile strength of the silk-dyed fabric by aftertreatment with copper sulfate was decreased about 8-15% more than those of untreated.

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Effect of Magnesium Sulfate on Sisomicin Fermentation (Sisomicin 발효에 대한 Magnesium Sulfate의 영향)

  • 한상헌;신철수
    • Microbiology and Biotechnology Letters
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    • v.20 no.2
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    • pp.213-218
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    • 1992
  • Fermentation patterns were changed by adding magnesium sulfate to the fermentation broth and its effect on enhancement of sisomicin production was investigated. When cell growth was expressed by DNA content, trophophase and idiophase were separated, but not by dry cell weight. On the other hand, addition of magnesium sulfate had the antibiotic accumulated inside the cells be liberated into the outside, and this effect resulted in improving the final antibiotic yield. The maximum antibrotic yield was obtained when 100 mM magnesium sulfate was added after one day of cultivation, and enhanced more than three times compared to that of the control to which it was not added.

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